LOCUS BBa_K165091 5309 bp DNA linear 26-Dec-2024 DEFINITION Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS306 ACCESSION BBa_K165091 VERSION BBa_K165091.1 FEATURES Location/Qualifiers promoter 1..46 /label=BBa_K165011 promoter 55..457 /label=BBa_K165031 RBS 466..483 /label=BBa_K165002 misc_feature 492..3302 /label=BBa_K165003 misc_feature 3311..3562 /label=BBa_K165006 misc_feature 3571..4305 /label=BBa_K165004 misc_feature 4314..5048 /label=BBa_K165004 terminator 5057..5309 /label=BBa_K165018 ORIGIN 1 ctcgagcgat gctgcatcga tgctgcatcg atgctgcatt ctcgagtact agagctgtat 61 ataaaaccag tggttatatg tacagactag actgtatata aaaccagtgg ttatatgtac 121 agactagact gtatataaaa ccagtggtta tatgtacaga ctagactgta tataaaacca 181 gtggttatat gtacagacta gactcgagca gatccgccag gcgtgtatat atagcgtgga 241 tggccaggca actttagtgc tgacacatac aggcatatat atatgtgtgc gacgacacat 301 gatcatatgg catgcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct 361 aaatattctt tccttataca ttaggacctt tgcagcataa attactatac ttctatagac 421 acacaaacac aaatacacac actaaattaa taactagtac tagagcccgc cgccaccatg 481 gagtactaga gagaacgtct ctcactgaac agtacgctcc ttctagtata caacatcagc 541 agcagcaccc gcaaagtatt agtcctatag caaatacgca gtatggtgat atccctgtta 601 gaccggaaat tgatttagat cctagcattg tgcctgtggt ccctgaaccc actgagccca 661 tcgaaaacaa tatatcgctt aatgaggaag tcactttctt cgaaaaggcc aagaggtata 721 tcggcaataa acatttatac actgagtttt tgaaaatttt aaatttgtac tctcaagata 781 tacttgatct tgacgattta gtggaaaagg tagatttcta cttgggttcc aataaagaac 841 tatttacgtg gttcaaaaac tttgttggct accaagaaaa aaccaaatgt atcgagaata 901 ttgttcatga aaaacataga ttggatttag atttatgtga ggcatttggc ccaagttaca 961 agaggctacc aaaaagtgac actttcatgc catgctcagg tagggatgat atgtgttggg 1021 aagtcttgaa cgatgaatgg gttggacatc ctgtatgggc ttccgaagat tcgggattta 1081 ttgctcatcg taaaaaccag tatgaggaaa cactattcaa gatcgaagag gaaagacatg 1141 agtatgattt ttacattgaa tcaaatttaa gaactattca atgtttggaa acaattgtaa 1201 ataagatcga gaacatgact gagaatgaaa aagccaattt taaactgcct ccaggtcttg 1261 gccatacttc aatgactatt tataaaaaag tgataaggaa agtttatgat aaggaaaggg 1321 ggttcgagat tattgatgct ttgcatgagc accctgcggt gacagcccca gtcgttctga 1381 aaaggttaaa gcaaaaagac gaagaatgga gaagagctca acgtgaatgg aataaagttt 1441 ggagggagtt agaacagaag gtttttttca agtcattaga tcatttaggc ttaacattta 1501 aacaggctga caagaaatta ttaactacaa agcagttgat atcagagatt agcagcatca 1561 aagttgatca aacaaacaaa aaaattcact ggttaactcc taaaccaaag agccagttag 1621 attttgattt ccctgataaa aacattttct atgatatctt gtgtttggct gacactttta 1681 taacccatac cacagcctat tctaatcccg ataaagaaag attgaaagat ttactaaaat 1741 acttcatctc tttgtttttt tctatttctt tcgaaaaaat cgaagaatcg ttgtactccc 1801 ataagcaaaa cgtgtcagaa tctagcggat ctgacgatgg cagttctatt gcatcaagga 1861 agaggcccta tcaacaagaa atgagtttac ttgatatttt acacaggagc agatatcaaa 1921 agctaaagcg ttctaatgat gaagatggca aagttcccca gctctctgaa ccacccgaag 1981 aagaacctaa taccattgag gaggaagagc tcatcgatga agaagctaaa aatccgtggc 2041 taactgggaa tttagtggag gaagcaaact ctcagggtat tatccaaaat cgcagtattt 2101 ttaatctatt cgctaatacg aatatttaca tatttttccg tcattggaca acgatttatg 2161 agcggctttt ggaaattaag caaatgaatg aaagggtcac aaaggaaatc aacacaaggt 2221 cgacagttac ttttgccaaa gatctggatt tattatcgag tcaactttcc gaaatgggac 2281 tagattttgt tggtgaagac gcgtacaaac aggttttaag actgagtaga aggttgatta 2341 atggggatct tgaacatcag tggtttgaag agagtttgcg gcaagcttac aataacaaag 2401 cgtttaaact ctatacaatt gataaagtca