LOCUS BBa_K1653028 5677 bp DNA linear 26-Nov-2024 DEFINITION RhNADX1+ispA+dxs+m-idi+ispDF.dev ACCESSION BBa_K1653028 VERSION BBa_K1653028.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..547 /label=RhNUDX1 CDS 82..534 /label=BBa_K1653008 stem_loop 554..597 /label=stem_loop misc_feature 543..622 /label=BBa_B0010 terminator 543..622 /label=BBa_B0010 stem_loop 638..657 /label=T7 TE misc_feature 664..664 /label=stop polyA_site 658..671 /label=polya misc_feature 631..671 /label=BBa_B0012 terminator 631..671 /label=BBa_B0012 terminator 543..671 /label=BBa_B0015 misc_feature 682..698 /label=lac O1 promoter 699..704 /label=-35 misc_feature 705..721 /label=lac O1 promoter 722..727 /label=-10 misc_feature 680..733 /label=BBa_R0011 promoter 680..733 /label=BBa_R0011 misc_feature 747..750 /label=conserved RBS 743..754 /label=BBa_B0034 CDS 761..1660 /label=m-ispA CDS 761..1660 /label=BBa_K1653004 stem_loop 1680..1723 /label=stem_loop misc_feature 1669..1748 /label=BBa_B0010 terminator 1669..1748 /label=BBa_B0010 stem_loop 1764..1783 /label=T7 TE misc_feature 1790..1790 /label=stop polyA_site 1784..1797 /label=polya misc_feature 1757..1797 /label=BBa_B0012 terminator 1757..1797 /label=BBa_B0012 terminator 1669..1797 /label=BBa_B0015 misc_feature 1808..1824 /label=lac O1 promoter 1825..1830 /label=-35 misc_feature 1831..1847 /label=lac O1 promoter 1848..1853 /label=-10 misc_feature 1806..1859 /label=BBa_R0011 promoter 1806..1859 /label=BBa_R0011 misc_feature 1873..1876 /label=conserved RBS 1869..1880 /label=BBa_B0034 CDS 1887..3749 /label=dxs CDS 1887..3749 /label=BBa_K1653000 misc_feature 3762..3765 /label=conserved RBS 3758..3769 /label=BBa_B0034 CDS 3776..4324 /label=idi CDS 3776..4324 /label=BBa_K1653002 misc_feature 4337..4340 /label=conserved RBS 4333..4344 /label=BBa_B0034 CDS 4351..5540 /label=ispDF CDS 4351..5540 /label=BBa_K1653001 stem_loop 5560..5603 /label=stem_loop misc_feature 5549..5628 /label=BBa_B0010 terminator 5549..5628 /label=BBa_B0010 stem_loop 5644..5663 /label=T7 TE misc_feature 5670..5670 /label=stop polyA_site 5664..5677 /label=polya misc_feature 5637..5677 /label=BBa_B0012 terminator 5637..5677 /label=BBa_B0012 terminator 5549..5677 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgggtaat gaaacggtgg tcgtggctga aacggctggt 121 tcgatcaaag ttgcggtcgt ggtctgcctg ctgcgtggtc aaaacgtgct gctgggtcgt 181 cgccgtagca gcctgggtga tagcaccttt agcctgccga gcggccatct ggaatttggt 241 gaaagctttg aagaatgcgc ggcccgcgaa ctgaaagaag aaaccgatct ggatattggc 301 aaaatcgaac tgctgaccgt gaccaacaac ctgtttctgg atgaagcgaa accgagccag 361 tatgtggccg tttttatgcg tgccgttctg gcggatccgc gtcaggaacc gcagaacatt 421 gaaccggaat tttgtgatgg ctggggttgg tatgaatggg ataatctgcc gaaaccgctg 481 ttctggccgc tggacaatgt ggttcaagac ggttttaatc cgttcccgac ctaatactag 541 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 601 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 661 ctgcgtttat atactagaga attgtgagcg gataacaatt gacattgtga gcggataaca 721 agatactgag cacatactag agaaagagga gaaatactag atggactttc cgcagcaact 781 cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt tttatcgccc cactgccctt 841 tcagaacact cccgtggtcg aaaccatgca gtatggcgca ttattaggtg gtaagcgcct 901 gcgacctttc ctggtttatg ccaccggtca tatgttcggc gttagcacaa acacgctgga 961 cgcacccgct gccgccgttg agtgtatcca cgcttacttt ttaattcatg atgatttacc 1021 ggcaatggat gatgacgatc tgcgtcgcgg tttgccaacc tgccatgtga agtttggcga 1081 agcaaacgcg attctcgctg gcgacgcttt acaaacgctg gcgttctcga ttttaagcga 1141 tgccgatatg ccggaagtgt cggaccgcga cagaatttcg atgatttctg aactggcgag 1201 cgccagtggt attgccggaa tgtgcggtgg tcaggcatta gatttagacg cggaaggcaa 1261 acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat aaaaccggcg cattgattcg 1321 cgccgccgtt cgccttggtg cattaagcgc cggagataaa ggacgtcgtg ctctgccggt 1381 actcgacaag tatgcagaga gcatcggcct tgccttccag gttcaggatg acatcctgga 1441 tgtggtggga gatactgcaa cgttgggaaa acgccagggt gccgaccagc aacttggtaa 1501 aagtacctac cctgcacttc tgggtcttga gcaagcccgg aagaaagccc gggatctgat 1561 cgacgatgcc cgtcagtcgc tgaaacaact ggctgaacag tcactcgata cctcggcact 1621 ggaagcgcta gcggactaca tcatccagcg taataaataa tactagagcc aggcatcaaa 1681 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1741 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 1801 tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca 1861 tactagagaa agaggagaaa tactagatga gttttgatat tgccaaatac ccgaccctgg 1921 cactggtcga ctccacccag gagttacgac tgttgccgaa agagagttta ccgaaactct 1981 gcgacgaact gcgccgctat ttactcgaca gcgtgagccg ttccagcggg cacttcgcct 2041 ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac accccgtttg 2101 accaattgat ttgggatgtg gggcatcagg cttatccgca taaaattttg accggacgcc 2161 gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca cccgttcccg tggcgcggcg 2221 aaagcgaata tgacgtatta agcgtcgggc attcatcaac ctccatcagt gccggaattg 2281 gtattgcggt tgctgccgaa aaagaaggca aaaatcgccg caccgtctgt gtcattggcg 2341 atggcgcgat taccgcaggc atggcgtttg aagcgatgaa tcacgcgggc gatatccgtc 2401 ctgatatgct ggtgattctc aacgacaatg aaatgtcgat ttccgaaaat gtcggcgcgc 2461 tcaacaacca tctggcacag ctgctttccg gtaagcttta ctcttcactg cgcgaaggcg 2521 ggaaaaaagt tttctctggc gtgccgccaa ttaaagagct gctcaaacgc accgaagaac 2581 atattaaagg catggtagtg cctggcacgt tgtttgaaga gctgggcttt aactacatcg 2641 gcccggtgga cggtcacgat gtgctggggc ttatcaccac gctaaagaac atgcgcgacc 2701 tgaaaggccc gcagttcctg catatcatga ccaaaaaagg tcgtggttat gaaccggcag 2761 aaaaagaccc gatcactttc cacgccgtgc ctaaatttga tccctccagc ggttgtttgc 2821 cgaaaagtag cggcggtttg ccgagctatt caaaaatctt tggcgactgg ttgtgcgaaa 2881 cggcagcgaa agacaacaag ctgatggcga ttactccggc gatgcgtgaa ggttccggca 2941 tggtcgagtt ttcacgtaaa ttcccggatc gctacttcga cgtggcaatt gccgagcaac 3001 acgcggtgac ctttgctgcg ggtctggcga ttggtgggta caaacccatt gtcgcgattt 3061 actccacttt cctgcaacgc gcctatgatc aggtgctgca tgacgtggcg attcaaaagc 3121 ttccggtcct gttcgccatc gaccgcgcgg gcattgttgg tgctgacggt caaacccatc 3181 agggtgcttt tgatctctct tacctgcgct gcataccgga aatggtcatt atgaccccga 3241 gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta tcactataac gatggcccgt 3301 cagcggtgcg ctacccgcgt ggcaacgcgg tcggcgtgga actgacgccg ctggaaaaac 3361 taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa actggcgatc cttaactttg 3421 gtacgctgat gccagaagcg gcgaaagtcg ccgaatcgct gaacgccacg ctggtcgata 3481 tgcgttttgt gaaaccgctt gatgaagcgt taattctgga aatggccgcc agccatgaag 3541 cgctggtcac cgtagaagaa aacgccatta tgggcggcgc aggcagcggc gtgaacgaag 3601 tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat tggcctgccg gacttcttta 3661 ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc gctggtatgg 3721 aagccaaaat caaggcctgg ctggcataat actagagaaa gaggagaaat actagatgca 3781 aacggaacac gtcattttat tgaatgcaca gggagttccc acgggtacgc tggaaaagta 3841 tgccgcacac acggcagaca cccgcttaca tctcgcgttc tccagttggc tgtttaatgc 3901 caaaggacaa ttattagtta cccgccgcgc actgagcaaa aaagcatggc ctggcgtgtg 3961 gactaactcg gtttgtgggc acccacaact gggagaaagc aacgaagacg cagtgatccg 4021 ccgttgccgt tatgagcttg gcgtggaaat tacgcctcct gaatctatct atcctgactt 4081 tcgctaccgc gccaccgatc cgagtggcat tgtggaaaat gaagtgtgtc cggtatttgc 4141 cgcacgcacc actagcgcgt tacagatcaa tgatgatgaa gtgatggatt atcaatggtg 4201 tgatttagca gatgtattac acggtattga tgccacgccg tgggcgttca gtccgtggat 4261 ggtgatgcag gcgacaaatc gcgaagccag aaaacgatta tctgcattta cccagcttaa 4321 ataatactag agaaagagga gaaatactag atggcaacca ctcatttgga tgtttgcgcc 4381 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc 4441 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg 4501 gtgaaacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg 4561 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg 4621 gcaggtctga aagccgctgg cgacgcgcag tgggtattgg tgcatgacgc cgctcgtcct 4681 tgtttgcatc aggatgacct cgcgcgattg ttggcgttga gcgaaaccag ccgcacgggg 4741 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc 4801 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct 4861 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgcgac tattaccgac 4921 gaagcctcgg cgctggaata ttgcggattc catcctcagt tggtcgaagg ccgtgcggat 4981 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc 5041 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacat gcctttggcg 5101 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc 5161 attctgatgg cgacgtggcg ctccatgcgt tgaccgatgc attgcttggc gcggcggcgc 5221 tgggggatat cggcaagctg ttcccggata ccgatccggc atttaaaggt gccgatagcc 5281 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg 5341 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg 5401 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta 5461 cggaaaaact gggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac 5521 tcattaaggc aacaaaatga tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 5581 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 5641 actggctcac cttcgggtgg gcctttctgc gtttata //