LOCUS BBa_K1650009 12544 bp DNA linear 22-Nov-2024 DEFINITION Composite carrying the Lac-inducible CDI-operon,constitutively expressed RFP ACCESSION BBa_K1650009 VERSION BBa_K1650009.1 FEATURES Location/Qualifiers misc_feature 1..6 /label=-35 box misc_feature 24..29 /label=-10 box promoter 1..35 /label=J23106 promoter 1..35 /label=BBa_K823008 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..736 /label=mrfp1 misc_feature 743..767 /label=Help:Barcodes CDS 62..767 /label=BBa_E1010 stem_loop 787..830 /label=stem_loop misc_feature 776..855 /label=BBa_B0010 terminator 776..855 /label=BBa_B0010 promoter 864..943 /label=BBa_K1650033 promoter 864..943 /label=BBa_K1650033 RBS 952..963 /label=BBa_K1650034 RBS 952..963 /label=BBa_K1650034 CDS 970..2736 /label=CdiB CDS 2737..2748 /label=intergenic region CDS 12145..12385 /label=CdiI CDS 2749..12147 /label=CdiA CDS 970..12387 /label=BBa_K1650013 stem_loop 12404..12438 /label=stem_loop terminator 12396..12467 /label=BBa_B0053 stem_loop 12486..12517 /label=stem loop misc_feature 12479..12518 /label=identical to E. coli MG1655 between tonB and yciA terminator 12476..12544 /label=BBa_B0054 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 121 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 181 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 241 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 301 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 361 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 421 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 481 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 541 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 601 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 661 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 721 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 841 tcggtgaacg ctctctacta gagaaatcat aaaaaattta tctgctttgt gagcggataa 901 caattataat agattcaatt gtgagcggat aacaatttca cactactaga gaaggaggaa 961 ttatactaga tgcggcaccg gcaggataac ttactggcaa acagaacatt gttgcccggt 1021 atggcttccg gtcagtacgt attcaggctc tgcactttct ctccggtggt acgctatttt 1081 tccctcctcc cctgcctttg tattctttcg ttttcgtctc cggcagccat gctgtctccg 1141 ggtgaccgca gtgcaattca gcagcaacag cagcagttgc tggatgaaaa ccagcgtcag 1201 cgtgatgcgc tggagcgcag tgcgccgctg accatcacgc cgtctccgga aacgtctgcc 1261 ggtactgaag gtccctgctt tacggtgtca agcattgttg tcagtggggc cacccgactg 1321 acgtctgcag aaaccgacag actggtggca ccgtgggtga atcagtgtct gaatatcacg 1381 gggctgaccg cggtcacgga tgccatgacg gacagctata tacgccgggg atatatcacc 1441 agccgggcct ttctgacaga gcaggacctt tcagggggcg tactgcacat aacggtcatg 1501 