LOCUS BBa_K1653029 5326 bp DNA linear 22-Dec-2024 DEFINITION RhNADX1+m-ispA+dxs+m-idi+ispDF.dev ACCESSION BBa_K1653029 VERSION BBa_K1653029.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..547 /label=RhNUDX1 CDS 82..534 /label=BBa_K1653008 stem_loop 554..597 /label=stem_loop misc_feature 543..622 /label=BBa_B0010 terminator 543..622 /label=BBa_B0010 stem_loop 638..657 /label=T7 TE misc_feature 664..664 /label=stop polyA_site 658..671 /label=polya misc_feature 631..671 /label=BBa_B0012 terminator 631..671 /label=BBa_B0012 terminator 543..671 /label=BBa_B0015 misc_feature 682..698 /label=lac O1 promoter 699..704 /label=-35 misc_feature 705..721 /label=lac O1 promoter 722..727 /label=-10 misc_feature 680..733 /label=BBa_R0011 promoter 680..733 /label=BBa_R0011 misc_feature 747..750 /label=conserved RBS 743..754 /label=BBa_B0034 CDS 761..1309 /label=idi CDS 761..1309 /label=BBa_K1653002 stem_loop 1329..1372 /label=stem_loop misc_feature 1318..1397 /label=BBa_B0010 terminator 1318..1397 /label=BBa_B0010 stem_loop 1413..1432 /label=T7 TE misc_feature 1439..1439 /label=stop polyA_site 1433..1446 /label=polya misc_feature 1406..1446 /label=BBa_B0012 terminator 1406..1446 /label=BBa_B0012 terminator 1318..1446 /label=BBa_B0015 misc_feature 1457..1473 /label=lac O1 promoter 1474..1479 /label=-35 misc_feature 1480..1496 /label=lac O1 promoter 1497..1502 /label=-10 misc_feature 1455..1508 /label=BBa_R0011 promoter 1455..1508 /label=BBa_R0011 misc_feature 1522..1525 /label=conserved RBS 1518..1529 /label=BBa_B0034 CDS 1536..3398 /label=dxs CDS 1536..3398 /label=BBa_K1653000 misc_feature 3411..3414 /label=conserved RBS 3407..3418 /label=BBa_B0034 CDS 3425..3973 /label=idi CDS 3425..3973 /label=BBa_K1653002 misc_feature 3986..3989 /label=conserved RBS 3982..3993 /label=BBa_B0034 CDS 4000..5189 /label=ispDF CDS 4000..5189 /label=BBa_K1653001 stem_loop 5209..5252 /label=stem_loop misc_feature 5198..5277 /label=BBa_B0010 terminator 5198..5277 /label=BBa_B0010 stem_loop 5293..5312 /label=T7 TE misc_feature 5319..5319 /label=stop polyA_site 5313..5326 /label=polya misc_feature 5286..5326 /label=BBa_B0012 terminator 5286..5326 /label=BBa_B0012 terminator 5198..5326 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgggtaat gaaacggtgg tcgtggctga aacggctggt 121 tcgatcaaag ttgcggtcgt ggtctgcctg ctgcgtggtc aaaacgtgct gctgggtcgt 181 cgccgtagca gcctgggtga tagcaccttt agcctgccga gcggccatct ggaatttggt 241 gaaagctttg aagaatgcgc ggcccgcgaa ctgaaagaag aaaccgatct ggatattggc 301 aaaatcgaac tgctgaccgt gaccaacaac ctgtttctgg atgaagcgaa accgagccag 361 tatgtggccg tttttatgcg tgccgttctg gcggatccgc gtcaggaacc gcagaacatt 421 gaaccggaat tttgtgatgg ctggggttgg tatgaatggg ataatctgcc gaaaccgctg 481 ttctggccgc tggacaatgt ggttcaagac ggttttaatc cgttcccgac ctaatactag 541 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 601 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 661 ctgcgtttat atactagaga attgtgagcg gataacaatt gacattgtga gcggataaca 721 agatactgag cacatactag agaaagagga gaaatactag atgcaaacgg aacacgtcat 781 tttattgaat gcacagggag ttcccacggg tacgctggaa aagtatgccg cacacacggc 841 agacacccgc ttacatctcg cgttctccag ttggctgttt aatgccaaag gacaattatt 901 agttacccgc cgcgcactga gcaaaaaagc atggcctggc gtgtggacta actcggtttg 961 tgggcaccca caactgggag aaagcaacga agacgcagtg atccgccgtt gccgttatga 1021 gcttggcgtg gaaattacgc ctcctgaatc tatctatcct gactttcgct accgcgccac 1081 cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta tttgccgcac gcaccactag 1141 cgcgttacag atcaatgatg atgaagtgat ggattatcaa tggtgtgatt tagcagatgt 1201 attacacggt attgatgcca cgccgtgggc gttcagtccg tggatggtga tgcaggcgac 1261 aaatcgcgaa gccagaaaac gattatctgc atttacccag cttaaataat actagagcca 1321 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 1381 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 1441 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 1501 ctgagcacat actagagaaa gaggagaaat actagatgag ttttgatatt gccaaatacc 1561 cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac 1621 cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc 1681 acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca 1741 ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat aaaattttga 1801 ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt 1861 ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg 1921 ccggaattgg tattgcggtt gctgccgaaa aagaaggcaa aaatcgccgc accgtctgtg 1981 tcattggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat cacgcgggcg 2041 atatccgtcc tgatatgctg gtgattctca acgacaatga aatgtcgatt tccgaaaatg 2101 tcggcgcgct caacaaccat ctggcacagc tgctttccgg taagctttac tcttcactgc 2161 gcgaaggcgg gaaaaaagtt ttctctggcg tgccgccaat taaagagctg ctcaaacgca 2221 ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt gtttgaagag ctgggcttta 2281 actacatcgg cccggtggac ggtcacgatg tgctggggct tatcaccacg ctaaagaaca 2341 tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg 2401 aaccggcaga aaaagacccg atcactttcc acgccgtgcc taaatttgat ccctccagcg 2461 gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt 2521 tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag 2581 gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ctacttcgac gtggcaattg 2641 ccgagcaaca cgcggtgacc tttgctgcgg gtctggcgat tggtgggtac aaacccattg 2701 tcgcgattta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcga 2761 ttcaaaagct tccggtcctg ttcgccatcg accgcgcggg cattgttggt gctgacggtc 2821 aaacccatca gggtgctttt gatctctctt acctgcgctg cataccggaa atggtcatta 2881 tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg 2941 atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt cggcgtggaa ctgacgccgc 3001 tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggcgatcc 3061 ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg aacgccacgc 3121 tggtcgatat gcgttttgtg aaaccgcttg atgaagcgtt aattctggaa atggccgcca 3181 gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggcagcggcg 3241 tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggcctgccgg 3301 acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg 3361 ctggtatgga agccaaaatc aaggcctggc tggcataata ctagagaaag aggagaaata 3421 ctagatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct 3481 ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct ccagttggct 3541 gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa aagcatggcc 3601 tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca acgaagacgc 3661 agtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta 3721 tcctgacttt cgctaccgcg ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc 3781 ggtatttgcc gcacgcacca ctagcgcgtt acagatcaat gatgatgaag tgatggatta 3841 tcaatggtgt gatttagcag atgtattaca cggtattgat gccacgccgt gggcgttcag 3901 tccgtggatg gtgatgcagg cgacaaatcg cgaagccaga aaacgattat ctgcatttac 3961 ccagcttaaa taatactaga gaaagaggag aaatactaga tggcaaccac tcatttggat 4021 gtttgcgccg tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag 4081 caatatctct caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg 4141 catccccggg tgaaacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa 4201 cttcctctgg cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat 4261 tccgtgctgg caggtctgaa agccgctggc gacgcgcagt gggtattggt gcatgacgcc 4321 gctcgtcctt gtttgcatca ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc 4381 cgcacggggg ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc 4441 aaaaatgcca ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa 4501 tttttccctc gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgcgact 4561 attaccgacg aagcctcggc gctggaatat tgcggattcc atcctcagtt ggtcgaaggc 4621 cgtgcggata acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc 4681 acccgaacca tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacatg 4741 cctttggcgg tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat 4801 tgctggcgca ttctgatggc gacgtggcgc tccatgcgtt gaccgatgca ttgcttggcg 4861 cggcggcgct gggggatatc ggcaagctgt tcccggatac cgatccggca tttaaaggtg 4921 ccgatagccg cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc 4981 ttggcaacgt cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac 5041 aaatgcgcgt gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag 5101 ccactactac ggaaaaactg ggatttaccg gacgtgggga agggattgcc tgtgaagcgg 5161 tggcgctact cattaaggca acaaaatgat actagagcca ggcatcaaat aaaacgaaag 5221 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 5281 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //