BBa_K165030 1 BBa_K165030 mCYC promoter plus Zif268-HIV binding sites 2008-10-28T12:00:00Z 2015-05-08T01:10:56Z Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory. This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA. false false _267_ 0 2510 58 It's complicated false It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector. false Aaron Glieberman annotation1994037 1 Zif268-HIV binding sites (3) range1994037 1 1 46 BBa_K165012 1 BBa_K165012 Gli1 binding sites 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - - false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K165035 1 BBa_K165035 Gli1 bs + Zif268-HIV bs + mCYC promoter 2008-10-29T12:00:00Z 2015-05-08T01:10:56Z Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin. The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors. false false _267_ 0 2512 58 It's complicated false - false John Szymanski component1995495 1 BBa_K165012 component1995497 1 BBa_K165030 annotation1995497 1 BBa_K165030 range1995497 1 136 442 annotation1995495 1 BBa_K165012 range1995495 1 1 127 BBa_K165035_sequence 1 ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagtactagagctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgtagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag BBa_K165030_sequence 1 ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgtagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag BBa_K165012_sequence 1 ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z