Composite carrying the Lac-inducible CDI-operon,constitutively expressed RFP
The CDI-operon was PCR amplified from genomic DNA of E.coli strain EC93. Other parts were taken from the registry.
Note: This part is similar to BBa_K1650007 except that it does not contain lacI and RFP expression is driven by a constitutive promoter instead of a lac-inducible promoter. The composite part contains a lac-inducible CDI operon which gives your strain the capability to inhibit growth of bacterial cells that express BamA in their outer membrane (most gram negative bacteria). Additionally the part contains mRFP1 (BBa_E1010) driven by constitutive promoter. IPTG-induced cells harboring BBa_K1650009 express CdiB which forms a beta-barrel structure. The CdiA protein is transported through CdiB and is displayed on the cell surface. When getting in contact with BamA of a target cell, the C-terminal domain is delivered into the target cell acting as pore forming protein thus destroying the proton motoric force of the target cell. BBa_K1650009 could be used to improve transformation efficiency. Induced CDI+ cells would kill false positive cells
BBa_K1650009 was toxic for cells in pSB1C3 backbone, presumably because of the high metabolic burden. In accordance with iGEM Headquarter, we submitted BBa_K1650009 in the low copy pSB4C5 backbone
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Transcriptional terminator consisting of a 64 bp stem-loop.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
Conversion of the iGEM parts registry to SBOL2.1