LOCUS BBa_K1651000 3390 bp DNA linear 29-Dec-2024 DEFINITION PAI1 detector ACCESSION BBa_K1651000 VERSION BBa_K1651000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..784 /label=BBa_C0179 stem_loop 804..847 /label=stem_loop misc_feature 793..872 /label=BBa_B0010 terminator 793..872 /label=BBa_B0010 stem_loop 888..907 /label=T7 TE misc_feature 914..914 /label=stop polyA_site 908..921 /label=polya misc_feature 881..921 /label=BBa_B0012 terminator 881..921 /label=BBa_B0012 terminator 793..921 /label=BBa_B0015 misc_feature 1..921 /label=BBa_K553003 misc_feature 975..992 /label=OP2 misc_feature 1035..1054 /label=OP1 promoter 1046..1051 /label=-35 promoter 1069..1074 /label=-10 promoter 930..1086 /label=BBa_R0079 misc_feature 1099..1102 /label=conserved RBS 1095..1106 /label=BBa_B0034 CDS 1113..1715 /label=rhlL misc_feature 1716..1748 /label=LVA misc_feature 1755..1779 /label=Help:Barcodes CDS 1113..1754 /label=BBa_C0070 misc_feature 1095..1779 /label=BBa_I9026 misc_feature 1788..1807 /label=RhlR promoter 1806..1811 /label=-35 promoter 1829..1834 /label=-10 misc_feature 1840..1840 /label=start promoter 1788..1840 /label=BBa_R0071 RBS 1849..1863 /label=RBS-1\Strong RBS 1856..1860 /label=RBS misc_feature 1849..1863 /label=BBa_B0030 RBS 1849..1863 /label=BBa_B0030 CDS 1870..2589 /label=GFP protein CDS 1870..2589 /label=BBa_E0040 misc_feature 1788..2589 /label=BBa_K575012 promoter 2598..2632 /label=BBa_J23119 RBS 2641..2655 /label=RBS-1\Strong RBS 2648..2652 /label=RBS misc_feature 2641..2655 /label=BBa_B0030 RBS 2641..2655 /label=BBa_B0030 CDS 2662..3390 /label=BBa_C0171 misc_feature 2598..3390 /label=BBa_K575006 misc_feature 1788..3390 /label=BBa_K575032 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag 121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt 181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg 241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac 301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga 361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg 421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc 481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca 541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg 601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat 661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg 721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta 781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg 961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg 1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac 1081 gaaagctact agagaaagag gagaaatact agatgatcga actgctgtcc gaatccctgg 1141 aaggtctgtc cgctgctatg atcgctgaac tgggtcgtta ccgtcaccag gttttcatcg 1201 aaaaactggg ttgggacgtt gtttccacct cccgtgttcg tgaccaggag ttcgaccagt 1261 tcgaccaccc gcagacccgt tacatcgttg ctatgtcccg tcagggtatc tgcggttgcg 1321 ctcgtctgct gccgaccacc gacgcttacc tgctgaaaga cgttttcgct tacctgtgct 1381 ccgaaacccc gccgtccgac ccgtccgttt gggaactgtc ccgttacgct gcttccgctg 1441 ctgacgaccc gcagctggct atgaaaatct tctggtcctc cctccagtgc gcttggtacc 1501 tgggtgcttc ctccgttgtt gctgttacca ccaccgctat ggaacgttac ttcgttcgta 1561 acggtgttat cctccagcgt ctgggtccgc cgcagaaagt taaaggtgaa accctggttg 1621 ctatctcctt cccggcttac caggaacgtg gtctggaaat gctgctgcgt taccacccgg 1681 aatggctcca gggtgttccg ctgtccatgg ctgttgctgc aaacgacgaa aactacgctt 1741 tagtagctta ataactctga tagtgctagt gtagatctct actagagtcc tgtgaaatct 1801 ggcagttacc gttagctttc gaattggcta aaaagtgttc tactagagat taaagaggag 1861 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 1921 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 1981 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 2041 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 2101 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 2161 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 2221 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 2281 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 2341 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 2401 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 2461 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 2521 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 2581 aaataataat actagagttg acagctagct cagtcctagg tataatgcta gctactagag 2641 attaaagagg agaaatacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt 2701 ttgcgtagcg agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag 2761 gaagtgcggc gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc 2821 acccggccga agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag 2881 atgcagaact acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg 2941 gtggtctgga gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat 3001 tggggcctct gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg 3061 ctttccgtgg cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg 3121 cggctgcgtt gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg 3181 ctgatgtcca acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc 3241 gacggcaaga gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac 3301 ttccaccaca agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc 3361 tacgccgcgg cgctgggtct catctaataa //