LOCUS       BBa_K1654013             486 bp    DNA     linear       30-Jun-2024
DEFINITION  Expression system for Hfq
ACCESSION   BBa_K1654013
VERSION     BBa_K1654013.1  
FEATURES             Location/Qualifiers
     promoter        1..59
                     /label=BBa_K1033225
     misc_feature    72..75
                     /label=conserved
     RBS             68..79
                     /label=BBa_B0034
     CDS             86..349
                     /label=BBa_K1654003
     stem_loop       369..412
                     /label=stem_loop
     misc_feature    358..437
                     /label=BBa_B0010
     terminator      358..437
                     /label=BBa_B0010
     stem_loop       453..472
                     /label=T7 TE
     misc_feature    479..479
                     /label=stop
     polyA_site      473..486
                     /label=polya
     misc_feature    446..486
                     /label=BBa_B0012
     terminator      446..486
                     /label=BBa_B0012
ORIGIN
        1 catcgggtag tttattcttg acaattaagt agagcctgat ataatagttc agtactgttt
       61 actagagaaa gaggagaaat actagatggc taaggggcaa tctctacaag acccattcct
      121 aaatgcgcta cgtcgtgagc gtatcccggt atctatctac cttgtgaacg gtatcaaact
      181 gcaaggtcag atcgaatctt tcgatcagtt cgtgatccta ttgaagaaca ctgttaacca
      241 aatggtgtac aagcacgcaa tctctaccgt tgttccggct cgtccagtga gccaccacag
      301 tggtgatcgt cctcaaggtg accgtccaca agagaaatct gaagattaat actagagcca
      361 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      421 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      481 tttata
//