LOCUS BBa_K165099 6454 bp DNA linear 22-Dec-2024 DEFINITION Gli1 bs + ADH1+ LexA repressor (CFPx2 tagged) on pRS304* ACCESSION BBa_K165099 VERSION BBa_K165099.1 FEATURES Location/Qualifiers promoter 1..127 /label=BBa_K165012 promoter 136..1580 /label=BBa_K165015 RBS 1589..1606 /label=BBa_K165002 misc_feature 1615..1872 /label=BBa_K165009 misc_feature 1881..4691 /label=BBa_K165003 misc_feature 4700..6193 /label=BBa_K165014 terminator 6202..6454 /label=BBa_K165018 ORIGIN 1 ctcgagcgaa gaccacccac aatgatggtc gaagaccacc cacaatgatg gtcgaagacc 61 acccacaatg atggtcgaag accacccaca atgatggtcg aagaccaccc acaatgatgg 121 tctcgagtac tagagaagaa atgatggtaa atgaaatagg aaatcaagga gcatgaaggc 181 aaaagacaaa tataagggtc gaacgaaaaa taaagtgaaa agtgttgata tgatgtattt 241 ggctttgcgg cgccgaaaaa acgagtttac gcaattgcac aatcatgctg actctgtggc 301 ggacccgcgc tcttgccggc ccggcgataa cgctgggcgt gaggctgtgc ccggcggagt 361 tttttgcgcc tgcattttcc aaggtttacc ctgcgctaag gggcgagatt ggagaagcaa 421 taagaatgcc ggttggggtt gcgatgatga cgaccacgac aactggtgtc attatttaag 481 ttgccgaaag aacctgagtg catttgcaac atgagtatac tagaagaatg agccaagact 541 tgcgagacgc gagtttgccg gtggtgcgaa caatagagcg accatgacct tgaaggtgag 601 acgcgcataa ccgctagagt actttgaaga ggaaacagca atagggttgc taccagtata 661 aatagacagg tacatacaac actggaaatg gttgtctgtt tgagtacgct ttcaattcat 721 ttgggtgtgc actttattat gttacaatat ggaagggaac tttacacttc tcctatgcac 781 atatattaat taaagtccaa tgctagtaga gaaggggggt aacacccctc cgcgctcttt 841 tccgattttt ttctaaaccg tggaatattt cggatatcct tttgttgttt ccgggtgtac 901 aatatggact tcctcttttc tggcaaccaa acccatacat cgggattcct ataatacctt 961 cgttggtctc cctaacatgt aggtggcgga ggggagatat acaatagaac agataccaga 1021 caagacataa tgggctaaac aagactacac caattacact gcctcattga tggtggtaca 1081 taacgaacta atactgtagc cctagacttg atagccatca tcatatcgaa gtttcactac 1141 cctttttcca tttgccatct attgaagtaa taataggcgc atgcaacttc ttttcttttt 1201 ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa aaaaaatgat 1261 ggaagacact aaaggaaaaa attaacgaca aagacagcac caacagatgt cgttgttcca 1321 gagctgatga ggggtatctt cgaacacacg aaactttttc cttccttcat tcacgcacac 1381 tactctctaa tgagcaacgg tatacggcct tccttccagt tacttgaatt tgaaataaaa 1441 aaagtttgcc gctttgctat caagtataaa tagacctgca attattaatc ttttgtttcc 1501 tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata tttcaagcta 1561 taccaagcat acaatcaact tactagagcc cgccgccacc atggagtact agagaaagcg 1621 ttaacggcca ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt 1681 atgccgccga cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct 1741 gaagaacatc tgaaggcgct ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca 1801 cgcgggattc gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct 1861 gccggtgaac