##gff-version 3
##sequence-region BBa_K1651000 1 3390
BBa_K1651000	.	engineered_region	1	921	.	+	0	ID=BBa_K553003;Name=BBa_K553003
BBa_K1651000	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K553003
BBa_K1651000	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1651000	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K553003
BBa_K1651000	.	terminator	793	921	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K553003
BBa_K1651000	.	terminator	881	921	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1651000	.	engineered_region	881	921	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1651000	.	stem_loop	888	907	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1651000	.	polyA_site	908	921	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1651000	.	stop_codon	914	914	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1651000	.	terminator	793	872	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1651000	.	engineered_region	793	872	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1651000	.	stem_loop	804	847	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1651000	.	CDS	62	784	.	+	0	ID=BBa_C0179;Name=BBa_C0179;Parent=BBa_K553003
BBa_K1651000	.	engineered_region	1788	3390	.	+	0	ID=BBa_K575032;Name=BBa_K575032
BBa_K1651000	.	engineered_region	2598	3390	.	+	0	ID=BBa_K575006;Name=BBa_K575006;Parent=BBa_K575032
BBa_K1651000	.	CDS	2662	3390	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_K575006
BBa_K1651000	.	promoter	2598	2632	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K575006
BBa_K1651000	.	ribosome_entry_site	2641	2655	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575006
BBa_K1651000	.	ribosome_entry_site	2648	2652	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1651000	.	engineered_region	2641	2655	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1651000	.	ribosome_entry_site	2641	2655	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1651000	.	engineered_region	1788	2589	.	+	0	ID=BBa_K575012;Name=BBa_K575012;Parent=BBa_K575032
BBa_K1651000	.	CDS	1870	2589	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K575012
BBa_K1651000	.	CDS	1870	2589	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1651000	.	ribosome_entry_site	1849	1863	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575012
BBa_K1651000	.	ribosome_entry_site	1856	1860	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1651000	.	engineered_region	1849	1863	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1651000	.	ribosome_entry_site	1849	1863	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1651000	.	promoter	1788	1840	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K575012
BBa_K1651000	.	promoter	1829	1834	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1651000	.	promoter	1806	1811	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1651000	.	non_covalent_binding_site	1788	1807	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1651000	.	start_codon	1840	1840	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1651000	.	promoter	930	1086	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K1651000	.	non_covalent_binding_site	975	992	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K1651000	.	non_covalent_binding_site	1035	1054	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K1651000	.	promoter	1069	1074	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K1651000	.	promoter	1046	1051	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K1651000	.	engineered_region	1095	1779	.	+	0	ID=BBa_I9026;Name=BBa_I9026
BBa_K1651000	.	CDS	1113	1754	.	+	0	ID=BBa_C0070;Name=BBa_C0070;Parent=BBa_I9026
BBa_K1651000	.	CDS	1113	1715	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_K1651000	.	tag	1716	1748	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_K1651000	.	engineered_tag	1755	1779	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_K1651000	.	ribosome_entry_site	1095	1106	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I9026
BBa_K1651000	.	conserved_region	1099	1102	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1651000
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggt
tttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattct
cgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgc
ctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccg
attttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgt
atgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggc
cgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccgga
ctggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg
gcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcg
gaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtc
ccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaat
caaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaa
tactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtc
accaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcga
ccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgac
gcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtccc
gttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacct
gggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgt
ctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaa
tgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgc
tttagtagcttaataactctgatagtgctagtgtagatctctactagagtcctgtgaaatctggcagttaccgttagct
ttcgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagttgacagcta
gctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgaggaatgacggaggctttttgc
tgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagt
gcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccat
ggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcc
tgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattg
gggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccag
cagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctga
ccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgc
cgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatc
cagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa