##gff-version 3
##sequence-region BBa_K1653030 1 7607
BBa_K1653030	.	CDS	82	534	.	+	0	ID=BBa_K1653008;Name=BBa_K1653008
BBa_K1653030	.	CDS	82	547	.	+	0	ID=annotation2446619;Name=RhNUDX1;Parent=BBa_K1653008
BBa_K1653030	.	terminator	3599	3727	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653030	.	terminator	3687	3727	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	3687	3727	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653030	.	stem_loop	3694	3713	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653030	.	stop_codon	3720	3720	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653030	.	polyA_site	3714	3727	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653030	.	terminator	3599	3678	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	3599	3678	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653030	.	stem_loop	3610	3653	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653030	.	ribosome_entry_site	3799	3810	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653030	.	conserved_region	3803	3806	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653030	.	terminator	7479	7607	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653030	.	terminator	7567	7607	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	7567	7607	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653030	.	stem_loop	7574	7593	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653030	.	stop_codon	7600	7600	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653030	.	polyA_site	7594	7607	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653030	.	terminator	7479	7558	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	7479	7558	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653030	.	stem_loop	7490	7533	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653030	.	promoter	3736	3789	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653030	.	operator	3761	3777	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	operator	3738	3754	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	promoter	3778	3783	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653030	.	engineered_region	3736	3789	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653030	.	promoter	3755	3760	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653030	.	terminator	543	671	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1653030	.	terminator	631	671	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	631	671	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653030	.	stem_loop	638	657	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653030	.	stop_codon	664	664	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653030	.	polyA_site	658	671	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653030	.	terminator	543	622	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	543	622	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653030	.	stem_loop	554	597	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1653030	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653030	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653030	.	promoter	2610	2663	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653030	.	operator	2635	2651	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	operator	2612	2628	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	promoter	2652	2657	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653030	.	engineered_region	2610	2663	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653030	.	