LOCUS BBa_K1653027 6928 bp DNA linear 23-Nov-2024 DEFINITION Geraniol(GOH) production. dev ACCESSION BBa_K1653027 VERSION BBa_K1653027.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..1791 /label=ObGES CDS 82..1785 /label=BBa_K1653007 stem_loop 1805..1848 /label=stem_loop misc_feature 1794..1873 /label=BBa_B0010 terminator 1794..1873 /label=BBa_B0010 stem_loop 1889..1908 /label=T7 TE misc_feature 1915..1915 /label=stop polyA_site 1909..1922 /label=polya misc_feature 1882..1922 /label=BBa_B0012 terminator 1882..1922 /label=BBa_B0012 terminator 1794..1922 /label=BBa_B0015 misc_feature 1933..1949 /label=lac O1 promoter 1950..1955 /label=-35 misc_feature 1956..1972 /label=lac O1 promoter 1973..1978 /label=-10 misc_feature 1931..1984 /label=BBa_R0011 promoter 1931..1984 /label=BBa_R0011 misc_feature 1998..2001 /label=conserved RBS 1994..2005 /label=BBa_B0034 CDS 2012..2911 /label=m-ispA CDS 2012..2911 /label=BBa_K1653004 stem_loop 2931..2974 /label=stem_loop misc_feature 2920..2999 /label=BBa_B0010 terminator 2920..2999 /label=BBa_B0010 stem_loop 3015..3034 /label=T7 TE misc_feature 3041..3041 /label=stop polyA_site 3035..3048 /label=polya misc_feature 3008..3048 /label=BBa_B0012 terminator 3008..3048 /label=BBa_B0012 terminator 2920..3048 /label=BBa_B0015 misc_feature 3059..3075 /label=lac O1 promoter 3076..3081 /label=-35 misc_feature 3082..3098 /label=lac O1 promoter 3099..3104 /label=-10 misc_feature 3057..3110 /label=BBa_R0011 promoter 3057..3110 /label=BBa_R0011 misc_feature 3124..3127 /label=conserved RBS 3120..3131 /label=BBa_B0034 CDS 3138..5000 /label=dxs CDS 3138..5000 /label=BBa_K1653000 misc_feature 5013..5016 /label=conserved RBS 5009..5020 /label=BBa_B0034 CDS 5027..5575 /label=idi CDS 5027..5575 /label=BBa_K1653002 misc_feature 5588..5591 /label=conserved RBS 5584..5595 /label=BBa_B0034 CDS 5602..6791 /label=ispDF CDS 5602..6791 /label=BBa_K1653001 stem_loop 6811..6854 /label=stem_loop misc_feature 6800..6879 /label=BBa_B0010 terminator 6800..6879 /label=BBa_B0010 stem_loop 6895..6914 /label=T7 TE misc_feature 6921..6921 /label=stop polyA_site 6915..6928 /label=polya misc_feature 6888..6928 /label=BBa_B0012 terminator 6888..6928 /label=BBa_B0012 terminator 6800..6928 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgtcttgt gctcgtatta ccgttactct gccttatcgt 121 tctgctaaaa ctagcattca acgcggtatc acccactatc cggcgctgat tcgtccacgt 181 tttagcgcgt gcactccgct ggcgagcgct atgccgctgt cctccacgcc gctgatcaac 241 ggcgacaaca gccagcgtaa aaacacccgt cagcacatgg aagagtcttc ttctaagcgt 301 cgcgaatacc tgctggaaga gactacccgt aaactgcaac gtaacgacac tgaatctgta 361 gagaagctga aactgatcga caacatccag cagctgggca tcggttacta tttcgaggat 421 gcgattaacg ctgtcctgcg ttctccgttc tctactggtg aagaggacct gtttactgcg 481 gccctgcgtt tccgtctgct gcgtcacaac ggcatcgaaa tttccccgga aatcttcctg 541 aagttcaagg acgaacgcgg caaattcgac gaatccgata ctctgggtct gctgagcctg 601 tacgaagcca gcaacctggg tgttgcgggc gaggaaatcc tggaagaagc aatggagttc 661 gcggaagcgc gcctgcgtcg ctccctgagc gaacctgctg cacctctgca cggcgaagtt 721 gcacaagcac tggacgttcc gcgtcacctg cgcatggctc gtctggaagc acgtcgtttc 781 atcgaacaat atggtaaaca atccgaccac gatggcgacc tgctggagct ggcaatcctg 841 gattataatc aagtccaggc gcagcaccag tctgagctga ccgagatcat ccgttggtgg 901 aaagaactgg gcctggttga taaactgtct tttggtcgtg atcgtccgct ggaatgtttc 961 ctgtggactg taggtctgct gccagagcct aaatactctt ccgtccgtat cgaactggct 1021 aaggctattt ctatcctgct ggtcattgat gatatcttcg acacctacgg cgagatggat 1081 gatctgatcc tgttcaccga cgctatccgt cgttgggacc tggaagcgat ggaaggtctg 1141 ccggaataca tgaagatttg ctacatggcc ctgtataaca ctaccaacga ggtttgttac 1201 aaagtactgc gtgacacggg ccgtatcgtt ctgctgaatc tgaagtccac ctggattgat 1261 atgatcgagg gtttcatgga ggaagcgaag tggttcaacg gtggcagcgc cccgaaactg 1321 gaagaataca tcgaaaacgg tgtgtccacc gctggtgctt acatggcctt tgctcatatc 1381 ttcttcctga ttggtgaagg tgtaacgcac caaaactctc aactgtttac ccagaaacct 1441 tatccgaaag tgtttagcgc agccggccgt attctgcgtc tgtgggacga tctgggcacc 1501 gcgaaggaag aacaagaacg cggtgacctg gcaagctgcg ttcagctgtt catgaaagag 1561 aaatccctga ccgaggaaga agcacgttcc cgcattctgg aggagattaa aggcctgtgg 1621 cgtgacctga acggtgaact ggtttataac aaaaatctgc cgctgtccat tatcaaggtg 1681 gccctgaaca tggcacgcgc atcccaggta gtttataaac atgaccagga tacctatttc 1741 tcctccgtag ataactacgt tgacgccctg ttcttcactc aatgatacta gagccaggca 1801 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1861 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1921 tatactagag aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga 1981 gcacatacta gagaaagagg agaaatacta gatggacttt ccgcagcaac tcgaagcctg 2041 cgttaagcag gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac 2101 tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt 2161 cctggtttat gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc 2221 tgccgccgtt gagtgtatcc acgcttactt tttaattcat gatgatttac cggcaatgga 2281 tgatgacgat ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg aagcaaacgc 2341 gattctcgct ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgccgatat 2401 gccggaagtg tcggaccgcg acagaatttc gatgatttct gaactggcga gcgccagtgg 2461 tattgccgga atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc 2521 tctggacgcg cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt 2581 tcgccttggt gcattaagcg ccggagataa aggacgtcgt gctctgccgg tactcgacaa 2641 gtatgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg 2701 agatactgca acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta 2761 ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc 2821 ccgtcagtcg ctgaaacaac tggctgaaca gtcactcgat acctcggcac tggaagcgct 2881 agcggactac atcatccagc gtaataaata atactagagc caggcatcaa ataaaacgaa 2941 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3001 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 3061 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga 3121 aagaggagaa atactagatg agttttgata ttgccaaata cccgaccctg gcactggtcg 3181 actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac 3241 tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg 3301 gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga 3361 tttgggatgt ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa 3421 tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat 3481 atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg 3541 ttgctgccga aaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga 3601 ttaccgcagg catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc 3661 tggtgattct caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaacaacc 3721 atctggcaca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag 3781 ttttctctgg cgtgccgcca attaaagagc tgctcaaacg caccgaagaa catattaaag 3841 gcatggtagt gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggtgg 3901 acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc 3961 cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc 4021 cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta 4081 gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga 4141 aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt 4201 tttcacgtaa attcccggat cgctacttcg acgtggcaat tgccgagcaa cacgcggtga 4261 cctttgctgc gggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt 4321 tcctgcaacg cgcctatgat caggtgctgc atgacgtggc gattcaaaag cttccggtcc 4381 tgttcgccat cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggtgctt 4441 ttgatctctc ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa 4501 acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg tcagcggtgc 4561 gctacccgcg tggcaacgcg gtcggcgtgg aactgacgcc gctggaaaaa ctaccaattg 4621 gcaaaggcat tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga 4681 tgccagaagc ggcgaaagtc gccgaatcgc tgaacgccac gctggtcgat atgcgttttg 4741 tgaaaccgct tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca 4801 ccgtagaaga aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctgatgg 4861 cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag 4921 gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgctggtatg gaagccaaaa 4981 tcaaggcctg gctggcataa tactagagaa agaggagaaa tactagatgc aaacggaaca 5041 cgtcatttta ttgaatgcac agggagttcc cacgggtacg ctggaaaagt atgccgcaca 5101 cacggcagac acccgcttac atctcgcgtt ctccagttgg ctgtttaatg ccaaaggaca 5161 attattagtt acccgccgcg cactgagcaa aaaagcatgg cctggcgtgt ggactaactc 5221 ggtttgtggg cacccacaac tgggagaaag caacgaagac gcagtgatcc gccgttgccg 5281 ttatgagctt ggcgtggaaa ttacgcctcc tgaatctatc tatcctgact ttcgctaccg 5341 cgccaccgat ccgagtggca ttgtggaaaa tgaagtgtgt ccggtatttg ccgcacgcac 5401 cactagcgcg ttacagatca atgatgatga agtgatggat tatcaatggt gtgatttagc 5461 agatgtatta cacggtattg atgccacgcc gtgggcgttc agtccgtgga tggtgatgca 5521 ggcgacaaat cgcgaagcca gaaaacgatt atctgcattt acccagctta aataatacta 5581 gagaaagagg agaaatacta gatggcaacc actcatttgg atgtttgcgc cgtggttccg 5641 gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct ctcaatcggt 5701 aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg ggtgaaacgt 5761 gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct ggcgaatcat 5821 ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct ggcaggtctg 5881 aaagccgctg gcgacgcgca gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat 5941 caggatgacc tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg ggggatcctc 6001 gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat 6061 accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc tcgtgagctg 6121 ttacatgact gtctgacgcg cgctctaaat gaaggcgcga ctattaccga cgaagcctcg 6181 gcgctggaat attgcggatt ccatcctcag ttggtcgaag gccgtgcgga taacattaaa 6241 gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac catccatcag 6301 gagaatacat aatgcgaatt ggacacggtt ttgacgtaca tgcctttggc ggtgaaggcc 6361 caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg cattctgatg 6421 gcgacgtggc gctccatgcg ttgaccgatg cattgcttgg cgcggcggcg ctgggggata 6481 tcggcaagct gttcccggat accgatccgg catttaaagg tgccgatagc cgcgagctgc 6541 tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac gtcgatgtca 6601 ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc gtgtttattg 6661 ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact acggaaaaac 6721 tgggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta ctcattaagg 6781 caacaaaatg atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 6841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 6901 ccttcgggtg ggcctttctg cgtttata //