BBa_K165002
1
BBa_K165002
Kozak sequence (yeast RBS)
2008-10-25T11:00:00Z
2015-05-08T01:10:55Z
-
Released HQ 2013
The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
false
false
_267_
0
2512
58
In stock
false
-
false
John Szymanski
BBa_K165054
1
BBa_K165054
Full Gli1 Repressor (tagged with CFPx2)
2008-10-29T12:00:00Z
2015-05-08T01:10:56Z
-
Kozak+Sin3p RD+Gli1 DBD+CFPx2+SV40NLS+tADH1
false
false
_267_
0
2512
58
It's complicated
true
-
false
John Szymanski
component1996737
1
BBa_K165007
component1996735
1
BBa_K165002
component1996738
1
BBa_K165014
component1996739
1
BBa_K165018
component1996736
1
BBa_K165003
annotation1996737
1
BBa_K165007
range1996737
1
2846
3370
annotation1996735
1
BBa_K165002
range1996735
1
1
18
annotation1996738
1
BBa_K165014
range1996738
1
3379
4872
annotation1996739
1
BBa_K165018
range1996739
1
4881
5133
annotation1996736
1
BBa_K165003
range1996736
1
27
2837
BBa_K165003
1
Sin3
Sin3 Repression Domain
2008-10-25T11:00:00Z
2015-05-08T01:10:55Z
From the yeast genome, via Caroline Ajo-Franklin.
This is a transcriptional repression domain. It can be fused to many DNA-binding domains to create synthetic transcriptional repressors. We have used it upstream of DNA binding domains.
false
false
_267_
0
2512
58
It's complicated
true
-
false
John Szymanski
BBa_K165007
1
BBa_K165007
Gli-1 DNA-binding domain
2008-10-25T11:00:00Z
2015-05-08T01:10:55Z
-
-
false
false
_267_
0
2512
58
It's complicated
true
-
false
John Szymanski
BBa_K165014
1
cfpx2
CFP x2
2008-10-25T11:00:00Z
2015-05-08T01:10:55Z
From David Drubin in the Silver Lab at Harvard.
This is 2 copies of the CFP gene fused to each other. The duplex FP tends to be more stable, and is used in synthetic transcription factors to provide a fluorescent output of concentration as well as stability.
false
false
_267_
0
2512
58
It's complicated
true
-
false
John Szymanski
BBa_K165018
1
NLS+Term
SV40 nuclear localization sequence + ADH1 transcription terminator
2008-10-25T11:00:00Z
2015-05-08T01:10:55Z
-
The SV40 nuclear localization sequence can be added to any coding region to have it expressed in the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences.
false
false
_267_
0
2512
58
It's complicated
true
-
true
John Szymanski
BBa_K165002_sequence
1
cccgccgccaccatggag
BBa_K165003_sequence
1
agaacgtctctcactgaacagtacgctccttctagtatacaacatcagcagcagcacccgcaaagtattagtcctatagcaaatacgcagtatggtgatatccctgttagaccggaaattgatttagatcctagcattgtgcctgtggtccctgaacccactgagcccatcgaaaacaatatatcgcttaatgaggaagtcactttcttcgaaaaggccaagaggtatatcggcaataaacatttatacactgagtttttgaaaattttaaatttgtactctcaagatatacttgatcttgacgatttagtggaaaaggtagatttctacttgggttccaataaagaactatttacgtggttcaaaaactttgttggctaccaagaaaaaaccaaatgtatcgagaatattgttcatgaaaaacatagattggatttagatttatgtgaggcatttggcccaagttacaagaggctaccaaaaagtgacactttcatgccatgctcaggtagggatgatatgtgttgggaagtcttgaacgatgaatgggttggacatcctgtatgggcttccgaagattcgggatttattgctcatcgtaaaaaccagtatgaggaaacactattcaagatcgaagaggaaagacatgagtatgatttttacattgaatcaaatttaagaactattcaatgtttggaaacaattgtaaataagatcgagaacatgactgagaatgaaaaagccaattttaaactgcctccaggtcttggccatacttcaatgactatttataaaaaagtgataaggaaagtttatgataaggaaagggggttcgagattattgatgctttgcatgagcaccctgcggtgacagccccagtcgttctgaaaaggttaaagcaaaaagacgaagaatggagaagagctcaacgtgaatggaataaagtttggagggagttagaacagaaggtttttttcaagtcattagatcatttaggcttaacatttaaacaggctgacaagaaattattaactacaaagcagttgatatcagagattagcagcatcaaagttgatcaaacaaacaaaaaaattcactggttaactcctaaaccaaagagccagttagattttgatttccctgataaaaacattttctatgatatcttgtgtttggctgacacttttataacccataccacagcctattctaatcccgataaagaaagattgaaagatttactaaaatacttcatctctttgtttttttctatttctttcgaaaaaatcgaagaatcgttgtactcccataagcaaaacgtgtcagaatctagcggatctgacgatggcagttctattgcatcaaggaagaggccctatcaacaagaaatgagtttacttgatattttacacaggagcagatatcaaaagctaaagcgttctaatgatgaagatggcaaagttccccagctctctgaaccacccgaagaagaacctaataccattgaggaggaagagctcatcgatgaagaagctaaaaatccgtggctaactgggaatttagtggaggaagcaaactctcagggtattatccaaaatcgcagtatttttaatctattcgctaatacgaatatttacatatttttccgtcattggacaacgatttatgagcggcttttggaaattaagcaaatgaatgaaagggtcacaaaggaaatcaacacaaggtcgacagttacttttgccaaagatctggatttattatcgagtcaactttccgaaatgggactagattttgttggtgaagacgcgtacaaacaggttttaagactgagtagaaggttgattaatggggatcttgaacatcagtggtttgaagagagtttgcggcaagcttacaataacaaagcgtttaaactctatacaattgataaagtcacccaatcgttggtaaagcatgctcataccttgatgactgacgctaaaactgcggaaataatggctttgttcgttaaagatagaaatgcctccaccacgagtgcgaaggaccaaattatctatcgcttgcaggtgcgctcacatatgtccaacacagaaaatatgtttagaatagagtttgataaaagaactctgcatgtttccattcaatatattgcacttgatgatttgacactaaaggaaccaaaggcagacgaagataaatggaaatattatgtaacatcgtatgctctgccacatccaacagaaggcattccgcacgagaaactgaagataccatttttggaaaggctcatcgaatttggacaagatattgatggaacagaggtagatgaggagttttcccccgaaggcatctctgtatcgacattgaaaattaagatccagcctataacctaccaactgcatattgaaaacgggtcttacgatgtttttacccgtaaggctactaacaaatatcctactattgctaatgataatactcaaaaaggaatggtttctcagaagaaggagttgatatcaaaatttctggactgtgcagttggtttgagaaataatttagatgaggctcaaaaattaagtatgcaaaaaaaatgggagaacctaaaagacagcatagcaaagacgagtgctggaaaccagggaatagagagtgaaaccgaaaaaggtaaaattacgaaacaggagcagtcggataatttggacagctctactgcaagtgtactgcctgcatccattactactgtaccacaagatgataatatagaaacgactgggaatactgaatcttcagacaagggggctaagattcaa
BBa_K165014_sequence
1
gcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattctggttgaattggatggcgatgtcaatggccataagttttcggttagtggtgaaggagagggcgacgctacctatgggaagttaactttaaagttcatttgtactaccggtaagttaccagttccttggcctactttggtcacaacccttacatggggggtgcagtgctttgccagatatccggatcacatgaaacaacacgattttttcaaatccgctatgcctgaaggatatgtacaagaaagaaccatatttttcaaggatgacggcaactacaaaactagagccgaagttaaattcgaaggtgacacattggtaaatcgaattgagctcaaaggaatagattttaaggaagatggtaacatccttggtcataagttagagtataatgcaatttctgataacgtctacataactgcggataaacagaaaaatggtattaaagccaattttaaaattaggcataacatcgaagatgggagtgttcaacttgcagaccactaccaacaaaatacacccataggagacggtcccgtactgttgccagataaccattatctgtctacacaatctaaattaagcaaagatccaaatgaaaagcgtgaccacatggtgttgctagagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcaactagagcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattctggttgaattggatggcgatgtcaatggccataagttttcggttagtggtgaaggagagggcgacgctacctatgggaagttaactttaaagttcatttgtactaccggtaagttaccagttccttggcctactttggtcacaacccttacatggggggtgcagtgctttgccagatatccggatcacatgaaacaacacgattttttcaaatccgctatgcctgaaggatatgtacaagaaagaaccatatttttcaaggatgacggcaactacaaaactagagccgaagttaaattcgaaggtgacacattggtaaatcgaattgagctcaaaggaatagattttaaggaagatggtaacatccttggtcataagttagagtataatgcaatttctgataacgtctacataactgcggataaacagaaaaatggtattaaagccaattttaaaattaggcataacatcgaagatgggagtgttcaacttgcagaccactaccaacaaaatacacccataggagacggtcccgtactgttgccagataaccattatctgtctacacaatctaaattaagcaaagatccaaatgaaaagcgtgaccacatggtgttgctagagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgca
BBa_K165018_sequence
1
cccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccct
BBa_K165054_sequence
1
cccgccgccaccatggagtactagagagaacgtctctcactgaacagtacgctccttctagtatacaacatcagcagcagcacccgcaaagtattagtcctatagcaaatacgcagtatggtgatatccctgttagaccggaaattgatttagatcctagcattgtgcctgtggtccctgaacccactgagcccatcgaaaacaatatatcgcttaatgaggaagtcactttcttcgaaaaggccaagaggtatatcggcaataaacatttatacactgagtttttgaaaattttaaatttgtactctcaagatatacttgatcttgacgatttagtggaaaaggtagatttctacttgggttccaataaagaactatttacgtggttcaaaaactttgttggctaccaagaaaaaaccaaatgtatcgagaatattgttcatgaaaaacatagattggatttagatttatgtgaggcatttggcccaagttacaagaggctaccaaaaagtgacactttcatgccatgctcaggtagggatgatatgtgttgggaagtcttgaacgatgaatgggttggacatcctgtatgggcttccgaagattcgggatttattgctcatcgtaaaaaccagtatgaggaaacactattcaagatcgaagaggaaagacatgagtatgatttttacattgaatcaaatttaagaactattcaatgtttggaaacaattgtaaataagatcgagaacatgactgagaatgaaaaagccaattttaaactgcctccaggtcttggccatacttcaatgactatttataaaaaagtgataaggaaagtttatgataaggaaagggggttcgagattattgatgctttgcatgagcaccctgcggtgacagccccagtcgttctgaaaaggttaaagcaaaaagacgaagaatggagaagagctcaacgtgaatggaataaagtttggagggagttagaacagaaggtttttttcaagtcattagatcatttaggcttaacatttaaacaggctgacaagaaattattaactacaaagcagttgatatcagagattagcagcatcaaagttgatcaaacaaacaaaaaaattcactggttaactcctaaaccaaagagccagttagattttgatttccctgataaaaacattttctatgatatcttgtgtttggctgacacttttataacccataccacagcctattctaatcccgataaagaaagattgaaagatttactaaaatacttcatctctttgtttttttctatttctttcgaaaaaatcgaagaatcgttgtactcccataagcaaaacgtgtcagaatctagcggatctgacgatggcagttctattgcatcaaggaagaggccctatcaacaagaaatgagtttacttgatattttacacaggagcagatatcaaaagctaaagcgttctaatgatgaagatggcaaagttccccagctctctgaaccacccgaagaagaacctaataccattgaggaggaagagctcatcgatgaagaagctaaaaatccgtggctaactgggaatttagtggaggaagcaaactctcagggtattatccaaaatcgcagtatttttaatctattcgctaatacgaatatttacatatttttccgtcattggacaacgatttatgagcggcttttggaaattaagcaaatgaatgaaagggtcacaaaggaaatcaacacaaggtcgacagttacttttgccaaagatctggatttattatcgagtcaactttccgaaatgggactagattttgttggtgaagacgcgtacaaacaggttttaagactgagtagaaggttgattaatggggatcttgaacatcagtggtttgaagagagtttgcggcaagcttacaataacaaagcgtttaaactctatacaattgataaagtcacccaatcgttggtaaagcatgctcataccttgatgactgacgctaaaactgcggaaataatggctttgttcgttaaagatagaaatgcctccaccacgagtgcgaaggaccaaattatctatcgcttgcaggtgcgctcacatatgtccaacacagaaaatatgtttagaatagagtttgataaaagaactctgcatgtttccattcaatatattgcacttgatgatttgacactaaaggaaccaaaggcagacgaagataaatggaaatattatgtaacatcgtatgctctgccacatccaacagaaggcattccgcacgagaaactgaagataccatttttggaaaggctcatcgaatttggacaagatattgatggaacagaggtagatgaggagttttcccccgaaggcatctctgtatcgacattgaaaattaagatccagcctataacctaccaactgcatattgaaaacgggtcttacgatgtttttacccgtaaggctactaacaaatatcctactattgctaatgataatactcaaaaaggaatggtttctcagaagaaggagttgatatcaaaatttctggactgtgcagttggtttgagaaataatttagatgaggctcaaaaattaagtatgcaaaaaaaatgggagaacctaaaagacagcatagcaaagacgagtgctggaaaccagggaatagagagtgaaaccgaaaaaggtaaaattacgaaacaggagcagtcggataatttggacagctctactgcaagtgtactgcctgcatccattactactgtaccacaagatgataatatagaaacgactgggaatactgaatcttcagacaagggggctaagattcaatactagagaagcgtgagcctgaatctgtgtatgaaactgactgccgttgggatggctgtagccaggaatttgactcccaagagcagctggtgcaccacatcaacagcgagcacatccacggggagcggaaggagttcgtgtgccactgggggggctgctccagggagctgaggcccttcaaagcccagtacatgctggtggttcacatgcgcagacacactggcgagaagccacacaagtgcacgtttgaagggtgccggaagtcatactcacgcctcgaaaacctgaagacgcacctgcggtcacacacgggtgagaagccatacatgtgtgagcacgagggctgtagtaaagccttcagcaatgccagtgaccgagccaagcaccagaatcggacccattccaatgagaagccgtatgtatgtaagctccctggctgcaccaaacgctatacagatcctagctcgctgcgaaaacatgtcaagacagtgcatggtcctgacgcccatgtgaccaaacggcaccgtggggattactagaggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattctggttgaattggatggcgatgtcaatggccataagttttcggttagtggtgaaggagagggcgacgctacctatgggaagttaactttaaagttcatttgtactaccggtaagttaccagttccttggcctactttggtcacaacccttacatggggggtgcagtgctttgccagatatccggatcacatgaaacaacacgattttttcaaatccgctatgcctgaaggatatgtacaagaaagaaccatatttttcaaggatgacggcaactacaaaactagagccgaagttaaattcgaaggtgacacattggtaaatcgaattgagctcaaaggaatagattttaaggaagatggtaacatccttggtcataagttagagtataatgcaatttctgataacgtctacataactgcggataaacagaaaaatggtattaaagccaattttaaaattaggcataacatcgaagatgggagtgttcaacttgcagaccactaccaacaaaatacacccataggagacggtcccgtactgttgccagataaccattatctgtctacacaatctaaattaagcaaagatccaaatgaaaagcgtgaccacatggtgttgctagagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcaactagagcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattctggttgaattggatggcgatgtcaatggccataagttttcggttagtggtgaaggagagggcgacgctacctatgggaagttaactttaaagttcatttgtactaccggtaagttaccagttccttggcctactttggtcacaacccttacatggggggtgcagtgctttgccagatatccggatcacatgaaacaacacgattttttcaaatccgctatgcctgaaggatatgtacaagaaagaaccatatttttcaaggatgacggcaactacaaaactagagccgaagttaaattcgaaggtgacacattggtaaatcgaattgagctcaaaggaatagattttaaggaagatggtaacatccttggtcataagttagagtataatgcaatttctgataacgtctacataactgcggataaacagaaaaatggtattaaagccaattttaaaattaggcataacatcgaagatgggagtgttcaacttgcagaccactaccaacaaaatacacccataggagacggtcccgtactgttgccagataaccattatctgtctacacaatctaaattaagcaaagatccaaatgaaaagcgtgaccacatggtgttgctagagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcatactagagcccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccct
BBa_K165007_sequence
1
aagcgtgagcctgaatctgtgtatgaaactgactgccgttgggatggctgtagccaggaatttgactcccaagagcagctggtgcaccacatcaacagcgagcacatccacggggagcggaaggagttcgtgtgccactgggggggctgctccagggagctgaggcccttcaaagcccagtacatgctggtggttcacatgcgcagacacactggcgagaagccacacaagtgcacgtttgaagggtgccggaagtcatactcacgcctcgaaaacctgaagacgcacctgcggtcacacacgggtgagaagccatacatgtgtgagcacgagggctgtagtaaagccttcagcaatgccagtgaccgagccaagcaccagaatcggacccattccaatgagaagccgtatgtatgtaagctccctggctgcaccaaacgctatacagatcctagctcgctgcgaaaacatgtcaagacagtgcatggtcctgacgcccatgtgaccaaacggcaccgtggggat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z