LOCUS BBa_K1655000 4903 bp DNA linear 25-Nov-2024 DEFINITION Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli. ACCESSION BBa_K1655000 VERSION BBa_K1655000.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 misc_feature 22..43 /label=Lac RBS 76..82 /label=RBS CDS 90..555 /label=YciA RBS 579..585 /label=RBS CDS 593..1268 /label=Sfp RBS 1283..1289 /label=RBS CDS 1296..4821 /label=CAR misc_feature 4856..4903 /label=T7 double terminator ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt 61 tgtttaactt taagaaggag ataaaaaaaa tgtcggcaaa ctttaccgat aaaaacggac 121 gtcagtcaaa aggcgtctta ttattgcgca cgctggctat gcctagcgat acaaatgcca 181 acggcgacat tttcgggggg tggattatgt ctcagatgga catgggtggt gcgattcttg 241 ctaaagaaat tgcacatggc cgcgtggtta cagtggctgt cgaaagcatg aattttatca 301 agccgatttc cgtaggcgat gtagtgtgct gttacggtca gtgcctgaag gtggggcgca 361 gctcgattaa aattaaagtg gaggtatggg tcaaaaaggt ggcctcggaa cccattggcg 421 aacgttattg cgtgacggat gcggtattca cgtttgtcgc agtagataat aacggtcgta 481 gccggactat tccccgtgaa aacaaccagg aactggaaaa agcgcttgcg ttgatcagtg 541 agcagccact gtaataatta attaaaaagc ttggatccag gaggtaaaaa aaatgaaaat 601 ctacggcatc tatatggatc gtccgctgag ccaagaagaa aacgaacgtt ttatgagctt 661 tatcagtccg gaaaaacgtg aaaaatgccg tcgcttttat cacaaagaag atgcacatcg 721 taccctgctg ggtgatgttc tggttcgtag cgttattagc cgtcagtatc agctggataa 781 atccgatatt cgttttagca cccaagaata tggtaaaccg tgtattccgg atctgccgga 841 tgcacatttt aacattagcc atagcggtcg ttgggttatt tgtgcatttg atagccagcc 901 gattggcatt gatatcgaaa aaaccaaacc gatcagcctg gaaattgcca aacgtttttt 961 tagcaaaacc gagtatagcg atctgctggc caaagataaa gatgaacaga ccgattattt 1021 ctatcatctg tggtccatga aagagagctt cattaaacaa gaaggtaaag gtctgagcct 1081 gccgctggat agctttagcg ttcgtctgca tcaggatggt caggttagca ttgaactgcc 1141 ggatagccat agtccgtgtt atatcaaaac ctatgaagtt gaccctggct acaaaatggc 1201 agtttgtgca gcacatccgg attttccgga agatattacc atggttagct atgaagaact 1261 gctgtaataa ggatcccaat tcaggaggta aaaaaatgag tccgattaca cgcgaggaac 1321 gtctggagcg ccgtatccag gacctgtacg cgaacgatcc gcagtttgcc gcggccaagc 1381 cggcgaccgc gattacagcc gccatcgaac gccctggtct gccgcttccg cagatcattg 1441 aaaccgtgat gaccgggtac gcggatcgtc cagcgttggc gcagcgctcc gtcgagtttg 1501 tgactgatgc aggcacgggc catactacgc tgcggcttct gcctcacttt gaaacgattt 1561 cgtatggcga actttgggat cgcattagcg cattagcaga cgtcctgagc actgaacaga 1621 cggtcaaacc gggcgatcgc gtctgtcttc ttggcttcaa ctccgtcgat tatgcaacca 1681 tcgatatgac ccttgctcgc ctgggcgcag tcgccgtgcc cttgcagaca tccgctgcga 1741 ttacgcagct gcaaccaatc gtagctgaaa cccagccgac catgatcgct gcatctgtgg 1801 acgcactggc tgacgccaca gaactggcac ttagcggtca gacggcgacc cgcgtactgg 1861 tgtttgatca ccatcgtcag gttgacgcac atcgtgcggc ggttgaaagc gcgcgtgaac 1921 gtctggcagg ttctgcggtg gtggagacgt tggcagaggc gattgcacgc ggtgatgttc 1981 cgcggggcgc ctctgctggc tcggcgcctg gtactgatgt tagtgacgac agcctggcac 2041 tgctcatcta tacttcgggc agtacagggg ctccgaaagg cgcaatgtat ccgcgtcgta 2101 acgttgctac cttttggcgc aaacgtacgt ggtttgaagg tggttatgaa cctagcatca 2161 cgctgaactt catgcccatg agccatgtca tgggtcgtca aatcctttat gggaccctct 2221 gcaacggcgg tacagcgtac ttcgtagcga aatcggacct gagtacatta tttgaggacc 2281 tggccctggt acggccgacc gagctgactt ttgtgccacg cgtgtgggac atggtctttg 2341 acgagttcca gagtgaggtc gatcgccgtc tggtagatgg cgcagatcgt gttgcactgg 2401 aggcccaggt taaagcagaa attcggaatg acgttctcgg tggccgctac acgagcgcgc 2461 tgactggcag cgccccgatt agtgatgaga tgaaggcctg ggtggaggag ttactcgaca 2521 tgcatctggt agaaggctat ggcagcactg aggcgggcat gatcctgatc gacggcgcaa 2581 ttcgccgccc agccgttctg gactataaac tcgtcgacgt accggatctc ggctattttc 2641 tgaccgatcg tccgcaccca cgcggtgaat tgctggtgaa gaccgattcg ttgttcccgg 2701 gctattatca gcgggcagaa gtgacggcgg acgtgtttga cgccgatggc ttctatcgga 2761 caggcgatat tatggcggaa gtgggtcccg aacagttcgt ctatttggac cgtcgtaaca 2821 atgttctgaa gttaagtcag ggtgagttcg ttactgtttc aaaacttgag gcagtgtttg 2881 gcgattcacc gttagtccgc cagatctata tctatgggaa tagtgctcgc gcctacttac 2941 tcgccgtgat tgttccgaca caagaagccc tggatgcggt gcccgttgaa gaactcaagg 3001 cacggcttgg tgattcgctg caagaggtgg cgaaggcggc gggtctgcaa agctatgaaa 3061 ttccccgcga tttcatcatt gaaacgacgc cctggacgct tgaaaatggt ctgttgacgg 3121 gcatccgcaa attagcccgc ccgcagctca aaaaacacta tggggaactt ctggagcaaa 3181 tctataccga tctcgctcat ggtcaagcag atgagctgcg gtctttacgc cagagtggcg 3241 cggacgctcc ggtacttgtg accgtgtgtc gcgccgcagc ggcccttctg ggcggcagcg 3301 cgtcggatgt tcagccggat gctcatttta ctgaccttgg gggcgactct ctgagcgctc 3361 tgagctttac caacctgctg catgaaatct ttgatattga agtgccggtc ggcgtaatcg 3421 tttccccggc caacgatctg caagccttgg cggattatgt tgaagcggcc cgtaaacccg 3481 gcagctcacg cccgactttt gcgtcagtgc acggcgcatc caacggccag gtaacggaag 3541 ttcacgctgg ggacttgtcg cttgataaat ttattgatgc agcgacgctt gctgaagccc 3601 cacgcctgcc ggcggcgaat acccaggtcc gtacggttct gcttacaggc gccaccggat 3661 tcctgggccg ctacttagct ctggagtggc tcgagcgtat ggatctggtg gacggtaagc 3721 tgatttgtct ggtacgtgct aaatctgaca ccgaagcccg ggcacgtctg gacaaaacct 3781 ttgattcggg ggatcctgaa ctgctggcgc attatcgggc actggctggg gatcatttag 3841 aggtactggc gggcgacaag ggcgaagcgg accttggtct ggaccgccag acgtggcaac 3901 gcttagcgga taccgtggat ctgattgtgg atccggctgc gctggtgaac catgttctgc 3961 cgtactcaca attattcggc ccgaacgcac tggggaccgc cgaacttctc cgtcttgctt 4021 tgaccagcaa aatcaaaccc tacagctaca cctccaccat tggggttgca gatcaaattc 4081 ctccatccgc cttcaccgaa gacgcagaca ttcgcgtgat ttcagcaacc cgtgcagtgg 4141 atgattcata cgctaatggt tacagcaact ctaagtgggc tggtgaagtc ctgctgcgtg 4201 aagcacatga tctgtgtggt ctgcctgtag cggtattccg gtgtgatatg atcttggcgg 4261 ataccacctg ggccggtcag ttaaacgtgc cggatatgtt tacccgtatg attttgagcc 4321 tggctgcaac tggcatcgcc ccgggttcct tttatgaact tgcggcagat ggcgcacgtc 4381 aacgcgcaca ttacgatggt ctgccggtcg agtttattgc cgaagcgatc tctaccctgg 4441 gagcccagag tcaagatggc ttccatacat atcacgttat gaatccttac gatgacggta 4501 tcgggctgga tgaatttgtt gactggctga acgaatcggg ttgccccatc cagcgtatcg 4561 cagattatgg tgactggtta caacgttttg aaaccgccct gcgggcctta ccagatcgcc 4621 agcgtcacag ctcactgctg cctctgctgc ataactatcg tcaaccagaa cgccctgttc 4681 gcggcagcat tgccccgacc gaccgcttcc gcgctgccgt ccaggaagca aaaattggtc 4741 cggataaaga tattccgcat gttggtgcac ctattattgt caaatacgta agtgatttgc 4801 gcctgttagg tttattataa taattcccta ggctgctgcc accgctgagc aataactagc 4861 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttg //