##gff-version 3
##sequence-region BBa_K1653025 1 4998
BBa_K1653025	.	terminator	4870	4998	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653025	.	terminator	4870	4949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653025	.	stem_loop	4881	4924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653025	.	engineered_region	4870	4949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653025	.	terminator	4958	4998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653025	.	stop_codon	4991	4991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653025	.	polyA_site	4985	4998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653025	.	stem_loop	4965	4984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653025	.	engineered_region	4958	4998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653025	.	CDS	3097	3645	.	+	0	ID=BBa_K1653002;Name=BBa_K1653002
BBa_K1653025	.	CDS	3097	3645	.	+	0	ID=annotation2432275;Name=idi;Parent=BBa_K1653002
BBa_K1653025	.	CDS	1208	3070	.	+	0	ID=BBa_K1653000;Name=BBa_K1653000
BBa_K1653025	.	CDS	1208	3070	.	+	0	ID=annotation2432264;Name=dxs;Parent=BBa_K1653000
BBa_K1653025	.	ribosome_entry_site	3079	3090	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653025	.	conserved_region	3083	3086	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653025	.	ribosome_entry_site	3654	3665	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653025	.	conserved_region	3658	3661	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653025	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653025	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653025	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653025	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653025	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653025	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653025	.	ribosome_entry_site	1190	1201	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653025	.	conserved_region	1194	1197	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653025	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653025	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653025	.	CDS	82	981	.	+	0	ID=BBa_K1653003;Name=BBa_K1653003
BBa_K1653025	.	CDS	82	981	.	+	0	ID=annotation2432401;Name=ispA;Parent=BBa_K1653003
BBa_K1653025	.	promoter	1127	1180	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653025	.	promoter	1169	1174	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653025	.	operator	1129	1145	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653025	.	promoter	1146	1151	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653025	.	engineered_region	1127	1180	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653025	.	operator	1152	1168	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653025	.	CDS	3672	4861	.	+	0	ID=BBa_K1653001;Name=BBa_K1653001
BBa_K1653025	.	CDS	3672	4861	.	+	0	ID=annotation2445940;Name=ispDF;Parent=BBa_K1653001
BBa_K1653025	.	terminator	990	1118	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653025	.	terminator	990	1069	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653025	.	stem_loop	1001	1044	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653025	.	engineered_region	990	1069	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653025	.	terminator	1078	1118	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653025	.	stop_codon	1111	1111	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653025	.	polyA_site	1105	1118	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653025	.	stem_loop	1085	1104	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653025	.	engineered_region	1078	1118	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1653025
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggactttccgcagcaactcgaagcctgcgttaagcaggccaaccaggcgctgagccgttttatcgccccactgcc
ctttcagaacactcccgtggtcgaaaccatgcagtatggcgcattattaggtggtaagcgcctgcgacctttcctggtt
tatgccaccggtcatatgttcggcgttagcacaaacacgctggacgcacccgctgccgccgttgagtgtatccacgctt
actcattaattcatgatgatttaccggcaatggatgatgacgatctgcgtcgcggtttgccaacctgccatgtgaagtt
tggcgaagcaaacgcgattctcgctggcgacgctttacaaacgctggcgttctcgattttaagcgatgccgatatgccg
gaagtgtcggaccgcgacagaatttcgatgatttctgaactggcgagcgccagtggtattgccggaatgtgcggtggtc