gaaggcaggc tgcagcaaat ccgggcggaa ggcgctgacc ttcctgcccg caccctgaag 1561 atggttttcc cgggaatgga ggggaaggtt ctgaacctgc gggatattga gcaggggatg 1621 gagcagatta atcgtctgcg tacggagccg gtacagattg aaatatcgcc cggtgaccgt 1681 gagggatggt cggtggtgac actgacggca ttgccggaat ggcctgtcac agggagcgtg 1741 ggcatcgaca acagcgggca gaagagtacc ggtacggggc agttaaatgg tgtcctttcc 1801 tttaataatc ctctggggct ggctgacaac tggtttgtca gcgggggacg gagcagtgac 1861 ttttcggtgt cacatgatgc gaggaatttt gccgccggtg tcagtctgcc gtatggctat 1921 accctggtgg attacacgta ttcatggagt gactacctca gcaccattga taaccggggc 1981 tggcggtggc gttccacggg agacctgcag actcaccggc tgggactgtc gcatgtcctg 2041 ttccgtaacg gggacatgaa gacagcactg accggaggtc tgcagcaccg cattattcac 2101 aattatctgg atgatgttct gcttcagggc agcagccgta aactcacttc attttctgtc 2161 gggctgaatc acacccacaa gtttctgggg ggggtcggaa cactgaatcc ggtattcaca 2221 cgggggatgc cctggttcgg cgcagaaagc gaccacggga aaaggggaga cctgcccgta 2281 aatcagttcc ggaaatggtc ggtgagtgcc agttttcagc gccccgtcac ggacagggtg 2341 tggtggctga ccagcgctta tgcccagtgg tcaccggacc gtcttcatgg tgtggaacaa 2401 ctgagcctcg ggggtgagag ttcagtgcgt ggctttaagg atcagtatat ctccggtaat 2461 aacggcggtt atctgcggaa tgagctgtcc tggtctctgt tctccctgcc atatgtggga 2521 actgtccgtg cagtggctgc actggacggc ggctggctgc actctgacag cgatgacccg 2581 tactcgtccg gcacgctgtg gggtgctgct gccgggctca gcaccaccag tggccatgtt 2641 tccggttcgt tcactgccgg actgcctctg gtttacccgg actggcttgc ccctgaccat 2701 ctcacggttt actggcgcgt tgccgtcgcg ttttaaggga ttattaccat gcatcagcct 2761 cccgttcgct tcacttaccg cctgctgagt tacctggtca gtgcgattat cgccgggcag 2821 ccgttgttac cggctgtggg ggccgtcatc accccacaaa acggggccgg aatggataaa 2881 gcggcaaatg gtgtgccggt cgtgaacatt gccacgccga acggggccgg gatttcgcat 2941 aaccggttta cggattacaa cgtcgggaag gaagggctga ttctcaataa tgccaccggt 3001 aagcttaatc cgacgcagct tggtggactg atacagaata acccgaacct gaaagcgggc 3061 ggggaagcga agggtatcat caacgaagtg accggcggta agcgttcact gctgcagggc 3121 tatacggaag tggccggcaa agcggcgaat gtgatggttg ccaacccgta tggtatcacc 3181 tgtgacggct gtggctttat caacacgccg cacgcgacgc tcaccacggg caaacctgtg 3241 atgaatgccg acggcagcct gcaggcgctg gaggtgactg aaggcagtat caccatcaat 3301 ggcgcgggcc tggacggcac ccggagcgat gccgtatcca ttattgcccg tgcaacggaa 3361 gtgaatgccg cgcttcatgc gaaggattta actgtcactg caggcgctaa ccggataact 3421 gcagatggtc gcgtcagtgc cctgaagggc gaaggtaatg tgccgaaagt tgccgttgat 3481 accggcgctc tcggtggaat gtacgccagg cgtattcatc tgacctccac tgaaagtggt 3541 gtcggggtta atcttggtaa cctttatgcc cgcgagggcg atatcatact gagcagttcc 3601 ggaaaactgg tcctgaagaa cagccttgcc ggcggcaata ccaccgtaac cggaacggat 3661 gtctcacttt caggggataa caaagccgga ggaaatctca gcgttaccgg gacaacggga 3721 