LOCUS BBa_K165090 5396 bp DNA linear 26-Dec-2024 DEFINITION Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306 ACCESSION BBa_K165090 VERSION BBa_K165090.1 FEATURES Location/Qualifiers promoter 1..127 /label=BBa_K165012 promoter 136..538 /label=BBa_K165031 RBS 547..564 /label=BBa_K165002 misc_feature 573..830 /label=BBa_K165009 misc_feature 839..3649 /label=BBa_K165003 misc_feature 3658..4392 /label=BBa_K165004 misc_feature 4401..5135 /label=BBa_K165004 terminator 5144..5396 /label=BBa_K165018 ORIGIN 1 ctcgagcgaa gaccacccac aatgatggtc gaagaccacc cacaatgatg gtcgaagacc 61 acccacaatg atggtcgaag accacccaca atgatggtcg aagaccaccc acaatgatgg 121 tctcgagtac tagagctgta tataaaacca gtggttatat gtacagacta gactgtatat 181 aaaaccagtg gttatatgta cagactagac tgtatataaa accagtggtt atatgtacag 241 actagactgt atataaaacc agtggttata tgtacagact agactcgagc agatccgcca 301 ggcgtgtata tatagcgtgg atggccaggc aactttagtg ctgacacata caggcatata 361 tatatgtgtg cgacgacaca tgatcatatg gcatgcatgt gctctgtatg tatataaaac 421 tcttgttttc ttcttttctc taaatattct ttccttatac attaggacct ttgcagcata 481 aattactata cttctataga cacacaaaca caaatacaca cactaaatta ataactagta 541 ctagagcccg ccgccaccat ggagtactag agaaagcgtt aacggccagg caacaagagg 601 tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa 661 tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg 721 cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg 781 aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaacca tactagagag 841 aacgtctctc actgaacagt acgctccttc tagtatacaa catcagcagc agcacccgca 901 aagtattagt cctatagcaa atacgcagta tggtgatatc cctgttagac cggaaattga 961 tttagatcct agcattgtgc ctgtggtccc tgaacccact gagcccatcg aaaacaatat 1021 atcgcttaat gaggaagtca ctttcttcga aaaggccaag aggtatatcg gcaataaaca 1081 tttatacact gagtttttga aaattttaaa tttgtactct caagatatac ttgatcttga 1141 cgatttagtg gaaaaggtag atttctactt gggttccaat aaagaactat ttacgtggtt 1201 caaaaacttt gttggctacc aagaaaaaac caaatgtatc gagaatattg ttcatgaaaa 1261 acatagattg gatttagatt tatgtgaggc atttggccca agttacaaga ggctaccaaa 1321 aagtgacact ttcatgccat gctcaggtag ggatgatatg tgttgggaag tcttgaacga 1381 tgaatgggtt ggacatcctg tatgggcttc cgaagattcg ggatttattg ctcatcgtaa 1441 aaaccagtat gaggaaacac tattcaagat cgaagaggaa agacatgagt atgattttta 1501 cattgaatca aatttaagaa ctattcaatg tttggaaaca attgtaaata agatcgagaa 1561 catgactgag aatgaaaaag ccaattttaa actgcctcca ggtcttggcc atacttcaat 1621 gactatttat aaaaaagtga taaggaaagt ttatgataag gaaagggggt tcgagattat 1681 tgatgctttg catgagcacc ctgcggtgac agccccagtc gttctgaaaa ggttaaagca 1741 aaaagacgaa gaatggagaa gagctcaacg tgaatggaat aaagtttgga gggagttaga 1801 acagaaggtt tttttcaagt cattagatca tttaggctta acatttaaac aggctgacaa 1861 gaaattatta actacaaagc agttgatatc agagattagc agcatcaaag ttgatcaaac 1921 aaacaaaaaa attcactggt taactcctaa accaaagagc cagttagatt ttgatttccc 1981 tgataaaaac attttctatg atatcttgtg tttggctgac acttttataa cccataccac 2041 agcctattct aatcccgata aagaaagatt gaaagattta ctaaaatact tcatctcttt 2101 gtttttttct atttctttcg aaaaaatcga agaatcgttg tactcccata agcaaaacgt 2161 gtcagaatct agcggatctg acgatggcag ttctattgca tcaaggaaga ggccctatca 2221 acaagaaatg agtttacttg atattttaca caggagcaga tatcaaaagc taaagcgttc 2281 taatgatgaa gatggcaaag ttccccagct ctctgaacca cccgaagaag aacctaatac 2341 cattgaggag gaagagctca tcgatgaaga agctaaaaat ccgtggctaa ctgggaattt 2401 agtggaggaa gcaaactctc agggtattat ccaaaatcgc agtattttta atctattcgc 2461 taatacgaat atttacatat ttttccgtca ttggacaacg atttatgagc ggcttttgga 2521 aattaagcaa atgaatgaaa gggtcacaaa ggaaatcaac acaaggtcga cagttacttt 2581 tgccaaagat ctggatttat tatcgagtca actttccgaa atgggactag attttgttgg 2641 tgaagacgcg tacaaacagg ttttaagact gagtagaagg ttgattaatg gggatcttga 2701 acatcagtgg tttgaagaga gtttgcggca agcttacaat aacaaagcgt ttaaactcta 2761 tacaattgat aaagtcaccc aatcgttggt aaagcatgct cataccttga tgactgacgc 2821 taaaactgcg gaaataatgg ctttgttcgt taaagataga aatgcctcca ccacgagtgc 2881 gaaggaccaa attatctatc gcttgcaggt gcgctcacat atgtccaaca cagaaaatat 2941 gtttagaata gagtttgata aaagaactct gcatgtttcc attcaatata ttgcacttga 3001 tgatttgaca ctaaaggaac caaaggcaga cgaagataaa tggaaatatt atgtaacatc 3061 gtatgctctg ccacatccaa cagaaggcat tccgcacgag aaactgaaga taccattttt 3121 ggaaaggctc atcgaatttg gacaagatat tgatggaaca gaggtagatg aggagttttc 3181 ccccgaaggc atctctgtat cgacattgaa aattaagatc cagcctataa cctaccaact 3241 gcatattgaa aacgggtctt acgatgtttt tacccgtaag gctactaaca aatatcctac 3301 tattgctaat gataatactc aaaaaggaat ggtttctcag aagaaggagt tgatatcaaa 3361 atttctggac tgtgcagttg gtttgagaaa taatttagat gaggctcaaa aattaagtat 3421 gcaaaaaaaa tgggagaacc taaaagacag catagcaaag acgagtgctg gaaaccaggg 3481 aatagagagt gaaaccgaaa aaggtaaaat tacgaaacag gagcagtcgg ataatttgga 3541 cagctctact gcaagtgtac tgcctgcatc cattactact gtaccacaag atgataatat 3601 agaaacgact gggaatactg aatcttcaga caagggggct aagattcaat actagaggca 3661 actagcggca tggttagtaa aggagaagaa aataacatgg caatcattaa ggagttcatg 3721 agattcaaag ttcacatgga aggttctgta aatggacatg aatttgaaat agaaggtgaa 3781 ggagaaggaa ggccttatga aggaacccaa accgcgaagc taaaagttac taagggtggc 3841 ccattaccat ttgcatggga tatccttagc cctcaattca tgtatgggtc aaaggcttat 3901 gtcaagcacc ccgccgacat tccagactat ctaaagttat cttttcccga agggtttaag 3961 tgggagcgtg tgatgaactt cgaagacggt ggcgtggtaa cagtgactca ggattcgtcc 4021 ctgcaagatg gtgaatttat ctacaaagtc aaattaagag gaactaactt tccatctgac 4081 ggcccggtta tgcaaaaaaa gacaatgggc tgggaggcct cctcagaacg aatgtaccct 4141 gaagatggtg ccttgaaggg tgagattaaa caaagattga aattgaaaga tggtggacat 4201 tatgacgctg aggttaaaac gacatacaaa gctaagaaac ctgtccagct cccaggtgct 4261 tacaatgtaa atataaaact tgatattaca tcacataatg aagattatac gatagttgaa 4321 caatacgaaa gggctgaggg gagacatagt actggtggca tggatgaact atacaaaggt 4381 tctggtaccg catactagag gcaactagcg gcatggttag taaaggagaa gaaaataaca 4441 tggcaatcat taaggagttc atgagattca aagttcacat ggaaggttct gtaaatggac 4501 atgaatttga aatagaaggt gaaggagaag gaaggcctta tgaaggaacc caaaccgcga 4561 agctaaaagt tactaagggt ggcccattac catttgcatg ggatatcctt agccctcaat 4621 tcatgtatgg gtcaaaggct tatgtcaagc accccgccga cattccagac tatctaaagt 4681 tatcttttcc cgaagggttt aagtgggagc gtgtgatgaa cttcgaagac ggtggcgtgg 4741 taacagtgac tcaggattcg tccctgcaag atggtgaatt tatctacaaa gtcaaattaa 4801 gaggaactaa ctttccatct gacggcccgg ttatgcaaaa aaagacaatg ggctgggagg 4861 cctcctcaga acgaatgtac cctgaagatg gtgccttgaa gggtgagatt aaacaaagat 4921 tgaaattgaa agatggtgga cattatgacg ctgaggttaa aacgacatac aaagctaaga 4981 aacctgtcca gctcccaggt gcttacaatg taaatataaa acttgatatt acatcacata 5041 atgaagatta tacgatagtt gaacaatacg aaagggctga ggggagacat agtactggtg 5101 gcatggatga actatacaaa ggttctggta ccgcatacta gagcccaaga aaaagcgcaa 5161 ggtaactaga taataagcga atttcttatg atttatgatt tttattatta aataagttat 5221 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 5281 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc 5341 tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccct //