LOCUS       BBa_K1653023            3048 bp    DNA     linear       14-May-2024
DEFINITION  m-ispA+ObGES.dev
ACCESSION   BBa_K1653023
VERSION     BBa_K1653023.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..981
                     /label=m-ispA
     CDS             82..981
                     /label=BBa_K1653004
     stem_loop       1001..1044
                     /label=stem_loop
     misc_feature    990..1069
                     /label=BBa_B0010
     terminator      990..1069
                     /label=BBa_B0010
     stem_loop       1085..1104
                     /label=T7 TE
     misc_feature    1111..1111
                     /label=stop
     polyA_site      1105..1118
                     /label=polya
     misc_feature    1078..1118
                     /label=BBa_B0012
     terminator      1078..1118
                     /label=BBa_B0012
     terminator      990..1118
                     /label=BBa_B0015
     misc_feature    1129..1145
                     /label=lac O1
     promoter        1146..1151
                     /label=-35
     misc_feature    1152..1168
                     /label=lac O1
     promoter        1169..1174
                     /label=-10
     misc_feature    1127..1180
                     /label=BBa_R0011
     promoter        1127..1180
                     /label=BBa_R0011
     misc_feature    1194..1197
                     /label=conserved
     RBS             1190..1201
                     /label=BBa_B0034
     CDS             1208..2917
                     /label=ObGES
     CDS             1208..2911
                     /label=BBa_K1653007
     stem_loop       2931..2974
                     /label=stem_loop
     misc_feature    2920..2999
                     /label=BBa_B0010
     terminator      2920..2999
                     /label=BBa_B0010
     stem_loop       3015..3034
                     /label=T7 TE
     misc_feature    3041..3041
                     /label=stop
     polyA_site      3035..3048
                     /label=polya
     misc_feature    3008..3048
                     /label=BBa_B0012
     terminator      3008..3048
                     /label=BBa_B0012
     terminator      2920..3048
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggacttt ccgcagcaac tcgaagcctg cgttaagcag
      121 gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc
      181 gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat
      241 gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc tgccgccgtt
      301 gagtgtatcc acgcttactt tttaattcat gatgatttac cggcaatgga tgatgacgat
      361 ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct
      421 ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgccgatat gccggaagtg
      481 tcggaccgcg acagaatttc gatgatttct gaactggcga gcgccagtgg tattgccgga
      541 atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg
      601 cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt
      661 gcattaagcg ccggagataa aggacgtcgt gctctgccgg tactcgacaa gtatgcagag
      721 agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg agatactgca
      781 acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt
      841 ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg
      901 ctgaaacaac tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac
      961 atcatccagc gtaataaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1081 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt gtgagcggat
     1141 aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga aagaggagaa
     1201 atactagatg tcttgtgctc gtattaccgt tactctgcct tatcgttctg ctaaaactag
     1261 cattcaacgc ggtatcaccc actatccggc gctgattcgt ccacgtttta gcgcgtgcac
     1321 tccgctggcg agcgctatgc cgctgtcctc cacgccgctg atcaacggcg acaacagcca
     1381 gcgtaaaaac acccgtcagc acatggaaga gtcttcttct aagcgtcgcg aatacctgct
     1441 ggaagagact acccgtaaac tgcaacgtaa cgacactgaa tctgtagaga agctgaaact
     1501 gatcgacaac atccagcagc tgggcatcgg ttactatttc gaggatgcga ttaacgctgt
     1561 cctgcgttct ccgttctcta ctggtgaaga ggacctgttt actgcggccc tgcgtttccg
     1621 tctgctgcgt cacaacggca tcgaaatttc cccggaaatc ttcctgaagt tcaaggacga
     1681 acgcggcaaa ttcgacgaat ccgatactct gggtctgctg agcctgtacg aagccagcaa
     1741 cctgggtgtt gcgggcgagg aaatcctgga agaagcaatg gagttcgcgg aagcgcgcct
     1801 gcgtcgctcc ctgagcgaac ctgctgcacc tctgcacggc gaagttgcac aagcactgga
     1861 cgttccgcgt cacctgcgca tggctcgtct ggaagcacgt cgtttcatcg aacaatatgg
     1921 taaacaatcc gaccacgatg gcgacctgct ggagctggca atcctggatt ataatcaagt
     1981 ccaggcgcag caccagtctg agctgaccga gatcatccgt tggtggaaag aactgggcct
     2041 ggttgataaa ctgtcttttg gtcgtgatcg tccgctggaa tgtttcctgt ggactgtagg
     2101 tctgctgcca gagcctaaat actcttccgt ccgtatcgaa ctggctaagg ctatttctat
     2161 cctgctggtc attgatgata tcttcgacac ctacggcgag atggatgatc tgatcctgtt
     2221 caccgacgct atccgtcgtt gggacctgga agcgatggaa ggtctgccgg aatacatgaa
     2281 gatttgctac atggccctgt ataacactac caacgaggtt tgttacaaag tactgcgtga
     2341 cacgggccgt atcgttctgc tgaatctgaa gtccacctgg attgatatga tcgagggttt
     2401 catggaggaa gcgaagtggt tcaacggtgg cagcgccccg aaactggaag aatacatcga
     2461 aaacggtgtg tccaccgctg gtgcttacat ggcctttgct catatcttct tcctgattgg
     2521 tgaaggtgta acgcaccaaa actctcaact gtttacccag aaaccttatc cgaaagtgtt
     2581 tagcgcagcc ggccgtattc tgcgtctgtg ggacgatctg ggcaccgcga aggaagaaca
     2641 agaacgcggt gacctggcaa gctgcgttca gctgttcatg aaagagaaat ccctgaccga
     2701 ggaagaagca cgttcccgca ttctggagga gattaaaggc ctgtggcgtg acctgaacgg
     2761 tgaactggtt tataacaaaa atctgccgct gtccattatc aaggtggccc tgaacatggc
     2821 acgcgcatcc caggtagttt ataaacatga ccaggatacc tatttctcct ccgtagataa
     2881 ctacgttgac gccctgttct tcactcaatg atactagagc caggcatcaa ataaaacgaa
     2941 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     3001 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//