aggcattagatttagacgcggaaggcaaacacgtacctctggacgcgcttgagcgtattcatcgtcataaaaccggcgc
attgattcgcgccgccgttcgccttggtgcattaagcgccggagataaaggacgtcgtgctctgccggtactcgacaag
tatgcagagagcatcggccttgccttccaggttcaggatgacatcctggatgtggtgggagatactgcaacgttgggaa
aacgccagggtgccgaccagcaacttggtaaaagtacctaccctgcacttctgggtcttgagcaagcccggaagaaagc
ccgggatctgatcgacgatgcccgtcagtcgctgaaacaactggctgaacagtcactcgatacctcggcactggaagcg
ctagcggactacatcatccagcgtaataaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatact
agagaaagaggagaaatactagatgagttttgatattgccaaatacccgaccctggcactggtcgactccacccaggag
ttacgactgttgccgaaagagagtttaccgaaactctgcgacgaactgcgccgctatttactcgacagcgtgagccgtt
ccagcgggcacttcgcctccgggctgggcacggtcgaactgaccgtggcgctgcactatgtctacaacaccccgtttga
ccaattgatttgggatgtggggcatcaggcttatccgcataaaattttgaccggacgccgcgacaaaatcggcaccatc
cgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagcgaatatgacgtattaagcgtcgggcattcatcaa
cctccatcagtgccggaattggtattgcggttgctgccgaaaaagaaggcaaaaatcgccgcaccgtctgtgtcattgg
cgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatcacgcgggcgatatccgtcctgatatgctggtgatt
ctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctcaacaaccatctggcacagctgctttccggtaagc
tttactcttcactgcgcgaaggcgggaaaaaagttttctctggcgtgccgccaattaaagagctgctcaaacgcaccga
agaacatattaaaggcatggtagtgcctggcacgttgtttgaagagctgggctttaactacatcggcccggtggacggt
cacgatgtgctggggcttatcaccacgctaaagaacatgcgcgacctgaaaggcccgcagttcctgcatatcatgacca
aaaaaggtcgtggttatgaaccggcagaaaaagacccgatcactttccacgccgtgcctaaatttgatccctccagcgg
ttgtttgccgaaaagtagcggcggtttgccgagctattcaaaaatctttggcgactggttgtgcgaaacggcagcgaaa
gacaacaagctgatggcgattactccggcgatgcgtgaaggttccggcatggtcgagttttcacgtaaattcccggatc
gctacttcgacgtggcaattgccgagcaacacgcggtgacctttgctgcgggtctggcgattggtgggtacaaacccat
tgtcgcgatttactccactttcctgcaacgcgcctatgatcaggtgctgcatgacgtggcgattcaaaagcttccggtc
ctgttcgccatcgaccgcgcgggcattgttggtgctgacggtcaaacccatcagggtgcttttgatctctcttacctgc
gctgcataccggaaatggtcattatgaccccgagcgatgaaaacgaatgtcgccagatgctctataccggctatcacta
taacgatggcccgtcagcggtgcgctacccgcgtggcaacgcggtcggcgtggaactgacgccgctggaaaaactacca
attggcaaaggcattgtgaagcgtcgtggcgagaaactggcgatccttaactttggtacgctgatgccagaagcggcga
aagtcgccgaatcgctgaacgccacgctggtcgatatgcgttttgtgaaaccgcttgatgaagcgttaattctggaaat
ggccgccagccatgaagcgctggtcaccgtagaagaaaacgccattatgggcggcgcaggcagcggcgtgaacgaagtg
ctgatggcccatcgtaaaccagtacccgtgctgaacattggcctgccggacttctttattccgcaaggaactcaggaag
aaatgcgcgccgaactcggcctcgatgccgctggtatggaagccaaaatcaaggcctggctggcataatactagagaaa
gaggagaaatactagatgcaaacggaacacgtcattttattgaatgcacagggagttcccacgggtacgctggaaaagt
atgccgcacacacggcagacacccgcttacatctcgcgttctccagttggctgtttaatgccaaaggacaattattagt
tacccgccgcgcactgagcaaaaaagcatggcctggcgtgtggactaactcggtttgtgggcacccacaactgggagaa
agcaacgaagacgcagtgatccgccgttgccgttatgagcttggcgtggaaattacgcctcctgaatctatctatcctg
actttcgctaccgcgccaccgatccgagtggcattgtggaaaatgaagtgtgtccggtatttgccgcacgcaccactag
cgcgttacagatcaatgatgatgaagtgatggattatcaatggtgtgatttagcagatgtattacacggtattgatgcc
acgccgtgggcgttcagtccgtggatggtgatgcaggcgacaaatcgcgaagccagaaaacgattatctgcatttaccc
agcttaaataatactagagaaagaggagaaatactagatggcaaccactcatttggatgtttgcgccgtggttccggcg
gccggatttggccgtcgaatgcaaacggaatgtcctaagcaatatctctcaatcggtaatcaaaccattcttgaacact
cggtgcatgcgctgctggcgcatccccgggtgaaacgtgtcgtcattgccataagtcctggcgatagccgttttgcaca
acttcctctggcgaatcatccgcaaatcaccgttgtagatggcggtgatgagcgtgccgattccgtgctggcaggtctg
aaagccgctggcgacgcgcagtgggtattggtgcatgacgccgctcgtccttgtttgcatcaggatgacctcgcgcgat
tgttggcgttgagcgaaaccagccgcacgggggggatcctcgccgcaccagtgcgcgatactatgaaacgtgccgaacc
gggcaaaaatgccattgctcataccgttgatcgcaacggcttatggcacgcgctgacgccgcaatttttccctcgtgag
ctgttacatgactgtctgacgcgcgctctaaatgaaggcgcgactattaccgacgaagcctcggcgctggaatattgcg
gattccatcctcagttggtcgaaggccgtgcggataacattaaagtcacgcgcccggaagatttggcactggccgagtt
ttacctcacccgaaccatccatcaggagaatacataatgcgaattggacacggttttgacgtacatgcctttggcggtg
aaggcccaattatcattggtggcgtacgcattccttacgaaaaaggattgctggcgcattctgatggcgacgtggcgct
ccatgcgttgaccgatgcattgcttggcgcggcggcgctgggggatatcggcaagctgttcccggataccgatccggca
tttaaaggtgccgatagccgcgagctgctacgcgaagcctggcgtcgtattcaggcgaagggttatacccttggcaacg
tcgatgtcactatcatcgctcaggcaccgaagatgttgccgcacattccacaaatgcgcgtgtttattgccgaagatct
cggctgccatatggatgatgttaacgtgaaagccactactacggaaaaactgggatttaccggacgtggggaagggatt
gcctgtgaagcggtggcgctactcattaaggcaacaaaatgatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata