LOCUS       BBa_K1653016            1408 bp    DNA     linear       27-Nov-2024
DEFINITION  ispDF.dev
ACCESSION   BBa_K1653016
VERSION     BBa_K1653016.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1271
                     /label=ispDF
     CDS             82..1271
                     /label=BBa_K1653001
     stem_loop       1291..1334
                     /label=stem_loop
     misc_feature    1280..1359
                     /label=BBa_B0010
     terminator      1280..1359
                     /label=BBa_B0010
     stem_loop       1375..1394
                     /label=T7 TE
     misc_feature    1401..1401
                     /label=stop
     polyA_site      1395..1408
                     /label=polya
     misc_feature    1368..1408
                     /label=BBa_B0012
     terminator      1368..1408
                     /label=BBa_B0012
     terminator      1280..1408
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggcaacc actcatttgg atgtttgcgc cgtggttccg
      121 gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct ctcaatcggt
      181 aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg ggtgaaacgt
      241 gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct ggcgaatcat
      301 ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct ggcaggtctg
      361 aaagccgctg gcgacgcgca gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat
      421 caggatgacc tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg ggggatcctc
      481 gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat
      541 accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc tcgtgagctg
      601 ttacatgact gtctgacgcg cgctctaaat gaaggcgcga ctattaccga cgaagcctcg
      661 gcgctggaat attgcggatt ccatcctcag ttggtcgaag gccgtgcgga taacattaaa
      721 gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac catccatcag
      781 gagaatacat aatgcgaatt ggacacggtt ttgacgtaca tgcctttggc ggtgaaggcc
      841 caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg cattctgatg
      901 gcgacgtggc gctccatgcg ttgaccgatg cattgcttgg cgcggcggcg ctgggggata
      961 tcggcaagct gttcccggat accgatccgg catttaaagg tgccgatagc cgcgagctgc
     1021 tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac gtcgatgtca
     1081 ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc gtgtttattg
     1141 ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact acggaaaaac
     1201 tgggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta ctcattaagg
     1261 caacaaaatg atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1381 ccttcgggtg ggcctttctg cgtttata
//