ctgacactga atcagtcccg tctggtgacg gataaaaatc tggtgctgtc ttcatccggg 3781 cagattgtac agaacggtgg tgaactgact gccgggcaga acgccatgct cagtgcacag 3841 cacctgaacc agacttccgg gaccgtgaat gcagctgaaa atgtcacctt gaccaccacc 3901 gatgatacca cactgaaagg ccgcagcgtt gccgggaaaa cactcaccgt cagttccggc 3961 agcctgaaca acggtgggac actggttgcc gggcgcgatg ccacggtgaa aaccgggaca 4021 ttcagtaata ccggtaccgt ccaggggaat ggcctgaaag ttaccgccac tgacctgacc 4081 agcaccggca gtattaaaag tggcagcaca ctcgatatca gcgcccgcaa tgccacactg 4141 tccggtgatg ccggagcaaa agacagagcc ctcgttaccg tcagcggtac acttgaaaac 4201 cgcggcagac tcgtcagcga tgacgtgctg acgctcagtg ccacgcagat aaacaacagc 4261 ggtaccctct ccggggcaaa ggaacttgtg gcttctgcag acacactgac caccacagaa 4321 aaatcggtca caaacagtga cggtaacctc atgctggaca gcgcgtcttc cacactggcg 4381 ggtgaaacca gtgcgggtgg cacggtgtct gtaaaaggca acagtctgaa gaccacgacc 4441 actgcgcaga cgcagggcaa cagtgtcagc gtggatgtgc agaacgcaca gcttgacgga 4501 acacaggctg ccagagacat ccttaccctg aacgccagtg aaaagctcac ccacagcggg 4561 aaaagcagtg ccccgtcgct cagcctcagt gcgccggaac tgaccagcag cggcgtactt 4621 gttggttccg ccctgaatac acagtcacag accctgacca acagcggtct gttgcagggg 4681 aaggcctcac tcaccgttaa cacacagagg cttgataatc agcagaacgg cacgctgtac 4741 agtgctgcag acctgacgct ggatataccg gacatccgca acagcgggct tatcaccggt 4801 gataatggtt taatgttaaa tgctgtctcc ctcagcaatc cgggaaaaat catcgctgac 4861 acgctgagcg tcagggcgac cacgctggat ggtgacggcc tgttgcaggg cgccggtgca 4921 ctggcgcttg caggtgacac actctcactg ggtagtaacg gacgctggct gacggcgggc 4981 gacctctccc tccggggtaa aacactgcat accgcaggga ccacgcaggg acagaatctc 5041 accgtgcagg cggacagatg ggcgaacagt ggttccgtgc aggcaaccgg taaccttact 5101 gcttcggcaa ccggtcagtt gaccagtacc ggcgatatca tgagccaggg tgacaccacg 5161 ctgaacgcag ccaccacgga caaccggggc agtctgcttt cggccggcac gctctccctt 5221 gatggaaact cactggataa cagcggcact gtccagggta accatgtcac gattcgccag 5281 aacggtgtca ccaacagtgg cacgctcacc gggatcgccg cgctgacgct tgccgcccgt 5341 atggatatgg catcccctca acctgcgctg atgaataacg gaggttcatt gctgaccagc 5401 ggcgatctga caatcaccgc aggcagtctg gcaaacagcg gggcgatcca ggcggctgac 5461 agcctgactg cacgtctgac gggtgagctc gtcagcacag caggcagcaa agtcacctcg 5521 aacggtgaaa tggcgctcag tgcactgaat ttaagcaaca gcggacaatg gattgcaaaa 5581 aatctgactc tgaaggcgaa ctcactgacc agtgcgggtg acatcaccgg tgtggatgct 5641 ctcacgctca cggtgaatca gacgctgaac aatcacgcga gcggaaaact gctcagtgca 5701 ggtgtgctga cgctgaaggc agacagtgtc aaaaacgacg ggcaattaca gggaaatgcc 5761 accaccatca cggcaggaca actcacaaac ggcgggcatc tgcagggcga aacgctgacg 5821 ctggccgcct ccggtggcgt gaacaaccgt tccggtggtg ttctgatgag ccggaatgca 5881 ctgaatgtca gtactgcgac cctgagtaac cagggcacga tacagggtgg tggcggggtt 5941 tccctgaacg ccactgaccg tctgcagaac gacggcaaaa tcctctccgg cagtaacctg 6001 acgctgacgg cgcaggtgct ggcgaacacc ggcagcggac tggtacaggc tgccaccctg 6061 ctgctggatg tggtgaatac tgtcaacggc ggacgcgtac ttgccaccgg cagtgccgac 6121 gttaaaggaa ccacgctgaa taataccggt acgtttcagg gggcggacct gctggtgaat 6181 taccacacat tcagcaacag cggtaccctg ctgggaacct ccgggcttgg cgtcaagggc 6241 agttcactgc tgcaaaatgg tacagggcgg ctgtacagtg caggcaacct gctgcttgac 6301 gctcaggact tcagtggtca gggccaggtg gtggccaccg gtgatgtcac actgaaactg 6361 attgctgccc tcacgaatca tggtaccctg gccgcaggga aaaccctttc cgtcacgtcg 6421 cagaacgccg tcaccaacgg cggtgtgatg cagggtgatg ccatggtgct cggtgccgga 6481 gaggcattca ccaacaatgg aacgctgact gccggtaaag gcaacagtgt tttcagcgca 6541 cagcgtcttt tccttaacgc accgggttca cttcaggccg gtggcgatgt gagtctgaac 6601 agccggagtg atatcaccat cagtggtttt accggcacgg caggcagtct gacaatgaat 6661 gtggccggta ccctgctgaa cagtgcgctg atttatgcgg ggaataacct gaagctgttt 6721 acagaccgtc tgcataacca gcatggtgat atcctggccg gcaacagtct gtgggtacag 6781 aaggacagca gcggtacggc aaacagcgaa atcattaacc gttccggtaa tattgaaacc 6841 acacgtggcg atatcaccat gaatacggcg catctgctga attcatggga tgccatttcg 6901 gccagccatg aggtcattcc tggaagcagt cacggggtga tttcaccggt tccggaaaat 6961 aaccggtggt ggggtgttgt ccggcatgat ggtgtggaat atctggccgt gtactggggt 7021 aagggtgcca cggtacctga tgaataccgt atccgtacgg gagatacgga aacggtgaca 7081 gtcagtgcct ccggccatgc tgccaggata tccggcggcg ctgatatgca tatccgtgca 7141 ggacgactgg ataatgaggc atcgtttatt ctcgccggtg gcggtatgac cctgagtggc 7201 gacacactga acaatcaggg ctggcaggag gggaccaccg gaaaggaaac ggtctggcgg 7261 cttgcctcag gctcactccc caaagcatgg tttactgagc catggtataa ggtctatcgc 7321 caggtctcac cggatgcgac agaagccagt ggcacctctc cggcaggtca gtaccgtgcg 7381 gttatcagcg ctgccggtga tgtatctgcc agctttgcca ctgacaccgg taataccact 7441 gtcatgcccc gggcaggtgg tgcaggtaat acgataacgg tgccttcgct gaactccctg 7501 acaccaccga cggtcagtca gggggtgagc ggtgaagccc tgctgaatga aagcggtacc 7561 gggataaccg gccctgtatg gaatgacgcg ctgccggata cgctgaagga tatcccgggt 7621 gcgctgtcgc tgtccggggc aagtgtaagc agctatcccc tgccatccgg taacaatggt 7681 tattttgtcc cgtccacgga cccggacagt ccgtatctga ttacggtgaa cccgaaactg 7741 gatggtctcg gaaaggtgga cagcagtctg tttgccggac tgtatgacct gctcaggatg 7801 caacccggag aggcaccacg tgaaacagac ccggcatata ccgatgaaaa acagtttctg 7861 ggctcgtcgt acattcttga ccgtcttggc ctgaaaccgg aaaaagatta ccgctttctg 7921 ggggatgcgg cttttgatac ccggtatgtc agtaacgtca tactgaacca gacgggctca 7981 cgttatatca acggcacagg ttctgacctg gcgcagatga aatacctgat ggacagcgcg 8041 gcagcacaac agaaagcgct cggactgacc tttggtgtgt cgctgaccgc cgggcaggtt 8101 gcacaactga ccagaagcct gctgtggtgg gagtccgtca ccatcaacgg acagacagtc 