catactagag agaacgtctc tcactgaaca gtacgctcct tctagtatac 1921 aacatcagca gcagcacccg caaagtatta gtcctatagc aaatacgcag tatggtgata 1981 tccctgttag accggaaatt gatttagatc ctagcattgt gcctgtggtc cctgaaccca 2041 ctgagcccat cgaaaacaat atatcgctta atgaggaagt cactttcttc gaaaaggcca 2101 agaggtatat cggcaataaa catttataca ctgagttttt gaaaatttta aatttgtact 2161 ctcaagatat acttgatctt gacgatttag tggaaaaggt agatttctac ttgggttcca 2221 ataaagaact atttacgtgg ttcaaaaact ttgttggcta ccaagaaaaa accaaatgta 2281 tcgagaatat tgttcatgaa aaacatagat tggatttaga tttatgtgag gcatttggcc 2341 caagttacaa gaggctacca aaaagtgaca ctttcatgcc atgctcaggt agggatgata 2401 tgtgttggga agtcttgaac gatgaatggg ttggacatcc tgtatgggct tccgaagatt 2461 cgggatttat tgctcatcgt aaaaaccagt atgaggaaac actattcaag atcgaagagg 2521 aaagacatga gtatgatttt tacattgaat caaatttaag aactattcaa tgtttggaaa 2581 caattgtaaa taagatcgag aacatgactg agaatgaaaa agccaatttt aaactgcctc 2641 caggtcttgg ccatacttca atgactattt ataaaaaagt gataaggaaa gtttatgata 2701 aggaaagggg gttcgagatt attgatgctt tgcatgagca ccctgcggtg acagccccag 2761 tcgttctgaa aaggttaaag caaaaagacg aagaatggag aagagctcaa cgtgaatgga 2821 ataaagtttg gagggagtta gaacagaagg tttttttcaa gtcattagat catttaggct 2881 taacatttaa acaggctgac aagaaattat taactacaaa gcagttgata tcagagatta 2941 gcagcatcaa agttgatcaa acaaacaaaa aaattcactg gttaactcct aaaccaaaga 3001 gccagttaga ttttgatttc cctgataaaa acattttcta tgatatcttg tgtttggctg 3061 acacttttat aacccatacc acagcctatt ctaatcccga taaagaaaga ttgaaagatt 3121 tactaaaata cttcatctct ttgttttttt ctatttcttt cgaaaaaatc gaagaatcgt 3181 tgtactccca taagcaaaac gtgtcagaat ctagcggatc tgacgatggc agttctattg 3241 catcaaggaa gaggccctat caacaagaaa tgagtttact tgatatttta cacaggagca 3301 gatatcaaaa gctaaagcgt tctaatgatg aagatggcaa agttccccag ctctctgaac 3361 cacccgaaga agaacctaat accattgagg aggaagagct catcgatgaa gaagctaaaa 3421 atccgtggct aactgggaat ttagtggagg aagcaaactc tcagggtatt atccaaaatc 3481 gcagtatttt taatctattc gctaatacga atatttacat atttttccgt cattggacaa 3541 cgatttatga gcggcttttg gaaattaagc aaatgaatga aagggtcaca aaggaaatca 3601 acacaaggtc gacagttact tttgccaaag atctggattt attatcgagt caactttccg 3661 aaatgggact agattttgtt ggtgaagacg cgtacaaaca ggttttaaga ctgagtagaa 3721 ggttgattaa tggggatctt gaacatcagt ggtttgaaga gagtttgcgg caagcttaca 3781 ataacaaagc gtttaaactc tatacaattg ataaagtcac ccaatcgttg gtaaagcatg 3841 ctcatacctt gatgactgac gctaaaactg cggaaataat ggctttgttc gttaaagata 3901 gaaatgcctc caccacgagt gcgaaggacc aaattatcta tcgcttgcag gtgcgctcac 3961 atatgtccaa cacagaaaat atgtttagaa tagagtttga taaaagaact ctgcatgttt 4021 ccattcaata tattgcactt gatgatttga cactaaagga accaaaggca gacgaagata 4081 aatggaaata ttatgtaaca tcgtatgctc tgccacatcc aacagaaggc attccgcacg 4141 agaaactgaa gataccattt ttggaaaggc tcatcgaatt tggacaagat attgatggaa 4201 cagaggtaga tgaggagttt tcccccgaag gcatctctgt atcgacattg aaaattaaga 4261 tccagcctat aacctaccaa ctgcatattg aaaacgggtc ttacgatgtt tttacccgta 4321 aggctactaa caaatatcct actattgcta atgataatac tcaaaaagga atggtttctc 4381 agaagaagga gttgatatca aaatttctgg actgtgcagt tggtttgaga aataatttag 4441 atgaggctca aaaattaagt atgcaaaaaa aatgggagaa cctaaaagac agcatagcaa 4501 agacgagtgc tggaaaccag ggaatagaga gtgaaaccga aaaaggtaaa attacgaaac 4561 aggagcagtc ggataatttg gacagctcta ctgcaagtgt actgcctgca tccattacta 4621 ctgtaccaca agatgataat atagaaacga ctgggaatac tgaatcttca gacaaggggg 4681 ctaagattca atactagagg caactagcgg catggttagt aaaggagaag aacttttcac 4741 tggagttgtc ccaattctgg ttgaattgga tggcgatgtc aatggccata agttttcggt 4801 tagtggtgaa ggagagggcg acgctaccta tgggaagtta actttaaagt tcatttgtac 4861 taccggtaag ttaccagttc cttggcctac tttggtcaca acccttacat ggggggtgca 4921 gtgctttgcc agatatccgg atcacatgaa acaacacgat tttttcaaat ccgctatgcc 4981 tgaaggatat gtacaagaaa gaaccatatt tttcaaggat gacggcaact acaaaactag 5041 agccgaagtt aaattcgaag gtgacacatt ggtaaatcga attgagctca aaggaataga 5101 ttttaaggaa gatggtaaca tccttggtca taagttagag tataatgcaa tttctgataa 5161 cgtctacata actgcggata aacagaaaaa tggtattaaa gccaatttta aaattaggca 5221 taacatcgaa gatgggagtg ttcaacttgc agaccactac caacaaaata cacccatagg 5281 agacggtccc gtactgttgc cagataacca ttatctgtct acacaatcta aattaagcaa 5341 agatccaaat gaaaagcgtg accacatggt gttgctagag tttgtaacag ctgctgggat 5401 tacacatggc atggatgaac tatacaaagg ttctggtacc gcaactagag caactagcgg 5461 catggttagt aaaggagaag aacttttcac tggagttgtc ccaattctgg ttgaattgga 5521 tggcgatgtc aatggccata agttttcggt tagtggtgaa ggagagggcg acgctaccta 5581 tgggaagtta actttaaagt tcatttgtac taccggtaag ttaccagttc cttggcctac 5641 tttggtcaca acccttacat ggggggtgca gtgctttgcc agatatccgg atcacatgaa 5701 acaacacgat tttttcaaat ccgctatgcc tgaaggatat gtacaagaaa gaaccatatt 5761 tttcaaggat gacggcaact acaaaactag agccgaagtt aaattcgaag gtgacacatt 5821 ggtaaatcga attgagctca aaggaataga ttttaaggaa gatggtaaca tccttggtca 5881 taagttagag tataatgcaa tttctgataa cgtctacata actgcggata aacagaaaaa 5941 tggtattaaa gccaatttta aaattaggca taacatcgaa gatgggagtg ttcaacttgc 6001 agaccactac caacaaaata cacccatagg agacggtccc gtactgttgc cagataacca 6061 ttatctgtct acacaatcta aattaagcaa agatccaaat gaaaagcgtg accacatggt 6121 gttgctagag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaagg 6181 ttctggtacc gcatactaga gcccaagaaa aagcgcaagg taactagata ataagcgaat 6241 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa 6301 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg 6361 taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc 6421 ggcatgccga gcaaatgcct gcaaatcgct ccct //