cccaatcgtt ggtaaagcat gctcatacct 2461 tgatgactga cgctaaaact gcggaaataa tggctttgtt cgttaaagat agaaatgcct 2521 ccaccacgag tgcgaaggac caaattatct atcgcttgca ggtgcgctca catatgtcca 2581 acacagaaaa tatgtttaga atagagtttg ataaaagaac tctgcatgtt tccattcaat 2641 atattgcact tgatgatttg acactaaagg aaccaaaggc agacgaagat aaatggaaat 2701 attatgtaac atcgtatgct ctgccacatc caacagaagg cattccgcac gagaaactga 2761 agataccatt tttggaaagg ctcatcgaat ttggacaaga tattgatgga acagaggtag 2821 atgaggagtt ttcccccgaa ggcatctctg tatcgacatt gaaaattaag atccagccta 2881 taacctacca actgcatatt gaaaacgggt cttacgatgt ttttacccgt aaggctacta 2941 acaaatatcc tactattgct aatgataata ctcaaaaagg aatggtttct cagaagaagg 3001 agttgatatc aaaatttctg gactgtgcag ttggtttgag aaataattta gatgaggctc 3061 aaaaattaag tatgcaaaaa aaatgggaga acctaaaaga cagcatagca aagacgagtg 3121 ctggaaacca gggaatagag agtgaaaccg aaaaaggtaa aattacgaaa caggagcagt 3181 cggataattt ggacagctct actgcaagtg tactgcctgc atccattact actgtaccac 3241 aagatgataa tatagaaacg actgggaata ctgaatcttc agacaagggg gctaagattc 3301 aatactagag cccttccagt gtcgcatttg catgcggaac ttttcgctca ggacggacct 3361 tgacaggcat acccgtactc ataccggtga aaaaccgttt cagtgtcgga tctgtatgcg 3421 aaatttctcc ctcagccaga ccttgcgccg ccatctacgt acgcacaccg gcgagaagcc 3481 attccaatgc cgaatatgca tgcgcaactt cagtctcagg agcaacctgg ggcggcacct 3541 aaaaacccac acaggagaaa aatactagag gcaactagcg gcatggttag taaaggagaa 3601 gaaaataaca tggcaatcat taaggagttc atgagattca aagttcacat ggaaggttct 3661 gtaaatggac atgaatttga aatagaaggt gaaggagaag gaaggcctta tgaaggaacc 3721 caaaccgcga agctaaaagt tactaagggt ggcccattac catttgcatg ggatatcctt 3781 agccctcaat tcatgtatgg gtcaaaggct tatgtcaagc accccgccga cattccagac 3841 tatctaaagt tatcttttcc cgaagggttt aagtgggagc gtgtgatgaa cttcgaagac 3901 ggtggcgtgg taacagtgac tcaggattcg tccctgcaag atggtgaatt tatctacaaa 3961 gtcaaattaa gaggaactaa ctttccatct gacggcccgg ttatgcaaaa aaagacaatg 4021 ggctgggagg cctcctcaga acgaatgtac cctgaagatg gtgccttgaa gggtgagatt 4081 aaacaaagat tgaaattgaa agatggtgga cattatgacg ctgaggttaa aacgacatac 4141 aaagctaaga aacctgtcca gctcccaggt gcttacaatg taaatataaa acttgatatt 4201 acatcacata atgaagatta tacgatagtt gaacaatacg aaagggctga ggggagacat 4261 agtactggtg gcatggatga actatacaaa ggttctggta ccgcatacta gaggcaacta 4321 gcggcatggt tagtaaagga gaagaaaata acatggcaat cattaaggag ttcatgagat 4381 tcaaagttca catggaaggt tctgtaaatg gacatgaatt tgaaatagaa ggtgaaggag 4441 aaggaaggcc ttatgaagga acccaaaccg cgaagctaaa agttactaag ggtggcccat 4501 taccatttgc atgggatatc cttagccctc aattcatgta tgggtcaaag gcttatgtca 4561 agcaccccgc cgacattcca gactatctaa agttatcttt tcccgaaggg tttaagtggg 4621 agcgtgtgat gaacttcgaa gacggtggcg tggtaacagt gactcaggat tcgtccctgc 4681 aagatggtga atttatctac aaagtcaaat taagaggaac taactttcca tctgacggcc 4741 cggttatgca aaaaaagaca atgggctggg aggcctcctc agaacgaatg taccctgaag 4801 atggtgcctt gaagggtgag attaaacaaa gattgaaatt gaaagatggt ggacattatg 4861 acgctgaggt taaaacgaca tacaaagcta agaaacctgt ccagctccca ggtgcttaca 4921 atgtaaatat aaaacttgat attacatcac ataatgaaga ttatacgata gttgaacaat 4981 acgaaagggc tgaggggaga catagtactg gtggcatgga tgaactatac aaaggttctg 5041 gtaccgcata ctagagccca agaaaaagcg caaggtaact agataataag cgaatttctt 5101 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta 5161 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt 5221 caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat 5281 gccgagcaaa tgcctgcaaa tcgctccct //