BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K165083 1 BBa_K165083 pMET25 + Untagged Zif268-HIV activator on pRS305 2008-10-29T12:00:00Z 2015-05-08T01:10:57Z - - false false _267_ 0 2512 58 It's complicated false - false John Szymanski component1997161 1 BBa_K165022 component1997159 1 BBa_K165000 component1997160 1 BBa_K165002 annotation1997160 1 BBa_K165002 range1997160 1 396 413 annotation1997159 1 BBa_K165000 range1997159 1 1 387 annotation1997161 1 BBa_K165022 range1997161 1 422 1087 BBa_K165022 1 BBa_K165022 Zif268-HIV synthetic activator (untagged) 2008-10-27T12:00:00Z 2015-05-08T01:10:55Z From David Drubin in the Silver Lab at Harvard. This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the Zif268-HIV DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Zif268-HIV binding sites, activating transcription downstream. false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K165000 1 BBa_K165000 MET 25 Promoter 2008-10-24T11:00:00Z 2015-05-08T01:10:55Z From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin. The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity. false false _267_ 0 2512 58 It's complicated false None. false John Szymanski BBa_K165002_sequence 1 cccgccgccaccatggag BBa_K165022_sequence 1 cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaaactagagacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgactagacccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K165000_sequence 1 agctccggatgcaagggttcgaatcccttagctctcattattttttgctttttctcttgaggtcacatgatcgcaaaatggcaaatggcacgtgaagctgtcgatattggggaactgtggtggttggcaaatgactaattaagttagtcaaggcgccatcctcatgaaaactgtgtaacataataaccgaagtgtcgaaaaggtggcaccttgtccaattgaacacgctcgatgaaaaaaataagatatatataaggttaagtaaagcgtctgttagaaaggaagtttttcctttttcttgctctcttgtcttttcatctactatttccttcgtgtaatacagggtcgtcagatacatagatacaattctattacccccatccatac BBa_K165083_sequence 1 agctccggatgcaagggttcgaatcccttagctctcattattttttgctttttctcttgaggtcacatgatcgcaaaatggcaaatggcacgtgaagctgtcgatattggggaactgtggtggttggcaaatgactaattaagttagtcaaggcgccatcctcatgaaaactgtgtaacataataaccgaagtgtcgaaaaggtggcaccttgtccaattgaacacgctcgatgaaaaaaataagatatatataaggttaagtaaagcgtctgttagaaaggaagtttttcctttttcttgctctcttgtcttttcatctactatttccttcgtgtaatacagggtcgtcagatacatagatacaattctattacccccatccatactactagagcccgccgccaccatggagtactagagcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaaactagagacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgactagacccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z