LOCUS BBa_K165094 5412 bp DNA linear 22-Nov-2024 DEFINITION Gli1 bs + TEF2 + LexA repressor (CFPx2 tagged) on pRS304* ACCESSION BBa_K165094 VERSION BBa_K165094.1 FEATURES Location/Qualifiers promoter 1..127 /label=BBa_K165012 promoter 136..538 /label=BBa_K165037 RBS 547..564 /label=BBa_K165002 misc_feature 573..830 /label=BBa_K165009 misc_feature 839..3649 /label=BBa_K165003 misc_feature 3658..5151 /label=BBa_K165014 terminator 5160..5412 /label=BBa_K165018 ORIGIN 1 ctcgagcgaa gaccacccac aatgatggtc gaagaccacc cacaatgatg gtcgaagacc 61 acccacaatg atggtcgaag accacccaca atgatggtcg aagaccaccc acaatgatgg 121 tctcgagtac tagagattac ccataaggtt gtttgtgacg gcgtcgtaca agagaacgtg 181 ggaacttttt aggctcacca aaaaagaaag aaaaaatacg agttgctgac agaagcctca 241 agaaaaaaaa aattcttctt cgactatgct ggaggcagag atgatcgagc cggtagttaa 301 ctatatatag ctaaattggt tccatcacct tcttttctgg tgtcgctcct tctagtgcta 361 tttctggctt ttcctatttt tttttttcca tttttctttc tctctttcta atatataaat 421 tctcttgcat tttctatttt tctctctatc tattctactt gtttattccc ttcaaggttt 481 ttttttaagg agtacttgtt tttagaatat acggtcaacg aactataatt aactaaacta 541 ctagagcccg ccgccaccat ggagtactag agaaagcgtt aacggccagg caacaagagg 601 tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa 661 tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg 721 cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg 781 aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaacca tactagagag 841 aacgtctctc actgaacagt acgctccttc tagtatacaa catcagcagc agcacccgca 901 aagtattagt cctatagcaa atacgcagta tggtgatatc cctgttagac cggaaattga 961 tttagatcct agcattgtgc ctgtggtccc tgaacccact gagcccatcg aaaacaatat 1021 atcgcttaat gaggaagtca ctttcttcga aaaggccaag aggtatatcg gcaataaaca 1081 tttatacact gagtttttga aaattttaaa tttgtactct caagatatac ttgatcttga 1141 cgatttagtg gaaaaggtag atttctactt gggttccaat aaagaactat ttacgtggtt 1201 caaaaacttt gttggctacc aagaaaaaac caaatgtatc gagaatattg ttcatgaaaa 1261 acatagattg gatttagatt tatgtgaggc atttggccca agttacaaga ggctaccaaa 1321 aagtgacact ttcatgccat gctcaggtag ggatgatatg tgttgggaag tcttgaacga 1381 tgaatgggtt ggacatcctg tatgggcttc cgaagattcg ggatttattg ctcatcgtaa 1441 aaaccagtat gaggaaacac tattcaagat cgaagaggaa agacatgagt atgattttta 1501 cattgaatca aatttaagaa ctattcaatg tttggaaaca attgtaaata agatcgagaa 1561 catgactgag aatgaaaaag ccaattttaa actgcctcca ggtcttggcc atacttcaat 1621 gactatttat aaaaaagtga taaggaaagt ttatgataag gaaagggggt tcgagattat 1681 tgatgctttg catgagcacc ctgcggtgac agccccagtc gttctgaaaa ggttaaagca 1741 aaaagacgaa gaatggagaa gagctcaacg tgaatggaat aaagtttgga gggagttaga 1801 acagaaggtt tttttcaagt cattagatca tttaggctta acatttaaac aggctgacaa 1861 gaaattatta actacaaagc agttgatatc agagattagc agcatcaaag ttgatcaaac 1921 aaacaaaaaa attcactggt taactcctaa accaaagagc cagttagatt ttgatttccc 1981 tgataaaaac attttctatg atatcttgtg tttggctgac acttttataa cccataccac 2041 agcctattct aatcccgata aagaaagatt gaaagattta ctaaaatact tcatctcttt 2101 gtttttttct atttctttcg aaaaaatcga agaatcgttg tactcccata agcaaaacgt 2161 gtcagaatct agcggatctg acgatggcag ttctattgca tcaaggaaga ggccctatca 2221 acaagaaatg agtttacttg atattttaca caggagcaga tatcaaaagc taaagcgttc 2281 taatgatgaa gatggcaaag ttccccagct ctctgaacca cccgaagaag aacctaatac 2341 cattgaggag gaagagctca tcgatgaaga agctaaaaat ccgtggctaa ctgggaattt 2401 agtggaggaa gcaaactctc agggtattat ccaaaatcgc agtattttta atctattcgc 2461 taatacgaat atttacatat ttttccgtca ttggacaacg atttatgagc ggcttttgga 2521 aattaagcaa atgaatgaaa gggtcacaaa ggaaatcaac acaaggtcga cagttacttt 2581 tgccaaagat ctggatttat tatcgagtca actttccgaa atgggactag attttgttgg 2641 tgaagacgcg tacaaacagg ttttaagact gagtagaagg ttgattaatg gggatcttga 2701 acatcagtgg tttgaagaga gtttgcggca agcttacaat aacaaagcgt ttaaactcta 2761 tacaattgat aaagtcaccc aatcgttggt aaagcatgct cataccttga tgactgacgc 2821 taaaactgcg gaaataatgg ctttgttcgt taaagataga aatgcctcca ccacgagtgc 2881 gaaggaccaa attatctatc gcttgcaggt gcgctcacat atgtccaaca cagaaaatat 2941 gtttagaata gagtttgata aaagaactct gcatgtttcc attcaatata ttgcacttga 3001 tgatttgaca ctaaaggaac caaaggcaga cgaagataaa tggaaatatt atgtaacatc 3061 gtatgctctg ccacatccaa cagaaggcat tccgcacgag aaactgaaga taccattttt 3121 ggaaaggctc atcgaatttg gacaagatat tgatggaaca gaggtagatg aggagttttc 3181 ccccgaaggc atctctgtat cgacattgaa aattaagatc cagcctataa cctaccaact 3241 gcatattgaa aacgggtctt acgatgtttt tacccgtaag gctactaaca aatatcctac 3301 tattgctaat gataatactc aaaaaggaat ggtttctcag aagaaggagt tgatatcaaa 3361 atttctggac tgtgcagttg gtttgagaaa taatttagat gaggctcaaa aattaagtat 3421 gcaaaaaaaa tgggagaacc taaaagacag catagcaaag acgagtgctg gaaaccaggg 3481 aatagagagt gaaaccgaaa aaggtaaaat tacgaaacag gagcagtcgg ataatttgga 3541 cagctctact gcaagtgtac tgcctgcatc cattactact gtaccacaag atgataatat 3601 agaaacgact gggaatactg aatcttcaga caagggggct aagattcaat actagaggca 3661 actagcggca tggttagtaa aggagaagaa cttttcactg gagttgtccc aattctggtt 3721 gaattggatg gcgatgtcaa tggccataag ttttcggtta gtggtgaagg agagggcgac 3781 gctacctatg ggaagttaac tttaaagttc atttgtacta ccggtaagtt accagttcct 3841 tggcctactt tggtcacaac ccttacatgg ggggtgcagt gctttgccag atatccggat 3901 cacatgaaac aacacgattt tttcaaatcc gctatgcctg aaggatatgt acaagaaaga 3961 accatatttt tcaaggatga cggcaactac aaaactagag ccgaagttaa attcgaaggt 4021 gacacattgg taaatcgaat tgagctcaaa ggaatagatt ttaaggaaga tggtaacatc 4081 cttggtcata agttagagta taatgcaatt tctgataacg tctacataac tgcggataaa 4141 cagaaaaatg gtattaaagc caattttaaa attaggcata acatcgaaga tgggagtgtt 4201 caacttgcag accactacca acaaaataca cccataggag acggtcccgt actgttgcca 4261 gataaccatt atctgtctac acaatctaaa ttaagcaaag atccaaatga aaagcgtgac 4321 cacatggtgt tgctagagtt tgtaacagct gctgggatta cacatggcat ggatgaacta 4381 tacaaaggtt ctggtaccgc aactagagca actagcggca tggttagtaa aggagaagaa 4441 cttttcactg gagttgtccc aattctggtt gaattggatg gcgatgtcaa tggccataag 4501 ttttcggtta gtggtgaagg agagggcgac gctacctatg ggaagttaac tttaaagttc 4561 atttgtacta ccggtaagtt accagttcct tggcctactt tggtcacaac ccttacatgg 4621 ggggtgcagt gctttgccag atatccggat cacatgaaac aacacgattt tttcaaatcc 4681 gctatgcctg aaggatatgt acaagaaaga accatatttt tcaaggatga cggcaactac 4741 aaaactagag ccgaagttaa attcgaaggt gacacattgg taaatcgaat tgagctcaaa 4801 ggaatagatt ttaaggaaga tggtaacatc cttggtcata agttagagta taatgcaatt 4861 tctgataacg tctacataac tgcggataaa cagaaaaatg gtattaaagc caattttaaa 4921 attaggcata acatcgaaga tgggagtgtt caacttgcag accactacca acaaaataca 4981 cccataggag acggtcccgt actgttgcca gataaccatt atctgtctac acaatctaaa 5041 ttaagcaaag atccaaatga aaagcgtgac cacatggtgt tgctagagtt tgtaacagct 5101 gctgggatta cacatggcat ggatgaacta tacaaaggtt ctggtaccgc atactagagc 5161 ccaagaaaaa gcgcaaggta actagataat aagcgaattt cttatgattt atgattttta 5221 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt 5281 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg 5341 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc 5401 aaatcgctcc ct //