LOCUS BBa_K165098 6640 bp DNA linear 22-Dec-2024 DEFINITION Zif268-HIV bs + ADH1 + Gli1 repressor (CFPx2 tagged) on pRS304* ACCESSION BBa_K165098 VERSION BBa_K165098.1 FEATURES Location/Qualifiers promoter 1..46 /label=BBa_K165011 promoter 55..1499 /label=BBa_K165015 RBS 1508..1525 /label=BBa_K165002 misc_feature 1534..4344 /label=BBa_K165003 misc_feature 4353..4877 /label=BBa_K165007 misc_feature 4886..6379 /label=BBa_K165014 terminator 6388..6640 /label=BBa_K165018 ORIGIN 1 ctcgagcgat gctgcatcga tgctgcatcg atgctgcatt ctcgagtact agagaagaaa 61 tgatggtaaa tgaaatagga aatcaaggag catgaaggca aaagacaaat ataagggtcg 121 aacgaaaaat aaagtgaaaa gtgttgatat gatgtatttg gctttgcggc gccgaaaaaa 181 cgagtttacg caattgcaca atcatgctga ctctgtggcg gacccgcgct cttgccggcc 241 cggcgataac gctgggcgtg aggctgtgcc cggcggagtt ttttgcgcct gcattttcca 301 aggtttaccc tgcgctaagg ggcgagattg gagaagcaat aagaatgccg gttggggttg 361 cgatgatgac gaccacgaca actggtgtca ttatttaagt tgccgaaaga acctgagtgc 421 atttgcaaca tgagtatact agaagaatga gccaagactt gcgagacgcg agtttgccgg 481 tggtgcgaac aatagagcga ccatgacctt gaaggtgaga cgcgcataac cgctagagta 541 ctttgaagag gaaacagcaa tagggttgct accagtataa atagacaggt acatacaaca 601 ctggaaatgg ttgtctgttt gagtacgctt tcaattcatt tgggtgtgca ctttattatg 661 ttacaatatg gaagggaact ttacacttct cctatgcaca tatattaatt aaagtccaat 721 gctagtagag aaggggggta acacccctcc gcgctctttt ccgatttttt tctaaaccgt 781 ggaatatttc ggatatcctt ttgttgtttc cgggtgtaca atatggactt cctcttttct 841 ggcaaccaaa cccatacatc gggattccta taataccttc gttggtctcc ctaacatgta 901 ggtggcggag gggagatata caatagaaca gataccagac aagacataat gggctaaaca 961 agactacacc aattacactg cctcattgat ggtggtacat aacgaactaa tactgtagcc 1021 ctagacttga tagccatcat catatcgaag tttcactacc ctttttccat ttgccatcta 1081 ttgaagtaat aataggcgca tgcaacttct tttctttttt tttcttttct ctctcccccg 1141 ttgttgtctc accatatccg caatgacaaa aaaaatgatg gaagacacta aaggaaaaaa 1201 ttaacgacaa agacagcacc aacagatgtc gttgttccag agctgatgag gggtatcttc 1261 gaacacacga aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt 1321 atacggcctt ccttccagtt acttgaattt gaaataaaaa aagtttgccg ctttgctatc 1381 aagtataaat agacctgcaa ttattaatct tttgtttcct cgtcattgtt ctcgttccct 1441 ttcttccttg tttctttttc tgcacaatat ttcaagctat accaagcata caatcaactt 1501 actagagccc gccgccacca tggagtacta gagagaacgt ctctcactga acagtacgct 1561 ccttctagta tacaacatca gcagcagcac ccgcaaagta ttagtcctat agcaaatacg 1621 cagtatggtg atatccctgt tagaccggaa attgatttag atcctagcat tgtgcctgtg 1681 gtccctgaac ccactgagcc catcgaaaac aatatatcgc ttaatgagga agtcactttc 1741 ttcgaaaagg ccaagaggta tatcggcaat aaacatttat acactgagtt tttgaaaatt 1801 ttaaatttgt actctcaaga tatacttgat cttgacgatt tagtggaaaa ggtagatttc 1861 tacttgggtt ccaataaaga actatttacg tggttcaaaa actttgttgg ctaccaagaa 1921 aaaaccaaat gtatcgagaa