LOCUS       BBa_K1650048            1808 bp    DNA     linear       27-Dec-2024
DEFINITION  Plac_RBS_spycatcher-GFP-His
ACCESSION   BBa_K1650048
VERSION     BBa_K1650048.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..643
                     /label=BBa_K1650037
     CDS             227..643
                     /label=BBa_K1650037
     CDS             650..1808
                     /label=BBa_K1650038
     CDS             650..1808
                     /label=BBa_K1650038
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt cttattatca
      241 tcatcaccat caccacgatt atgacattcc taccaccgag aacctctact tccaaggcgc
      301 catggtcgat accctttccg gactgtccag cgaacagggc caatctggcg atatgacgat
      361 cgaagaggac tctgcaacgc acattaaatt tagcaagcgg gatgaggatg gtaaggaact
      421 ggcgggtgcg acgatggaac tgcgggactc gagtggtaag acaatttcga cttggatttc
      481 tgatggtcaa gtcaaagatt tctacttata cccgggtaaa tacacttttg ttgaaaccgc
      541 agcgccggat ggctatgaag tggcaacggc aattaccttc accgttaacg agcagggtca
      601 ggttaccgtg aatggaaagg caaccaaggg cgatgctcat atttactaga tgtcttatta
      661 tcatcatcac catcaccacg attatgacat tcctaccacc gagaacctct acttccaagg
      721 cgccatggtc gatacccttt ccggactgtc cagcgaacag ggccaatctg gcgatatgac
      781 gatcgaagag gactctgcaa cgcacattaa atttagcaag cgggatgagg atggtaagga
      841 actggcgggt gcgacgatgg aactgcggga ctcgagtggt aagacaattt cgacttggat
      901 ttctgatggt caagtcaaag atttctactt atacccgggt aaatacactt ttgttgaaac
      961 cgcagcgccg gatggctatg aagtggcaac ggcaattacc ttcaccgtta acgagcaggg
     1021 tcaggttacc gtgaatggaa aggcaaccaa gggcgatgct catattacta gacgtaaagg
     1081 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     1141 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     1201 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     1261 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
     1321 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1381 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     1441 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     1501 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     1561 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     1621 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     1681 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     1741 aacagctgct gggattacac atggcatgga tgaactatac aaacaccacc accaccacca
     1801 ctaataat
//