LOCUS BBa_K165054 5133 bp DNA linear 14-Nov-2024 DEFINITION Full Gli1 Repressor (tagged with CFPx2) ACCESSION BBa_K165054 VERSION BBa_K165054.1 FEATURES Location/Qualifiers RBS 1..18 /label=BBa_K165002 misc_feature 27..2837 /label=BBa_K165003 misc_feature 2846..3370 /label=BBa_K165007 misc_feature 3379..4872 /label=BBa_K165014 terminator 4881..5133 /label=BBa_K165018 ORIGIN 1 cccgccgcca ccatggagta ctagagagaa cgtctctcac tgaacagtac gctccttcta 61 gtatacaaca tcagcagcag cacccgcaaa gtattagtcc tatagcaaat acgcagtatg 121 gtgatatccc tgttagaccg gaaattgatt tagatcctag cattgtgcct gtggtccctg 181 aacccactga gcccatcgaa aacaatatat cgcttaatga ggaagtcact ttcttcgaaa 241 aggccaagag gtatatcggc aataaacatt tatacactga gtttttgaaa attttaaatt 301 tgtactctca agatatactt gatcttgacg atttagtgga aaaggtagat ttctacttgg 361 gttccaataa agaactattt acgtggttca aaaactttgt tggctaccaa gaaaaaacca 421 aatgtatcga gaatattgtt catgaaaaac atagattgga tttagattta tgtgaggcat 481 ttggcccaag ttacaagagg ctaccaaaaa gtgacacttt catgccatgc tcaggtaggg 541 atgatatgtg ttgggaagtc ttgaacgatg aatgggttgg acatcctgta tgggcttccg 601 aagattcggg atttattgct catcgtaaaa accagtatga ggaaacacta ttcaagatcg 661 aagaggaaag acatgagtat gatttttaca ttgaatcaaa tttaagaact attcaatgtt 721 tggaaacaat tgtaaataag atcgagaaca tgactgagaa tgaaaaagcc aattttaaac 781 tgcctccagg tcttggccat acttcaatga ctatttataa aaaagtgata aggaaagttt 841 atgataagga aagggggttc gagattattg atgctttgca tgagcaccct gcggtgacag 901 ccccagtcgt tctgaaaagg ttaaagcaaa aagacgaaga atggagaaga gctcaacgtg 961 aatggaataa agtttggagg gagttagaac agaaggtttt tttcaagtca ttagatcatt 1021 taggcttaac atttaaacag gctgacaaga aattattaac tacaaagcag ttgatatcag 1081 agattagcag catcaaagtt gatcaaacaa acaaaaaaat tcactggtta actcctaaac 1141 caaagagcca gttagatttt gatttccctg ataaaaacat tttctatgat atcttgtgtt 1201 tggctgacac ttttataacc cataccacag cctattctaa tcccgataaa gaaagattga 1261 aagatttact aaaatacttc atctctttgt ttttttctat ttctttcgaa aaaatcgaag 1321 aatcgttgta ctcccataag caaaacgtgt cagaatctag cggatctgac gatggcagtt 1381 ctattgcatc aaggaagagg ccctatcaac aagaaatgag tttacttgat attttacaca 1441 ggagcagata tcaaaagcta aagcgttcta atgatgaaga tggcaaagtt ccccagctct 1501 ctgaaccacc cgaagaagaa cctaatacca ttgaggagga agagctcatc gatgaagaag 1561 ctaaaaatcc gtggctaact gggaatttag tggaggaagc aaactctcag ggtattatcc 1621 aaaatcgcag tatttttaat ctattcgcta atacgaatat ttacatattt ttccgtcatt 1681 ggacaacgat ttatgagcgg cttttggaaa ttaagcaaat gaatgaaagg gtcacaaagg 1741 aaatcaacac aaggtcgaca gttacttttg ccaaagatct ggatttatta tcgagtcaac 1801 tttccgaaat gggactagat tttgttggtg aagacgcgta caaacaggtt ttaagactga 1861 gtagaaggtt gattaatggg gatcttgaac atcagtggtt tgaagagagt ttgcggcaag 1921 cttacaataa caaagcgttt aaactctata caattgataa agtcacccaa tcgttggtaa 1981 agcatgctca taccttgatg actgacgcta aaactgcgga aataatggct ttgttcgtta 2041 aagatagaaa tgcctccacc acgagtgcga aggaccaaat tatctatcgc ttgcaggtgc 2101 gctcacatat gtccaacaca gaaaatatgt ttagaataga gtttgataaa agaactctgc 2161 atgtttccat tcaatatatt gcacttgatg atttgacact aaaggaacca aaggcagacg 2221 aagataaatg gaaatattat gtaacatcgt atgctctgcc acatccaaca gaaggcattc 2281 cgcacgagaa actgaagata ccatttttgg aaaggctcat cgaatttgga caagatattg 2341 atggaacaga ggtagatgag gagttttccc ccgaaggcat ctctgtatcg acattgaaaa 2401 