BBa_K1650009
type: ComponentDefinition
persistent identity: BBa K1650009
display id: BBa_K1650009
version: 1
was generated by: iGEM to SBOL conversion
description: Composite carrying the Lac-inducible CDI-operon,constitutively expressed RFP
created: 2015-09-17T11:00:00Z
modified: 2015-09-19T12:10:04Z
mutable provenance: The CDI-operon was PCR amplified from genomic DNA of E.coli strain EC93. Other parts were taken from the registry.
owned by: james
owned by: myers
mutable description: Note: This part is similar to BBa_K1650007 except that it does not contain lacI and RFP expression is driven by a constitutive promoter instead of a lac-inducible promoter. The composite part contains a lac-inducible CDI operon which gives your strain the capability to inhibit growth of bacterial cells that express BamA in their outer membrane (most gram negative bacteria). Additionally the part contains mRFP1 (BBa_E1010) driven by constitutive promoter. IPTG-induced cells harboring BBa_K1650009 express CdiB which forms a beta-barrel structure. The CdiA protein is transported through CdiB and is displayed on the cell surface. When getting in contact with BamA of a target cell, the C-terminal domain is delivered into the target cell acting as pore forming protein thus destroying the proton motoric force of the target cell. BBa_K1650009 could be used to improve transformation efficiency. Induced CDI+ cells would kill false positive cells
discontinued: false
dominant: false
group u list: _2067_
m user id: 27248
owner id: 27248
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: BBa_K1650009 was toxic for cells in pSB1C3 backbone, presumably because of the high metabolic burden. In accordance with iGEM Headquarter, we submitted BBa_K1650009 in the low copy pSB4C5 backbone
star: false
creator: Daniel Stukenberg
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K823008
component: BBa_E1010
component: BBa_K1650033
component: BBa_B0034
component: BBa_B0010
component: BBa_K1650013
component: BBa_K1650034
component: BBa_B0054
component: BBa_B0053
sequence annotation: BBa_K823008
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_K1650033
sequence annotation: BBa_K1650034
sequence annotation: BBa_K1650013
sequence annotation: BBa_B0053
sequence annotation: BBa_B0054
sequence: https://synbiohub.org/public/igem/BBa_K1650009_sequence/1

BBa_K823008
type: Component
persistent identity: component2476107
display id: component2476107
version: 1
top level: BBa_K1650009
definition: BBa_K823008
access: public

BBa_E1010
type: Component
persistent identity: component2476112
display id: component2476112
version: 1
top level: BBa_K1650009
definition: mRFP1
access: public

BBa_K1650033
type: Component
persistent identity: component2476116
display id: component2476116
version: 1
top level: BBa_K1650009
definition: BBa_K1650033
access: public

BBa_B0034
type: Component
persistent identity: component2476109
display id: component2476109
version: 1
top level: BBa_K1650009
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2476113
display id: component2476113
version: 1
top level: BBa_K1650009
definition: BBa_B0010
access: public

BBa_K1650013
type: Component
persistent identity: component2476123
display id: component2476123
version: 1
top level: BBa_K1650009
definition: CDI
access: public

BBa_K1650034
type: Component
persistent identity: component2476118
display id: component2476118
version: 1
top level: BBa_K1650009
definition: BBa_K1650034
access: public

BBa_B0054
type: Component
persistent identity: component2476128
display id: component2476128
version: 1
top level: BBa_K1650009
definition: BBa_B0054
access: public

BBa_B0053
type: Component
persistent identity: component2476125
display id: component2476125
version: 1
top level: BBa_K1650009
definition: BBa_B0053
access: public

BBa_K823008
type: SequenceAnnotation
persistent identity: annotation2476107
display id: annotation2476107
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476107/range2476107/1
component: BBa_K823008

