BBa_K823008 1 BBa_K823008 Anderson promoter J23106 2012-09-07T11:00:00Z 2015-07-07T11:47:05Z Partsregistry Released HQ 2013 Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon. false false _1081_ 4206 12081 9 In stock false no considerations false Korinna Kraft annotation2182530 1 J23106 range2182530 1 1 35 annotation2182528 1 -35 box range2182528 1 1 6 annotation2182529 1 -10 box range2182529 1 24 29 BBa_B0053 1 BBa_B0053 Terminator (His) 2004-01-29T12:00:00Z 2015-08-31T04:07:20Z Terminator from the E.coli His operon false true _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318602 1 stem_loop range318602 1 9 43 BBa_B0054 1 BBa_B0054 Transcriptional terminator 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783342 1 identical to E. coli MG1655 between tonB and yciA range1783342 1 4 43 annotation1747359 1 stem loop range1747359 1 11 42 BBa_K1650013 1 CDI This part contains the CDI operon 2015-09-17T11:00:00Z 2015-09-18T11:47:35Z asdljasd asldk false false _2067_ 27248 27248 9 true asdkljal false Daniel Stukenberg annotation2473451 1 CdiI range2473451 1 11176 11416 annotation2473450 1 intergenic region range2473450 1 1768 1779 annotation2473449 1 CdiB range2473449 1 1 1767 annotation2473452 1 CdiA range2473452 1 1780 11178 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1650034 1 BBa_K1650034 RBS 2015-09-17T11:00:00Z 2015-09-18T10:59:15Z test (T5) false false _2067_ 12520 27246 9 false test false Alexandra Richter annotation2474905 1 BBa_K1650034 range2474905 1 1 12 BBa_K1650033 1 BBa_K1650033 T5-Promotor 2015-09-17T11:00:00Z 2015-09-18T10:57:43Z test Promotor false false _2067_ 12520 27246 9 false test false Alexandra Richter annotation2474906 1 BBa_K1650033 range2474906 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1650009 1 BBa_K1650009 Composite carrying the Lac-inducible CDI-operon,constitutively expressed RFP 2015-09-17T11:00:00Z 2015-09-19T12:10:04Z The CDI-operon was PCR amplified from genomic DNA of E.coli strain EC93. Other parts were taken from the registry. Note: This part is similar to BBa_K1650007 except that it does not contain lacI and RFP expression is driven by a constitutive promoter instead of a lac-inducible promoter. The composite part contains a lac-inducible CDI operon which gives your strain the capability to inhibit growth of bacterial cells that express BamA in their outer membrane (most gram negative bacteria). Additionally the part contains mRFP1 (BBa_E1010) driven by constitutive promoter. IPTG-induced cells harboring BBa_K1650009 express CdiB which forms a beta-barrel structure. The CdiA protein is transported through CdiB and is displayed on the cell surface. When getting in contact with BamA of a target cell, the C-terminal domain is delivered into the target cell acting as pore forming protein thus destroying the proton motoric force of the target cell. BBa_K1650009 could be used to improve transformation efficiency. Induced CDI+ cells would kill false positive cells false false _2067_ 27248 27248 9 false BBa_K1650009 was toxic for cells in pSB1C3 backbone, presumably because of the high metabolic burden. In accordance with iGEM Headquarter, we submitted BBa_K1650009 in the low copy pSB4C5 backbone false Daniel Stukenberg component2476113 1 BBa_B0010 component2476116 1 BBa_K1650033 component2476125 1 BBa_B0053 component2476123 1 BBa_K1650013 component2476118 1 BBa_K1650034 component2476112 1 BBa_E1010 component2476128 1 BBa_B0054 component2476107 1 BBa_K823008 component2476109 1 BBa_B0034 annotation2476116 1 BBa_K1650033 range2476116 1 864 943 annotation2476112 1 BBa_E1010 range2476112 1 62 767 annotation2476123 1 BBa_K1650013 range2476123 1 970 12387 annotation2476109 1 BBa_B0034 range2476109 1 44 55 annotation2476125 1 BBa_B0053 range2476125 1 12396 12467 annotation2476107 1 BBa_K823008 range2476107 1 1 35 annotation2476118 1 BBa_K1650034 range2476118 1 952 963 annotation2476128 1 BBa_B0054 range2476128 1 12476 12544 annotation2476113 1 BBa_B0010 range2476113 1 776 855 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_B0054_sequence 1 attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K1650034_sequence 1 aaggaggaatta BBa_K1650009_sequence 1 tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaatcataaaaaatttatctgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacactactagagaaggaggaattatactagatgcggcaccggcaggataacttactggcaaacagaacattgttgcccggtatggcttccggtcagtacgtattcaggctctgcactttctctccggtggtacgctatttttccctcctcccctgcctttgtattctttcgttttcgtctccggcagccatgctgtctccgggtgaccgcagtgcaattcagcagcaacagcagcagttgctggatgaaaaccagcgtcagcgtgatgcgctggagcgcagtgcgccgctgaccatcacgccgtctccggaaacgtctgccggtactgaaggtccctgctttacggtgtcaagcattgttgtcagtggggccacccgactgacgtctgcagaaaccgacagactggtggcaccgtgggtgaatcagtgtctgaatatcacggggctgaccgcggtcacggatgccatgacggacagctatatacgccggggatatatcaccagccgggcctttctgacagagcaggacctttcagggggcgtactgcacataacggtcatggaaggcaggctgcagcaaatccgggcggaaggcgctgaccttcctgcccgcaccctgaagatggttttcccgggaatggaggggaaggttctgaacctgcgggatattgagcaggggatggagcagattaatcgtctgcgtacggagccggtacagattgaaatatcgcccggtgaccgtgagggatggtcggtggtgacactgacggcattgccggaatggcctgtcacagggagcgtgggcatcgacaacagcgggcagaagagtaccggtacggggcagttaaatggtgtcctttcctttaataatcctctggggctggctgacaactggtttgtcagcgggggacggagcagtgacttttcggtgtcacatgatgcgaggaattttgccgccggtgtcagtctgccgtatggctataccctggtggattacacgtattcatggagtgactacctcagcaccattgataaccggggctggcggtggcgttccacgggagacctgcagactcaccggctgggactgtcgcatgtcctgttccgtaacggggacatgaagacagcactgaccggaggtctgcagcaccgcattattcacaattatctggatgatgttctgcttcagggcagcagccgtaaactcacttcattttctgtcgggctgaatcacacccacaagtttctggggggggtcggaacactgaatccggtattcacacgggggatgccctggttcggcgcagaaagcgaccacgggaaaaggggagacctgcccgtaaatcagttccggaaatggtcggtgagtgccagttttcagcgccccgtcacggacagggtgtggtggctgaccagcgcttatgcccagtggtcaccggaccgtcttcatggtgtggaacaactgagcctcgggggtgagagttcagtgcgtggctttaaggatcagtatatctccggtaataacggcggttatctgcggaatgagctgtcctggtctctgttctccctgccatatgtgggaactgtccgtgcagtggctgcactggacggcggctggctgcactctgacagcgatgacccgtactcgtccggcacgctgtggggtgctgctgccgggctcagcaccaccagtggccatgtttccggttcgttcactgccggactgcctctggtttacccggactggcttgcccctgaccatctcacggtttactggcgcgttgccgtcgcgttttaagggattattaccatgcatcagcctcccgttcgcttcacttaccgcctgctgagttacctggtcagtgcgattatcgccgggcagccgttgttaccggctgtgggggccgtcatcaccccacaaaacggggccggaatggataaagcggcaaatggtgtgccggtcgtgaacattgccacgccgaacggggccgggatttcgcataaccggtttacggattacaacgtcgggaaggaagggctgattctcaataatgccaccggtaagcttaatccgacgcagcttggtggactgatacagaataacccgaacctgaaagcgggcggggaagcgaagggtatcatcaacgaagtgaccggcggtaagcgttcactgctgcagggctatacggaagtggccggcaaagcggcgaatgtgatggttgccaacccgtatggtatcacctgtgacggctgtggctttatcaacacgccgcacgcgacgctcaccacgggcaaacctgtgatgaatgccgacggcagcctgcaggcgctggaggtgactgaaggcagtatcaccatcaatggcgcgggcctggacggcacccggagcgatgccgtatccattattgcccgtgcaacggaagtgaatgccgcgcttcatgcgaaggatttaactgtcactgcaggcgctaaccggataactgcagatggtcgcgtcagtgccctgaagggcgaaggtaatgtgccgaaagttgccgttgataccggcgctctcggtggaatgtacgccaggcgtattcatctgacctccactgaaagtggtgtcggggttaatcttggtaacctttatgcccgcgagggcgatatcatactgagcagttccggaaaactggtcctgaagaacagccttgccggcggcaataccaccgtaaccggaacggatgtctcactttcaggggataacaaagccggaggaaatctcagcgttaccgggacaacgggactgacactgaatcagtcccgtctggtgacggataaaaatctggtgctgtcttcatccgggcagattgtacagaacggtggtgaactgactgccgggcagaacgccatgctcagtgcacagcacctgaaccagacttccgggaccgtgaatgcagctgaaaatgtcaccttgaccaccaccgatgataccacactgaaaggccgcagcgttgccgggaaaacactcaccgtcagttccggcagcctgaacaacggtgggacactggttgccgggcgcgatgccacggtgaaaaccgggacattcagtaataccggtaccgtccaggggaatggcctgaaagttaccgccactgacctgaccagcaccggcagtattaaaagtggcagcacactcgatatcagcgcccgcaatgccacactgtccggtgatgccggagcaaaagacagagccctcgttaccgtcagcggtacacttgaaaaccgcggcagactcgtcagcgatgacgtgctgacgctcagtgccacgcagataaacaacagcggtaccctctccggggcaaaggaacttgtggcttctgcagacacactgaccaccacagaaaaatcggtcacaaacagtgacggtaacctcatgctggacagcgcgtcttccacactggcgggtgaaaccagtgcgggtggcacggtgtctgtaaaaggcaacagtctgaagaccacgaccactgcgcagacgcagggcaacagtgtcagcgtggatgtgcagaacgcacagcttgacggaacacaggctgccagagacatccttaccctgaacgccagtgaaaagctcacccacagcgggaaaagcagtgccccgtcgctcagcctcagtgcgccggaactgaccagcagcggcgtacttgttggttccgccctgaatacacagtcacagaccctgaccaacagcggtctgttgcaggggaaggcctcactcaccgttaacacacagaggcttgataatcagcagaacggcacgctgtacagtgctgcagacctgacgctggatataccggacatccgcaacagcgggcttatcaccggtgataatggtttaatgttaaatgctgtctccctcagcaatccgggaaaaatcatcgctgacacgctgagcgtcagggcgaccacgctggatggtgacggcctgttgcagggcgccggtgcactggcgcttgcaggtgacacactctcactgggtagtaacggacgctggctgacggcgggcgacctctccctccggggtaa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tgctggctgccgccgctgacacccgacagacaaccgggaagaacagcagcaaaggtggcggcgtgggcgtcagctttggtggtggaaccaatggcggaggcctcagtatcttcgccggtatcaatggttcagagggcagggagaaaggcaacggcacgacctggaccgaaaccacgctggatgcgggtaaaaatgtctccctcacaagcggacgtgataccaccctgtcgggggctcaggtcagtggcgagaaagtcacagcagatgtcggcaacaacctgaccatttcgagccttcaggacagtgaccgttacgacagcagacagaaccgcgtggccgcaggtggcagcttcacctttggctcgatgtccggcagcggctatgccagcatcagccaggacaaaattaaaagcaattacgattctgtccgggaacagagtggtatctacgccggaaaagacgggtttgatgttactgtggggaatcacactcagctcaatggtgcggtaattgcgtccacggcaacggatgacaaaaacagcctcaataccaacacgctgggctggtcggatatccataaccaggcagactataaagccagccataccggaatcagtttatcgggtggctcagggatgtcagccagccagatggtggcctcaaatgcaatagccggtgcggcgaacgcacttaccggtatgtccggcagcagtggacatgctgaaggtaccacgtcatctgccatcagcggcggaaatctcatcatccgtaataaggagagtcagaagcaggatattgccgggctcagccgggacccggaaaacgccaacggcagcattgcaccgatatttgacagggagaaagagcagaagcgtcttcaggaagcgcaggttatcagccagattagtggtcagatgagtaacatcgtcatgacgtatggcgaaactgaagcgatgaaagcagccaggaaagaacatcctggcatgagcgatgcacagttgcgggagaccccggaatacagagaggtcatgaaaggctacggtacgggcagtacgccgcagatggtggtacaggccatcacgggcgtgctgggcggactgaatgcagggaatccggggcaggtactggctggcggtctgaatccggcagtggcacagcttataaaacaggctacaggcgataaccgggaagcaaacctgatggctcatgcagtctggggagcacttgccgcacaactgggcggaaacaatgcggcttccggtgctgccggggcattcagtggtgaacttgcagcccggtatatcattgataactactacggtggtcgtaccgataacctgagtgaacaggaacgtcagcaaatcagcatgctggccaccattgcttcaggtattgcaggcgggcttgtcggcaacagtacaagtgctgccggtacgggggcacaggcaggtaggaactcggttgagaataatgcaatgtctggcctggaagggtttgggaccggcttccagagttatgttcaggcccaggaggcgctggtaaataataccaatcttactgataaaaatggcaaagtactgaatccggcaacaccagaggaaattaagtacgcctctgataagctggtaaccggttctatacctgaaggacaggaccctgccagaggtttactgatttcatggggagccggagcatctgtttttgggggagaacttatagctccagcagttggcacggttgctgttatcggaggtaccctcttaggtggtactacagatgctgttaagcagtttttgaccctgaaaccgggtgagcaatacagtactacagatactcttattgccgcaggtgaaggtgggcttactcaggggaaaggggtcatcttcagtacatttattaacacaatgggggcttacttaggaagtaaagctaaaggggaagaccctacagggccaatggtgggaaatgctataggtactgcactgggaaataaagccggagataaattcacaaaagaaatgctctcaagaggctttggctctgtcacatcagaggttacaggtacagttactggtagtgttattgggacagtgactgattatcagatcgaaaagctaggaaaaggtaataaggaaggggcaaaatgaatgaagaagaaactatttgcccttttaaaatatattattttttttcctatgctatgtactgtacttggtcttttaggtatacctataggtttaattgtgaattttttgagaacgggctcttttgacttcaacttaaaagatgaaattgatgttgttttattcactttaaaaataggaatccccataggctttattcttgggctgggtttgtggggccttagtatcttagacagaaaatagtactagagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttactagagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg BBa_K823008_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_B0053_sequence 1 tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt BBa_K1650013_sequence 1 atgcggcaccggcaggataacttactggcaaacagaacattgttgcccggtatggcttccggtcagtacgtattcaggctctgcactttctctccggtggtacgctatttttccctcctcccctgcctttgtattctttcgttttcgtctccggcagccatgctgtctccgggtgaccgcagtgcaattcagcagcaacagcagcagttgctggatgaaaaccagcgtcagcgtgatgcgctggagcgcagtgcgccgctgaccatcacgccgtctccggaaacgtctgccggtactgaaggtccctgctttacggtgtcaagcattgttgtcagtggggccacccgactgacgtctgcagaaaccgacagactggtggcaccgtgggtgaatcagtgtctgaatatcacggggctgaccgcggtcacggatgccatgacggacagctatatacgccggggatatatcaccagccgggcctttctgacagagcaggacctttcagggggcgtactgcacataacggtcatggaaggcaggctgcagcaaatccgggcggaaggcgctgaccttcctgcccgcaccctgaagatggttttcccgggaatggaggggaaggttctgaacctgcgggatattgagcaggggatggagcagattaatcgtctgcgtacggagccggtacagattgaaatatcgcccggtgaccgtgagggatggtcggtggtgacactgacggcattgccggaatggcctgtcacagggagcgtgggcatcgacaacagcgggcagaagagtaccggtacggggcagttaaatggtgtcctttcctttaataatcctctggggctggctgacaactggtttgtcagcgggggacggagcagtgacttttcggtgtcacatgatgcgaggaattttgccgccggtgtcagtctgccgtatggctataccctggtggattacacgtattcatggagtgactacctcagcaccattgataaccggggctggcggtggcgttccacgggagacctgcagactcaccggctgggactgtcgcatgtcctgttccgtaacggggacatgaagacagcactgaccggaggtctgcagcaccgcattattcacaattatctggatgatgttctgcttcagggcagcagccgtaaactcacttcattttctgtcgggctgaatcacacccacaagtttctggggggggtcggaacactgaatccggtattcacacgggggatgccctggttcggcgcagaaagcgaccacgggaaaaggggagacctgcccgtaaatcagttccggaaatggtcggtgagtgccagttttcagcgccccgtcacggacagggtgtggtggctgaccagcgcttatgcccagtggtcaccggaccgtcttcatggtgtggaacaactgagcctcgggggtgagagttcagtgcgtggctttaaggatcagtatatctccggtaataacggcggttatctgcggaatgagctgtcctggtctctgttctccctgccatatgtgggaactgtccgtgcagtggctgcactggacggcggctggctgcactctgacagcgatgacccgtactcgtccggcacgctgtggggtgctgctgccgggctcagcaccaccagtggccatgtttccggttcgttcactgccggactgcctctggtttacccggactggcttgcccctgaccatctcacggtttactggcgcgttgccgtcgcgttttaagggattattaccatgcatcagcctcccgttcgcttcacttaccgcctgctgagttacctggtcagtgcgattatcgccgggcagccgttgttaccggctgtgggggccgtcatcaccccacaaaacggggccggaatggataaagcggcaaatggtgtgccggtcgtgaacattgccacgccgaacggggccgggatttcgcataaccggtttacggattacaacgtcgggaaggaagggctgattctcaataatgccaccggtaagcttaatccgacgcagcttggtggactgatacagaataacccgaacctgaaagcgggcggggaagcgaagggtatcatcaacgaagtgaccggcggtaagcgttcactgctgcagggctatacggaagtggccggcaaagcggcgaatgtgatggttgccaacccgtatggtatcacctgtgacggctgtggctttatcaacacgccgcacgcgacgctcaccacgggcaaacctgtgatgaatgccgacggcagcctgcaggcgctggaggtgactgaaggcagtatcaccatcaatggcgcgggcctggacggcacccggagcgatgccgtatccattattgcccgtgcaacggaagtgaatgccgcgcttcatgcgaaggatttaactgtcactgcaggcgctaaccggataactgcagatggtcgcgtcagtgccctgaagggcgaaggtaatgtgccgaaagttgccgttgataccggcgctctcggtggaatgtacgccaggcgtattcatctgacctccactgaaagtggtgtcggggttaatcttggtaacctttatgcccgcgagggcgatatcatactgagcagttccggaaaactggtcctgaagaacagccttgccggcggcaataccaccgtaaccggaacggatgtctcactttcaggggataacaaagccggaggaaatctcagcgttaccgggacaacgggactgacactgaatcagtcccgtctggtgacggataaaaatctggtgctgtcttcatccgggcagattgtacagaacggtggtgaactgactgccgggcagaacgccatgctcagtgcacagcacctgaaccagacttccgggaccgtgaatgcagctgaaaatgtcaccttgaccaccaccgatgataccacactgaaaggccgcagcgttgccgggaaaacactcaccgtcagttccggcagcctgaacaacggtgggacactggttgccgggcgcgatgccacggtgaaaaccgggacattcagtaataccggtaccgtccaggggaatggcctgaaagttaccgccactgacctgaccagcaccggcagtattaaaagtggcagcacactcgatatcagcgcccgcaatgccacactgtccggtgatgccggagcaaaagacagagccctcgttaccgtcagcggtacacttgaaaaccgcggcagactcgtcagcgatgacgtgctgacgctcagtgccacgcagataaacaacagcggtaccctctccggggcaaaggaacttgtggcttctgcagacacactgaccaccacagaaaaatcggtcacaaacagtgacggtaacctcatgctggacagcgcgtcttccacactggcgggtgaaaccagtgcgggtggcacggtgtctgtaaaaggcaacagtctgaagaccacgaccactgcgcagacgcagggcaacagtgtcagcgtggatgtgcagaacgcacagcttgacggaacacaggctgccagagacatccttaccctgaacgccagtgaaaagctcacccacagcgggaaaagcagtgccccgtcgctcagcctcagtgcgccggaactgaccagcagcggcgtacttgttggttccgccctgaatacacagtcacagaccctgaccaacagcggtctgttgcaggggaaggcctcactcaccgttaacacacagaggcttgataatcagcagaacggcacgctgtacagtgctgcagacctgacgctggatataccggacatccgcaacagcgggcttatcaccggtgataatggtttaatgttaaatgctgtctccctcagcaatccgggaaaaatcatcgctgacacgctgagcgtcagggcgaccacgctggatggtgacggcctgttgcagggcgccggtgcactggcgcttgcaggtgacacactctcactgggtagtaacggacgctggctgacggcgggcgacctctccctccggggtaaaacactgcataccgcagggaccacgcagggacagaatctcaccgtgcaggcggacagatgggcgaacagtggttccgtgcaggcaaccggtaaccttactgcttcggcaaccggtcagttgaccagtaccggcgatatcatgagccagggtgacaccacgctgaacgcagccaccacggacaaccggggcagtctgctttcggccggcacgctctcccttgatggaaactcactggataacagcggcactgtccagggtaaccatgtcacgattcgccagaacggtgtcaccaacagtggcacgctcaccgggatcgccgcgctgacgcttgccgcccgtatggatatggcatcccctcaacctgcgctgatgaataacggaggttcattgctgaccagcggcgatctgacaatcaccgcaggcagtctggcaaacagcggggcgatccaggcggctgacagcctgactgcacgtctgacgggtgagctcgtcagcacagcaggcagcaaagtcacctcgaacggtgaaatggcgctcagtgcactgaatttaagcaacagcggacaatggattgcaaaaaatctgactctgaaggcgaactcactgaccagtgcgggtgacatcaccggtgtggatgctctcacgctcacggtgaatcagacgctgaacaatcacgcgagcggaaaactgctcagtgcaggtgtgctgacgctgaaggcagacagtgtcaaaaacgacgggcaattacagggaaatgccaccaccatcacggcaggacaactcacaaacggcgggcatctgcagggcgaaacgctgacgctggccgcctccggtggcgtgaacaaccgttccggtggtgttctgatgagccggaatgcactgaatgtcagtactgcgaccctgagtaaccagggcacgatacagggtggtggcggggtttccctgaacgccactgaccgtctgcagaacgacggcaaaatcctctccggcagtaacctgacgctgacggcgcaggtgctggcgaacaccggcagcggactggtacaggctgccaccctgctgctggatgtggtgaatactgtcaacggcggacgcgtacttgccaccggcagtgccgacgttaaaggaaccacgctgaataataccggtacgtttcagggggcggacctgctggtgaattaccacacattcagcaacagcggtaccctgctgggaacctccgggcttggcgtcaagggcagttcactgctgcaaaatggtacagggcggctgtacagtgcaggcaacctgctgcttgacgctcaggacttcagtggtcagggccaggtggtggccaccggtgatgtcacactgaaactgattgctgccctcacgaatcatggtaccctggccgcagggaaaaccctttccgtcacgtcgcagaacgccgtcaccaacggcggtgtgatgcagggtgatgccatggtgctcggtgccggagaggcattcaccaacaatggaacgctgactgccggtaaaggcaacagtgttttcagcgcacagcgtcttttccttaacgcaccgggttcacttcaggccggtggcgatgtgagtctgaacagccggagtgatatcaccatcagtggttttaccggcacggcaggcagtctgacaatgaatgtggccggtaccctgctgaacagtgcgctgatttatgcggggaataacctgaagctgtttacagaccgtctgcataaccagcatggtgatatcctggccggcaacagtctgtgggtacagaaggacagcagcggtacggcaaacagcgaaatcattaaccgttccggtaatattgaaaccacacgtggcgatatcaccatgaatacggcgcatctgctgaattcatgggatgccatttcggccagccatgaggtcattcctggaagcagtcacggggtgatttcaccggttccggaaaataaccggtggtggggtgttgtccggcatgatggtgtggaatatctggccgtgtactggggtaagggtgccacggtacctgatgaataccgtatccgtacgggagatacggaaacggtgacagtcagtgcctccggccatgctgccaggatatccggcggcgctgatatgcatatccgtgcaggacgactggataatgaggcatcgtttattctcgccggtggcggtatgaccctgagtggcgacacactgaacaatcagggctggcaggaggggaccaccggaaaggaaacggtctggcggcttgcctcaggctcactccccaaagcatggtttactgagccatggtataaggtctatcgccaggtctcaccggatgcgacagaagccagtggcacctctccggcaggtcagtaccgtgcggttatcagcgctgccggtgatgtatctgccagctttgccactgacaccggtaataccactgtcatgccccgggcaggtggtgcaggtaatacgataacggtgccttcgctgaactccctgacaccaccgacggtcagtcagggggtgagcggtgaagccctgctgaatgaaagcggtaccgggataaccggccctgtatggaatgacgcgctgccggatacgctgaaggatatcccgggtgcgctgtcgctgtccggggcaagtgtaagcagctatcccctgccatccggtaacaatggttattttgtcccgtccacggacccggacagtccgtatctgattacggtgaacccgaaactggatggtctcggaaaggtggacagcagtctgtttgccggactgtatgacctgctcaggatgcaacccggagaggcaccacgtgaaacagacccggcatataccgatgaaaaacagtttctgggctcgtcgtacattcttgaccgtcttggcctgaaaccggaaaaagattaccgctttctgggggatgcggcttttgatacccggtatgtcagtaacgtcatactgaaccagacgggctcacgttatatcaacggcacaggttctgacctggcgcagatgaaatacctgatggacagcgcggcagcacaacagaaagcgctcggactgacctttggtgtgtcgctgaccgccgggcaggttgcacaactgaccagaagcctgctgtggtgggagtccgtcaccatcaacggacagacagtcatggttccgaaactgtatctgtctccggaagatatcacccttcataatggcagcgttatcagcgggaacaacgtgcagcttgcgggtggcaatatcaccaacagcggcagcagcatcaacgcacagaatgacctcctgctcgacagaacaggtagtatcgacaacctgaatgccggactgataaacgccggtggtgcactgaacctgaaggccatcggggatatcggcaatatcagctcagtcatcagcggtaaaaccgtatccctggaaagcgccaccggaaacatcagtaatctcacccggacagaacagtgggcgatgaacaacggatataaccatttcagcggaacggataccggtccgctggctgctgtcagggcaacggactcactgtttatgggcgcagccggagatatcagtatcaccggtgccgccgtttctgccggtgacagcgtgttactggccgccggtaatgacctgaacatgaacgcgatacaggccggagaacgtcgtcgctatggcgggtccgggtggtatgaaactcatgccgtggcccccacggtcactgccgggaacagtctgatgctttccgccggtcgtgatgtgaacagccaggctgccggtattacagctgaaaacagtatggatatccgcgccggacgggatgtgaacatggctgcggagtccacaggcgcaggtgaccacgacagcactttcagcatgaaaacggttcatgattcggtccggcagcagggaacagacatgaccagcggcggtgacatcacggttactgccggacgggatatcacctcagttgcgactgctgtaacggcaaagggcgatatccgtgtgaatgccggtcatgacatcgtactgggtactgccacggaaagtgattatcactacagtgaatcaggggaaacccgtaaccgtctcctcagccatcagaccacccggaccatcacggaagacagcgtcacccgtgagaagggctccctgctgtcgggaaaccgcgtgaccgttaacgccggtaataacctgacggtacagggttcggatgtggtggctgaccgggatgtgtcactggcggcggataaccatgttgatgttcttgctgccaccagtacagatacgtcctggcgctttaaggaaacgaagacatccggtctgacgggtaccggcggtattggtttcaccactggcagcagtaagacaacgcacgaccgccgcgaggccgggacaacgcagagtcagagtgccagtaccatcggctccactgccggtaatgtcagtattaccgcgggcaaacaggctcatatcagcggttcggatgtgattgcgaaccgggatatcagcattaccggtgacagtgtggtggttgacccggggcatgaccgtcgtactgtggacgaaaaatttgagcagaagaaaagcgggctgacggttgccctgtccggtgcggtgggtagtgccatcaataatgcggtcaccatggcccgggaggcgaaagaaaccagcgacagccgtctggcggcactgaaaggaacacaggcggttctgtctggtgtacaggccggtgttaaccacggactgcagcaacagagtgctgacccgaataatggtatcggcgtgagcatttcgctcaaccatcagcagtcgaagtctgagacgaaatatcagcacgatatcgtctccggctcaaccctcagcgccggtaacaatgtctctgtcactgccacggggaaaaataaggaccataacaacagcggcgatatgctcattaccgggagccagattaagtccggcaatgacaccagcctgaatgcacagaacgatattctgctggctgccgccgctgacacccgacagacaaccgggaagaacagcagcaaaggtggcggcgtgggcgtcagctttggtggtggaaccaatggcggaggcctcagtatcttcgccggtatcaatggttcagagggcagggagaaaggcaacggcacgacctggaccgaaaccacgctggatgcgggtaaaaatgtctccctcacaagcggacgtgataccaccctgtcgggggctcaggtcagtggcgagaaagtcacagcagatgtcggcaacaacctgaccatttcgagccttcaggacagtgaccgttacgacagcagacagaaccgcgtggccgcaggtggcagcttcacctttggctcgatgtccggcagcggctatgccagcatcagccaggacaaaattaaaagcaattacgattctgtccgggaacagagtggtatctacgccggaaaagacgggtttgatgttactgtggggaatcacactcagctcaatggtgcggtaattgcgtccacggcaacggatgacaaaaacagcctcaataccaacacgctgggctggtcggatatccataaccaggcagactataaagccagccataccggaatcagtttatcgggtggctcagggatgtcagccagccagatggtggcctcaaatgcaatagccggtgcggcgaacgcacttaccggtatgtccggcagcagtggacatgctgaaggtaccacgtcatctgccatcagcggcggaaatctcatcatccgtaataaggagagtcagaagcaggatattgccgggctcagccgggacccggaaaacgccaacggcagcattgcaccgatatttgacagggagaaagagcagaagcgtcttcaggaagcgcaggttatcagccagattagtggtcagatgagtaacatcgtcatgacgtatggcgaaactgaagcgatgaaagcagccaggaaagaacatcctggcatgagcgatgcacagttgcgggagaccccggaatacagagaggtcatgaaaggctacggtacgggcagtacgccgcagatggtggtacaggccatcacgggcgtgctgggcggactgaatgcagggaatccggggcaggtactggctggcggtctgaatccggcagtggcacagcttataaaacaggctacaggcgataaccgggaagcaaacctgatggctcatgcagtctggggagcacttgccgcacaactgggcggaaacaatgcggcttccggtgctgccggggcattcagtggtgaacttgcagcccggtatatcattgataactactacggtggtcgtaccgataacctgagtgaacaggaacgtcagcaaatcagcatgctggccaccattgcttcaggtattgcaggcgggcttgtcggcaacagtacaagtgctgccggtacgggggcacaggcaggtaggaactcggttgagaataatgcaatgtctggcctggaagggtttgggaccggcttccagagttatgttcaggcccaggaggcgctggtaaataataccaatcttactgataaaaatggcaaagtactgaatccggcaacaccagaggaaattaagtacgcctctgataagctggtaaccggttctatacctgaaggacaggaccctgccagaggtttactgatttcatggggagccggagcatctgtttttgggggagaacttatagctccagcagttggcacggttgctgttatcggaggtaccctcttaggtggtactacagatgctgttaagcagtttttgaccctgaaaccgggtgagcaatacagtactacagatactcttattgccgcaggtgaaggtgggcttactcaggggaaaggggtcatcttcagtacatttattaacacaatgggggcttacttaggaagtaaagctaaaggggaagaccctacagggccaatggtgggaaatgctataggtactgcactgggaaataaagccggagataaattcacaaaagaaatgctctcaagaggctttggctctgtcacatcagaggttacaggtacagttactggtagtgttattgggacagtgactgattatcagatcgaaaagctaggaaaaggtaataaggaaggggcaaaatgaatgaagaagaaactatttgcccttttaaaatatattattttttttcctatgctatgtactgtacttggtcttttaggtatacctataggtttaattgtgaattttttgagaacgggctcttttgacttcaacttaaaagatgaaattgatgttgttttattcactttaaaaataggaatccccataggctttattcttgggctgggtttgtggggccttagtatcttagacagaaaatag BBa_K1650033_sequence 1 aaatcataaaaaatttatctgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z