BBa_K1653023
type: ComponentDefinition
persistent identity: BBa K1653023
display id: BBa_K1653023
version: 1
was generated by: iGEM to SBOL conversion
description: m-ispA+ObGES.dev
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T07:06:58Z
mutable provenance: soon
owned by: james
owned by: myers
mutable description: soon
discontinued: false
dominant: false
group u list: _2071_
m user id: 22434
owner id: 22434
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: soon
star: false
creator: Daiki Haraguchi
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0011
component: BBa_B0015
component: BBa_K1653004
component: BBa_B0034
component: BBa_K1653007
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K1653004
sequence annotation: BBa_B0015
sequence annotation: BBa_K1653007
sequence: https://synbiohub.org/public/igem/BBa_K1653023_sequence/1

BBa_R0011
type: Component
persistent identity: component2441866
display id: component2441866
version: 1
top level: BBa_K1653023
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2441865
display id: component2441865
version: 1
top level: BBa_K1653023
definition: BBa_B0015
access: public

BBa_K1653004
type: Component
persistent identity: component2441858
display id: component2441858
version: 1
top level: BBa_K1653023
definition: BBa_K1653004
access: public

BBa_B0034
type: Component
persistent identity: component2441856
display id: component2441856
version: 1
top level: BBa_K1653023
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2441880
display id: component2441880
version: 1
top level: BBa_K1653023
definition: BBa_B0015
access: public

BBa_K1653007
type: Component
persistent identity: component2441873
display id: component2441873
version: 1
top level: BBa_K1653023
definition: BBa_K1653007
access: public

BBa_B0034
type: Component
persistent identity: component2441872
display id: component2441872
version: 1
top level: BBa_K1653023
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2441850
display id: component2441850
version: 1
top level: BBa_K1653023
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2441850
display id: annotation2441850
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441850/range2441850/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441850/range2441850/1
type: Range
persistent identity: range2441850
display id: range2441850
version: 1
top level: BBa_K1653023
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2441856
display id: annotation2441856
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441856/range2441856/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441856/range2441856/1
type: Range
persistent identity: range2441856
display id: range2441856
version: 1
top level: BBa_K1653023
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_K1653004
type: SequenceAnnotation
persistent identity: annotation2441858
display id: annotation2441858
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441858/range2441858/1
component: BBa_K1653004

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441858/range2441858/1
type: Range
persistent identity: range2441858
display id: range2441858
version: 1
top level: BBa_K1653023
direction: direction/reverse
start: 82
end: 981
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2441865
display id: annotation2441865
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441865/range2441865/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441865/range2441865/1
type: Range
persistent identity: range2441865
display id: range2441865
version: 1
top level: BBa_K1653023
direction: direction/reverse
start: 990
end: 1118
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2441866
display id: annotation2441866
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441866/range2441866/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441866/range2441866/1
type: Range
persistent identity: range2441866
display id: range2441866
version: 1
top level: BBa_K1653023
direction: direction/forward
start: 1127
end: 1180
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2441872
display id: annotation2441872
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441872/range2441872/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441872/range2441872/1
type: Range
persistent identity: range2441872
display id: range2441872
version: 1
top level: BBa_K1653023
direction: direction/reverse
start: 1190
end: 1201
orientation: inline

BBa_K1653007
type: SequenceAnnotation
persistent identity: annotation2441873
display id: annotation2441873
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441873/range2441873/1
component: BBa_K1653007

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441873/range2441873/1
type: Range
persistent identity: range2441873
display id: range2441873
version: 1
top level: BBa_K1653023
direction: direction/reverse
start: 1208
end: 2911
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2441880
display id: annotation2441880
version: 1
top level: BBa_K1653023
location: https://synbiohub.org/public/igem/BBa_K1653023/annotation2441880/range2441880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1653023/annotation2441880/range2441880/1
type: Range
persistent identity: range2441880
display id: range2441880
version: 1
top level: BBa_K1653023
direction: direction/reverse
start: 2920
end: 3048
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K1653004
type: ComponentDefinition
persistent identity: BBa K1653004
display id: BBa_K1653004
version: 1
was generated by: iGEM to SBOL conversion
description: m-ispA
created: 2015-05-30T11:00:00Z
modified: 2015-09-18T03:59:15Z
mutable provenance: next time
owned by: james
owned by: myers
mutable description: next time
discontinued: false
dominant: false
experience: experience/None
group u list: _2071_
m user id: 22434
owner id: 22434
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: next time
star: false
creator: Daiki Haraguchi, Kyohei Takekawa
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: m-ispA
sequence: https://synbiohub.org/public/igem/BBa_K1653004_sequence/1

m-ispA
type: SequenceAnnotation
persistent identity: annotation2445843
display id: annotation2445843
version: 1
top level: BBa_K1653004
location: https://synbiohub.org/public/igem/BBa_K1653004/annotation2445843/range2445843/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1653004/annotation2445843/range2445843/1
type: Range
persistent identity: range2445843
display id: range2445843
version: 1
top level: BBa_K1653004
direction: direction/forward
start: 1
end: 900
orientation: inline

BBa_K1653007
type: ComponentDefinition
persistent identity: BBa K1653007
display id: BBa_K1653007
version: 1
was generated by: iGEM to SBOL conversion
description: ObGES
created: 2015-05-30T11:00:00Z
modified: 2015-09-08T02:23:14Z
mutable provenance: next time
owned by: james
owned by: myers
mutable description: next time
discontinued: false
dominant: false
experience: experience/None
group u list: _2071_
m user id: 24944
owner id: 22434
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: next time
star: false
creator: Daiki Haraguchi
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ObGES
sequence: https://synbiohub.org/public/igem/BBa_K1653007_sequence/1

ObGES
type: SequenceAnnotation
persistent identity: annotation2446618
display id: annotation2446618
version: 1
top level: BBa_K1653007
location: https://synbiohub.org/public/igem/BBa_K1653007/annotation2446618/range2446618/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1653007/annotation2446618/range2446618/1
type: Range
persistent identity: range2446618
display id: range2446618
version: 1
top level: BBa_K1653007
direction: direction/forward
start: 1
end: 1710
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1653023_sequence/1
type: Sequence
persistent identity: BBa K1653023 sequence
display id: BBa_K1653023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggactttccgcagcaactcgaagcctgcgttaagcaggccaaccaggcgctgagccgttttatcgccccactgccctttcagaacactcccgtggtcgaaaccatgcagtatggcgcattattaggtggtaagcgcctgcgacctttcctggtttatgccaccggtcatatgttcggcgttagcacaaacacgctggacgcacccgctgccgccgttgagtgtatccacgcttactttttaattcatgatgatttaccggcaatggatgatgacgatctgcgtcgcggtttgccaacctgccatgtgaagtttggcgaagcaaacgcgattctcgctggcgacgctttacaaacgctggcgttctcgattttaagcgatgccgatatgccggaagtgtcggaccgcgacagaatttcgatgatttctgaactggcgagcgccagtggtattgccggaatgtgcggtggtcaggcattagatttagacgcggaaggcaaacacgtacctctggacgcgcttgagcgtattcatcgtcataaaaccggcgcattgattcgcgccgccgttcgccttggtgcattaagcgccggagataaaggacgtcgtgctctgccggtactcgacaagtatgcagagagcatcggccttgccttccaggttcaggatgacatcctggatgtggtgggagatactgcaacgttgggaaaacgccagggtgccgaccagcaacttggtaaaagtacctaccctgcacttctgggtcttgagcaagcccggaagaaagcccgggatctgatcgacgatgcccgtcagtcgctgaaacaactggctgaacagtcactcgatacctcggcactggaagcgctagcggactacatcatccagcgtaataaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtcttgtgctcgtattaccgttactctgccttatcgttctgctaaaactagcattcaacgcggtatcacccactatccggcgctgattcgtccacgttttagcgcgtgcactccgctggcgagcgctatgccgctgtcctccacgccgctgatcaacggcgacaacagccagcgtaaaaacacccgtcagcacatggaagagtcttcttctaagcgtcgcgaatacctgctggaagagactacccgtaaactgcaacgtaacgacactgaatctgtagagaagctgaaactgatcgacaacatccagcagctgggcatcggttactatttcgaggatgcgattaacgctgtcctgcgttctccgttctctactggtgaagaggacctgtttactgcggccctgcgtttccgtctgctgcgtcacaacggcatcgaaatttccccggaaatcttcctgaagttcaaggacgaacgcggcaaattcgacgaatccgatactctgggtctgctgagcctgtacgaagccagcaacctgggtgttgcgggcgaggaaatcctggaagaagcaatggagttcgcggaagcgcgcctgcgtcgctccctgagcgaacctgctgcacctctgcacggcgaagttgcacaagcactggacgttccgcgtcacctgcgcatggctcgtctggaagcacgtcgtttcatcgaacaatatggtaaacaatccgaccacgatggcgacctgctggagctggcaatcctggattataatcaagtccaggcgcagcaccagtctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1653004_sequence/1
type: Sequence
persistent identity: BBa K1653004 sequence
display id: BBa_K1653004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggactttccgcagcaactcgaagcctgcgttaagcaggccaaccaggcgctgagccgttttatcgccccactgccctttcagaacactcccgtggtcgaaaccatgcagtatggcgcattattaggtggtaagcgcctgcgacctttcctggtttatgccaccggtcatatgttcggcgttagcacaaacacgctggacgcacccgctgccgccgttgagtgtatccacgcttactttttaattcatgatgatttaccggcaatggatgatgacgatctgcgtcgcggtttgccaacctgccatgtgaagtttggcgaagcaaacgcgattctcgctggcgacgctttacaaacgctggcgttctcgattttaagcgatgccgatatgccggaagtgtcggaccgcgacagaatttcgatgatttctgaactggcgagcgccagtggtattgccggaatgtgcggtggtcaggcattagatttagacgcggaaggcaaacacgtacctctggacgcgcttgagcgtattcatcgtcataaaaccggcgcattgattcgcgccgccgttcgccttggtgcattaagcgccggagataaaggacgtcgtgctctgccggtactcgacaagtatgcagagagcatcggccttgccttccaggttcaggatgacatcctggatgtggtgggagatactgcaacgttgggaaaacgccagggtgccgaccagcaacttggtaaaagtacctaccctgcacttctgggtcttgagcaagcccggaagaaagcccgggatctgatcgacgatgcccgtcagtcgctgaaacaactggctgaacagtcactcgatacctcggcactggaagcgctagcggactacatcatccagcgtaataaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1653007_sequence/1
type: Sequence
persistent identity: BBa K1653007 sequence
display id: BBa_K1653007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcttgtgctcgtattaccgttactctgccttatcgttctgctaaaactagcattcaacgcggtatcacccactatccggcgctgattcgtccacgttttagcgcgtgcactccgctggcgagcgctatgccgctgtcctccacgccgctgatcaacggcgacaacagccagcgtaaaaacacccgtcagcacatggaagagtcttcttctaagcgtcgcgaatacctgctggaagagactacccgtaaactgcaacgtaacgacactgaatctgtagagaagctgaaactgatcgacaacatccagcagctgggcatcggttactatttcgaggatgcgattaacgctgtcctgcgttctccgttctctactggtgaagaggacctgtttactgcggccctgcgtttccgtctgctgcgtcacaacggcatcgaaatttccccggaaatcttcctgaagttcaaggacgaacgcggcaaattcgacgaatccgatactctgggtctgctgagcctgtacgaagccagcaacctgggtgttgcgggcgaggaaatcctggaagaagcaatggagttcgcggaagcgcgcctgcgtcgctccctgagcgaacctgctgcacctctgcacggcgaagttgcacaagcactggacgttccgcgtcacctgcgcatggctcgtctggaagcacgtcgtttcatcgaacaatatggtaaacaatccgaccacgatggcgacctgctggagctggcaatcctggattataatcaagtccaggcgcagcaccagtctgagctgaccgagatcatccgttggtggaaagaactgggcctggttgataaactgtcttttggtcgtgatcgtccgctggaatgtttcctgtggactgtaggtctgctgccagagcctaaatactcttccgtccgtatcgaactggctaaggctatttctatcctgctggtcattgatgatatcttcgacacctacggcgagatggatgatctgatcctgttcaccgacgctatccgtcgttgggacctggaagcgatggaaggtctgccggaatacatgaagatttgctacatggccctgtataacactaccaacgaggtttgttacaaagtactgcgtgacacgggccgtatcgttctgctgaatctgaagtccacctggattgatatgatcgagggtttcatggaggaagcgaagtggttcaacggtggcagcgccccgaaactggaagaatacatcgaaaacggtgtgtccaccgctggtgcttacatggcctttgctcatatcttcttcctgattggtgaaggtgtaacgcaccaaaactctcaactgtttacccagaaaccttatccgaaagtgtttagcgcagccggccgtattctgcgtctgtgggacgatctgggcaccgcgaaggaagaacaagaacgcggtgacctggcaagctgcgttcagctgttcatgaaagagaaatccctgaccgaggaagaagcacgttcccgcattctggaggagattaaaggcctgtggcgtgacctgaacggtgaactggtttataacaaaaatctgccgctgtccattatcaaggtggccctgaacatggcacgcgcatcccaggtagtttataaacatgaccaggatacctatttctcctccgtagataactacgttgacgccctgttcttcactcaatgatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z