BBa_K1650007
type: ComponentDefinition
persistent identity: BBa K1650007
display id: BBa_K1650007
version: 1
was generated by: iGEM to SBOL conversion
description: Composite carrying the Lac-inducible CDI-operon, Lac-inducible RFP and LacI
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:02:22Z
mutable provenance: The CDI-operon was PCR amplified from genomic DNA of E.coli strain EC93. Other parts were taken from the registry.
owned by: james
owned by: myers
mutable description: The composite part contains a lac-inducible CDI operon which gives your strain the capability to inhibit growth of bacterial cells that express BamA in their outer membrane (most gram negative bacteria). Additionally the part contains mRFP1 (BBa_E1010) driven by another lac-inducible promoter as induction control. Moreover the composite part contains LacI. IPTG-induced cells harboring BBa_K1650007 express CdiB which forms a beta-barrel structure. The CdiA protein is transported through CdiB and is displayed on the cell surface. When getting in contact with BamA of a target cell, the C-terminal domain is delivered into the target cell acting as pore forming protein thus destroying the proton motoric force of the target cell. BBa_K1650007 could be used to improve transformation efficiency. Induced CDI+ cells would kill false positive cells.
discontinued: false
dominant: false
group u list: _2067_
m user id: 27248
owner id: 27248
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: BBa_K1650007 was toxic for cells in pSB1C3 backbone, presumably because of the high metabolic burden. In accordance with iGEM Headquarter, we submitted BBa_K1650007 in the low copy pSB4C5 backbone
star: false
creator: Daniel Stukenberg
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0054
component: BBa_K823008
component: BBa_E1010
component: BBa_B0034
component: BBa_R0010
component: BBa_B0053
component: BBa_K1650013
component: BBa_K1650034
component: BBa_K1650033
component: BBa_K1650003
component: BBa_B0012
component: BBa_C0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_K823008
sequence annotation: BBa_C0012
sequence annotation: BBa_B0012
sequence annotation: BBa_K1650003
sequence annotation: BBa_K1650033
sequence annotation: BBa_K1650034
sequence annotation: BBa_K1650013
sequence annotation: BBa_B0053
sequence annotation: BBa_B0054
sequence: https://synbiohub.org/public/igem/BBa_K1650007_sequence/1

BBa_B0054
type: Component
persistent identity: component2473247
display id: component2473247
version: 1
top level: BBa_K1650007
definition: BBa_B0054
access: public

BBa_K823008
type: Component
persistent identity: component2473216
display id: component2473216
version: 1
top level: BBa_K1650007
definition: BBa_K823008
access: public

BBa_E1010
type: Component
persistent identity: component2473212
display id: component2473212
version: 1
top level: BBa_K1650007
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2473209
display id: component2473209
version: 1
top level: BBa_K1650007
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2473201
display id: component2473201
version: 1
top level: BBa_K1650007
definition: LacI
access: public

BBa_B0053
type: Component
persistent identity: component2473244
display id: component2473244
version: 1
top level: BBa_K1650007
definition: BBa_B0053
access: public

BBa_K1650013
type: Component
persistent identity: component2473242
display id: component2473242
version: 1
top level: BBa_K1650007
definition: CDI
access: public

BBa_K1650034
type: Component
persistent identity: component2473241
display id: component2473241
version: 1
top level: BBa_K1650007
definition: BBa_K1650034
access: public

BBa_K1650033
type: Component
persistent identity: component2473240
display id: component2473240
version: 1
top level: BBa_K1650007
definition: BBa_K1650033
access: public

BBa_K1650003
type: Component
persistent identity: component2473239
display id: component2473239
version: 1
top level: BBa_K1650007
definition: BBa_K1650003
access: public

BBa_B0012
type: Component
persistent identity: component2473223
display id: component2473223
version: 1
top level: BBa_K1650007
definition: BBa_B0012
access: public

BBa_C0012
type: Component
persistent identity: component2473220
display id: component2473220
version: 1
top level: BBa_K1650007
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component2473218
display id: component2473218
version: 1
top level: BBa_K1650007
definition: BBa_B0034
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2473201
display id: annotation2473201
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473201/range2473201/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473201/range2473201/1
type: Range
persistent identity: range2473201
display id: range2473201
version: 1
top level: BBa_K1650007
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2473209
display id: annotation2473209
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473209/range2473209/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473209/range2473209/1
type: Range
persistent identity: range2473209
display id: range2473209
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2473212
display id: annotation2473212
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473212/range2473212/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473212/range2473212/1
type: Range
persistent identity: range2473212
display id: range2473212
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 227
end: 932
orientation: inline

BBa_K823008
type: SequenceAnnotation
persistent identity: annotation2473216
display id: annotation2473216
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473216/range2473216/1
component: BBa_K823008

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473216/range2473216/1
type: Range
persistent identity: range2473216
display id: range2473216
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 941
end: 975
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2473218
display id: annotation2473218
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473218/range2473218/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473218/range2473218/1
type: Range
persistent identity: range2473218
display id: range2473218
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 984
end: 995
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2473220
display id: annotation2473220
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473220/range2473220/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473220/range2473220/1
type: Range
persistent identity: range2473220
display id: range2473220
version: 1
top level: BBa_K1650007
direction: direction/forward
start: 1002
end: 2129
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2473223
display id: annotation2473223
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473223/range2473223/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473223/range2473223/1
type: Range
persistent identity: range2473223
display id: range2473223
version: 1
top level: BBa_K1650007
direction: direction/forward
start: 2163
end: 2203
orientation: inline

BBa_K1650003
type: SequenceAnnotation
persistent identity: annotation2473239
display id: annotation2473239
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473239/range2473239/1
component: BBa_K1650003

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473239/range2473239/1
type: Range
persistent identity: range2473239
display id: range2473239
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 2212
end: 3115
orientation: inline

BBa_K1650033
type: SequenceAnnotation
persistent identity: annotation2473240
display id: annotation2473240
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473240/range2473240/1
component: BBa_K1650033

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473240/range2473240/1
type: Range
persistent identity: range2473240
display id: range2473240
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 3124
end: 3203
orientation: inline

BBa_K1650034
type: SequenceAnnotation
persistent identity: annotation2473241
display id: annotation2473241
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473241/range2473241/1
component: BBa_K1650034

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473241/range2473241/1
type: Range
persistent identity: range2473241
display id: range2473241
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 3212
end: 3223
orientation: inline

BBa_K1650013
type: SequenceAnnotation
persistent identity: annotation2473242
display id: annotation2473242
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473242/range2473242/1
component: BBa_K1650013

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473242/range2473242/1
type: Range
persistent identity: range2473242
display id: range2473242
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 3230
end: 14643
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2473244
display id: annotation2473244
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473244/range2473244/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473244/range2473244/1
type: Range
persistent identity: range2473244
display id: range2473244
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 14652
end: 14723
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2473247
display id: annotation2473247
version: 1
top level: BBa_K1650007
location: https://synbiohub.org/public/igem/BBa_K1650007/annotation2473247/range2473247/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1650007/annotation2473247/range2473247/1
type: Range
persistent identity: range2473247
display id: range2473247
version: 1
top level: BBa_K1650007
direction: direction/reverse
start: 14732
end: 14800
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_K823008
type: ComponentDefinition
persistent identity: BBa K823008
display id: BBa_K823008
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson promoter J23106
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:05Z
mutable provenance: Partsregistry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: J23106
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823008_sequence/1

J23106
type: SequenceAnnotation
persistent identity: annotation2182530
display id: annotation2182530
version: 1
top level: BBa_K823008
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
type: Range
persistent identity: range2182530
display id: range2182530
version: 1
top level: BBa_K823008
direction: direction/forward
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182528
display id: annotation2182528
version: 1
top level: BBa_K823008
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
type: Range
persistent identity: range2182528
display id: range2182528
version: 1
top level: BBa_K823008
direction: direction/forward
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182529
display id: annotation2182529
version: 1
top level: BBa_K823008
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
type: Range
persistent identity: range2182529
display id: range2182529
version: 1
top level: BBa_K823008
direction: direction/forward
start: 24
end: 29
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_K1650033
type: ComponentDefinition
persistent identity: BBa K1650033
display id: BBa_K1650033
version: 1
was generated by: iGEM to SBOL conversion
description: T5-Promotor
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T10:57:43Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: Promotor
discontinued: false
dominant: false
group u list: _2067_
m user id: 12520
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Alexandra Richter
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_K1650033
sequence: https://synbiohub.org/public/igem/BBa_K1650033_sequence/1

BBa_K1650033
type: SequenceAnnotation
persistent identity: annotation2474906
display id: annotation2474906
version: 1
top level: BBa_K1650033
location: https://synbiohub.org/public/igem/BBa_K1650033/annotation2474906/range2474906/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1650033/annotation2474906/range2474906/1
type: Range
persistent identity: range2474906
display id: range2474906
version: 1
top level: BBa_K1650033
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_K1650003
type: ComponentDefinition
persistent identity: BBa K1650003
display id: BBa_K1650003
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive promoter expressing RFP
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T03:47:22Z
mutable provenance: All parts were taken from the Registry.
owned by: james
owned by: myers
mutable description: Constitutive Promoter from promoter family of the Berkeley Team 2006 expressing GFP.
discontinued: false
dominant: false
experience: experience/None
group u list: _2067_
m user id: 27864
owner id: 27864
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Sequence verifying before using to ensure correct sequence in order to use advanced cloning techniques.
star: false
creator: Anna Knoerlein
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_E1010
component: BBa_B0034
component: BBa_J23101
component: BBa_B0012
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1650003_sequence/1

