BBa_K165090 1 BBa_K165090 Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306 2008-10-29T12:00:00Z 2015-05-08T01:10:57Z - - false true _267_ 0 2512 58 It's complicated true - true John Szymanski component1997225 1 BBa_K165009 component1997227 1 BBa_K165004 component1997224 1 BBa_K165002 component1997222 1 BBa_K165012 component1997228 1 BBa_K165004 component1997229 1 BBa_K165018 component1997223 1 BBa_K165031 component1997226 1 BBa_K165003 annotation1997225 1 BBa_K165009 range1997225 1 573 830 annotation1997224 1 BBa_K165002 range1997224 1 547 564 annotation1997222 1 BBa_K165012 range1997222 1 1 127 annotation1997226 1 BBa_K165003 range1997226 1 839 3649 annotation1997223 1 BBa_K165031 range1997223 1 136 538 annotation1997228 1 BBa_K165004 range1997228 1 4401 5135 annotation1997227 1 BBa_K165004 range1997227 1 3658 4392 annotation1997229 1 BBa_K165018 range1997229 1 5144 5396 BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K165009 1 lexA DBD LexA DNA-binding domain 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 - false false _267_ 0 2512 58 In stock true - false John Szymanski BBa_K165012 1 BBa_K165012 Gli1 binding sites 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - - false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K165003 1 Sin3 Sin3 Repression Domain 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z From the yeast genome, via Caroline Ajo-Franklin. This is a transcriptional repression domain. It can be fused to many DNA-binding domains to create synthetic transcriptional repressors. We have used it upstream of DNA binding domains. false false _267_ 0 2512 58 It's complicated true - false John Szymanski BBa_K165004 1 mCherry mCherry, yeast optimized for fusion 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z From Caroline Ajo-Franklin This is the mCherry fluorescent protein, yeast-codon optimized and biobricked in the silver lab BioFusion standard for use in fusion proteins. false false _267_ 0 2512 58 It's complicated true - false John Szymanski BBa_K165031 1 BBa_K165031 mCYC promoter plus LexA binding sites 2008-10-28T12:00:00Z 2015-05-08T01:10:56Z Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory. This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain. false false _267_ 0 2510 58 It's complicated false It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector false Aaron Glieberman BBa_K165018 1 NLS+Term SV40 nuclear localization sequence + ADH1 transcription terminator 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - The SV40 nuclear localization sequence can be added to any coding region to have it expressed in the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences. false false _267_ 0 2512 58 It's complicated true - true John Szymanski BBa_K165090_sequence 1 ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagtactagagctgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactagtactagagcccgccgccaccatggagtactagagaaagcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaaccatactagagagaacgtctctcactgaacagtacgctccttctagtatacaacatcagcagcagcacccgcaaagtattagtcctatagcaaatacgcagtatggtgatatccctgttagaccggaaattgatttagatcctagcattgtgcctgtggtccctgaacccactgagcccatcgaaaacaatatatcgcttaatgaggaagtcactttcttcgaaaaggccaagaggtatatcggcaataaacatttatacactgagtttttgaaaattttaaatttgtactctcaagatatacttgatcttgacgatttagtggaaaaggtagatttctacttgggttccaataaagaactatttacgtggttcaaaaactttgttggctaccaagaaaaaaccaaatgtatcgagaatattgttcatgaaaaacatagattggatttagatttatgtgaggcatttggcccaagttacaagaggctaccaaaaagtgacactttcatgccatgctcaggtagggatgatatgtgttgggaagtcttgaacgatgaatgggttggacatcctgtatgggcttccgaagattcgggatttattgctcatcgtaaaaaccagtatgaggaaacactattcaagatcgaagaggaaagacatgagtatgatttttacattgaatcaaatttaagaactattcaatgtttggaaacaattgtaaataagatcgagaacatgactgagaatgaaaaagccaattttaaactgcctccaggtcttggccatacttcaatgactatttataaaaaagtgataaggaaagtttatgataaggaaagggggttcgagattattgatgctttgcatgagcaccctgcggtgacagccccagtcgttctgaaaaggttaaagcaaaaagacgaagaatggagaagagctcaacgtgaatggaataaagtttggagggagttagaacagaaggtttttttcaagtcattagatcatttaggcttaacatttaaacaggctgacaagaaattattaactacaaagcagttgatatcagagattagcagcatcaaagttgatcaaacaaacaaaaaaattcactggttaactcctaaaccaaagagccagttagattttgatttccctgataaaaacattttctatgatatcttgtgtttggctgacacttttataacccataccacagcctattctaatcccgataaagaaagattgaaagatttactaaaatacttcatctctttgtttttttctatttctttcgaaaaaatcgaagaatcgttgtactcccataagcaaaacgtgtcagaatctagcggatctgacgatggcagttctattgcatcaaggaagaggccctatcaacaagaaatgagtttacttgatattttacacaggagcagatatcaaaagctaaagcgttctaatgatgaagatggcaaagttccccagctctctgaaccacccgaagaagaacctaataccattgaggaggaagagctcatcgatgaagaagctaaaaatccgtggctaactgggaatttagtggaggaagcaaactctcagggtattatccaaaatcgcagtatttttaatctattcgctaatacgaatatttacatatttttccgtcattggacaacgatttatgagcggcttttggaaattaagcaaatgaatgaaagggtcacaaaggaaatcaacacaaggtcgacagttacttttgccaaagatctggatttattatcgagtcaactttccgaaatgggactagattttgttggtgaagacgcgtacaaacaggttttaagactgagtagaaggttgattaatggggatcttgaacatcagtggtttgaagagagtttgcggcaagcttacaataacaaagcgtttaaactctatacaattgataaagtcacccaatcgttggtaaagcatgctcataccttgatgactgacgctaaaactgcggaaataatggctttgttcgttaaagatagaaatgcctccaccacgagtgcgaaggaccaaattatctatcgcttgcaggtgcgctcacatatgtccaacacagaaaatatgtttagaatagagtttgataaaagaactctgcatgtttccattcaatatattgcacttgatgatttgacactaaaggaaccaaaggcagacgaagataaatggaaatattatgtaacatcgtatgctctgccacatccaacagaaggcattccgcacgagaaactgaagataccatttttggaaaggctcatcgaatttggacaagatattgatggaacagaggtagatgaggagttttcccccgaaggcatctctgtatcgacattgaaaattaagatccagcctataacctaccaactgcatattgaaaacgggtcttacgatgtttttacccgtaaggctactaacaaatatcctactattgctaatgataatactcaaaaaggaatggtttctcagaagaaggagttgatatcaaaatttctggactgtgcagttggtttgagaaataatttagatgaggctcaaaaattaagtatgcaaaaaaaatgggagaacctaaaagacagcatagcaaagacgagtgctggaaaccagggaatagagagtgaaaccgaaaaaggtaaaattacgaaacaggagcagtcggataatttggacagctctactgcaagtgtactgcctgcatccattactactgtaccacaagatgataatatagaaacgactgggaatactgaatcttcagacaagggggctaagattcaatactagaggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcatactagaggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcatactagagcccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccct BBa_K165002_sequence 1 cccgccgccaccatggag BBa_K165003_sequence 1 agaacgtctctcactgaacagtacgctccttctagtatacaacatcagcagcagcacccgcaaagtattagtcctatagcaaatacgcagtatggtgatatccctgttagaccggaaattgatttagatcctagcattgtgcctgtggtccctgaacccactgagcccatcgaaaacaatatatcgcttaatgaggaagtcactttcttcgaaaaggccaagaggtatatcggcaataaacatttatacactgagtttttgaaaattttaaatttgtactctcaagatatacttgatcttgacgatttagtggaaaaggtagatttctacttgggttccaataaagaactatttacgtggttcaaaaactttgttggctaccaagaaaaaaccaaatgtatcgagaatattgttcatgaaaaacatagattggatttagatttatgtgaggcatttggcccaagttacaagaggctaccaaaaagtgacactttcatgccatgctcaggtagggatgatatgtgttgggaagtcttgaacgatgaatgggttggacatcctgtatgggcttccgaagattcgggatttattgctcatcgtaaaaaccagtatgaggaaacactattcaagatcgaagaggaaagacatgagtatgatttttacattgaatcaaatttaagaactattcaatgtttggaaacaattgtaaataagatcgagaacatgactgagaatgaaaaagccaattttaaactgcctccaggtcttggccatacttcaatgactatttataaaaaagtgataaggaaagtttatgataaggaaagggggttcgagattattgatgctttgcatgagcaccctgcggtgacagccccagtcgttctgaaaaggttaaagcaaaaagacgaagaatggagaagagctcaacgtgaatggaataaagtttggagggagttagaacagaaggtttttttcaagtcattagatcatttaggcttaacatttaaacaggctgacaagaaattattaactacaaagcagttgatatcagagattagcagcatcaaagttgatcaaacaaacaaaaaaattcactggttaactcctaaaccaaagagccagttagattttgatttccctgataaaaacattttctatgatatcttgtgtttggctgacacttttataacccataccacagcctattctaatcccgataaagaaagattgaaagatttactaaaatacttcatctctttgtttttttctatttctttcgaaaaaatcgaagaatcgttgtactcccataagcaaaacgtgtcagaatctagcggatctgacgatggcagttctattgcatcaaggaagaggccctatcaacaagaaatgagtttacttgatattttacacaggagcagatatcaaaagctaaagcgttctaatgatgaagatggcaaagttccccagctctctgaaccacccgaagaagaacctaataccattgaggaggaagagctcatcgatgaagaagctaaaaatccgtggctaactgggaatttagtggaggaagcaaactctcagggtattatccaaaatcgcagtatttttaatctattcgctaatacgaatatttacatatttttccgtcattggacaacgatttatgagcggcttttggaaattaagcaaatgaatgaaagggtcacaaaggaaatcaacacaaggtcgacagttacttttgccaaagatctggatttattatcgagtcaactttccgaaatgggactagattttgttggtgaagacgcgtacaaacaggttttaagactgagtagaaggttgattaatggggatcttgaacatcagtggtttgaagagagtttgcggcaagcttacaataacaaagcgtttaaactctatacaattgataaagtcacccaatcgttggtaaagcatgctcataccttgatgactgacgctaaaactgcggaaataatggctttgttcgttaaagatagaaatgcctccaccacgagtgcgaaggaccaaattatctatcgcttgcaggtgcgctcacatatgtccaacacagaaaatatgtttagaatagagtttgataaaagaactctgcatgtttccattcaatatattgcacttgatgatttgacactaaaggaaccaaaggcagacgaagataaatggaaatattatgtaacatcgtatgctctgccacatccaacagaaggcattccgcacgagaaactgaagataccatttttggaaaggctcatcgaatttggacaagatattgatggaacagaggtagatgaggagttttcccccgaaggcatctctgtatcgacattgaaaattaagatccagcctataacctaccaactgcatattgaaaacgggtcttacgatgtttttacccgtaaggctactaacaaatatcctactattgctaatgataatactcaaaaaggaatggtttctcagaagaaggagttgatatcaaaatttctggactgtgcagttggtttgagaaataatttagatgaggctcaaaaattaagtatgcaaaaaaaatgggagaacctaaaagacagcatagcaaagacgagtgctggaaaccagggaatagagagtgaaaccgaaaaaggtaaaattacgaaacaggagcagtcggataatttggacagctctactgcaagtgtactgcctgcatccattactactgtaccacaagatgataatatagaaacgactgggaatactgaatcttcagacaagggggctaagattcaa BBa_K165031_sequence 1 ctgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag BBa_K165018_sequence 1 cccaagaaaaagcgcaaggtaactagataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccct BBa_K165009_sequence 1 aaagcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaacca BBa_K165012_sequence 1 ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgag BBa_K165004_sequence 1 gcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z