LOCUS BBa_K165050 5117 bp DNA linear 22-Nov-2024 DEFINITION Full Gli1 Repressor (tagged with mCherryx2) ACCESSION BBa_K165050 VERSION BBa_K165050.1 FEATURES Location/Qualifiers RBS 1..18 /label=BBa_K165002 misc_feature 27..2837 /label=BBa_K165003 misc_feature 2846..3370 /label=BBa_K165007 misc_feature 3379..4113 /label=BBa_K165004 misc_feature 4122..4856 /label=BBa_K165004 terminator 4865..5117 /label=BBa_K165018 ORIGIN 1 cccgccgcca ccatggagta ctagagagaa cgtctctcac tgaacagtac gctccttcta 61 gtatacaaca tcagcagcag cacccgcaaa gtattagtcc tatagcaaat acgcagtatg 121 gtgatatccc tgttagaccg gaaattgatt tagatcctag cattgtgcct gtggtccctg 181 aacccactga gcccatcgaa aacaatatat cgcttaatga ggaagtcact ttcttcgaaa 241 aggccaagag gtatatcggc aataaacatt tatacactga gtttttgaaa attttaaatt 301 tgtactctca agatatactt gatcttgacg atttagtgga aaaggtagat ttctacttgg 361 gttccaataa agaactattt acgtggttca aaaactttgt tggctaccaa gaaaaaacca 421 aatgtatcga gaatattgtt catgaaaaac atagattgga tttagattta tgtgaggcat 481 ttggcccaag ttacaagagg ctaccaaaaa gtgacacttt catgccatgc tcaggtaggg 541 atgatatgtg ttgggaagtc ttgaacgatg aatgggttgg acatcctgta tgggcttccg 601 aagattcggg atttattgct catcgtaaaa accagtatga ggaaacacta ttcaagatcg 661 aagaggaaag acatgagtat gatttttaca ttgaatcaaa tttaagaact attcaatgtt 721 tggaaacaat tgtaaataag atcgagaaca tgactgagaa tgaaaaagcc aattttaaac 781 tgcctccagg tcttggccat acttcaatga ctatttataa aaaagtgata aggaaagttt 841 atgataagga aagggggttc gagattattg atgctttgca tgagcaccct gcggtgacag 901 ccccagtcgt tctgaaaagg ttaaagcaaa aagacgaaga atggagaaga gctcaacgtg 961 aatggaataa agtttggagg gagttagaac agaaggtttt tttcaagtca ttagatcatt 1021 taggcttaac atttaaacag gctgacaaga aattattaac tacaaagcag ttgatatcag 1081 agattagcag catcaaagtt gatcaaacaa acaaaaaaat tcactggtta actcctaaac 1141 caaagagcca gttagatttt gatttccctg ataaaaacat tttctatgat atcttgtgtt 1201 tggctgacac ttttataacc cataccacag cctattctaa tcccgataaa gaaagattga 1261 aagatttact aaaatacttc atctctttgt ttttttctat ttctttcgaa aaaatcgaag 1321 aatcgttgta ctcccataag caaaacgtgt cagaatctag cggatctgac gatggcagtt 1381 ctattgcatc aaggaagagg ccctatcaac aagaaatgag tttacttgat attttacaca 1441 ggagcagata tcaaaagcta aagcgttcta atgatgaaga tggcaaagtt ccccagctct 1501 ctgaaccacc cgaagaagaa cctaatacca ttgaggagga agagctcatc gatgaagaag 1561 ctaaaaatcc gtggctaact gggaatttag tggaggaagc aaactctcag ggtattatcc 1621 aaaatcgcag tatttttaat ctattcgcta atacgaatat ttacatattt ttccgtcatt 1681 ggacaacgat ttatgagcgg cttttggaaa ttaagcaaat gaatgaaagg gtcacaaagg 1741 aaatcaacac aaggtcgaca gttacttttg ccaaagatct ggatttatta tcgagtcaac 1801 tttccgaaat gggactagat tttgttggtg aagacgcgta caaacaggtt ttaagactga 1861 gtagaaggtt gattaatggg gatcttgaac atcagtggtt tgaagagagt ttgcggcaag 1921 cttacaataa caaagcgttt aaactctata caattgataa agtcacccaa tcgttggtaa 1981 agcatgctca taccttgatg actgacgcta aaactgcgga aataatggct ttgttcgtta 2041 aagatagaaa tgcctccacc acgagtgcga aggaccaaat tatctatcgc ttgcaggtgc 2101 gctcacatat gtccaacaca gaaaatatgt ttagaataga gtttgataaa agaactctgc 2161 atgtttccat tcaatatatt gcacttgatg atttgacact aaaggaacca aaggcagacg 2221 aagataaatg gaaatattat gtaacatcgt atgctctgcc acatccaaca gaaggcattc 2281 cgcacgagaa actgaagata ccatttttgg aaaggctcat cgaatttgga caagatattg 2341 atggaacaga ggtagatgag gagttttccc