ttaagatcca gcctataacc taccaactgc atattgaaaa cgggtcttac gatgttttta 2461 cccgtaaggc tactaacaaa tatcctacta ttgctaatga taatactcaa aaaggaatgg 2521 tttctcagaa gaaggagttg atatcaaaat ttctggactg tgcagttggt ttgagaaata 2581 atttagatga ggctcaaaaa ttaagtatgc aaaaaaaatg ggagaaccta aaagacagca 2641 tagcaaagac gagtgctgga aaccagggaa tagagagtga aaccgaaaaa ggtaaaatta 2701 cgaaacagga gcagtcggat aatttggaca gctctactgc aagtgtactg cctgcatcca 2761 ttactactgt accacaagat gataatatag aaacgactgg gaatactgaa tcttcagaca 2821 agggggctaa gattcaatac tagagaagcg tgagcctgaa tctgtgtatg aaactgactg 2881 ccgttgggat ggctgtagcc aggaatttga ctcccaagag cagctggtgc accacatcaa 2941 cagcgagcac atccacgggg agcggaagga gttcgtgtgc cactgggggg gctgctccag 3001 ggagctgagg cccttcaaag cccagtacat gctggtggtt cacatgcgca gacacactgg 3061 cgagaagcca cacaagtgca cgtttgaagg gtgccggaag tcatactcac gcctcgaaaa 3121 cctgaagacg cacctgcggt cacacacggg tgagaagcca tacatgtgtg agcacgaggg 3181 ctgtagtaaa gccttcagca atgccagtga ccgagccaag caccagaatc ggacccattc 3241 caatgagaag ccgtatgtat gtaagctccc tggctgcacc aaacgctata cagatcctag 3301 ctcgctgcga aaacatgtca agacagtgca tggtcctgac gcccatgtga ccaaacggca 3361 ccgtggggat tactagaggc aactagcggc atggttagta aaggagaaga acttttcact 3421 ggagttgtcc caattctggt tgaattggat ggcgatgtca atggccataa gttttcggtt 3481 agtggtgaag gagagggcga cgctacctat gggaagttaa ctttaaagtt catttgtact 3541 accggtaagt taccagttcc ttggcctact ttggtcacaa cccttacatg gggggtgcag 3601 tgctttgcca gatatccgga tcacatgaaa caacacgatt ttttcaaatc cgctatgcct 3661 gaaggatatg tacaagaaag aaccatattt ttcaaggatg acggcaacta caaaactaga 3721 gccgaagtta aattcgaagg tgacacattg gtaaatcgaa ttgagctcaa aggaatagat 3781 tttaaggaag atggtaacat ccttggtcat aagttagagt ataatgcaat ttctgataac 3841 gtctacataa ctgcggataa acagaaaaat ggtattaaag ccaattttaa aattaggcat 3901 aacatcgaag atgggagtgt tcaacttgca gaccactacc aacaaaatac acccatagga 3961 gacggtcccg tactgttgcc agataaccat tatctgtcta cacaatctaa attaagcaaa 4021 gatccaaatg aaaagcgtga ccacatggtg ttgctagagt ttgtaacagc tgctgggatt 4081 acacatggca tggatgaact atacaaaggt tctggtaccg caactagagc aactagcggc 4141 atggttagta aaggagaaga acttttcact ggagttgtcc caattctggt tgaattggat 4201 ggcgatgtca atggccataa gttttcggtt agtggtgaag gagagggcga cgctacctat 4261 gggaagttaa ctttaaagtt catttgtact accggtaagt taccagttcc ttggcctact 4321 ttggtcacaa cccttacatg gggggtgcag tgctttgcca gatatccgga tcacatgaaa 4381 caacacgatt ttttcaaatc cgctatgcct gaaggatatg tacaagaaag aaccatattt 4441 ttcaaggatg acggcaacta caaaactaga gccgaagtta aattcgaagg tgacacattg 4501 gtaaatcgaa ttgagctcaa aggaatagat tttaaggaag atggtaacat ccttggtcat 4561 aagttagagt ataatgcaat ttctgataac gtctacataa ctgcggataa acagaaaaat 4621 ggtattaaag ccaattttaa aattaggcat aacatcgaag atgggagtgt tcaacttgca 4681 gaccactacc aacaaaatac acccatagga gacggtcccg tactgttgcc agataaccat 4741 tatctgtcta cacaatctaa attaagcaaa gatccaaatg aaaagcgtga ccacatggtg 4801 ttgctagagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaaggt 4861 tctggtaccg catactagag cccaagaaaa agcgcaaggt aactagataa taagcgaatt 4921 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat 4981 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt 5041 aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg 5101 gcatgccgag caaatgcctg caaatcgctc cct //