LOCUS       BBa_K1650049            1696 bp    DNA     linear       24-Nov-2024
DEFINITION  Plac_RBS_spycatcher-ADHII
ACCESSION   BBa_K1650049
VERSION     BBa_K1650049.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..643
                     /label=BBa_K1650037
     CDS             227..643
                     /label=BBa_K1650037
     CDS             650..1696
                     /label=BBa_K1650043
     CDS             650..1696
                     /label=BBa_K1650043
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt cttattatca
      241 tcatcaccat caccacgatt atgacattcc taccaccgag aacctctact tccaaggcgc
      301 catggtcgat accctttccg gactgtccag cgaacagggc caatctggcg atatgacgat
      361 cgaagaggac tctgcaacgc acattaaatt tagcaagcgg gatgaggatg gtaaggaact
      421 ggcgggtgcg acgatggaac tgcgggactc gagtggtaag acaatttcga cttggatttc
      481 tgatggtcaa gtcaaagatt tctacttata cccgggtaaa tacacttttg ttgaaaccgc
      541 agcgccggat ggctatgaag tggcaacggc aattaccttc accgttaacg agcagggtca
      601 ggttaccgtg aatggaaagg caaccaaggg cgatgctcat atttactaga tgtctattcc
      661 agaaactcaa aaagccatta tcttctacga atccaacggc aagttggagc ataaggatat
      721 cccagttcca aagccaaagc ccaacgaatt gttaatcaac gtcaagtact ctggtgtctg
      781 ccacaccgat ttgcacgctt ggcatggtga ctggccattg ccaactaagt taccattagt
      841 tggtggtcac gaaggtgccg gtgtcgttgt cggcatgggt gaaaacgtta agggctggaa
      901 gatcggtgac tacgccggta tcaaatggtt gaacggttct tgtatggcct gtgaatactg
      961 tgaattgggt aacgaatcca actgtcctca cgctgacttg tctggttaca cccacgacgg
     1021 ttctttccaa gaatacgcta ccgctgacgc tgttcaagcc gctcacattc ctcaaggtac
     1081 tgacttggct gaagtcgcgc caatcttgtg tgctggtatc accgtataca aggctttgaa
     1141 gtctgccaac ttgagagcag gccactgggc ggccatttct ggtgctgctg gtggtctagg
     1201 ttctttggct gttcaatatg ctaaggcgat gggttacaga gtcttaggta ttgatggtgg
     1261 tccaggaaag gaagaattgt ttacctcgct cggtggtgaa gtattcatcg acttcaccaa
     1321 agagaaggac attgttagcg cagtcgttaa ggctaccaac ggcggtgccc acggtatcat
     1381 caatgtttcc gtttccgaag ccgctatcga agcttctacc agatactgta gggcgaacgg
     1441 tactgttgtc ttggttggtt tgccagccgg tgcaaagtgc tcctctgatg tcttcaacca
     1501 cgttgtcaag tctatctcca ttgtcggctc ttacgtgggg aacagagctg ataccagaga
     1561 agccttagat ttctttgcca gaggtctagt caagtctcca ataaaggtag ttggcttatc
     1621 cagtttacca gaaatttacg aaaagatgga gaagggccaa attgctggta gatacgttgt
     1681 tgacacttct aaataa
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