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476107/range2476107/1
type: Range
persistent identity: range2476107
display id: range2476107
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2476109
display id: annotation2476109
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476109/range2476109/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476109/range2476109/1
type: Range
persistent identity: range2476109
display id: range2476109
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2476112
display id: annotation2476112
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476112/range2476112/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476112/range2476112/1
type: Range
persistent identity: range2476112
display id: range2476112
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 62
end: 767
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2476113
display id: annotation2476113
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476113/range2476113/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476113/range2476113/1
type: Range
persistent identity: range2476113
display id: range2476113
version: 1
top level: BBa_K1650009
direction: direction/forward
start: 776
end: 855
orientation: inline

BBa_K1650033
type: SequenceAnnotation
persistent identity: annotation2476116
display id: annotation2476116
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476116/range2476116/1
component: BBa_K1650033

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476116/range2476116/1
type: Range
persistent identity: range2476116
display id: range2476116
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 864
end: 943
orientation: inline

BBa_K1650034
type: SequenceAnnotation
persistent identity: annotation2476118
display id: annotation2476118
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476118/range2476118/1
component: BBa_K1650034

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476118/range2476118/1
type: Range
persistent identity: range2476118
display id: range2476118
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 952
end: 963
orientation: inline

BBa_K1650013
type: SequenceAnnotation
persistent identity: annotation2476123
display id: annotation2476123
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476123/range2476123/1
component: BBa_K1650013

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476123/range2476123/1
type: Range
persistent identity: range2476123
display id: range2476123
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 970
end: 12387
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2476125
display id: annotation2476125
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476125/range2476125/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476125/range2476125/1
type: Range
persistent identity: range2476125
display id: range2476125
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 12396
end: 12467
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2476128
display id: annotation2476128
version: 1
top level: BBa_K1650009
location: https://synbiohub.org/public/igem/BBa_K1650009/annotation2476128/range2476128/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1650009/annotation2476128/range2476128/1
type: Range
persistent identity: range2476128
display id: range2476128
version: 1
top level: BBa_K1650009
direction: direction/reverse
start: 12476
end: 12544
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_K1650033
type: ComponentDefinition
persistent identity: BBa K1650033
display id: BBa_K1650033
version: 1
was generated by: iGEM to SBOL conversion
description: T5-Promotor
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T10:57:43Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: Promotor
discontinued: false
dominant: false
group u list: _2067_
m user id: 12520
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Alexandra Richter
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_K1650033
sequence: https://synbiohub.org/public/igem/BBa_K1650033_sequence/1

BBa_K1650033
type: SequenceAnnotation
persistent identity: annotation2474906
display id: annotation2474906
version: 1
top level: BBa_K1650033
location: https://synbiohub.org/public/igem/BBa_K1650033/annotation2474906/range2474906/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1650033/annotation2474906/range2474906/1
type: Range
persistent identity: range2474906
display id: range2474906
version: 1
top level: BBa_K1650033
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_K1650034
type: ComponentDefinition
persistent identity: BBa K1650034
display id: BBa_K1650034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T10:59:15Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: (T5)
discontinued: false
dominant: false
group u list: _2067_
m user id: 12520
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Alexandra Richter
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K1650034
sequence: https://synbiohub.org/public/igem/BBa_K1650034_sequence/1

BBa_K1650034
type: SequenceAnnotation
persistent identity: annotation2474905
display id: annotation2474905
version: 1
top level: BBa_K1650034
location: https://synbiohub.org/public/igem/BBa_K1650034/annotation2474905/range2474905/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1650034/annotation2474905/range2474905/1
type: Range
persistent identity: range2474905
display id: range2474905
version: 1
top level: BBa_K1650034
direction: direction/forward
start: 1
end: 12
orientation: inline

CDI
type: ComponentDefinition
persistent identity: BBa K1650013
display id: BBa_K1650013
version: 1
was generated by: iGEM to SBOL conversion
description: This part contains the CDI operon
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:47:35Z
mutable provenance: asdljasd
owned by: james
owned by: myers
mutable description: asldk
discontinued: false
dominant: false
experience: experience/Works
group u list: _2067_
m user id: 27248
owner id: 27248
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: asdkljal
star: false
creator: Daniel Stukenberg
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CdiB
sequence annotation: intergenic region
sequence annotation: CdiA
sequence annotation: CdiI
sequence: https://synbiohub.org/public/igem/BBa_K1650013_sequence/1

