LOCUS       BBa_K1650053            1647 bp    DNA     linear       28-Dec-2024
DEFINITION  PlacI_RBS_SpyCatcher-GGBP
ACCESSION   BBa_K1650053
VERSION     BBa_K1650053.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..643
                     /label=BBa_K1650037
     CDS             227..643
                     /label=BBa_K1650037
     CDS             652..1647
                     /label=BBa_K1650042
     CDS             652..1647
                     /label=BBa_K1650042
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt cttattatca
      241 tcatcaccat caccacgatt atgacattcc taccaccgag aacctctact tccaaggcgc
      301 catggtcgat accctttccg gactgtccag cgaacagggc caatctggcg atatgacgat
      361 cgaagaggac tctgcaacgc acattaaatt tagcaagcgg gatgaggatg gtaaggaact
      421 ggcgggtgcg acgatggaac tgcgggactc gagtggtaag acaatttcga cttggatttc
      481 tgatggtcaa gtcaaagatt tctacttata cccgggtaaa tacacttttg ttgaaaccgc
      541 agcgccggat ggctatgaag tggcaacggc aattaccttc accgttaacg agcagggtca
      601 ggttaccgtg aatggaaagg caaccaaggg cgatgctcat atttactaga gaataagaag
      661 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
      721 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
      781 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
      841 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
      901 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
      961 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
     1021 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcaaggcgat
     1081 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
     1141 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
     1201 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
     1261 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
     1321 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggttgaagcg
     1381 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
     1441 ctggcgctgg tgaaatccgg tgcactggcg ggcaccgtac tgaacgatgc taacaaccag
     1501 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gtaaaggtgc ggctgatggc
     1561 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
     1621 aacctggctg agttcagcaa gaaataa
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