BBa_B0010
type: Component
persistent identity: component2470294
display id: component2470294
version: 1
top level: BBa_K1650003
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component2470293
display id: component2470293
version: 1
top level: BBa_K1650003
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2470290
display id: component2470290
version: 1
top level: BBa_K1650003
definition: BBa_B0034
access: public

BBa_J23101
type: Component
persistent identity: component2470288
display id: component2470288
version: 1
top level: BBa_K1650003
definition: BBa_J23101
access: public

BBa_B0012
type: Component
persistent identity: component2470296
display id: component2470296
version: 1
top level: BBa_K1650003
definition: BBa_B0012
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2470288
display id: annotation2470288
version: 1
top level: BBa_K1650003
location: https://synbiohub.org/public/igem/BBa_K1650003/annotation2470288/range2470288/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1650003/annotation2470288/range2470288/1
type: Range
persistent identity: range2470288
display id: range2470288
version: 1
top level: BBa_K1650003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2470290
display id: annotation2470290
version: 1
top level: BBa_K1650003
location: https://synbiohub.org/public/igem/BBa_K1650003/annotation2470290/range2470290/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1650003/annotation2470290/range2470290/1
type: Range
persistent identity: range2470290
display id: range2470290
version: 1
top level: BBa_K1650003
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2470293
display id: annotation2470293
version: 1
top level: BBa_K1650003
location: https://synbiohub.org/public/igem/BBa_K1650003/annotation2470293/range2470293/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1650003/annotation2470293/range2470293/1
type: Range
persistent identity: range2470293
display id: range2470293
version: 1
top level: BBa_K1650003
direction: direction/reverse
start: 62
end: 767
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2470294
display id: annotation2470294
version: 1
top level: BBa_K1650003
location: https://synbiohub.org/public/igem/BBa_K1650003/annotation2470294/range2470294/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1650003/annotation2470294/range2470294/1
type: Range
persistent identity: range2470294
display id: range2470294
version: 1
top level: BBa_K1650003
direction: direction/forward
start: 776
end: 855
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2470296
display id: annotation2470296
version: 1
top level: BBa_K1650003
location: https://synbiohub.org/public/igem/BBa_K1650003/annotation2470296/range2470296/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1650003/annotation2470296/range2470296/1
type: Range
persistent identity: range2470296
display id: range2470296
version: 1
top level: BBa_K1650003
direction: direction/forward
start: 864
end: 904
orientation: inline

BBa_K1650034
type: ComponentDefinition
persistent identity: BBa K1650034
display id: BBa_K1650034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T10:59:15Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: (T5)
discontinued: false
dominant: false
group u list: _2067_
m user id: 12520
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Alexandra Richter
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: BBa_K1650034
sequence: https://synbiohub.org/public/igem/BBa_K1650034_sequence/1

BBa_K1650034
type: SequenceAnnotation
persistent identity: annotation2474905
display id: annotation2474905
version: 1
top level: BBa_K1650034
location: https://synbiohub.org/public/igem/BBa_K1650034/annotation2474905/range2474905/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1650034/annotation2474905/range2474905/1
type: Range
persistent identity: range2474905
display id: range2474905
version: 1
top level: BBa_K1650034
direction: direction/forward
start: 1
end: 12
orientation: inline

CDI
type: ComponentDefinition
persistent identity: BBa K1650013
display id: BBa_K1650013
version: 1
was generated by: iGEM to SBOL conversion
description: This part contains the CDI operon
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:47:35Z
mutable provenance: asdljasd
owned by: james
owned by: myers
mutable description: asldk
discontinued: false
dominant: false
experience: experience/Works
group u list: _2067_
m user id: 27248
owner id: 27248
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: asdkljal
star: false
creator: Daniel Stukenberg
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CdiB
sequence annotation: intergenic region
sequence annotation: CdiA
sequence annotation: CdiI
sequence: https://synbiohub.org/public/igem/BBa_K1650013_sequence/1

CdiB
type: SequenceAnnotation
persistent identity: annotation2473449
display id: annotation2473449
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473449/range2473449/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473449/range2473449/1
type: Range
persistent identity: range2473449
display id: range2473449
version: 1
top level: CDI
direction: direction/forward
start: 1
end: 1767
orientation: inline

intergenic region
type: SequenceAnnotation
persistent identity: annotation2473450
display id: annotation2473450
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473450/range2473450/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473450/range2473450/1
type: Range
persistent identity: range2473450
display id: range2473450
version: 1
top level: CDI
direction: direction/forward
start: 1768
end: 1779
orientation: inline

