LOCUS BBa_K1651002 1953 bp DNA linear 25-Nov-2024 DEFINITION LasI driven by pBAD ACCESSION BBa_K1651002 VERSION BBa_K1651002.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1233 /label=RBS-1\Strong RBS 1226..1230 /label=RBS misc_feature 1219..1233 /label=BBa_B0030 RBS 1219..1233 /label=BBa_B0030 CDS 1240..1842 /label=LasI misc_feature 1843..1875 /label=LVA misc_feature 1882..1906 /label=Help:Barcodes CDS 1240..1906 /label=BBa_C0078 misc_feature 1915..1953 /label=B1006 stem_loop 1924..1945 /label=modified thr terminator polyA_site 1946..1953 /label=PolyA polyA_site 1915..1923 /label=PolyA terminator 1915..1953 /label=BBa_B1006 misc_feature 1219..1953 /label=BBa_K081016 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagat taaagaggag aaatactaga tgatcgttca gatcggtcgt 1261 cgtgaagagt tcgacaaaaa actgctgggt gaaatgcaca aactgcgtgc tcaggttttc 1321 aaagaacgta aaggttggga cgtttccgtt atcgacgaaa tggaaatcga cggttacgac 1381 gctctgtccc cgtactacat gctgatccag gaagacaccc cggaagctca ggttttcggt 1441 tgctggcgta tcttcgacac caccggtccg tacatgctga aaaacacctt cccggaactg 1501 ctgcacggta aagaagctcc gtgctccccg cacatctggg aactgtcccg tttcgctatc 1561 aactccggtc agaaaggttc cctgggtttc tccgactgca ccctggaagc tatgcgtgct 1621 ctggctcgtt actccttgca gaacgacatc cagaccctgg ttaccgttac caccgttggt 1681 gttgaaaaaa tgatgatccg tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa 1741 atcggtatcg aacgtgctgt tgctctgcgt atcgaactga acgctaaaac ccagatcgct 1801 ctgtacggtg gtgttctggt tgaacagcgt ctggctgttt ccgctgctaa cgacgaaaac 1861 tacgctctgg ttgcttaata actctgatag tgctagtgta gatctctact agagaaaaaa 1921 aaaccccgcc cctgacaggg cggggttttt ttt //