##gff-version 3
##sequence-region BBa_K1653027 1 6928
BBa_K1653027	.	CDS	3138	5000	.	+	0	ID=BBa_K1653000;Name=BBa_K1653000
BBa_K1653027	.	CDS	3138	5000	.	+	0	ID=annotation2432264;Name=dxs;Parent=BBa_K1653000
BBa_K1653027	.	CDS	2012	2911	.	+	0	ID=BBa_K1653004;Name=BBa_K1653004
BBa_K1653027	.	CDS	2012	2911	.	+	0	ID=annotation2445843;Name=m-ispA;Parent=BBa_K1653004
BBa_K1653027	.	CDS	5602	6791	.	+	0	ID=BBa_K1653001;Name=BBa_K1653001
BBa_K1653027	.	CDS	5602	6791	.	+	0	ID=annotation2445940;Name=ispDF;Parent=BBa_K1653001
BBa_K1653027	.	terminator	1794	1922	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653027	.	terminator	1794	1873	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653027	.	engineered_region	1794	1873	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653027	.	stem_loop	1805	1848	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653027	.	terminator	1882	1922	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653027	.	stop_codon	1915	1915	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653027	.	polyA_site	1909	1922	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653027	.	stem_loop	1889	1908	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653027	.	engineered_region	1882	1922	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653027	.	promoter	1931	1984	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653027	.	promoter	1973	1978	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653027	.	engineered_region	1931	1984	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653027	.	operator	1933	1949	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653027	.	promoter	1950	1955	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653027	.	operator	1956	1972	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653027	.	ribosome_entry_site	1994	2005	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653027	.	conserved_region	1998	2001	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653027	.	CDS	5027	5575	.	+	0	ID=BBa_K1653002;Name=BBa_K1653002
BBa_K1653027	.	CDS	5027	5575	.	+	0	ID=annotation2432275;Name=idi;Parent=BBa_K1653002
BBa_K1653027	.	terminator	6800	6928	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653027	.	terminator	6800	6879	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653027	.	engineered_region	6800	6879	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653027	.	stem_loop	6811	6854	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653027	.	terminator	6888	6928	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653027	.	stop_codon	6921	6921	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653027	.	polyA_site	6915	6928	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653027	.	stem_loop	6895	6914	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653027	.	engineered_region	6888	6928	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653027	.	ribosome_entry_site	3120	3131	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653027	.	conserved_region	3124	3127	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653027	.	terminator	2920	3048	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653027	.	terminator	2920	2999	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653027	.	engineered_region	2920	2999	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653027	.	stem_loop	2931	2974	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653027	.	terminator	3008	3048	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653027	.	stop_codon	3041	3041	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653027	.	polyA_site	3035	3048	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653027	.	stem_loop	3015	3034	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653027	.	engineered_region	3008	3048	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653027	.	promoter	3057	3110	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653027	.	promoter	3099	3104	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653027	.	