tattgttcat gaaaaacata gattggattt agatttatgt 1981 gaggcatttg gcccaagtta caagaggcta ccaaaaagtg acactttcat gccatgctca 2041 ggtagggatg atatgtgttg ggaagtcttg aacgatgaat gggttggaca tcctgtatgg 2101 gcttccgaag attcgggatt tattgctcat cgtaaaaacc agtatgagga aacactattc 2161 aagatcgaag aggaaagaca tgagtatgat ttttacattg aatcaaattt aagaactatt 2221 caatgtttgg aaacaattgt aaataagatc gagaacatga ctgagaatga aaaagccaat 2281 tttaaactgc ctccaggtct tggccatact tcaatgacta tttataaaaa agtgataagg 2341 aaagtttatg ataaggaaag ggggttcgag attattgatg ctttgcatga gcaccctgcg 2401 gtgacagccc cagtcgttct gaaaaggtta aagcaaaaag acgaagaatg gagaagagct 2461 caacgtgaat ggaataaagt ttggagggag ttagaacaga aggttttttt caagtcatta 2521 gatcatttag gcttaacatt taaacaggct gacaagaaat tattaactac aaagcagttg 2581 atatcagaga ttagcagcat caaagttgat caaacaaaca aaaaaattca ctggttaact 2641 cctaaaccaa agagccagtt agattttgat ttccctgata aaaacatttt ctatgatatc 2701 ttgtgtttgg ctgacacttt tataacccat accacagcct attctaatcc cgataaagaa 2761 agattgaaag atttactaaa atacttcatc tctttgtttt tttctatttc tttcgaaaaa 2821 atcgaagaat cgttgtactc ccataagcaa aacgtgtcag aatctagcgg atctgacgat 2881 ggcagttcta ttgcatcaag gaagaggccc tatcaacaag aaatgagttt acttgatatt 2941 ttacacagga gcagatatca aaagctaaag cgttctaatg atgaagatgg caaagttccc 3001 cagctctctg aaccacccga agaagaacct aataccattg aggaggaaga gctcatcgat 3061 gaagaagcta aaaatccgtg gctaactggg aatttagtgg aggaagcaaa ctctcagggt 3121 attatccaaa atcgcagtat ttttaatcta ttcgctaata cgaatattta catatttttc 3181 cgtcattgga caacgattta tgagcggctt ttggaaatta agcaaatgaa tgaaagggtc 3241 acaaaggaaa tcaacacaag gtcgacagtt acttttgcca aagatctgga tttattatcg 3301 agtcaacttt ccgaaatggg actagatttt gttggtgaag acgcgtacaa acaggtttta 3361 agactgagta gaaggttgat taatggggat cttgaacatc agtggtttga agagagtttg 3421 cggcaagctt acaataacaa agcgtttaaa ctctatacaa ttgataaagt cacccaatcg 3481 ttggtaaagc atgctcatac cttgatgact gacgctaaaa ctgcggaaat aatggctttg 3541 ttcgttaaag atagaaatgc ctccaccacg agtgcgaagg accaaattat ctatcgcttg 3601 caggtgcgct cacatatgtc caacacagaa aatatgttta gaatagagtt tgataaaaga 3661 actctgcatg tttccattca atatattgca cttgatgatt tgacactaaa ggaaccaaag 3721 gcagacgaag ataaatggaa atattatgta acatcgtatg ctctgccaca tccaacagaa 3781 ggcattccgc acgagaaact gaagatacca tttttggaaa ggctcatcga atttggacaa 3841 gatattgatg gaacagaggt agatgaggag ttttcccccg aaggcatctc tgtatcgaca 3901 ttgaaaatta agatccagcc tataacctac caactgcata ttgaaaacgg gtcttacgat 3961 gtttttaccc gtaaggctac taacaaatat cctactattg ctaatgataa tactcaaaaa 4021 ggaatggttt ctcagaagaa ggagttgata tcaaaatttc tggactgtgc agttggtttg 4081 agaaataatt tagatgaggc tcaaaaatta agtatgcaaa aaaaatggga gaacctaaaa 4141 gacagcatag caaagacgag tgctggaaac cagggaatag agagtgaaac cgaaaaaggt 4201 aaaattacga aacaggagca gtcggataat ttggacagct ctactgcaag tgtactgcct 4261 gcatccatta ctactgtacc