LOCUS       BBa_K1650005            1830 bp    DNA     linear       23-Dec-2024
DEFINITION  Constitutive promoter expressing GFP and constitutive promoter 
            expressing RFP
ACCESSION   BBa_K1650005
VERSION     BBa_K1650005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
     promoter        927..961
                     /label=BBa_J23100
     misc_feature    974..977
                     /label=conserved
     RBS             970..981
                     /label=BBa_B0034
     CDS             988..1662
                     /label=mrfp1
     misc_feature    1669..1693
                     /label=Help:Barcodes
     CDS             988..1693
                     /label=BBa_E1010
     stem_loop       1713..1756
                     /label=stem_loop
     misc_feature    1702..1781
                     /label=BBa_B0010
     terminator      1702..1781
                     /label=BBa_B0010
     stem_loop       1797..1816
                     /label=T7 TE
     misc_feature    1823..1823
                     /label=stop
     polyA_site      1817..1830
                     /label=polya
     misc_feature    1790..1830
                     /label=BBa_B0012
     terminator      1790..1830
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatata ctagagttga cggctagctc agtcctaggt acagtgctag
      961 ctactagaga aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc
     1021 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
     1081 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
     1141 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
     1201 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
     1261 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
     1321 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
     1381 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac
     1441 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt
     1501 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt
     1561 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
     1621 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
     1681 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1741 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1801 caccttcggg tgggcctttc tgcgtttata
//