LOCUS BBa_K1653024 3872 bp DNA linear 26-Nov-2024 DEFINITION Terpene precursor mass-production device ACCESSION BBa_K1653024 VERSION BBa_K1653024.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..1944 /label=dxs CDS 82..1944 /label=BBa_K1653000 misc_feature 1957..1960 /label=conserved RBS 1953..1964 /label=BBa_B0034 CDS 1971..2519 /label=idi CDS 1971..2519 /label=BBa_K1653002 misc_feature 2532..2535 /label=conserved RBS 2528..2539 /label=BBa_B0034 CDS 2546..3735 /label=ispDF CDS 2546..3735 /label=BBa_K1653001 stem_loop 3755..3798 /label=stem_loop misc_feature 3744..3823 /label=BBa_B0010 terminator 3744..3823 /label=BBa_B0010 stem_loop 3839..3858 /label=T7 TE misc_feature 3865..3865 /label=stop polyA_site 3859..3872 /label=polya misc_feature 3832..3872 /label=BBa_B0012 terminator 3832..3872 /label=BBa_B0012 terminator 3744..3872 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagtttt gatattgcca aatacccgac cctggcactg 121 gtcgactcca cccaggagtt acgactgttg ccgaaagaga gtttaccgaa actctgcgac 181 gaactgcgcc gctatttact cgacagcgtg agccgttcca gcgggcactt cgcctccggg 241 ctgggcacgg tcgaactgac cgtggcgctg cactatgtct acaacacccc gtttgaccaa 301 ttgatttggg atgtggggca tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac 361 aaaatcggca ccatccgtca gaaaggcggt ctgcacccgt tcccgtggcg cggcgaaagc 421 gaatatgacg tattaagcgt cgggcattca tcaacctcca tcagtgccgg aattggtatt 481 gcggttgctg ccgaaaaaga aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc 541 gcgattaccg caggcatggc gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat 601 atgctggtga ttctcaacga caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaac 661 aaccatctgg cacagctgct ttccggtaag ctttactctt cactgcgcga aggcgggaaa 721 aaagttttct ctggcgtgcc gccaattaaa gagctgctca aacgcaccga agaacatatt 781 aaaggcatgg tagtgcctgg cacgttgttt gaagagctgg gctttaacta catcggcccg 841 gtggacggtc acgatgtgct ggggcttatc accacgctaa agaacatgcg cgacctgaaa 901 ggcccgcagt tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa 961 gacccgatca ctttccacgc cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa 1021 agtagcggcg gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca 1081 gcgaaagaca acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc 1141 gagttttcac gtaaattccc ggatcgctac ttcgacgtgg caattgccga gcaacacgcg 1201 gtgacctttg ctgcgggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc 1261 actttcctgc aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagcttccg 1321 gtcctgttcg ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggt 1381 gcttttgatc tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat 1441 gaaaacgaat gtcgccagat gctctatacc ggctatcact ataacgatgg cccgtcagcg 1501 gtgcgctacc cgcgtggcaa cgcggtcggc gtggaactga cgccgctgga aaaactacca 1561 attggcaaag gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg 1621 ctgatgccag aagcggcgaa agtcgccgaa tcgctgaacg ccacgctggt cgatatgcgt 1681 tttgtgaaac cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg 1741 gtcaccgtag aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgctg 1801 atggcccatc gtaaaccagt acccgtgctg aacattggcc tgccggactt ctttattccg 1861 caaggaactc aggaagaaat gcgcgccgaa ctcggcctcg atgccgctgg tatggaagcc 1921 aaaatcaagg cctggctggc ataatactag agaaagagga gaaatactag atgcaaacgg 1981 aacacgtcat tttattgaat gcacagggag ttcccacggg tacgctggaa aagtatgccg 2041 cacacacggc agacacccgc ttacatctcg cgttctccag ttggctgttt aatgccaaag 2101 gacaattatt agttacccgc cgcgcactga gcaaaaaagc atggcctggc gtgtggacta 2161 actcggtttg tgggcaccca caactgggag aaagcaacga agacgcagtg atccgccgtt 2221 gccgttatga gcttggcgtg gaaattacgc ctcctgaatc tatctatcct gactttcgct 2281 accgcgccac cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta tttgccgcac 2341 gcaccactag cgcgttacag atcaatgatg atgaagtgat ggattatcaa tggtgtgatt 2401 tagcagatgt attacacggt attgatgcca cgccgtgggc gttcagtccg tggatggtga 2461 tgcaggcgac aaatcgcgaa gccagaaaac gattatctgc atttacccag cttaaataat 2521 actagagaaa gaggagaaat actagatggc aaccactcat ttggatgttt gcgccgtggt 2581 tccggcggcc ggatttggcc gtcgaatgca aacggaatgt cctaagcaat atctctcaat 2641 cggtaatcaa accattcttg aacactcggt gcatgcgctg ctggcgcatc cccgggtgaa 2701 acgtgtcgtc attgccataa gtcctggcga tagccgtttt gcacaacttc ctctggcgaa 2761 tcatccgcaa atcaccgttg tagatggcgg tgatgagcgt gccgattccg tgctggcagg 2821 tctgaaagcc gctggcgacg cgcagtgggt attggtgcat gacgccgctc gtccttgttt 2881 gcatcaggat gacctcgcgc gattgttggc gttgagcgaa accagccgca cgggggggat 2941 cctcgccgca ccagtgcgcg atactatgaa acgtgccgaa ccgggcaaaa atgccattgc 3001 tcataccgtt gatcgcaacg gcttatggca cgcgctgacg ccgcaatttt tccctcgtga 3061 gctgttacat gactgtctga cgcgcgctct aaatgaaggc gcgactatta ccgacgaagc 3121 ctcggcgctg gaatattgcg gattccatcc tcagttggtc gaaggccgtg cggataacat 3181 taaagtcacg cgcccggaag atttggcact ggccgagttt tacctcaccc gaaccatcca 3241 tcaggagaat acataatgcg aattggacac ggttttgacg tacatgcctt tggcggtgaa 3301 ggcccaatta tcattggtgg cgtacgcatt ccttacgaaa aaggattgct ggcgcattct 3361 gatggcgacg tggcgctcca tgcgttgacc gatgcattgc ttggcgcggc ggcgctgggg 3421 gatatcggca agctgttccc ggataccgat ccggcattta aaggtgccga tagccgcgag 3481 ctgctacgcg aagcctggcg tcgtattcag gcgaagggtt atacccttgg caacgtcgat 3541 gtcactatca tcgctcaggc accgaagatg ttgccgcaca ttccacaaat gcgcgtgttt 3601 attgccgaag atctcggctg ccatatggat gatgttaacg tgaaagccac tactacggaa 3661 aaactgggat ttaccggacg tggggaaggg attgcctgtg aagcggtggc gctactcatt 3721 aaggcaacaa aatgatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3781 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 3841 ctcaccttcg ggtgggcctt tctgcgttta ta //