LOCUS       BBa_K1650046            1369 bp    DNA     linear       31-Dec-2025
DEFINITION  SpyCatcher_GFP (+RBS)
ACCESSION   BBa_K1650046
VERSION     BBa_K1650046.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..643
                     /label=BBa_K1650037
     CDS             227..643
                     /label=BBa_K1650037
     CDS             652..1369
                     /label=GFP fusion brick
     misc_feature    652..652
                     /label=deletion of TG
     CDS             652..1369
                     /label=BBa_K125500
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt cttattatca
      241 tcatcaccat caccacgatt atgacattcc taccaccgag aacctctact tccaaggcgc
      301 catggtcgat accctttccg gactgtccag cgaacagggc caatctggcg atatgacgat
      361 cgaagaggac tctgcaacgc acattaaatt tagcaagcgg gatgaggatg gtaaggaact
      421 ggcgggtgcg acgatggaac tgcgggactc gagtggtaag acaatttcga cttggatttc
      481 tgatggtcaa gtcaaagatt tctacttata cccgggtaaa tacacttttg ttgaaaccgc
      541 agcgccggat ggctatgaag tggcaacggc aattaccttc accgttaacg agcagggtca
      601 ggttaccgtg aatggaaagg caaccaaggg cgatgctcat atttactaga gcgtaaagga
      661 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      721 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      781 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      841 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      901 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      961 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1021 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1081 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     1141 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     1201 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     1261 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     1321 acagctgctg ggattacaca tggcatggat gaactataca aataataat
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