promoter	2629	2634	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653030	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1653030	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653030	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653030	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653030	.	ribosome_entry_site	2673	2684	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653030	.	conserved_region	2677	2680	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653030	.	CDS	2691	3590	.	+	0	ID=BBa_K1653004;Name=BBa_K1653004
BBa_K1653030	.	CDS	2691	3590	.	+	0	ID=annotation2445843;Name=m-ispA;Parent=BBa_K1653004
BBa_K1653030	.	ribosome_entry_site	6263	6274	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653030	.	conserved_region	6267	6270	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653030	.	ribosome_entry_site	5688	5699	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1653030	.	conserved_region	5692	5695	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653030	.	CDS	6281	7470	.	+	0	ID=BBa_K1653001;Name=BBa_K1653001
BBa_K1653030	.	CDS	6281	7470	.	+	0	ID=annotation2445940;Name=ispDF;Parent=BBa_K1653001
BBa_K1653030	.	CDS	5706	6254	.	+	0	ID=BBa_K1653002;Name=BBa_K1653002
BBa_K1653030	.	CDS	5706	6254	.	+	0	ID=annotation2432275;Name=idi;Parent=BBa_K1653002
BBa_K1653030	.	CDS	3817	5679	.	+	0	ID=BBa_K1653000;Name=BBa_K1653000
BBa_K1653030	.	CDS	3817	5679	.	+	0	ID=annotation2432264;Name=dxs;Parent=BBa_K1653000
BBa_K1653030	.	engineered_region	680	2601	.	+	0	ID=BBa_K1653021;Name=BBa_K1653021
BBa_K1653030	.	promoter	680	733	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K1653021
BBa_K1653030	.	operator	705	721	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	operator	682	698	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1653030	.	promoter	722	727	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1653030	.	engineered_region	680	733	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1653030	.	promoter	699	704	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1653030	.	CDS	761	2464	.	+	0	ID=BBa_K1653007;Name=BBa_K1653007;Parent=BBa_K1653021
BBa_K1653030	.	CDS	761	2470	.	+	0	ID=annotation2446618;Name=ObGES;Parent=BBa_K1653007
BBa_K1653030	.	ribosome_entry_site	743	754	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1653021
BBa_K1653030	.	conserved_region	747	750	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1653030	.	terminator	2473	2601	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1653021
BBa_K1653030	.	terminator	2561	2601	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	2561	2601	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1653030	.	stem_loop	2568	2587	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1653030	.	stop_codon	2594	2594	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1653030	.	polyA_site	2588	2601	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1653030	.	terminator	2473	2552	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1653030	.	engineered_region	2473	2552	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1653030	.	stem_loop	2484	2527	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1653030
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgggtaatgaaacggtggtcgtggctgaaacggctggttcgatcaaagttgcggtcgtggtctgcctgctgcgtgg
tcaaaacgtgctgctgggtcgtcgccgtagcagcctgggtgatagcacctttagcctgccgagcggccatctggaattt
ggtgaaagctttgaagaatgcgcggcccgcgaactgaaagaagaaaccgatctggatattggcaaaatcgaactgctga
ccgtgaccaacaacctgtttctggatgaagcgaaaccgagccagtatgtggccgtttttatgcgtgccgttctggcgga
tccgcgtcaggaaccgcagaacattgaaccggaattttgtgatggctggggttggtatgaatgggataatctgccgaaa
ccgctgttctggccgctggacaatgtggttcaagacggttttaatccgttcccgacctaatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgag
cggataacaagatactgagcacatactagagaaagaggagaaatactagatgtcttgtgctcgtattaccgttactctg
ccttatcgttctgctaaaactagcattcaacgcggtatcacccactatccggcgctgattcgtccacgttttagcgcgt
gcactccgctggcgagcgctatgccgctgtcctccacgccgctgatcaacggcgacaacagccagcgtaaaaacacccg
tcagcacatggaagagtcttcttctaagcgtcgcgaatacctgctggaagagactacccgtaaactgcaacgtaacgac
actgaatctgtagagaagctgaaactgatcgacaacatccagcagctgggcatcggttactatttcgaggatgcgatta
acgctgtcctgcgttctccgttctctactggtgaagaggacctgtttactgcggccctgcgtttccgtctgctgcgtca
caacggcatcgaaatttccccggaaatcttcctgaagttcaaggacgaacgcggcaaattcgacgaatccgatactctg
ggtctgctgagcctgtacgaagccagcaacctgggtgttgcgggcgaggaaatcctggaagaagcaatggagttcgcgg
aagcgcgcctgcgtcgctccctgagcgaacctgctgcacctctgcacggcgaagttgcacaagcactggacgttccgcg
tcacctgcgcatggctcgtctggaagcacgtcgtttcatcgaacaatatggtaaacaatccgaccacgatggcgacctg
ctggagctggcaatcctggattataatcaagtccaggcgcagcaccagtctgagctgaccgagatcatccgttggtgga
aagaactgggcctggttgataaactgtcttttggtcgtgatcgtccgctggaatgtttcctgtggactgtaggtctgct
gccagagcctaaatactcttccgtccgtatcgaactggctaaggctatttctatcctgctggtcattgatgatatcttc
gacacctacggcgagatggatgatctgatcctgttcaccgacgctatccgtcgttgggacctggaagcgatggaaggtc
tgccggaatacatgaagatttgctacatggccctgtataacactaccaacgaggtttgttacaaagtactgcgtgacac
gggccgtatcgttctgctgaatctgaagtccacctggattgatatgatcgagggtttcatggaggaagcgaagtggttc
aacggtggcagcgccccgaaactggaagaatacatcgaaaacggtgtgtccaccgctggtgcttacatggcctttgctc
atatcttcttcctgattggtgaaggtgtaacgcaccaaaactctcaactgtttacccagaaaccttatccgaaagtgtt
tagcgcagccggccgtattctgcgtctgtgggacgatctgggcaccgcgaaggaagaacaagaacgcggtgacctggca
agctgcgttcagctgttcatgaaagagaaatccctgaccgaggaagaagcacgttcccgcattctggaggagattaaag
gcctgtggcgtgacctgaacggtgaactggtttataacaaaaatctgccgctgtccattatcaaggtggccctgaacat
ggcacgcgcatcccaggtagtttataaacatgaccaggatacctatttctcctccgtagataactacgttgacgccctg
ttcttcactcaatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaata
ctagatggactttccgcagcaactcgaagcctgcgttaagcaggccaaccaggcgctgagccgttttatcgccccactg
ccctttcagaacactcccgtggtcgaaaccatgcagtatggcgcattattaggtggtaagcgcctgcgacctttcctgg
tttatgccaccggtcatatgttcggcgttagcacaaacacgctggacgcacccgctgccgccgttgagtgtatccacgc
ttactttttaattcatgatgatttaccggcaatggatgatgacgatctgcgtcgcggtttgccaacctgccatgtgaag
tttggcgaagcaaacgcgattctcgctggcgacgctttacaaacgctggcgttctcgattttaagcgatgccgatatgc
cggaagtgtcggaccgcgacagaatttcgatgatttctgaactggcgagcgccagtggtattgccggaatgtgcggtgg
tcaggcattagatttagacgcggaaggcaaacacgtacctctggacgcgcttgagcgtattcatcgtcataaaaccggc
gcattgattcgcgccgccgttcgccttggtgcattaagcgccggagataaaggacgtcgtgctctgccggtactcgaca
agtatgcagagagcatcggccttgccttccaggttcaggatgacatcctggatgtggtgggagatactgcaacgttggg
aaaacgccagggtgccgaccagcaacttggtaaaagtacctaccctgcacttctgggtcttgagcaagcccggaagaaa
gcccgggatctgatcgacgatgcccgtcagtcgctgaaacaactggctgaacagtcactcgatacctcggcactggaag
cgctagcggactacatcatccagcgtaataaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacata
ctagagaaagaggagaaatactagatgagttttgatattgccaaatacccgaccctggcactggtcgactccacccagg
agttacgactgttgccgaaagagagtttaccgaaactctgcgacgaactgcgccgctatttactcgacagcgtgagccg
ttccagcgggcacttcgcctccgggctgggcacggtcgaactgaccgtggcgctgcactatgtctacaacaccccgttt
gaccaattgatttgggatgtggggcatcaggcttatccgcataaaattttgaccggacgccgcgacaaaatcggcacca
tccgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagcgaatatgacgtattaagcgtcgggcattcatc
aacctccatcagtgccggaattggtattgcggttgctgccgaaaaagaaggcaaaaatcgccgcaccgtctgtgtcatt
ggcgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatcacgcgggcgatatccgtcctgatatgctggtga
ttctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctcaacaaccatctggcacagctgctttccggtaa
gctttactcttcactgcgcgaaggcgggaaaaaagttttctctggcgtgccgccaattaaagagctgctcaaacgcacc
gaagaacatattaaaggcatggtagtgcctggcacgttgtttgaagagctgggctttaactacatcggcccggtggacg
gtcacgatgtgctggggcttatcaccacgctaaagaacatgcgcgacctgaaaggcccgcagttcctgcatatcatgac
caaaaaaggtcgtggttatgaaccggcagaaaaagacccgatcactttccacgccgtgcctaaatttgatccctccagc
ggttgtttgccgaaaagtagcggcggtttgccgagctattcaaaaatctttggcgactggttgtgcgaaacggcagcga
aagacaacaagctgatggcgattactccggcgatgcgtgaaggttccggcatggtcgagttttcacgtaaattcccgga
tcgctacttcgacgtggcaattgccgagcaacacgcggtgacctttgctgcgggtctggcgattggtgggtacaaaccc
attgtcgcgatttactccactttcctgcaacgcgcctatgatcaggtgctgcatgacgtggcgattcaaaagcttccgg
tcctgttcgccatcgaccgcgcgggcattgttggtgctgacggtcaaacccatcagggtgcttttgatctctcttacct
gcgctgcataccggaaatggtcattatgaccccgagcgatgaaaacgaatgtcgccagatgctctataccggctatcac
tataacgatggcccgtcagcggtgcgctacccgcgtggcaacgcggtcggcgtggaactgacgccgctggaaaaactac
caattggcaaaggcattgtgaagcgtcgtggcgagaaactggcgatccttaactttggtacgctgatgccagaagcggc
gaaagtcgccgaatcgctgaacgccacgctggtcgatatgcgttttgtgaaaccgcttgatgaagcgttaattctggaa
atggccgccagccatgaagcgctggtcaccgtagaagaaaacgccattatgggcggcgcaggcagcggcgtgaacgaag
tgctgatggcccatcgtaaaccagtacccgtgctgaacattggcctgccggacttctttattccgcaaggaactcagga
agaaatgcgcgccgaactcggcctcgatgccgctggtatggaagccaaaatcaaggcctggctggcataatactagaga
aagaggagaaatactagatgcaaacggaacacgtcattttattgaatgcacagggagttcccacgggtacgctggaaaa
gtatgccgcacacacggcagacacccgcttacatctcgcgttctccagttggctgtttaatgccaaaggacaattatta
gttacccgccgcgcactgagcaaaaaagcatggcctggcgtgtggactaactcggtttgtgggcacccacaactgggag
aaagcaacgaagacgcagtgatccgccgttgccgttatgagcttggcgtggaaattacgcctcctgaatctatctatcc
tgactttcgctaccgcgccaccgatccgagtggcattgtggaaaatgaagtgtgtccggtatttgccgcacgcaccact
agcgcgttacagatcaatgatgatgaagtgatggattatcaatggtgtgatttagcagatgtattacacggtattgatg
ccacgccgtgggcgttcagtccgtggatggtgatgcaggcgacaaatcgcgaagccagaaaacgattatctgcatttac
ccagcttaaataatactagagaaagaggagaaatactagatggcaaccactcatttggatgtttgcgccgtggttccgg
cggccggatttggccgtcgaatgcaaacggaatgtcctaagcaatatctctcaatcggtaatcaaaccattcttgaaca
ctcggtgcatgcgctgctggcgcatccccgggtgaaacgtgtcgtcattgccataagtcctggcgatagccgttttgca
caacttcctctggcgaatcatccgcaaatcaccgttgtagatggcggtgatgagcgtgccgattccgtgctggcaggtc
tgaaagccgctggcgacgcgcagtgggtattggtgcatgacgccgctcgtccttgtttgcatcaggatgacctcgcgcg
attgttggcgttgagcgaaaccagccgcacgggggggatcctcgccgcaccagtgcgcgatactatgaaacgtgccgaa
ccgggcaaaaatgccattgctcataccgttgatcgcaacggcttatggcacgcgctgacgccgcaatttttccctcgtg
agctgttacatgactgtctgacgcgcgctctaaatgaaggcgcgactattaccgacgaagcctcggcgctggaatattg
cggattccatcctcagttggtcgaaggccgtgcggataacattaaagtcacgcgcccggaagatttggcactggccgag
ttttacctcacccgaaccatccatcaggagaatacataatgcgaattggacacggttttgacgtacatgcctttggcgg
tgaaggcccaattatcattggtggcgtacgcattccttacgaaaaaggattgctggcgcattctgatggcgacgtggcg
ctccatgcgttgaccgatgcattgcttggcgcggcggcgctgggggatatcggcaagctgttcccggataccgatccgg
catttaaaggtgccgatagccgcgagctgctacgcgaagcctggcgtcgtattcaggcgaagggttatacccttggcaa
cgtcgatgtcactatcatcgctcaggcaccgaagatgttgccgcacattccacaaatgcgcgtgtttattgccgaagat
ctcggctgccatatggatgatgttaacgtgaaagccactactacggaaaaactgggatttaccggacgtggggaaggga
ttgcctgtgaagcggtggcgctactcattaaggcaacaaaatgatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata