##gff-version 3
##sequence-region BBa_K1650009 1 12544
BBa_K1650009	.	ribosome_entry_site	952	963	.	+	0	ID=BBa_K1650034;Name=BBa_K1650034
BBa_K1650009	.	ribosome_entry_site	952	963	.	+	0	ID=annotation2474905;Name=BBa_K1650034;Parent=BBa_K1650034
BBa_K1650009	.	terminator	776	855	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1650009	.	engineered_region	776	855	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1650009	.	stem_loop	787	830	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1650009	.	CDS	62	767	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1650009	.	CDS	62	736	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1650009	.	engineered_tag	743	767	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1650009	.	promoter	1	35	.	+	0	ID=BBa_K823008;Name=BBa_K823008
BBa_K1650009	.	promoter	1	35	.	+	0	ID=annotation2182530;Name=J23106;Parent=BBa_K823008
BBa_K1650009	.	non_covalent_binding_site	24	29	.	+	0	ID=annotation2182529;Name=-10 box;Parent=BBa_K823008
BBa_K1650009	.	non_covalent_binding_site	1	6	.	+	0	ID=annotation2182528;Name=-35 box;Parent=BBa_K823008
BBa_K1650009	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1650009	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1650009	.	terminator	12396	12467	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_K1650009	.	stem_loop	12404	12438	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_K1650009	.	CDS	970	12387	.	+	0	ID=BBa_K1650013;Name=BBa_K1650013
BBa_K1650009	.	CDS	970	2736	.	+	0	ID=annotation2473449;Name=CdiB;Parent=BBa_K1650013
BBa_K1650009	.	CDS	12145	12385	.	+	0	ID=annotation2473451;Name=CdiI;Parent=BBa_K1650013
BBa_K1650009	.	CDS	2737	2748	.	+	0	ID=annotation2473450;Name=intergenic region;Parent=BBa_K1650013
BBa_K1650009	.	CDS	2749	12147	.	+	0	ID=annotation2473452;Name=CdiA;Parent=BBa_K1650013
BBa_K1650009	.	terminator	12476	12544	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_K1650009	.	sequence_feature	12479	12518	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_K1650009	.	stem_loop	12486	12517	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_K1650009	.	promoter	864	943	.	+	0	ID=BBa_K1650033;Name=BBa_K1650033
BBa_K1650009	.	promoter	864	943	.	+	0	ID=annotation2474906;Name=BBa_K1650033;Parent=BBa_K1650033
>BBa_K1650009
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggcttcctccgaagac
gttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaag
gtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggga
catcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactg
tccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcct
ccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaa
aaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgt
ctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgg
gtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgc
tgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaatca
taaaaaatttatctgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacactacta
gagaaggaggaattatactagatgcggcaccggcaggataacttactggcaaacagaacattgttgcccggtatggctt
ccggtcagtacgtattcaggctctgcactttctctccggtggtacgctatttttccctcctcccctgcctttgtattct
ttcgttttcgtctccggcagccatgctgtctccgggtgaccgcagtgcaattcagcagcaacagcagcagttgctggat
gaaaaccagcgtcagcgtgatgcgctggagcgcagtgcgccgctgaccatcacgccgtctccggaaacgtctgccggta
ctgaaggtccctgctttacggtgtcaagcattgttgtcagtggggccacccgactgacgtctgcagaaaccgacagact
ggtggcaccgtgggtgaatcagtgtctgaatatcacggggctgaccgcggtcacggatgccatgacggacagctatata
cgccggggatatatcaccagccgggcctttctgacagagcaggacctttcagggggcgtactgcacataacggtcatgg
aaggcaggctgcagcaaatccgggcggaaggcgctgaccttcctgcccgcaccctgaagatggttttcccgggaatgga
ggggaaggttctgaacctgcgggatattgagcaggggatggagcagattaatcgtctgcgtacggagccggtacagatt
gaaatatcgcccggtgaccgtgagggatggtcggtggtgacactgacggcattgccggaatggcctgtcacagggagcg
tgggcatcgacaacagcgggcagaagagtaccggtacggggcagttaaatggtgtcctttcctttaataatcctctggg
gctggctgacaactggtttgtcagcgggggacggagcagtgacttttcggtgtcacatgatgcgaggaattttgccgcc
ggtgtcagtctgccgtatggctataccctggtggattacacgtattcatggagtgactacctcagcaccattgataacc
ggggctggcggtggcgttccacgggagacctgcagactcaccggctgggactgtcgcatgtcctgttccgtaacgggga
catgaagacagcactgaccggaggtctgcagcaccgcattattcacaattatctggatgatgttctgcttcagggcagc
agccgtaaactcacttcattttctgtcgggctgaatcacacccacaagtttctggggggggtcggaacactgaatccgg
tattcacacgggggatgccctggttcggcgcagaaagcgaccacgggaaaaggggagacctgcccgtaaatcagttccg
gaaatggtcggtgagtgccagttttcagcgccccgtcacggacagggtgtggtggctgaccagcgcttatgcccagtgg
tcaccggaccgtcttcatggtgtggaacaactgagcctcgggggtgagagttcagtgcgtggctttaaggatcagtata
tctccggtaataacggcggttatctgcggaatgagctgtcctggtctctgttctccctgccatatgtgggaactgtccg
tgcagtggctgcactggacggcggctggctgcactctgacagcgatgacccgtactcgtccggcacgctgtggggtgct
gctgccgggctcagcaccaccagtggccatgtttccggttcgttcactgccggactgcctctggtttacccggactggc
ttgcccctgaccatctcacggtttactggcgcgttgccgtcgcgttttaagggattattaccatgcatcagcctcccgt
tcgcttcacttaccgcctgctgagttacctggtcagtgcgattatcgccgggcagccgttgttaccggctgtgggggcc
gtcatcaccccacaaaacggggccggaatggataaagcggcaaatggtgtgccggtcgtgaacattgccacgccgaacg
gggccgggatttcgcataaccggtttacggattacaacgtcgggaaggaagggctgattctcaataatgccaccggtaa
gcttaatccgacgcagcttggtggactgatacagaataacccgaacctgaaagcgggcggggaagcgaagggtatcatc
aacgaagtgaccggcggtaagcgttcactgctgcagggctatacggaagtggccggcaaagcggcgaatgtgatggttg
ccaacccgtatggtatcacctgtgacggctgtggctttatcaacacgccgcacgcgacgctcaccacgggcaaacctgt
gatgaatgccgacggcagcctgcaggcgctggaggtgactgaaggcagtatcaccatcaatggcgcgggcctggacggc
acccggagcgatgccgtatccattattgcccgtgcaacggaagtgaatgccgcgcttcatgcgaaggatttaactgtca
ctgcaggcgctaaccggataactgcagatggtcgcgtcagtgccctgaagggcgaaggtaatgtgccgaaagttgccgt
tgataccggcgctctcggtggaatgtacgccaggcgtattcatctgacctccactgaaagtggtgtcggggttaatctt
ggtaacctttatgcccgcgagggcgatatcatactgagcagttccggaaaactggtcctgaagaacagccttgccggcg
gcaataccaccgtaaccggaacggatgtctcactttcaggggataacaaagccggaggaaatctcagcgttaccgggac
aacgggactgacactgaatcagtcccgtctggtgacggataaaaatctggtgctgtcttcatccgggcagattgtacag
