BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K165037 1 pTEF TEF2 yeast constitutive promoter 2008-10-29T12:00:00Z 2015-05-08T01:10:56Z From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7 Released HQ 2013 This is a high level constitutive promoter from the yeast gene TEF2. false false _267_ 0 2512 58 In stock true - true John Szymanski BBa_K165087 1 BBa_K165087 pTEF2 + Untagged Zif268-HIV activator on pRS305 2008-10-29T12:00:00Z 2015-05-08T01:10:57Z - - false false _267_ 0 2512 58 It's complicated false - false John Szymanski component1997193 1 BBa_K165037 component1997195 1 BBa_K165022 component1997194 1 BBa_K165002 annotation1997195 1 BBa_K165022 range1997195 1 438 1103 annotation1997193 1 BBa_K165037 range1997193 1 1 403 annotation1997194 1 BBa_K165002 range1997194 1 412 429 BBa_K165022 1 BBa_K165022 Zif268-HIV synthetic activator (untagged) 2008-10-27T12:00:00Z 2015-05-08T01:10:55Z From David Drubin in the Silver Lab at Harvard. This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the Zif268-HIV DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Zif268-HIV binding sites, activating transcription downstream. false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K165002_sequence 1 cccgccgccaccatggag BBa_K165022_sequence 1 cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaaactagagacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgactagacccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K165037_sequence 1 attacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaac BBa_K165087_sequence 1 attacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaactactagagcccgccgccaccatggagtactagagcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaaactagagacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgactagacccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z