BBa_K165034
1
BBa_K165034
Zif268-HIV bs + LexA bs + mCYC promoter
2008-10-29T12:00:00Z
2015-05-08T01:10:56Z
Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
false
false
_267_
0
2512
58
It's complicated
false
-
true
John Szymanski
component1995464
1
BBa_K165011
component1995465
1
BBa_K165031
annotation1995465
1
BBa_K165031
range1995465
1
55
457
annotation1995464
1
BBa_K165011
range1995464
1
1
46
BBa_K165011
1
BBa_K165011
Zif268-HIV binding sites (3)
2008-10-25T11:00:00Z
2015-05-08T01:10:55Z
-
-
false
false
_267_
0
2512
58
It's complicated
false
-
false
John Szymanski
BBa_K165031
1
BBa_K165031
mCYC promoter plus LexA binding sites
2008-10-28T12:00:00Z
2015-05-08T01:10:56Z
Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain.
false
false
_267_
0
2510
58
It's complicated
false
It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
false
Aaron Glieberman
BBa_K165031_sequence
1
ctgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
BBa_K165034_sequence
1
ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagtactagagctgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
BBa_K165011_sequence
1
ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z