aacggtggtgaactgactgccgggcagaacgccatgctcagtgcacagcacctgaaccagacttccgggaccgtgaatg
cagctgaaaatgtcaccttgaccaccaccgatgataccacactgaaaggccgcagcgttgccgggaaaacactcaccgt
cagttccggcagcctgaacaacggtgggacactggttgccgggcgcgatgccacggtgaaaaccgggacattcagtaat
accggtaccgtccaggggaatggcctgaaagttaccgccactgacctgaccagcaccggcagtattaaaagtggcagca
cactcgatatcagcgcccgcaatgccacactgtccggtgatgccggagcaaaagacagagccctcgttaccgtcagcgg
tacacttgaaaaccgcggcagactcgtcagcgatgacgtgctgacgctcagtgccacgcagataaacaacagcggtacc
ctctccggggcaaaggaacttgtggcttctgcagacacactgaccaccacagaaaaatcggtcacaaacagtgacggta
acctcatgctggacagcgcgtcttccacactggcgggtgaaaccagtgcgggtggcacggtgtctgtaaaaggcaacag
tctgaagaccacgaccactgcgcagacgcagggcaacagtgtcagcgtggatgtgcagaacgcacagcttgacggaaca
caggctgccagagacatccttaccctgaacgccagtgaaaagctcacccacagcgggaaaagcagtgccccgtcgctca
gcctcagtgcgccggaactgaccagcagcggcgtacttgttggttccgccctgaatacacagtcacagaccctgaccaa
cagcggtctgttgcaggggaaggcctcactcaccgttaacacacagaggcttgataatcagcagaacggcacgctgtac
agtgctgcagacctgacgctggatataccggacatccgcaacagcgggcttatcaccggtgataatggtttaatgttaa
atgctgtctccctcagcaatccgggaaaaatcatcgctgacacgctgagcgtcagggcgaccacgctggatggtgacgg
cctgttgcagggcgccggtgcactggcgcttgcaggtgacacactctcactgggtagtaacggacgctggctgacggcg
ggcgacctctccctccggggtaaaacactgcataccgcagggaccacgcagggacagaatctcaccgtgcaggcggaca
gatgggcgaacagtggttccgtgcaggcaaccggtaaccttactgcttcggcaaccggtcagttgaccagtaccggcga
tatcatgagccagggtgacaccacgctgaacgcagccaccacggacaaccggggcagtctgctttcggccggcacgctc
tcccttgatggaaactcactggataacagcggcactgtccagggtaaccatgtcacgattcgccagaacggtgtcacca
acagtggcacgctcaccgggatcgccgcgctgacgcttgccgcccgtatggatatggcatcccctcaacctgcgctgat
gaataacggaggttcattgctgaccagcggcgatctgacaatcaccgcaggcagtctggcaaacagcggggcgatccag
gcggctgacagcctgactgcacgtctgacgggtgagctcgtcagcacagcaggcagcaaagtcacctcgaacggtgaaa
tggcgctcagtgcactgaatttaagcaacagcggacaatggattgcaaaaaatctgactctgaaggcgaactcactgac
cagtgcgggtgacatcaccggtgtggatgctctcacgctcacggtgaatcagacgctgaacaatcacgcgagcggaaaa
ctgctcagtgcaggtgtgctgacgctgaaggcagacagtgtcaaaaacgacgggcaattacagggaaatgccaccacca
tcacggcaggacaactcacaaacggcgggcatctgcagggcgaaacgctgacgctggccgcctccggtggcgtgaacaa
ccgttccggtggtgttctgatgagccggaatgcactgaatgtcagtactgcgaccctgagtaaccagggcacgatacag
ggtggtggcggggtttccctgaacgccactgaccgtctgcagaacgacggcaaaatcctctccggcagtaacctgacgc
tgacggcgcaggtgctggcgaacaccggcagcggactggtacaggctgccaccctgctgctggatgtggtgaatactgt
caacggcggacgcgtacttgccaccggcagtgccgacgttaaaggaaccacgctgaataataccggtacgtttcagggg
gcggacctgctggtgaattaccacacattcagcaacagcggtaccctgctgggaacctccgggcttggcgtcaagggca
gttcactgctgcaaaatggtacagggcggctgtacagtgcaggcaacctgctgcttgacgctcaggacttcagtggtca
gggccaggtggtggccaccggtgatgtcacactgaaactgattgctgccctcacgaatcatggtaccctggccgcaggg
aaaaccctttccgtcacgtcgcagaacgccgtcaccaacggcggtgtgatgcagggtgatgccatggtgctcggtgccg
gagaggcattcaccaacaatggaacgctgactgccggtaaaggcaacagtgttttcagcgcacagcgtcttttccttaa
cgcaccgggttcacttcaggccggtggcgatgtgagtctgaacagccggagtgatatcaccatcagtggttttaccggc
acggcaggcagtctgacaatgaatgtggccggtaccctgctgaacagtgcgctgatttatgcggggaataacctgaagc