engineered_region	3057	3110	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653027	.	operator	3059	3075	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653027	.	promoter	3076	3081	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653027	.	operator	3082	3098	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653027	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653027	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653027	.	ribosome_entry_site	5584	5595	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653027	.	conserved_region	5588	5591	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653027	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653027	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653027	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653027	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653027	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653027	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653027	.	CDS	82	1785	.	+	0	ID=BBa_K1653007;Name=BBa_K1653007
BBa_K1653027	.	CDS	82	1791	.	+	0	ID=annotation2446618;Name=ObGES;Parent=BBa_K1653007
BBa_K1653027	.	ribosome_entry_site	5009	5020	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653027	.	conserved_region	5013	5016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1653027
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgtcttgtgctcgtattaccgttactctgccttatcgttctgctaaaactagcattcaacgcggtatcacccacta
tccggcgctgattcgtccacgttttagcgcgtgcactccgctggcgagcgctatgccgctgtcctccacgccgctgatc
aacggcgacaacagccagcgtaaaaacacccgtcagcacatggaagagtcttcttctaagcgtcgcgaatacctgctgg
aagagactacccgtaaactgcaacgtaacgacactgaatctgtagagaagctgaaactgatcgacaacatccagcagct
gggcatcggttactatttcgaggatgcgattaacgctgtcctgcgttctccgttctctactggtgaagaggacctgttt
actgcggccctgcgtttccgtctgctgcgtcacaacggcatcgaaatttccccggaaatcttcctgaagttcaaggacg
aacgcggcaaattcgacgaatccgatactctgggtctgctgagcctgtacgaagccagcaacctgggtgttgcgggcga
ggaaatcctggaagaagcaatggagttcgcggaagcgcgcctgcgtcgctccctgagcgaacctgctgcacctctgcac
ggcgaagttgcacaagcactggacgttccgcgtcacctgcgcatggctcgtctggaagcacgtcgtttcatcgaacaat
atggtaaacaatccgaccacgatggcgacctgctggagctggcaatcctggattataatcaagtccaggcgcagcacca
gtctgagctgaccgagatcatccgttggtggaaagaactgggcctggttgataaactgtcttttggtcgtgatcgtccg
ctggaatgtttcctgtggactgtaggtctgctgccagagcctaaatactcttccgtccgtatcgaactggctaaggcta
tttctatcctgctggtcattgatgatatcttcgacacctacggcgagatggatgatctgatcctgttcaccgacgctat
ccgtcgttgggacctggaagcgatggaaggtctgccggaatacatgaagatttgctacatggccctgtataacactacc
aacgaggtttgttacaaagtactgcgtgacacgggccgtatcgttctgctgaatctgaagtccacctggattgatatga
tcgagggtttcatggaggaagcgaagtggttcaacggtggcagcgccccgaaactggaagaatacatcgaaaacggtgt
gtccaccgctggtgcttacatggcctttgctcatatcttcttcctgattggtgaaggtgtaacgcaccaaaactctcaa
ctgtttacccagaaaccttatccgaaagtgtttagcgcagccggccgtattctgcgtctgtgggacgatctgggcaccg
cgaaggaagaacaagaacgcggtgacctggcaagctgcgttcagctgttcatgaaagagaaatccctgaccgaggaaga
agcacgttcccgcattctggaggagattaaaggcctgtggcgtgacctgaacggtgaactggtttataacaaaaatctg
ccgctgtccattatcaaggtggccctgaacatggcacgcgcatcccaggtagtttataaacatgaccaggatacctatt
tctcctccgtagataactacgttgacgccctgttcttcactcaatgatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagat
actgagcacatactagagaaagaggagaaatactagatggactttccgcagcaactcgaagcctgcgttaagcaggcca
accaggcgctgagccgttttatcgccccactgccctttcagaacactcccgtggtcgaaaccatgcagtatggcgcatt
attaggtggtaagcgcctgcgacctttcctggtttatgccaccggtcatatgttcggcgttagcacaaacacgctggac
gcacccgctgccgccgttgagtgtatccacgcttactttttaattcatgatgatttaccggcaatggatgatgacgatc
tgcgtcgcggtttgccaacctgccatgtgaagtttggcgaagcaaacgcgattctcgctggcgacgctttacaaacgct
ggcgttctcgattttaagcgatgccgatatgccggaagtgtcggaccgcgacagaatttcgatgatttctgaactggcg
agcgccagtggtattgccggaatgtgcggtggtcaggcattagatttagacgcggaaggcaaacacgtacctctggacg
cgcttgagcgtattcatcgtcataaaaccggcgcattgattcgcgccgccgttcgccttggtgcattaagcgccggaga
taaaggacgtcgtgctctgccggtactcgacaagtatgcagagagcatcggccttgccttccaggttcaggatgacatc
ctggatgtggtgggagatactgcaacgttgggaaaacgccagggtgccgaccagcaacttggtaaaagtacctaccctg
cacttctgggtcttgagcaagcccggaagaaagcccgggatctgatcgacgatgcccgtcagtcgctgaaacaactggc
