LOCUS BBa_K1653021 1922 bp DNA linear 30-Nov-2024 DEFINITION ObGES.dev ACCESSION BBa_K1653021 VERSION BBa_K1653021.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..1791 /label=ObGES CDS 82..1785 /label=BBa_K1653007 stem_loop 1805..1848 /label=stem_loop misc_feature 1794..1873 /label=BBa_B0010 terminator 1794..1873 /label=BBa_B0010 stem_loop 1889..1908 /label=T7 TE misc_feature 1915..1915 /label=stop polyA_site 1909..1922 /label=polya misc_feature 1882..1922 /label=BBa_B0012 terminator 1882..1922 /label=BBa_B0012 terminator 1794..1922 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgtcttgt gctcgtatta ccgttactct gccttatcgt 121 tctgctaaaa ctagcattca acgcggtatc acccactatc cggcgctgat tcgtccacgt 181 tttagcgcgt gcactccgct ggcgagcgct atgccgctgt cctccacgcc gctgatcaac 241 ggcgacaaca gccagcgtaa aaacacccgt cagcacatgg aagagtcttc ttctaagcgt 301 cgcgaatacc tgctggaaga gactacccgt aaactgcaac gtaacgacac tgaatctgta 361 gagaagctga aactgatcga caacatccag cagctgggca tcggttacta tttcgaggat 421 gcgattaacg ctgtcctgcg ttctccgttc tctactggtg aagaggacct gtttactgcg 481 gccctgcgtt tccgtctgct gcgtcacaac ggcatcgaaa tttccccgga aatcttcctg 541 aagttcaagg acgaacgcgg caaattcgac gaatccgata ctctgggtct gctgagcctg 601 tacgaagcca gcaacctggg tgttgcgggc gaggaaatcc tggaagaagc aatggagttc 661 gcggaagcgc gcctgcgtcg ctccctgagc gaacctgctg cacctctgca cggcgaagtt 721 gcacaagcac tggacgttcc gcgtcacctg cgcatggctc gtctggaagc acgtcgtttc 781 atcgaacaat atggtaaaca atccgaccac gatggcgacc tgctggagct ggcaatcctg 841 gattataatc aagtccaggc gcagcaccag tctgagctga ccgagatcat ccgttggtgg 901 aaagaactgg gcctggttga taaactgtct tttggtcgtg atcgtccgct ggaatgtttc 961 ctgtggactg taggtctgct gccagagcct aaatactctt ccgtccgtat cgaactggct 1021 aaggctattt ctatcctgct ggtcattgat gatatcttcg acacctacgg cgagatggat 1081 gatctgatcc tgttcaccga cgctatccgt cgttgggacc tggaagcgat ggaaggtctg 1141 ccggaataca tgaagatttg ctacatggcc ctgtataaca ctaccaacga ggtttgttac 1201 aaagtactgc gtgacacggg ccgtatcgtt ctgctgaatc tgaagtccac ctggattgat 1261 atgatcgagg gtttcatgga ggaagcgaag tggttcaacg gtggcagcgc cccgaaactg 1321 gaagaataca tcgaaaacgg tgtgtccacc gctggtgctt acatggcctt tgctcatatc 1381 ttcttcctga ttggtgaagg tgtaacgcac caaaactctc aactgtttac ccagaaacct 1441 tatccgaaag tgtttagcgc agccggccgt attctgcgtc tgtgggacga tctgggcacc 1501 gcgaaggaag aacaagaacg cggtgacctg gcaagctgcg ttcagctgtt catgaaagag 1561 aaatccctga ccgaggaaga agcacgttcc cgcattctgg aggagattaa aggcctgtgg 1621 cgtgacctga acggtgaact ggtttataac aaaaatctgc cgctgtccat tatcaaggtg 1681 gccctgaaca tggcacgcgc atcccaggta gtttataaac atgaccagga tacctatttc 1741 tcctccgtag ataactacgt tgacgccctg ttcttcactc aatgatacta gagccaggca 1801 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1861 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1921 ta //