tgtttacagaccgtctgcataaccagcatggtgatatcctggccggcaacagtctgtgggtacagaaggacagcagcgg
tacggcaaacagcgaaatcattaaccgttccggtaatattgaaaccacacgtggcgatatcaccatgaatacggcgcat
ctgctgaattcatgggatgccatttcggccagccatgaggtcattcctggaagcagtcacggggtgatttcaccggttc
cggaaaataaccggtggtggggtgttgtccggcatgatggtgtggaatatctggccgtgtactggggtaagggtgccac
ggtacctgatgaataccgtatccgtacgggagatacggaaacggtgacagtcagtgcctccggccatgctgccaggata
tccggcggcgctgatatgcatatccgtgcaggacgactggataatgaggcatcgtttattctcgccggtggcggtatga
ccctgagtggcgacacactgaacaatcagggctggcaggaggggaccaccggaaaggaaacggtctggcggcttgcctc
aggctcactccccaaagcatggtttactgagccatggtataaggtctatcgccaggtctcaccggatgcgacagaagcc
agtggcacctctccggcaggtcagtaccgtgcggttatcagcgctgccggtgatgtatctgccagctttgccactgaca
ccggtaataccactgtcatgccccgggcaggtggtgcaggtaatacgataacggtgccttcgctgaactccctgacacc
accgacggtcagtcagggggtgagcggtgaagccctgctgaatgaaagcggtaccgggataaccggccctgtatggaat
gacgcgctgccggatacgctgaaggatatcccgggtgcgctgtcgctgtccggggcaagtgtaagcagctatcccctgc
catccggtaacaatggttattttgtcccgtccacggacccggacagtccgtatctgattacggtgaacccgaaactgga
tggtctcggaaaggtggacagcagtctgtttgccggactgtatgacctgctcaggatgcaacccggagaggcaccacgt
gaaacagacccggcatataccgatgaaaaacagtttctgggctcgtcgtacattcttgaccgtcttggcctgaaaccgg
aaaaagattaccgctttctgggggatgcggcttttgatacccggtatgtcagtaacgtcatactgaaccagacgggctc
acgttatatcaacggcacaggttctgacctggcgcagatgaaatacctgatggacagcgcggcagcacaacagaaagcg
ctcggactgacctttggtgtgtcgctgaccgccgggcaggttgcacaactgaccagaagcctgctgtggtgggagtccg
tcaccatcaacggacagacagtcatggttccgaaactgtatctgtctccggaagatatcacccttcataatggcagcgt
tatcagcgggaacaacgtgcagcttgcgggtggcaatatcaccaacagcggcagcagcatcaacgcacagaatgacctc
ctgctcgacagaacaggtagtatcgacaacctgaatgccggactgataaacgccggtggtgcactgaacctgaaggcca
tcggggatatcggcaatatcagctcagtcatcagcggtaaaaccgtatccctggaaagcgccaccggaaacatcagtaa
tctcacccggacagaacagtgggcgatgaacaacggatataaccatttcagcggaacggataccggtccgctggctgct
gtcagggcaacggactcactgtttatgggcgcagccggagatatcagtatcaccggtgccgccgtttctgccggtgaca
gcgtgttactggccgccggtaatgacctgaacatgaacgcgatacaggccggagaacgtcgtcgctatggcgggtccgg
gtggtatgaaactcatgccgtggcccccacggtcactgccgggaacagtctgatgctttccgccggtcgtgatgtgaac
agccaggctgccggtattacagctgaaaacagtatggatatccgcgccggacgggatgtgaacatggctgcggagtcca
caggcgcaggtgaccacgacagcactttcagcatgaaaacggttcatgattcggtccggcagcagggaacagacatgac
cagcggcggtgacatcacggttactgccggacgggatatcacctcagttgcgactgctgtaacggcaaagggcgatatc
cgtgtgaatgccggtcatgacatcgtactgggtactgccacggaaagtgattatcactacagtgaatcaggggaaaccc
gtaaccgtctcctcagccatcagaccacccggaccatcacggaagacagcgtcacccgtgagaagggctccctgctgtc
gggaaaccgcgtgaccgttaacgccggtaataacctgacggtacagggttcggatgtggtggctgaccgggatgtgtca
ctggcggcggataaccatgttgatgttcttgctgccaccagtacagatacgtcctggcgctttaaggaaacgaagacat
ccggtctgacgggtaccggcggtattggtttcaccactggcagcagtaagacaacgcacgaccgccgcgaggccgggac
aacgcagagtcagagtgccagtaccatcggctccactgccggtaatgtcagtattaccgcgggcaaacaggctcatatc
agcggttcggatgtgattgcgaaccgggatatcagcattaccggtgacagtgtggtggttgacccggggcatgaccgtc
gtactgtggacgaaaaatttgagcagaagaaaagcgggctgacggttgccctgtccggtgcggtgggtagtgccatcaa
taatgcggtcaccatggcccgggaggcgaaagaaaccagcgacagccgtctggcggcactgaaaggaacacaggcggtt
ctgtctggtgtacaggccggtgttaaccacggactgcagcaacagagtgctgacccgaataatggtatcggcgtgagca
tttcgctcaaccatcagcagtcgaagtctgagacgaaatatcagcacgatatcgtctccggctcaaccctcagcgccgg
taacaatgtctctgtcactgccacggggaaaaataaggaccataacaacagcggcgatatgctcattaccgggagccag
attaagtccggcaatgacaccagcctgaatgcacagaacgatattctgctggctgccgccgctgacacccgacagacaa
ccgggaagaacagcagcaaaggtggcggcgtgggcgtcagctttggtggtggaaccaatggcggaggcctcagtatctt
cgccggtatcaatggttcagagggcagggagaaaggcaacggcacgacctggaccgaaaccacgctggatgcgggtaaa
aatgtctccctcacaagcggacgtgataccaccctgtcgggggctcaggtcagtggcgagaaagtcacagcagatgtcg
gcaacaacctgaccatttcgagccttcaggacagtgaccgttacgacagcagacagaaccgcgtggccgcaggtggcag
cttcacctttggctcgatgtccggcagcggctatgccagcatcagccaggacaaaattaaaagcaattacgattctgtc
cgggaacagagtggtatctacgccggaaaagacgggtttgatgttactgtggggaatcacactcagctcaatggtgcgg
taattgcgtccacggcaacggatgacaaaaacagcctcaataccaacacgctgggctggtcggatatccataaccaggc
agactataaagccagccataccggaatcagtttatcgggtggctcagggatgtcagccagccagatggtggcctcaaat
gcaatagccggtgcggcgaacgcacttaccggtatgtccggcagcagtggacatgctgaaggtaccacgtcatctgcca
tcagcggcggaaatctcatcatccgtaataaggagagtcagaagcaggatattgccgggctcagccgggacccggaaaa
cgccaacggcagcattgcaccgatatttgacagggagaaagagcagaagcgtcttcaggaagcgcaggttatcagccag
attagtggtcagatgagtaacatcgtcatgacgtatggcgaaactgaagcgatgaaagcagccaggaaagaacatcctg
gcatgagcgatgcacagttgcgggagaccccggaatacagagaggtcatgaaaggctacggtacgggcagtacgccgca
gatggtggtacaggccatcacgggcgtgctgggcggactgaatgcagggaatccggggcaggtactggctggcggtctg
aatccggcagtggcacagcttataaaacaggctacaggcgataaccgggaagcaaacctgatggctcatgcagtctggg
gagcacttgccgcacaactgggcggaaacaatgcggcttccggtgctgccggggcattcagtggtgaacttgcagcccg
gtatatcattgataactactacggtggtcgtaccgataacctgagtgaacaggaacgtcagcaaatcagcatgctggcc
accattgcttcaggtattgcaggcgggcttgtcggcaacagtacaagtgctgccggtacgggggcacaggcaggtagga
actcggttgagaataatgcaatgtctggcctggaagggtttgggaccggcttccagagttatgttcaggcccaggaggc
gctggtaaataataccaatcttactgataaaaatggcaaagtactgaatccggcaacaccagaggaaattaagtacgcc
tctgataagctggtaaccggttctatacctgaaggacaggaccctgccagaggtttactgatttcatggggagccggag
catctgtttttgggggagaacttatagctccagcagttggcacggttgctgttatcggaggtaccctcttaggtggtac
tacagatgctgttaagcagtttttgaccctgaaaccgggtgagcaatacagtactacagatactcttattgccgcaggt
gaaggtgggcttactcaggggaaaggggtcatcttcagtacatttattaacacaatgggggcttacttaggaagtaaag
ctaaaggggaagaccctacagggccaatggtgggaaatgctataggtactgcactgggaaataaagccggagataaatt
cacaaaagaaatgctctcaagaggctttggctctgtcacatcagaggttacaggtacagttactggtagtgttattggg
acagtgactgattatcagatcgaaaagctaggaaaaggtaataaggaaggggcaaaatgaatgaagaagaaactatttg
cccttttaaaatatattattttttttcctatgctatgtactgtacttggtcttttaggtatacctataggtttaattgt
gaattttttgagaacgggctcttttgacttcaacttaaaagatgaaattgatgttgttttattcactttaaaaatagga
atccccataggctttattcttgggctgggtttgtggggccttagtatcttagacagaaaatagtactagagtccggcaa
aaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttactagagattagca
gaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg