LOCUS BBa_K1653026 4998 bp DNA linear 29-Nov-2024 DEFINITION Geranyl diphosphate (geraniol precursor）production. dev ACCESSION BBa_K1653026 VERSION BBa_K1653026.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..981 /label=m-ispA CDS 82..981 /label=BBa_K1653004 stem_loop 1001..1044 /label=stem_loop misc_feature 990..1069 /label=BBa_B0010 terminator 990..1069 /label=BBa_B0010 stem_loop 1085..1104 /label=T7 TE misc_feature 1111..1111 /label=stop polyA_site 1105..1118 /label=polya misc_feature 1078..1118 /label=BBa_B0012 terminator 1078..1118 /label=BBa_B0012 terminator 990..1118 /label=BBa_B0015 misc_feature 1129..1145 /label=lac O1 promoter 1146..1151 /label=-35 misc_feature 1152..1168 /label=lac O1 promoter 1169..1174 /label=-10 misc_feature 1127..1180 /label=BBa_R0011 promoter 1127..1180 /label=BBa_R0011 misc_feature 1194..1197 /label=conserved RBS 1190..1201 /label=BBa_B0034 CDS 1208..3070 /label=dxs CDS 1208..3070 /label=BBa_K1653000 misc_feature 3083..3086 /label=conserved RBS 3079..3090 /label=BBa_B0034 CDS 3097..3645 /label=idi CDS 3097..3645 /label=BBa_K1653002 misc_feature 3658..3661 /label=conserved RBS 3654..3665 /label=BBa_B0034 CDS 3672..4861 /label=ispDF CDS 3672..4861 /label=BBa_K1653001 stem_loop 4881..4924 /label=stem_loop misc_feature 4870..4949 /label=BBa_B0010 terminator 4870..4949 /label=BBa_B0010 stem_loop 4965..4984 /label=T7 TE misc_feature 4991..4991 /label=stop polyA_site 4985..4998 /label=polya misc_feature 4958..4998 /label=BBa_B0012 terminator 4958..4998 /label=BBa_B0012 terminator 4870..4998 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatggacttt ccgcagcaac tcgaagcctg cgttaagcag 121 gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc 181 gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat 241 gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc tgccgccgtt 301 gagtgtatcc acgcttactt tttaattcat gatgatttac cggcaatgga tgatgacgat 361 ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct 421 ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgccgatat gccggaagtg 481 tcggaccgcg acagaatttc gatgatttct gaactggcga gcgccagtgg tattgccgga 541 atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg 601 cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt 661 gcattaagcg ccggagataa aggacgtcgt gctctgccgg tactcgacaa gtatgcagag 721 agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg agatactgca 781 acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt 841 ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg 901 ctgaaacaac tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac 961 atcatccagc gtaataaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 1021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1081 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt gtgagcggat 1141 aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga aagaggagaa 1201 atactagatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca 1261 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta 1321 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga 1381 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt 1441 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat 1501 ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat atgacgtatt 1561 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttgctgccga 1621 aaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgcagg 1681 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtgattct 1741 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaacaacc atctggcaca 1801 gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag ttttctctgg 1861 cgtgccgcca attaaagagc tgctcaaacg caccgaagaa catattaaag gcatggtagt 1921 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggtgg acggtcacga 1981 tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc cgcagttcct 2041 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc cgatcacttt 2101 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt 2161 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa 2221 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa 2281 attcccggat cgctacttcg acgtggcaat tgccgagcaa cacgcggtga cctttgctgc 2341 gggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg 2401 cgcctatgat caggtgctgc atgacgtggc gattcaaaag cttccggtcc tgttcgccat 2461 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggtgctt ttgatctctc 2521 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg 2581 ccagatgctc tataccggct atcactataa cgatggcccg tcagcggtgc gctacccgcg 2641 tggcaacgcg gtcggcgtgg aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat 2701 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagaagc 2761 ggcgaaagtc gccgaatcgc tgaacgccac gctggtcgat atgcgttttg tgaaaccgct 2821 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga 2881 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctgatgg cccatcgtaa 2941 accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag gaactcagga 3001 agaaatgcgc gccgaactcg gcctcgatgc cgctggtatg gaagccaaaa tcaaggcctg 3061 gctggcataa tactagagaa agaggagaaa tactagatgc aaacggaaca cgtcatttta 3121 ttgaatgcac agggagttcc cacgggtacg ctggaaaagt atgccgcaca cacggcagac 3181 acccgcttac atctcgcgtt ctccagttgg ctgtttaatg ccaaaggaca attattagtt 3241 acccgccgcg cactgagcaa aaaagcatgg cctggcgtgt ggactaactc ggtttgtggg 3301 cacccacaac tgggagaaag caacgaagac gcagtgatcc gccgttgccg ttatgagctt 3361 ggcgtggaaa ttacgcctcc tgaatctatc tatcctgact ttcgctaccg cgccaccgat 3421 ccgagtggca ttgtggaaaa tgaagtgtgt ccggtatttg ccgcacgcac cactagcgcg 3481 ttacagatca atgatgatga agtgatggat tatcaatggt gtgatttagc agatgtatta 3541 cacggtattg atgccacgcc gtgggcgttc agtccgtgga tggtgatgca ggcgacaaat 3601 cgcgaagcca gaaaacgatt atctgcattt acccagctta aataatacta gagaaagagg 3661 agaaatacta gatggcaacc actcatttgg atgtttgcgc cgtggttccg gcggccggat 3721 ttggccgtcg aatgcaaacg gaatgtccta agcaatatct ctcaatcggt aatcaaacca 3781 ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg ggtgaaacgt gtcgtcattg 3841 ccataagtcc tggcgatagc cgttttgcac aacttcctct ggcgaatcat ccgcaaatca 3901 ccgttgtaga tggcggtgat gagcgtgccg attccgtgct ggcaggtctg aaagccgctg 3961 gcgacgcgca gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat caggatgacc 4021 tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg ggggatcctc gccgcaccag 4081 tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat accgttgatc 4141 gcaacggctt atggcacgcg ctgacgccgc aatttttccc tcgtgagctg ttacatgact 4201 gtctgacgcg cgctctaaat gaaggcgcga ctattaccga cgaagcctcg gcgctggaat 4261 attgcggatt ccatcctcag ttggtcgaag gccgtgcgga taacattaaa gtcacgcgcc 4321 cggaagattt ggcactggcc gagttttacc tcacccgaac catccatcag gagaatacat 4381 aatgcgaatt ggacacggtt ttgacgtaca tgcctttggc ggtgaaggcc caattatcat 4441 tggtggcgta cgcattcctt acgaaaaagg attgctggcg cattctgatg gcgacgtggc 4501 gctccatgcg ttgaccgatg cattgcttgg cgcggcggcg ctgggggata tcggcaagct 4561 gttcccggat accgatccgg catttaaagg tgccgatagc cgcgagctgc tacgcgaagc 4621 ctggcgtcgt attcaggcga agggttatac ccttggcaac gtcgatgtca ctatcatcgc 4681 tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc gtgtttattg ccgaagatct 4741 cggctgccat atggatgatg ttaacgtgaa agccactact acggaaaaac tgggatttac 4801 cggacgtggg gaagggattg cctgtgaagc ggtggcgcta ctcattaagg caacaaaatg 4861 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 4921 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 4981 ggcctttctg cgtttata //