ccgaaggcat ctctgtatcg acattgaaaa 2401 ttaagatcca gcctataacc taccaactgc atattgaaaa cgggtcttac gatgttttta 2461 cccgtaaggc tactaacaaa tatcctacta ttgctaatga taatactcaa aaaggaatgg 2521 tttctcagaa gaaggagttg atatcaaaat ttctggactg tgcagttggt ttgagaaata 2581 atttagatga ggctcaaaaa ttaagtatgc aaaaaaaatg ggagaaccta aaagacagca 2641 tagcaaagac gagtgctgga aaccagggaa tagagagtga aaccgaaaaa ggtaaaatta 2701 cgaaacagga gcagtcggat aatttggaca gctctactgc aagtgtactg cctgcatcca 2761 ttactactgt accacaagat gataatatag aaacgactgg gaatactgaa tcttcagaca 2821 agggggctaa gattcaatac tagagaagcg tgagcctgaa tctgtgtatg aaactgactg 2881 ccgttgggat ggctgtagcc aggaatttga ctcccaagag cagctggtgc accacatcaa 2941 cagcgagcac atccacgggg agcggaagga gttcgtgtgc cactgggggg gctgctccag 3001 ggagctgagg cccttcaaag cccagtacat gctggtggtt cacatgcgca gacacactgg 3061 cgagaagcca cacaagtgca cgtttgaagg gtgccggaag tcatactcac gcctcgaaaa 3121 cctgaagacg cacctgcggt cacacacggg tgagaagcca tacatgtgtg agcacgaggg 3181 ctgtagtaaa gccttcagca atgccagtga ccgagccaag caccagaatc ggacccattc 3241 caatgagaag ccgtatgtat gtaagctccc tggctgcacc aaacgctata cagatcctag 3301 ctcgctgcga aaacatgtca agacagtgca tggtcctgac gcccatgtga ccaaacggca 3361 ccgtggggat tactagaggc aactagcggc atggttagta aaggagaaga aaataacatg 3421 gcaatcatta aggagttcat gagattcaaa gttcacatgg aaggttctgt aaatggacat 3481 gaatttgaaa tagaaggtga aggagaagga aggccttatg aaggaaccca aaccgcgaag 3541 ctaaaagtta ctaagggtgg cccattacca tttgcatggg atatccttag ccctcaattc 3601 atgtatgggt caaaggctta tgtcaagcac cccgccgaca ttccagacta tctaaagtta 3661 tcttttcccg aagggtttaa gtgggagcgt gtgatgaact tcgaagacgg tggcgtggta 3721 acagtgactc aggattcgtc cctgcaagat ggtgaattta tctacaaagt caaattaaga 3781 ggaactaact ttccatctga cggcccggtt atgcaaaaaa agacaatggg ctgggaggcc 3841 tcctcagaac gaatgtaccc tgaagatggt gccttgaagg gtgagattaa acaaagattg 3901 aaattgaaag atggtggaca ttatgacgct gaggttaaaa cgacatacaa agctaagaaa 3961 cctgtccagc tcccaggtgc ttacaatgta aatataaaac ttgatattac atcacataat 4021 gaagattata cgatagttga acaatacgaa agggctgagg ggagacatag tactggtggc 4081 atggatgaac tatacaaagg ttctggtacc gcatactaga ggcaactagc ggcatggtta 4141 gtaaaggaga agaaaataac atggcaatca ttaaggagtt catgagattc aaagttcaca 4201 tggaaggttc tgtaaatgga catgaatttg aaatagaagg tgaaggagaa ggaaggcctt 4261 atgaaggaac ccaaaccgcg aagctaaaag ttactaaggg tggcccatta ccatttgcat 4321 gggatatcct tagccctcaa ttcatgtatg ggtcaaaggc ttatgtcaag caccccgccg 4381 acattccaga ctatctaaag ttatcttttc ccgaagggtt taagtgggag cgtgtgatga 4441 acttcgaaga cggtggcgtg gtaacagtga ctcaggattc gtccctgcaa gatggtgaat 4501 ttatctacaa agtcaaatta agaggaacta actttccatc tgacggcccg gttatgcaaa 4561 aaaagacaat gggctgggag gcctcctcag aacgaatgta ccctgaagat ggtgccttga 4621 agggtgagat taaacaaaga ttgaaattga aagatggtgg acattatgac gctgaggtta 4681 aaacgacata caaagctaag aaacctgtcc agctcccagg tgcttacaat gtaaatataa 4741 aacttgatat tacatcacat aatgaagatt atacgatagt tgaacaatac gaaagggctg 4801 aggggagaca tagtactggt ggcatggatg aactatacaa aggttctggt accgcatact 4861 agagcccaag aaaaagcgca aggtaactag ataataagcg aatttcttat gatttatgat 4921 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta 4981 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc 5041 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg 5101 cctgcaaatc gctccct //