LOCUS BBa_K165093 5598 bp DNA linear 22-Nov-2024 DEFINITION Zif268-HIV bs + TEF2 + Gli1 repressor (CFPx2 tagged) on pRS304* ACCESSION BBa_K165093 VERSION BBa_K165093.1 FEATURES Location/Qualifiers promoter 1..46 /label=BBa_K165011 promoter 55..457 /label=BBa_K165037 RBS 466..483 /label=BBa_K165002 misc_feature 492..3302 /label=BBa_K165003 misc_feature 3311..3835 /label=BBa_K165007 misc_feature 3844..5337 /label=BBa_K165014 terminator 5346..5598 /label=BBa_K165018 ORIGIN 1 ctcgagcgat gctgcatcga tgctgcatcg atgctgcatt ctcgagtact agagattacc 61 cataaggttg tttgtgacgg cgtcgtacaa gagaacgtgg gaacttttta ggctcaccaa 121 aaaagaaaga aaaaatacga gttgctgaca gaagcctcaa gaaaaaaaaa attcttcttc 181 gactatgctg gaggcagaga tgatcgagcc ggtagttaac tatatatagc taaattggtt 241 ccatcacctt cttttctggt gtcgctcctt ctagtgctat ttctggcttt tcctattttt 301 ttttttccat ttttctttct ctctttctaa tatataaatt ctcttgcatt ttctattttt 361 ctctctatct attctacttg tttattccct tcaaggtttt tttttaagga gtacttgttt 421 ttagaatata cggtcaacga actataatta actaaactac tagagcccgc cgccaccatg 481 gagtactaga gagaacgtct ctcactgaac agtacgctcc ttctagtata caacatcagc 541 agcagcaccc gcaaagtatt agtcctatag caaatacgca gtatggtgat atccctgtta 601 gaccggaaat tgatttagat cctagcattg tgcctgtggt ccctgaaccc actgagccca 661 tcgaaaacaa tatatcgctt aatgaggaag tcactttctt cgaaaaggcc aagaggtata 721 tcggcaataa acatttatac actgagtttt tgaaaatttt aaatttgtac tctcaagata 781 tacttgatct tgacgattta gtggaaaagg tagatttcta cttgggttcc aataaagaac 841 tatttacgtg gttcaaaaac tttgttggct accaagaaaa aaccaaatgt atcgagaata 901 ttgttcatga aaaacataga ttggatttag atttatgtga ggcatttggc ccaagttaca 961 agaggctacc aaaaagtgac actttcatgc catgctcagg tagggatgat atgtgttggg 1021 aagtcttgaa cgatgaatgg gttggacatc ctgtatgggc ttccgaagat tcgggattta 1081 ttgctcatcg taaaaaccag tatgaggaaa cactattcaa gatcgaagag gaaagacatg 1141 agtatgattt ttacattgaa tcaaatttaa gaactattca atgtttggaa acaattgtaa 1201 ataagatcga gaacatgact gagaatgaaa aagccaattt taaactgcct ccaggtcttg 1261 gccatacttc aatgactatt tataaaaaag tgataaggaa agtttatgat aaggaaaggg 1321 ggttcgagat tattgatgct ttgcatgagc accctgcggt gacagcccca gtcgttctga 1381 aaaggttaaa gcaaaaagac gaagaatgga gaagagctca acgtgaatgg aataaagttt 1441 ggagggagtt agaacagaag gtttttttca agtcattaga tcatttaggc ttaacattta 1501 aacaggctga caagaaatta ttaactacaa agcagttgat atcagagatt agcagcatca 1561 aagttgatca aacaaacaaa aaaattcact ggttaactcc taaaccaaag agccagttag 1621 attttgattt ccctgataaa aacattttct atgatatctt gtgtttggct gacactttta 1681 taacccatac cacagcctat tctaatcccg ataaagaaag attgaaagat ttactaaaat 1741 acttcatctc tttgtttttt tctatttctt tcgaaaaaat cgaagaatcg ttgtactccc 1801 ataagcaaaa cgtgtcagaa tctagcggat ctgacgatgg cagttctatt gcatcaagga 1861 agaggcccta tcaacaagaa atgagtttac ttgatatttt acacaggagc agatatcaaa 1921 agctaaagcg ttctaatgat gaagatggca aagttcccca gctctctgaa ccacccgaag 1981 aagaacctaa taccattgag gaggaagagc tcatcgatga agaagctaaa aatccgtggc 2041 taactgggaa tttagtggag gaagcaaact ctcagggtat tatccaaaat cgcagtattt 2101 ttaatctatt cgctaatacg aatatttaca tatttttccg tcattggaca acgatttatg 2161 agcggctttt ggaaattaag caaatgaatg aaagggtcac aaaggaaatc aacacaaggt 2221 cgacagttac ttttgccaaa gatctggatt tattatcgag tcaactttcc gaaatgggac 2281 tagattttgt tggtgaagac gcgtacaaac aggttttaag actgagtaga aggttgatta 2341 atggggatct tgaacatcag tggtttgaag agagtttgcg gcaagcttac aataacaaag 2401 cgtttaaact ctatacaatt gataaagtca cccaatcgtt ggtaaagcat gctcatacct 2461 tgatgactga cgctaaaact gcggaaataa tggctttgtt cgttaaagat agaaatgcct 2521 ccaccacgag tgcgaaggac caaattatct atcgcttgca ggtgcgctca catatgtcca 2581 acacagaaaa tatgtttaga