BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K165023 1 BBa_K165023 Kozak + Zif268-HIV synthetic activator (untagged) 2008-10-27T12:00:00Z 2015-05-08T01:10:55Z - This is the kozak ribosome binding sequence ligated onto the Zif268-HIV synthetic transcriptional activator (BBa_K165022). false false _267_ 0 2512 58 It's complicated false - false John Szymanski component1992131 1 BBa_K165022 component1992130 1 BBa_K165002 annotation1992131 1 BBa_K165022 range1992131 1 27 692 annotation1992130 1 BBa_K165002 range1992130 1 1 18 BBa_K165022 1 BBa_K165022 Zif268-HIV synthetic activator (untagged) 2008-10-27T12:00:00Z 2015-05-08T01:10:55Z From David Drubin in the Silver Lab at Harvard. This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the Zif268-HIV DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Zif268-HIV binding sites, activating transcription downstream. false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K165002_sequence 1 cccgccgccaccatggag BBa_K165023_sequence 1 cccgccgccaccatggagtactagagcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaaactagagacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgactagacccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K165022_sequence 1 cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaaactagagacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgactagacccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z