BBa_K1655000 1 Propane1 Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli. 2015-09-13T11:00:00Z 2015-09-14T12:59:13Z The part sources are Haemophilus influenzae, Mycobacterium marinum (strain ATCC BAA-535 / M), and Bacillus subtilis for YciA, CAR and sfp, respectively. The promoter, RBS, and terminator sequences are derived from the commercial vector pCDF-Duet1 by Novagen. Propane 1 includes 1)acyl-CoA thioester hydrolase (YciA) from Haemophilus influenzae, which catalyzes the reaction: acyl-CoA + H2O <-> CoA + a carboxylate, 2) Carboxylic acid reductase (CAR) from Mycobacterium marinum (strain ATCC BAA-535 / M), which catalyzes the reaction: a carboxylate + reduced acceptor <-> an aldehyde + acceptor + H2O, and 3) maturation factor phosphopantetheinyl transferase (sfp) from Bacillus subtilis, which activates CAR by catalyzing the transfer of a prosthetic phosphopanteine group to CAR. All coding regions are assembled as an operon after a T7 promoter and and RBS from common cloning vectors (such as pCDF-Duet1 by Novagen). Terminator sequence after the last coding sequence, CAR is also derived from common cloning vectors (e.g. pCDF-Duet1 by Novagen uses this terminator). All coding sequences have been codon optimized twice for E. coli. First with Thermo Fisher's codon optimizer and then with IDT's codon optimization software. false false _2073_ 25169 25169 9 false Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed. false Annakarin Korppoo annotation2454837 1 RBS range2454837 1 1283 1289 annotation2454834 1 RBS range2454834 1 579 585 annotation2454838 1 CAR range2454838 1 1296 4821 annotation2454072 1 T7 range2454072 1 1 20 annotation2454073 1 Lac range2454073 1 22 43 annotation2454839 1 T7 double terminator range2454839 1 4856 4903 annotation2454832 1 YciA range2454832 1 90 555 annotation2454831 1 RBS range2454831 1 76 82 annotation2454836 1 Sfp range2454836 1 593 1268 BBa_K1655000_sequence 1 taatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaagaaggagataaaaaaaatgtcggcaaactttaccgataaaaacggacgtcagtcaaaaggcgtcttattattgcgcacgctggctatgcctagcgatacaaatgccaacggcgacattttcggggggtggattatgtctcagatggacatgggtggtgcgattcttgctaaagaaattgcacatggccgcgtggttacagtggctgtcgaaagcatgaattttatcaagccgatttccgtaggcgatgtagtgtgctgttacggtcagtgcctgaaggtggggcgcagctcgattaaaattaaagtggaggtatgggtcaaaaaggtggcctcggaacccattggcgaacgttattgcgtgacggatgcggtattcacgtttgtcgcagtagataataacggtcgtagccggactattccccgtgaaaacaaccaggaactggaaaaagcgcttgcgttgatcagtgagcagccactgtaataattaattaaaaagcttggatccaggaggtaaaaaaaatgaaaatctacggcatctatatggatcgtccgctgagccaagaagaaaacgaacgttttatgagctttatcagtccggaaaaacgtgaaaaatgccgtcgcttttatcacaaagaagatgcacatcgtaccctgctgggtgatgttctggttcgtagcgttattagccgtcagtatcagctggataaatccgatattcgttttagcacccaagaatatggtaaaccgtgtattccggatctgccggatgcacattttaacattagccatagcggtcgttgggttatttgtgcatttgatagccagccgattggcattgatatcgaaaaaaccaaaccgatcagcctggaaattgccaaacgtttttttagcaaaaccgagtatagcgatctgctggccaaagataaagatgaacagaccgattatttctatcatctgtggtccatgaaagagagcttcattaaacaagaaggtaaaggtctgagcctgccgctggatagctttagcgttcgtctgcatcaggatggtcaggttagcattgaactgccggatagccatagtccgtgttatatcaaaacctatgaagttgaccctggctacaaaatggcagtttgtgcagcacatccggattttccggaagatattaccatggttagctatgaagaactgctgtaataaggatcccaattcaggaggtaaaaaaatgagtccgattacacgcgaggaacgtctggagcgccgtatccaggacctgtacgcgaacgatccgcagtttgccgcggccaagccggcgaccgcgattacagccgccatcgaacgccctggtctgccgcttccgcagatcattgaaaccgtgatgaccgggtacgcggatcgtccagcgttggcgcagcgctccgtcgagtttgtgactgatgcaggcacgggccatactacgctgcggcttctgcctcactttgaaacgatttcgtatggcgaactttgggatcgcattagcgcattagcagacgtcctgagcactgaacagacggtcaaaccgggcgatcgcgtctgtcttcttggcttcaactccgtcgattatgcaaccatcgatatgacccttgctcgcctgggcgcagtcgccgtgcccttgcagacatccgctgcgattacgcagctgcaaccaatcgtagctgaaacccagccgaccatgatcgctgcatctgtggacgcactggctgacgccacagaactggcacttagcggtcagacggcgacccgcgtactggtgtttgatcaccatcgtcaggttgacgcacatcgtgcggcggttgaaagcgcgcgtgaacgtctggcaggttctgcggtggtggagacgttggcagaggcgattgcacgcggtgatgttccgcggggcgcctctgctggctcggcgcctggtactgatgttagtgacgacagcctggcactgctcatctatacttcgggcagtacaggggctccgaaaggcgcaatgtatccgcgtcgtaacgttgctaccttttggcgcaaacgtacgtggtttgaaggtggttatgaacctagcatcacgctgaacttcatgcccatgagccatgtcatgggtcgtcaaatcctttatgggaccctctgcaacggcggtacagcgtacttcgtagcgaaatcggacctgagtacattatttgaggacctggccctggtacggccgaccgagctgacttttgtgccacgcgtgtgggacatggtctttgacgagttccagagtgaggtcgatcgccgtctggtagatggcgcagatcgtgttgcactggaggcccaggttaaagcagaaattcggaatgacgttctcggtggccgctacacgagcgcgctgactggcagcgccccgattagtgatgagatgaaggcctgggtggaggagttactcgacatgcatctggtagaaggctatggcagcactgaggcgggcatgatcctgatcgacggcgcaattcgccgcccagccgttctggactataaactcgtcgacgtaccggatctcggctattttctgaccgatcgtccgcacccacgcggtgaattgctggtgaagaccgattcgttgttcccgggctattatcagcgggcagaagtgacggcggacgtgtttgacgccgatggcttctatcggacaggcgatattatggcggaagtgggtcccgaacagttcgtctatttggaccgtcgtaacaatgttctgaagttaagtcagggtgagttcgttactgtttcaaaacttgaggcagtgtttggcgattcaccgttagtccgccagatctatatctatgggaatagtgctcgcgcctacttactcgccgtgattgttccgacacaagaagccctggatgcggtgcccgttgaagaactcaaggcacggcttggtgattcgctgcaagaggtggcgaaggcggcgggtctgcaaagctatgaaattccccgcgatttcatcattgaaacgacgccctggacgcttgaaaatggtctgttgacgggcatccgcaaattagcccgcccgcagctcaaaaaacactatggggaacttctggagcaaatctataccgatctcgctcatggtcaagcagatgagctgcggtctttacgccagagtggcgcggacgctccggtacttgtgaccgtgtgtcgcgccgcagcggcccttctgggcggcagcgcgtcggatgttcagccggatgctcattttactgaccttgggggcgactctctgagcgctctgagctttaccaacctgctgcatgaaatctttgatattgaagtgccggtcggcgtaatcgtttccccggccaacgatctgcaagccttggcggattatgttgaagcggcccgtaaacccggcagctcacgcccgacttttgcgtcagtgcacggcgcatccaacggccaggtaacggaagttcacgctggggacttgtcgcttgataaatttattgatgcagcgacgcttgctgaagccccacgcctgccggcggcgaatacccaggtccgtacggttctgcttacaggcgccaccggattcctgggccgctacttagctctggagtggctcgagcgtatggatctggtggacggtaagctgatttgtctggtacgtgctaaatctgacaccgaagcccgggcacgtctggacaaaacctttgattcgggggatcctgaactgctggcgcattatcgggcactggctggggatcatttagaggtactggcgggcgacaagggcgaagcggaccttggtctggaccgccagacgtggcaacgcttagcggataccgtggatctgattgtggatccggctgcgctggtgaaccatgttctgccgtactcacaattattcggcccgaacgcactggggaccgccgaacttctccgtcttgctttgaccagcaaaatcaaaccctacagctacacctccaccattggggttgcagatcaaattcctccatccgccttcaccgaagacgcagacattcgcgtgatttcagcaacccgtgcagtggatgattcatacgctaatggttacagcaactctaagtgggctggtgaagtcctgctgcgtgaagcacatgatctgtgtggtctgcctgtagcggtattccggtgtgatatgatcttggcggataccacctgggccggtcagttaaacgtgccggatatgtttacccgtatgattttgagcctggctgcaactggcatcgccccgggttccttttatgaacttgcggcagatggcgcacgtcaacgcgcacattacgatggtctgccggtcgagtttattgccgaagcgatctctaccctgggagcccagagtcaagatggcttccatacatatcacgttatgaatccttacgatgacggtatcgggctggatgaatttgttgactggctgaacgaatcgggttgccccatccagcgtatcgcagattatggtgactggttacaacgttttgaaaccgccctgcgggccttaccagatcgccagcgtcacagctcactgctgcctctgctgcataactatcgtcaaccagaacgccctgttcgcggcagcattgccccgaccgaccgcttccgcgctgccgtccaggaagcaaaaattggtccggataaagatattccgcatgttggtgcacctattattgtcaaatacgtaagtgatttgcgcctgttaggtttattataataattccctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z