BBa_K1651001
type: ComponentDefinition
persistent identity: BBa K1651001
display id: BBa_K1651001
version: 1
was generated by: iGEM to SBOL conversion
description: J23100+LasR+LasR/PAI1 Inducible Promoter+RhlI
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T09:02:55Z
mutable provenance: Series group effect element from P.aeruginosa.
owned by: james
owned by: myers
mutable description: The PAI1 will combine with a protein named lasR which is regulated by a constitutive promoter to form a complex. The PAI1-lasR complex will then activate the pLAS to produce a protein named rhlI. The rhlI will catalyze the production of PAI2.
discontinued: false
dominant: false
experience: experience/None
group u list: _2068_
m user id: 28085
owner id: 28085
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This is a part of our previous amplifier part.
star: false
creator: YANG YUANZHAN
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K553003
component: BBa_I9026
component: BBa_R0079
sequence annotation: BBa_K553003
sequence annotation: BBa_R0079
sequence annotation: BBa_I9026
sequence: https://synbiohub.org/public/igem/BBa_K1651001_sequence/1

BBa_K553003
type: Component
persistent identity: component2458289
display id: component2458289
version: 1
top level: BBa_K1651001
definition: BBa_K553003
access: public

BBa_I9026
type: Component
persistent identity: component2458301
display id: component2458301
version: 1
top level: BBa_K1651001
definition: BBa_I9026
access: public

BBa_R0079
type: Component
persistent identity: component2458294
display id: component2458294
version: 1
top level: BBa_K1651001
definition: LasR+PAI
access: public

BBa_K553003
type: SequenceAnnotation
persistent identity: annotation2458289
display id: annotation2458289
version: 1
top level: BBa_K1651001
location: https://synbiohub.org/public/igem/BBa_K1651001/annotation2458289/range2458289/1
component: BBa_K553003

https://synbiohub.org/public/igem/BBa_K1651001/annotation2458289/range2458289/1
type: Range
persistent identity: range2458289
display id: range2458289
version: 1
top level: BBa_K1651001
direction: direction/reverse
start: 1
end: 921
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2458294
display id: annotation2458294
version: 1
top level: BBa_K1651001
location: https://synbiohub.org/public/igem/BBa_K1651001/annotation2458294/range2458294/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K1651001/annotation2458294/range2458294/1
type: Range
persistent identity: range2458294
display id: range2458294
version: 1
top level: BBa_K1651001
direction: direction/reverse
start: 930
end: 1086
orientation: inline

BBa_I9026
type: SequenceAnnotation
persistent identity: annotation2458301
display id: annotation2458301
version: 1
top level: BBa_K1651001
location: https://synbiohub.org/public/igem/BBa_K1651001/annotation2458301/range2458301/1
component: BBa_I9026

https://synbiohub.org/public/igem/BBa_K1651001/annotation2458301/range2458301/1
type: Range
persistent identity: range2458301
display id: range2458301
version: 1
top level: BBa_K1651001
direction: direction/reverse
start: 1095
end: 1779
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (LasR & PAI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Binding region for LasR protein (positive regulation)
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
top level: LasR+PAI
direction: direction/forward
start: 46
end: 63
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
top level: LasR+PAI
direction: direction/forward
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
top level: LasR+PAI
direction: direction/forward
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
top level: LasR+PAI
direction: direction/forward
start: 140
end: 145
orientation: inline

BBa_I9026
type: ComponentDefinition
persistent identity: BBa I9026
display id: BBa_I9026
version: 1
was generated by: iGEM to SBOL conversion
description: rhlI translational unit
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: false
creator: crackdots
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_B0034
component: BBa_C0070
sequence annotation: BBa_B0034
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_I9026_sequence/1

BBa_B0034
type: Component
persistent identity: component939646
display id: component939646
version: 1
top level: BBa_I9026
definition: BBa_B0034
access: public