acaagatgat aatatagaaa cgactgggaa tactgaatct 4321 tcagacaagg gggctaagat tcaatactag agaagcgtga gcctgaatct gtgtatgaaa 4381 ctgactgccg ttgggatggc tgtagccagg aatttgactc ccaagagcag ctggtgcacc 4441 acatcaacag cgagcacatc cacggggagc ggaaggagtt cgtgtgccac tgggggggct 4501 gctccaggga gctgaggccc ttcaaagccc agtacatgct ggtggttcac atgcgcagac 4561 acactggcga gaagccacac aagtgcacgt ttgaagggtg ccggaagtca tactcacgcc 4621 tcgaaaacct gaagacgcac ctgcggtcac acacgggtga gaagccatac atgtgtgagc 4681 acgagggctg tagtaaagcc ttcagcaatg ccagtgaccg agccaagcac cagaatcgga 4741 cccattccaa tgagaagccg tatgtatgta agctccctgg ctgcaccaaa cgctatacag 4801 atcctagctc gctgcgaaaa catgtcaaga cagtgcatgg tcctgacgcc catgtgacca 4861 aacggcaccg tggggattac tagaggcaac tagcggcatg gttagtaaag gagaagaact 4921 tttcactgga gttgtcccaa ttctggttga attggatggc gatgtcaatg gccataagtt 4981 ttcggttagt ggtgaaggag agggcgacgc tacctatggg aagttaactt taaagttcat 5041 ttgtactacc ggtaagttac cagttccttg gcctactttg gtcacaaccc ttacatgggg 5101 ggtgcagtgc tttgccagat atccggatca catgaaacaa cacgattttt tcaaatccgc 5161 tatgcctgaa ggatatgtac aagaaagaac catatttttc aaggatgacg gcaactacaa 5221 aactagagcc gaagttaaat tcgaaggtga cacattggta aatcgaattg agctcaaagg 5281 aatagatttt aaggaagatg gtaacatcct tggtcataag ttagagtata atgcaatttc 5341 tgataacgtc tacataactg cggataaaca gaaaaatggt attaaagcca attttaaaat 5401 taggcataac atcgaagatg ggagtgttca acttgcagac cactaccaac aaaatacacc 5461 cataggagac ggtcccgtac tgttgccaga taaccattat ctgtctacac aatctaaatt 5521 aagcaaagat ccaaatgaaa agcgtgacca catggtgttg ctagagtttg taacagctgc 5581 tgggattaca catggcatgg atgaactata caaaggttct ggtaccgcaa ctagagcaac 5641 tagcggcatg gttagtaaag gagaagaact tttcactgga gttgtcccaa ttctggttga 5701 attggatggc gatgtcaatg gccataagtt ttcggttagt ggtgaaggag agggcgacgc 5761 tacctatggg aagttaactt taaagttcat ttgtactacc ggtaagttac cagttccttg 5821 gcctactttg gtcacaaccc ttacatgggg ggtgcagtgc tttgccagat atccggatca 5881 catgaaacaa cacgattttt tcaaatccgc tatgcctgaa ggatatgtac aagaaagaac 5941 catatttttc aaggatgacg gcaactacaa aactagagcc gaagttaaat tcgaaggtga 6001 cacattggta aatcgaattg agctcaaagg aatagatttt aaggaagatg gtaacatcct 6061 tggtcataag ttagagtata atgcaatttc tgataacgtc tacataactg cggataaaca 6121 gaaaaatggt attaaagcca attttaaaat taggcataac atcgaagatg ggagtgttca 6181 acttgcagac cactaccaac aaaatacacc cataggagac ggtcccgtac tgttgccaga 6241 taaccattat ctgtctacac aatctaaatt aagcaaagat ccaaatgaaa agcgtgacca 6301 catggtgttg ctagagtttg taacagctgc tgggattaca catggcatgg atgaactata 6361 caaaggttct ggtaccgcat actagagccc aagaaaaagc gcaaggtaac tagataataa 6421 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta 6481 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct 6541 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc 6601 tctaccggca tgccgagcaa atgcctgcaa atcgctccct //