CdiB
type: SequenceAnnotation
persistent identity: annotation2473449
display id: annotation2473449
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473449/range2473449/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473449/range2473449/1
type: Range
persistent identity: range2473449
display id: range2473449
version: 1
top level: CDI
direction: direction/forward
start: 1
end: 1767
orientation: inline

intergenic region
type: SequenceAnnotation
persistent identity: annotation2473450
display id: annotation2473450
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473450/range2473450/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473450/range2473450/1
type: Range
persistent identity: range2473450
display id: range2473450
version: 1
top level: CDI
direction: direction/forward
start: 1768
end: 1779
orientation: inline

CdiA
type: SequenceAnnotation
persistent identity: annotation2473452
display id: annotation2473452
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473452/range2473452/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473452/range2473452/1
type: Range
persistent identity: range2473452
display id: range2473452
version: 1
top level: CDI
direction: direction/forward
start: 1780
end: 11178
orientation: inline

CdiI
type: SequenceAnnotation
persistent identity: annotation2473451
display id: annotation2473451
version: 1
top level: CDI
location: https://synbiohub.org/public/igem/BBa_K1650013/annotation2473451/range2473451/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1650013/annotation2473451/range2473451/1
type: Range
persistent identity: range2473451
display id: range2473451
version: 1
top level: CDI
direction: direction/forward
start: 11176
end: 11416
orientation: inline

BBa_K823008
type: ComponentDefinition
persistent identity: BBa K823008
display id: BBa_K823008
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson promoter J23106
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:05Z
mutable provenance: Partsregistry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -35 box
sequence annotation: J23106
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823008_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182528
display id: annotation2182528
version: 1
top level: BBa_K823008
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
type: Range
persistent identity: range2182528
display id: range2182528
version: 1
top level: BBa_K823008
direction: direction/forward
start: 1
end: 6
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2182530
display id: annotation2182530
version: 1
top level: BBa_K823008
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
type: Range
persistent identity: range2182530
display id: range2182530
version: 1
top level: BBa_K823008
direction: direction/forward
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182529
display id: annotation2182529
version: 1
top level: BBa_K823008
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
type: Range
persistent identity: range2182529
display id: range2182529
version: 1
top level: BBa_K823008
direction: direction/forward
start: 24
end: 29
orientation: inline

https://synbiohub.org/public/igem/BBa_K1650009_sequence/1
type: Sequence
persistent identity: BBa K1650009 sequence
display id: BBa_K1650009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaatcataaaaaatttatctgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacactactagagaaggaggaattatactagatgcggcaccggcaggataacttactggcaaacagaacattgttgcccggtatggcttccggtcagtacgtattcaggctctgcactttctctccggtggtacgctatttttccctcctcccctgcctttgtattctttcgttttcgtctccggcagccatgctgtctccgggtgaccgcagtgcaattcagcagcaacagcagcagttgctggatgaaaaccagcgtcagcgtgatgcgctggagcgcagtgcgccgctgaccatcacgccgtctccggaaacgtctgccggtactgaaggtccctgctttacggtgtcaagcattgttgtcagtggggccacccgactgacgtctgcagaaaccgacagactggtggcaccgtgggtgaatcagtgtctgaatatcacggggctgaccgcggtcacggatgccatgacggacagctatatacgccggggatatatcaccagccgggcctttctgacagagcaggacctttcagggggcgtactgcacataacggtcatggaaggcaggctgcagcaaatccgggcggaaggcgctgaccttcctgcccgcaccctgaagatggttttcccgggaatggaggggaaggttctgaacctgcgggatattgagcaggggatggagcagattaatcgtctgcgtacggagccggtacagattgaaatatcgcccggtgaccgtgagggatggtcggtggtgacactgacggcattgccggaatggcctgtcacagggagcgtgggcatcgacaacagcgggcagaagagtaccggtacggggcagttaaatggtgtcctttcctttaataatcctctggggctggctgacaactggtttgtcagcgggggacggagcagtgacttttcggtgtcacatgatgcgaggaattttgccgccggtgtcagtctgccgtatggctataccctggtggattacacgtattcatggagtgactacctcagcaccattgataaccggggctggcggtggcgttccacggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1650033_sequence/1
type: Sequence
persistent identity: BBa K1650033 sequence
display id: BBa_K1650033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatcataaaaaatttatctgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1650034_sequence/1
type: Sequence
persistent identity: BBa K1650034 sequence
display id: BBa_K1650034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaggaatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1650013_sequence/1
type: Sequence
persistent identity: BBa K1650013 sequence
display id: BBa_K1650013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcggcaccggcaggataacttactggcaaacagaacattgttgcccggtatggcttccggtcagtacgtattcaggctctgcactttctctccggtggtacgctatttttccctcctcccctgcctttgtattctttcgttttcgtctccggcagccatgctgtctccgggtgaccgcagtgcaattcagcagcaacagcagcagttgctggatgaaaaccagcgtcagcgtgatgcgctggagcgcagtgcgccgctgaccatcacgccgtctccggaaacgtctgccggtactgaaggtccctgctttacggtgtcaagcattgttgtcagtggggccacccgactgacgtctgcagaaaccgacagactggtggcaccgtgggtgaatcagtgtctgaatatcacggggctgaccgcggtcacggatgccatgacggacagctatatacgccggggatatatcaccagccgggcctttctgacagagcaggacctttcagggggcgtactgcacataacggtcatggaaggcaggctgcagcaaatccgggcggaaggcgctgaccttcctgcccgcaccctgaagatggttttcccgggaatggaggggaaggttctgaacctgcgggatattgagcaggggatggagcagattaatcgtctgcgtacggagccggtacagattgaaatatcgcccggtgaccgtgagggatggtcggtggtgacactgacggcattgccggaatggcctgtcacagggagcgtgggcatcgacaacagcgggcagaagagtaccggtacggggcagttaaatggtgtcctttcctttaataatcctctggggctggctgacaactggtttgtcagcgggggacggagcagtgacttttcggtgtcacatgatgcgaggaattttgccgccggtgtcagtctgccgtatggctataccctggtggattacacgtattcatggagtgactacctcagcaccattgataaccggggctggcggtggcgttccacgggagacctgcagactcaccggctgggactgtcgcatgtcctgttccgtaacggggacatgaagacagcactgaccggaggtctgcagcaccgcattattcacaattatctggatgatgttctgcttcagggcagcagccgtaaactcacttcattttctgtcgggctgaatcacacccacaagtttctggggggggtcggaacactgaatccggtattcacacgggggatgccctggttcggcgcagaaagcgaccacgggaaaaggggagacctgcccgtaaatcagttccggaaatggtcggtgagtgccagttttcagcgccccgtcacggacagggtgtggtggctgaccagcgcttatgcccagtggtcaccggaccgtcttcatggtgtggaacaactgagcctcgggggtgagagttcagtgcgtggctttaaggatcagtatatctccggtaataacggcggttatctgcggaatgagctgtcctggtctctgttctccctgccatatgtgggaactgtccgtgcagtggctgcactggacggcggctggctgcactctgacagcgatgacccgtactcgtccggcacgctgtggggtgctgctgccgggctcagcaccaccagtggccatgtttccggttcgttcactgccggactgcctctggtttacccggactggcttgcccctgaccatctcacggtttactggcgcgttgccgtcgcgttttaagggattattaccatgcatcagcctcccgttcgcttcacttaccgcctgctgagttacctggtcagtgcgattatcgccgggcagccgttgttaccggctgtgggggccgtcatcaccccacaaaacggggccggaatggataaagcggcaaatggtgtgccggtcgtgaacattgccacgccgaacggggccgggatttcgcataaccggtttacggattacaacgtcgggaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823008_sequence/1
type: Sequence
persistent identity: BBa K823008 sequence
display id: BBa_K823008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z