BBa_C0070
type: Component
persistent identity: component939656
display id: component939656
version: 1
top level: BBa_I9026
definition: rhII
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939646
display id: annotation939646
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
type: Range
persistent identity: range939646
display id: range939646
version: 1
top level: BBa_I9026
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation939656
display id: annotation939656
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
type: Range
persistent identity: range939656
display id: range939656
version: 1
top level: BBa_I9026
direction: direction/forward
start: 19
end: 660
orientation: inline

BBa_K553003
type: ComponentDefinition
persistent identity: BBa K553003
display id: BBa_K553003
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive production of LasR trans-activator
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:40Z
mutable provenance: rf243f2
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 4rfr4f
discontinued: false
dominant: false
experience: experience/Works
group u list: _721_
m user id: 0
owner id: 8730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: rf23f
star: false
creator: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_B0015
component: BBa_C0179
component: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K553003_sequence/1

BBa_J23100
type: Component
persistent identity: component2130119
display id: component2130119
version: 1
top level: BBa_K553003
definition: BBa_J23100
access: public

BBa_B0015
type: Component
persistent identity: component2130129
display id: component2130129
version: 1
top level: BBa_K553003
definition: BBa_B0015
access: public

BBa_C0179
type: Component
persistent identity: component2130122
display id: component2130122
version: 1
top level: BBa_K553003
definition: lasR
access: public

BBa_B0034
type: Component
persistent identity: component2130121
display id: component2130121
version: 1
top level: BBa_K553003
definition: BBa_B0034
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2130119
display id: annotation2130119
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130119/range2130119/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K553003/annotation2130119/range2130119/1
type: Range
persistent identity: range2130119
display id: range2130119
version: 1
top level: BBa_K553003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2130121
display id: annotation2130121
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130121/range2130121/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K553003/annotation2130121/range2130121/1
type: Range
persistent identity: range2130121
display id: range2130121
version: 1
top level: BBa_K553003
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2130122
display id: annotation2130122
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130122/range2130122/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K553003/annotation2130122/range2130122/1
type: Range
persistent identity: range2130122
display id: range2130122
version: 1
top level: BBa_K553003
direction: direction/reverse
start: 62
end: 784
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2130129
display id: annotation2130129
version: 1
top level: BBa_K553003
location: https://synbiohub.org/public/igem/BBa_K553003/annotation2130129/range2130129/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K553003/annotation2130129/range2130129/1
type: Range
persistent identity: range2130129
display id: range2130129
version: 1
top level: BBa_K553003
direction: direction/reverse
start: 793
end: 921
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

rhII
type: ComponentDefinition
persistent identity: BBa C0070
display id: BBa_C0070
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3476
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Editing Check (1/28/04) (0) BB sites cleaned (1) ATG (2) TAATAA (3) Blast checks out (i.e., it's RhlI) (4) Codon changes OK (AA and tRNA usage) (5) Chassis might have conflict (w/ native autoinducer system?) (6) ssrA tag added (7) barcode added (8) Codon optimized for expression in enteric bacteria 2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlL
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0070_sequence/1

rhlL
type: SequenceAnnotation
persistent identity: annotation301203
display id: annotation301203
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
type: Range
persistent identity: range301203
display id: range301203
version: 1
top level: rhII
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306820
display id: annotation306820
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
type: Range
persistent identity: range306820
display id: range306820
version: 1
top level: rhII
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213996
display id: annotation2213996
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
type: Range
persistent identity: range2213996
display id: range2213996
version: 1
top level: rhII
direction: direction/forward
start: 643
end: 667
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0079 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1651001_sequence/1
type: Sequence
persistent identity: BBa K1651001 sequence
display id: BBa_K1651001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0079_sequence/1
type: Sequence
persistent identity: BBa R0079 sequence
display id: BBa_R0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9026_sequence/1
type: Sequence
persistent identity: BBa I9026 sequence
display id: BBa_I9026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K553003_sequence/1
type: Sequence
persistent identity: BBa K553003 sequence
display id: BBa_K553003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0070_sequence/1
type: Sequence
persistent identity: BBa C0070 sequence
display id: BBa_C0070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0179_sequence/1
type: Sequence
persistent identity: BBa C0179 sequence
display id: BBa_C0179_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z