tgaacagtcactcgatacctcggcactggaagcgctagcggactacatcatccagcgtaataaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgaca
ttgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagttttgatattgccaaata
cccgaccctggcactggtcgactccacccaggagttacgactgttgccgaaagagagtttaccgaaactctgcgacgaa
ctgcgccgctatttactcgacagcgtgagccgttccagcgggcacttcgcctccgggctgggcacggtcgaactgaccg
tggcgctgcactatgtctacaacaccccgtttgaccaattgatttgggatgtggggcatcaggcttatccgcataaaat
tttgaccggacgccgcgacaaaatcggcaccatccgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagc
gaatatgacgtattaagcgtcgggcattcatcaacctccatcagtgccggaattggtattgcggttgctgccgaaaaag
aaggcaaaaatcgccgcaccgtctgtgtcattggcgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatca
cgcgggcgatatccgtcctgatatgctggtgattctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctc
aacaaccatctggcacagctgctttccggtaagctttactcttcactgcgcgaaggcgggaaaaaagttttctctggcg
tgccgccaattaaagagctgctcaaacgcaccgaagaacatattaaaggcatggtagtgcctggcacgttgtttgaaga
gctgggctttaactacatcggcccggtggacggtcacgatgtgctggggcttatcaccacgctaaagaacatgcgcgac
ctgaaaggcccgcagttcctgcatatcatgaccaaaaaaggtcgtggttatgaaccggcagaaaaagacccgatcactt
tccacgccgtgcctaaatttgatccctccagcggttgtttgccgaaaagtagcggcggtttgccgagctattcaaaaat
ctttggcgactggttgtgcgaaacggcagcgaaagacaacaagctgatggcgattactccggcgatgcgtgaaggttcc
ggcatggtcgagttttcacgtaaattcccggatcgctacttcgacgtggcaattgccgagcaacacgcggtgacctttg
ctgcgggtctggcgattggtgggtacaaacccattgtcgcgatttactccactttcctgcaacgcgcctatgatcaggt
gctgcatgacgtggcgattcaaaagcttccggtcctgttcgccatcgaccgcgcgggcattgttggtgctgacggtcaa
acccatcagggtgcttttgatctctcttacctgcgctgcataccggaaatggtcattatgaccccgagcgatgaaaacg
aatgtcgccagatgctctataccggctatcactataacgatggcccgtcagcggtgcgctacccgcgtggcaacgcggt
cggcgtggaactgacgccgctggaaaaactaccaattggcaaaggcattgtgaagcgtcgtggcgagaaactggcgatc
cttaactttggtacgctgatgccagaagcggcgaaagtcgccgaatcgctgaacgccacgctggtcgatatgcgttttg
tgaaaccgcttgatgaagcgttaattctggaaatggccgccagccatgaagcgctggtcaccgtagaagaaaacgccat
tatgggcggcgcaggcagcggcgtgaacgaagtgctgatggcccatcgtaaaccagtacccgtgctgaacattggcctg
ccggacttctttattccgcaaggaactcaggaagaaatgcgcgccgaactcggcctcgatgccgctggtatggaagcca
aaatcaaggcctggctggcataatactagagaaagaggagaaatactagatgcaaacggaacacgtcattttattgaat
gcacagggagttcccacgggtacgctggaaaagtatgccgcacacacggcagacacccgcttacatctcgcgttctcca
gttggctgtttaatgccaaaggacaattattagttacccgccgcgcactgagcaaaaaagcatggcctggcgtgtggac
taactcggtttgtgggcacccacaactgggagaaagcaacgaagacgcagtgatccgccgttgccgttatgagcttggc
gtggaaattacgcctcctgaatctatctatcctgactttcgctaccgcgccaccgatccgagtggcattgtggaaaatg
aagtgtgtccggtatttgccgcacgcaccactagcgcgttacagatcaatgatgatgaagtgatggattatcaatggtg
tgatttagcagatgtattacacggtattgatgccacgccgtgggcgttcagtccgtggatggtgatgcaggcgacaaat
cgcgaagccagaaaacgattatctgcatttacccagcttaaataatactagagaaagaggagaaatactagatggcaac
cactcatttggatgtttgcgccgtggttccggcggccggatttggccgtcgaatgcaaacggaatgtcctaagcaatat
ctctcaatcggtaatcaaaccattcttgaacactcggtgcatgcgctgctggcgcatccccgggtgaaacgtgtcgtca
ttgccataagtcctggcgatagccgttttgcacaacttcctctggcgaatcatccgcaaatcaccgttgtagatggcgg
tgatgagcgtgccgattccgtgctggcaggtctgaaagccgctggcgacgcgcagtgggtattggtgcatgacgccgct
cgtccttgtttgcatcaggatgacctcgcgcgattgttggcgttgagcgaaaccagccgcacgggggggatcctcgccg
caccagtgcgcgatactatgaaacgtgccgaaccgggcaaaaatgccattgctcataccgttgatcgcaacggcttatg
gcacgcgctgacgccgcaatttttccctcgtgagctgttacatgactgtctgacgcgcgctctaaatgaaggcgcgact
attaccgacgaagcctcggcgctggaatattgcggattccatcctcagttggtcgaaggccgtgcggataacattaaag
tcacgcgcccggaagatttggcactggccgagttttacctcacccgaaccatccatcaggagaatacataatgcgaatt
ggacacggttttgacgtacatgcctttggcggtgaaggcccaattatcattggtggcgtacgcattccttacgaaaaag
gattgctggcgcattctgatggcgacgtggcgctccatgcgttgaccgatgcattgcttggcgcggcggcgctggggga
tatcggcaagctgttcccggataccgatccggcatttaaaggtgccgatagccgcgagctgctacgcgaagcctggcgt
cgtattcaggcgaagggttatacccttggcaacgtcgatgtcactatcatcgctcaggcaccgaagatgttgccgcaca
ttccacaaatgcgcgtgtttattgccgaagatctcggctgccatatggatgatgttaacgtgaaagccactactacgga
aaaactgggatttaccggacgtggggaagggattgcctgtgaagcggtggcgctactcattaaggcaacaaaatgatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata