BBa_K165030
1
BBa_K165030
mCYC promoter plus Zif268-HIV binding sites
2008-10-28T12:00:00Z
2015-05-08T01:10:56Z
Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.
false
false
_267_
0
2510
58
It's complicated
false
It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
false
Aaron Glieberman
annotation1994037
1
Zif268-HIV binding sites (3)
range1994037
1
1
46
BBa_K165030_sequence
1
ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgtagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z