CdiA
type: SequenceAnnotation
persistent identity: annotation2473452
display id: annotation2473452
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473452/range2473452/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473452/range2473452/1
type: Range
persistent identity: range2473452
display id: range2473452
version: 1
top level: CDI
direction: direction/forward
start: 1780
end: 11178
orientation: inline

CdiI
type: SequenceAnnotation
persistent identity: annotation2473451
display id: annotation2473451
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473451/range2473451/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473451/range2473451/1
type: Range
persistent identity: range2473451
display id: range2473451
version: 1
top level: CDI
direction: direction/forward
start: 11176
end: 11416
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

https://synbiohub.org/public/igem/BBa_K1650007_sequence/1
type: Sequence
persistent identity: BBa K1650007 sequence
display id: BBa_K1650007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1650033_sequence/1
type: Sequence
persistent identity: BBa K1650033 sequence
display id: BBa_K1650033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatcataaaaaatttatctgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823008_sequence/1
type: Sequence
persistent identity: BBa K823008 sequence
display id: BBa_K823008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1650003_sequence/1
type: Sequence
persistent identity: BBa K1650003 sequence
display id: BBa_K1650003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1650034_sequence/1
type: Sequence
persistent identity: BBa K1650034 sequence
display id: BBa_K1650034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaggaatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1650013_sequence/1
type: Sequence
persistent identity: BBa K1650013 sequence
display id: BBa_K1650013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcggcaccggcaggataacttactggcaaacagaacattgttgcccggtatggcttccggtcagtacgtattcaggctctgcactttctctccggtggtacgctatttttccctcctcccctgcctttgtattctttcgttttcgtctccggcagccatgctgtctccgggtgaccgcagtgcaattcagcagcaacagcagcagttgctggatgaaaaccagcgtcagcgtgatgcgctggagcgcagtgcgccgctgaccatcacgccgtctccggaaacgtctgccggtactgaaggtccctgctttacggtgtcaagcattgttgtcagtggggccacccgactgacgtctgcagaaaccgacagactggtggcaccgtgggtgaatcagtgtctgaatatcacggggctgaccgcggtcacggatgccatgacggacagctatatacgccggggatatatcaccagccgggcctttctgacagagcaggacctttcagggggcgtactgcacataacggtcatggaaggcaggctgcagcaaatccgggcggaaggcgctgaccttcctgcccgcaccctgaagatggttttcccgggaatggaggggaaggttctgaacctgcgggatattgagcaggggatggagcagattaatcgtctgcgtacggagccggtacagattgaaatatcgcccggtgaccgtgagggatggtcggtggtgacactgacggcattgccggaatggcctgtcacagggagcgtgggcatcgacaacagcgggcagaagagtaccggtacggggcagttaaatggtgtcctttcctttaataatcctctggggctggctgacaactggtttgtcagcgggggacggagcagtgacttttcggtgtcacatgatgcgaggaattttgccgccggtgtcagtctgccgtatggctataccctggtggattacacgtattcatggagtgactacctcagcaccattgataaccggggctggcggtggcgttccacgggagacctgcagactcaccggctgggactgtcgcatgtcctgttccgtaacggggacatgaagacagcactgaccggaggtctgcagcaccgcattattcacaattatctggatgatgttctgcttcagggcagcagccgtaaactcacttcattttctgtcgggctgaatcacacccacaagtttctggggggggtcggaacactgaatccggtattcacacgggggatgccctggttcggcgcagaaagcgaccacgggaaaaggggagacctgcccgtaaatcagttccggaaatggtcggtgagtgccagttttcagcgccccgtcacggacagggtgtggtggctgaccagcgcttatgcccagtggtcaccggaccgtcttcatggtgtggaacaactgagcctcgggggtgagagttcagtgcgtggctttaaggatcagtatatctccggtaataacggcggttatctgcggaatgagctgtcctggtctctgttctccctgccatatgtgggaactgtccgtgcagtggctgcactggacggcggctggctgcactctgacagcgatgacccgtactcgtccggcacgctgtggggtgctgctgccgggctcagcaccaccagtggccatgtttccggttcgttcactgccggactgcctctggtttacccggactggcttgcccctgaccatctcacggtttactggcgcgttgccgtcgcgttttaagggattattaccatgcatcagcctcccgttcgcttcacttaccgcctgctgagttacctggtcagtgcgattatcgccgggcagccgttgttaccggctgtgggggccgtcatcaccccacaaaacggggccggaatggataaagcggcaaatggtgtgccggtcgtgaacattgccacgccgaacggggccgggatttcgcataaccggtttacggattacaacgtcgggaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z