atagagtttg ataaaagaac tctgcatgtt tccattcaat 2641 atattgcact tgatgatttg acactaaagg aaccaaaggc agacgaagat aaatggaaat 2701 attatgtaac atcgtatgct ctgccacatc caacagaagg cattccgcac gagaaactga 2761 agataccatt tttggaaagg ctcatcgaat ttggacaaga tattgatgga acagaggtag 2821 atgaggagtt ttcccccgaa ggcatctctg tatcgacatt gaaaattaag atccagccta 2881 taacctacca actgcatatt gaaaacgggt cttacgatgt ttttacccgt aaggctacta 2941 acaaatatcc tactattgct aatgataata ctcaaaaagg aatggtttct cagaagaagg 3001 agttgatatc aaaatttctg gactgtgcag ttggtttgag aaataattta gatgaggctc 3061 aaaaattaag tatgcaaaaa aaatgggaga acctaaaaga cagcatagca aagacgagtg 3121 ctggaaacca gggaatagag agtgaaaccg aaaaaggtaa aattacgaaa caggagcagt 3181 cggataattt ggacagctct actgcaagtg tactgcctgc atccattact actgtaccac 3241 aagatgataa tatagaaacg actgggaata ctgaatcttc agacaagggg gctaagattc 3301 aatactagag aagcgtgagc ctgaatctgt gtatgaaact gactgccgtt gggatggctg 3361 tagccaggaa tttgactccc aagagcagct ggtgcaccac atcaacagcg agcacatcca 3421 cggggagcgg aaggagttcg tgtgccactg ggggggctgc tccagggagc tgaggccctt 3481 caaagcccag tacatgctgg tggttcacat gcgcagacac actggcgaga agccacacaa 3541 gtgcacgttt gaagggtgcc ggaagtcata ctcacgcctc gaaaacctga agacgcacct 3601 gcggtcacac acgggtgaga agccatacat gtgtgagcac gagggctgta gtaaagcctt 3661 cagcaatgcc agtgaccgag ccaagcacca gaatcggacc cattccaatg agaagccgta 3721 tgtatgtaag ctccctggct gcaccaaacg ctatacagat cctagctcgc tgcgaaaaca 3781 tgtcaagaca gtgcatggtc ctgacgccca tgtgaccaaa cggcaccgtg gggattacta 3841 gaggcaacta gcggcatggt tagtaaagga gaagaacttt tcactggagt tgtcccaatt 3901 ctggttgaat tggatggcga tgtcaatggc cataagtttt cggttagtgg tgaaggagag 3961 ggcgacgcta cctatgggaa gttaacttta aagttcattt gtactaccgg taagttacca 4021 gttccttggc ctactttggt cacaaccctt acatgggggg tgcagtgctt tgccagatat 4081 ccggatcaca tgaaacaaca cgattttttc aaatccgcta tgcctgaagg atatgtacaa 4141 gaaagaacca tatttttcaa ggatgacggc aactacaaaa ctagagccga agttaaattc 4201 gaaggtgaca cattggtaaa tcgaattgag ctcaaaggaa tagattttaa ggaagatggt 4261 aacatccttg gtcataagtt agagtataat gcaatttctg ataacgtcta cataactgcg 4321 gataaacaga aaaatggtat taaagccaat tttaaaatta ggcataacat cgaagatggg 4381 agtgttcaac ttgcagacca ctaccaacaa aatacaccca taggagacgg tcccgtactg 4441 ttgccagata accattatct gtctacacaa tctaaattaa gcaaagatcc aaatgaaaag 4501 cgtgaccaca tggtgttgct agagtttgta acagctgctg ggattacaca tggcatggat 4561 gaactataca aaggttctgg taccgcaact agagcaacta gcggcatggt tagtaaagga 4621 gaagaacttt tcactggagt tgtcccaatt ctggttgaat tggatggcga tgtcaatggc 4681 cataagtttt cggttagtgg tgaaggagag ggcgacgcta cctatgggaa gttaacttta 4741 aagttcattt gtactaccgg taagttacca gttccttggc ctactttggt cacaaccctt 4801 acatgggggg tgcagtgctt tgccagatat ccggatcaca tgaaacaaca cgattttttc 4861 aaatccgcta tgcctgaagg atatgtacaa gaaagaacca tatttttcaa ggatgacggc 4921 aactacaaaa ctagagccga agttaaattc gaaggtgaca cattggtaaa tcgaattgag 4981 ctcaaaggaa tagattttaa ggaagatggt aacatccttg gtcataagtt agagtataat 5041 gcaatttctg ataacgtcta cataactgcg gataaacaga aaaatggtat taaagccaat 5101 tttaaaatta ggcataacat cgaagatggg agtgttcaac ttgcagacca ctaccaacaa 5161 aatacaccca taggagacgg tcccgtactg ttgccagata accattatct gtctacacaa 5221 tctaaattaa gcaaagatcc aaatgaaaag cgtgaccaca tggtgttgct agagtttgta 5281 acagctgctg ggattacaca tggcatggat gaactataca aaggttctgg taccgcatac 5341 tagagcccaa gaaaaagcgc aaggtaacta gataataagc gaatttctta tgatttatga 5401 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt 5461 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt 5521 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg ccgagcaaat 5581 gcctgcaaat cgctccct //