LOCUS       BBa_K1651001            1779 bp    DNA     linear       30-Dec-2025
DEFINITION  J23100+LasR+LasR/PAI1 Inducible Promoter+RhlI
ACCESSION   BBa_K1651001
VERSION     BBa_K1651001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=BBa_C0179
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     terminator      793..921
                     /label=BBa_B0015
     misc_feature    1..921
                     /label=BBa_K553003
     misc_feature    975..992
                     /label=OP2
     misc_feature    1035..1054
                     /label=OP1
     promoter        1046..1051
                     /label=-35
     promoter        1069..1074
                     /label=-10
     promoter        930..1086
                     /label=BBa_R0079
     misc_feature    1099..1102
                     /label=conserved
     RBS             1095..1106
                     /label=BBa_B0034
     CDS             1113..1715
                     /label=rhlL
     misc_feature    1716..1748
                     /label=LVA
     misc_feature    1755..1779
                     /label=Help:Barcodes
     CDS             1113..1754
                     /label=BBa_C0070
     misc_feature    1095..1779
                     /label=BBa_I9026
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta
      781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg
      961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg
     1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac
     1081 gaaagctact agagaaagag gagaaatact agatgatcga actgctgtcc gaatccctgg
     1141 aaggtctgtc cgctgctatg atcgctgaac tgggtcgtta ccgtcaccag gttttcatcg
     1201 aaaaactggg ttgggacgtt gtttccacct cccgtgttcg tgaccaggag ttcgaccagt
     1261 tcgaccaccc gcagacccgt tacatcgttg ctatgtcccg tcagggtatc tgcggttgcg
     1321 ctcgtctgct gccgaccacc gacgcttacc tgctgaaaga cgttttcgct tacctgtgct
     1381 ccgaaacccc gccgtccgac ccgtccgttt gggaactgtc ccgttacgct gcttccgctg
     1441 ctgacgaccc gcagctggct atgaaaatct tctggtcctc cctccagtgc gcttggtacc
     1501 tgggtgcttc ctccgttgtt gctgttacca ccaccgctat ggaacgttac ttcgttcgta
     1561 acggtgttat cctccagcgt ctgggtccgc cgcagaaagt taaaggtgaa accctggttg
     1621 ctatctcctt cccggcttac caggaacgtg gtctggaaat gctgctgcgt taccacccgg
     1681 aatggctcca gggtgttccg ctgtccatgg ctgttgctgc aaacgacgaa aactacgctt
     1741 tagtagctta ataactctga tagtgctagt gtagatctc
//