8161 atggttccga aactgtatct gtctccggaa gatatcaccc ttcataatgg cagcgttatc 8221 agcgggaaca acgtgcagct tgcgggtggc aatatcacca acagcggcag cagcatcaac 8281 gcacagaatg acctcctgct cgacagaaca ggtagtatcg acaacctgaa tgccggactg 8341 ataaacgccg gtggtgcact gaacctgaag gccatcgggg atatcggcaa tatcagctca 8401 gtcatcagcg gtaaaaccgt atccctggaa agcgccaccg gaaacatcag taatctcacc 8461 cggacagaac agtgggcgat gaacaacgga tataaccatt tcagcggaac ggataccggt 8521 ccgctggctg ctgtcagggc aacggactca ctgtttatgg gcgcagccgg agatatcagt 8581 atcaccggtg ccgccgtttc tgccggtgac agcgtgttac tggccgccgg taatgacctg 8641 aacatgaacg cgatacaggc cggagaacgt cgtcgctatg gcgggtccgg gtggtatgaa 8701 actcatgccg tggcccccac ggtcactgcc gggaacagtc tgatgctttc cgccggtcgt 8761 gatgtgaaca gccaggctgc cggtattaca gctgaaaaca gtatggatat ccgcgccgga 8821 cgggatgtga acatggctgc ggagtccaca ggcgcaggtg accacgacag cactttcagc 8881 atgaaaacgg ttcatgattc ggtccggcag cagggaacag acatgaccag cggcggtgac 8941 atcacggtta ctgccggacg ggatatcacc tcagttgcga ctgctgtaac ggcaaagggc 9001 gatatccgtg tgaatgccgg tcatgacatc gtactgggta ctgccacgga aagtgattat 9061 cactacagtg aatcagggga aacccgtaac cgtctcctca gccatcagac cacccggacc 9121 atcacggaag acagcgtcac ccgtgagaag ggctccctgc tgtcgggaaa ccgcgtgacc 9181 gttaacgccg gtaataacct gacggtacag ggttcggatg tggtggctga ccgggatgtg 9241 tcactggcgg cggataacca tgttgatgtt cttgctgcca ccagtacaga tacgtcctgg 9301 cgctttaagg aaacgaagac atccggtctg acgggtaccg gcggtattgg tttcaccact 9361 ggcagcagta agacaacgca cgaccgccgc gaggccggga caacgcagag tcagagtgcc 9421 agtaccatcg gctccactgc cggtaatgtc agtattaccg cgggcaaaca ggctcatatc 9481 agcggttcgg atgtgattgc gaaccgggat atcagcatta ccggtgacag tgtggtggtt 9541 gacccggggc atgaccgtcg tactgtggac gaaaaatttg agcagaagaa aagcgggctg 9601 acggttgccc tgtccggtgc ggtgggtagt gccatcaata atgcggtcac catggcccgg 9661 gaggcgaaag aaaccagcga cagccgtctg gcggcactga aaggaacaca ggcggttctg 9721 tctggtgtac aggccggtgt taaccacgga ctgcagcaac agagtgctga cccgaataat 9781 ggtatcggcg tgagcatttc gctcaaccat cagcagtcga agtctgagac gaaatatcag 9841 cacgatatcg tctccggctc aaccctcagc gccggtaaca atgtctctgt cactgccacg 9901 gggaaaaata aggaccataa caacagcggc gatatgctca ttaccgggag ccagattaag 9961 tccggcaatg acaccagcct gaatgcacag aacgatattc tgctggctgc cgccgctgac 10021 acccgacaga caaccgggaa gaacagcagc aaaggtggcg gcgtgggcgt cagctttggt 10081 ggtggaacca atggcggagg cctcagtatc ttcgccggta tcaatggttc agagggcagg 10141 gagaaaggca acggcacgac ctggaccgaa accacgctgg atgcgggtaa aaatgtctcc 10201 ctcacaagcg gacgtgatac caccctgtcg ggggctcagg tcagtggcga gaaagtcaca 10261 gcagatgtcg gcaacaacct gaccatttcg agccttcagg acagtgaccg ttacgacagc 10321 agacagaacc gcgtggccgc aggtggcagc ttcacctttg gctcgatgtc cggcagcggc 10381 tatgccagca tcagccagga caaaattaaa agcaattacg attctgtccg ggaacagagt 10441 ggtatctacg ccggaaaaga cgggtttgat gttactgtgg ggaatcacac tcagctcaat 10501 ggtgcggtaa ttgcgtccac ggcaacggat gacaaaaaca gcctcaatac caacacgctg 10561 ggctggtcgg atatccataa ccaggcagac tataaagcca gccataccgg aatcagttta 10621 tcgggtggct cagggatgtc agccagccag atggtggcct caaatgcaat agccggtgcg 10681 gcgaacgcac ttaccggtat gtccggcagc agtggacatg ctgaaggtac cacgtcatct 10741 gccatcagcg gcggaaatct catcatccgt aataaggaga gtcagaagca ggatattgcc 10801 gggctcagcc gggacccgga aaacgccaac ggcagcattg caccgatatt tgacagggag 10861 aaagagcaga agcgtcttca ggaagcgcag gttatcagcc agattagtgg tcagatgagt 10921 aacatcgtca tgacgtatgg cgaaactgaa gcgatgaaag cagccaggaa agaacatcct 10981 ggcatgagcg atgcacagtt gcgggagacc ccggaataca gagaggtcat gaaaggctac 11041 ggtacgggca gtacgccgca gatggtggta caggccatca cgggcgtgct gggcggactg 11101 aatgcaggga atccggggca ggtactggct ggcggtctga atccggcagt ggcacagctt 11161 ataaaacagg ctacaggcga taaccgggaa gcaaacctga tggctcatgc agtctgggga 11221 gcacttgccg cacaactggg cggaaacaat gcggcttccg gtgctgccgg ggcattcagt 11281 ggtgaacttg cagcccggta tatcattgat aactactacg gtggtcgtac cgataacctg 11341 agtgaacagg aacgtcagca aatcagcatg ctggccacca ttgcttcagg tattgcaggc 11401 gggcttgtcg gcaacagtac aagtgctgcc ggtacggggg cacaggcagg taggaactcg 11461 gttgagaata atgcaatgtc tggcctggaa gggtttggga ccggcttcca gagttatgtt 11521 caggcccagg aggcgctggt aaataatacc aatcttactg ataaaaatgg caaagtactg 11581 aatccggcaa caccagagga aattaagtac gcctctgata agctggtaac cggttctata 11641 cctgaaggac aggaccctgc cagaggttta ctgatttcat ggggagccgg agcatctgtt 11701 tttgggggag aacttatagc tccagcagtt ggcacggttg ctgttatcgg aggtaccctc 11761 ttaggtggta ctacagatgc tgttaagcag tttttgaccc tgaaaccggg tgagcaatac 11821 agtactacag atactcttat tgccgcaggt gaaggtgggc ttactcaggg gaaaggggtc 11881 atcttcagta catttattaa cacaatgggg gcttacttag gaagtaaagc taaaggggaa 11941 gaccctacag ggccaatggt gggaaatgct ataggtactg cactgggaaa taaagccgga 12001 gataaattca caaaagaaat gctctcaaga ggctttggct ctgtcacatc agaggttaca 12061 ggtacagtta ctggtagtgt tattgggaca gtgactgatt atcagatcga aaagctagga 12121 aaaggtaata aggaaggggc aaaatgaatg aagaagaaac tatttgccct tttaaaatat 12181 attatttttt ttcctatgct atgtactgta cttggtcttt taggtatacc tataggttta 12241 attgtgaatt ttttgagaac gggctctttt gacttcaact taaaagatga aattgatgtt 12301 gttttattca ctttaaaaat aggaatcccc ataggcttta ttcttgggct gggtttgtgg 12361 ggccttagta tcttagacag aaaatagtac tagagtccgg caaaaaaacg ggcaaggtgt 12421 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgtttac tagagattag 12481 cagaaagtca aaagcctccg accggaggct tttgactaaa acttcccttg gggttatcat 12541 tggg //