BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1658000
1
CAD1en
Cinnamyl alcohol dehydrogenase CAD1
2015-09-14T11:00:00Z
2015-09-18T04:48:19Z
Gene name: CAD1, Length: 1315 bp, Vector name: pUC57,
Sequence:
GAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTC
CTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGAT
GGGCTCGCTGGAAGTGGAACGTAAAACCGTGGGCTGGGCTGCTCG
TGACCCGTCTGGCGTGCTGTCCCCGTATGAATACACCCTGCGTAA
CACCGGCCCGCAGGATGTGTATGTTGAAGTCATGTGCTGTGGTAT
TTGTCATACGGATGTTCACCAAATCAAAAACGACCTGGGCATGAG
TAATTACCCGATGGTGCCGGGCCATGAAGTGGTTGGTGAAGTCGT
GGAAGTGGGCTCCGAAGTTACCAAATTTCGTGCCGGTGATGTTGT
CGGCGTCGGTTGTATTGTGGGCTCATGCGGTAACTGTCGCCCGTG
CAATTCGGATATTGAACAGTACTGCAACAAGAAAATTTGGAGTTA
CAACGATGTTTATCCGGATGGTAAACCGACCCAGGGCGGTTTTGC
AGGTGCTATGGTGGTTGATCAAAAATTCGTCGTGAAAATTCCGGA
TGGTATGGCCCCGGAACAGGCCGCCCCGCTGCTGTGCGCCGGTGT
GACGGTTTACAGCCCGCTGAATCATTTCGGCCTGAAACAATCTGG
TCTGCGCGGCGGTATCCTGGGTCTGGGCGGTGTTGGTCACATGGG
TGTGAAAATTGCGAAAGCGATGGGCCATCACGTCACCGTGATCAG
CTCTAGTGATAAAAAACGTGCAGAAGCTCTGGACCATCTGGGCGC
GGATGACTATCTGGTTTCCTCAGATGCAGCTCGCATGCAGGAAGC
GGCCGATTCTCTGGACTATATTATCGACACGGTTCCGGTCTTTCA
CCCGCTGGAACCGTACCTGAGCCTGCTGAAAATTGATGGCAAACT
GATCCTGATGGGTGTTGTCAACACCCCGCTGCAATTCGTTAGCCC
GATGGTCATGCTGGGTCGTAAATCTATTACGGGCAGTTTTATCGG
TTCCATGAAAGAACTGGCAGAAATGCTGGAATTTTGTAAAGAAAA
AGACCTGAGCAGCACCATCGAAATCGTGAAAATGGACTACATCAA
CACGGCTTTTGAACGCCTGGAAAAAAATGACGTGCGTTATCGCTT
CGTGGTGGATGTCGCGGGTAGCAAACTGTATCAATGATACTAGAG
CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCT
TTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTC
ACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAG
TAGCGGCCGCTGCAG,
Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) is an NADP(H) specific oxidoreductase catalysing the reversible conversion of cinnamyl aldehydes to the corresponding alcohols or monolignols (Mansel et al., 1974; Wyrambik and Griesebach, 1975; Boudet et al., 1995). Polymerization of the three monolignols: /?-coumaryl, sinapyl and coniferyl alcohols lead to lignin. The enzyme is closely related to lignification, and chemical inhibition of its activity reduces the synthesis of lignins (Moesbacher et al., 1990; Mauch-Mani and Slusarenko, 1996).
false
false
_2076_
16902
16902
9
Not in stock
true
.
false
Şeniz Y??ksel, Burak Kızıl
component2471000
1
BBa_J23100
component2471004
1
BBa_B0010
component2471002
1
BBa_B0034
component2471003
1
BBa_K1658006
annotation2471002
1
BBa_B0034
range2471002
1
44
55
annotation2471003
1
BBa_K1658006
range2471003
1
62
1143
annotation2471000
1
BBa_J23100
range2471000
1
1
35
annotation2471004
1
BBa_B0010
range2471004
1
1152
1231
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1658006
1
BBa_K1658006
CAD1 Enzyme
2015-09-14T11:00:00Z
2015-09-18T04:31:31Z
Ocimum basilicum is sweet basil (Eukaryota, Viridiplantae, Streptophyta, Embryophyta, Tracheophyta, Spermatophyta, Magnoliophyta, eudicotyledons, Gunneridae, Pentapetalae, asterids, lamiids, Lamiales, Lamiaceae,Nepetoideae, Ocimeae, Ocimum)
AY879285.1
Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) is an NADP(H) specific oxidoreductase catalysing the reversible conversion of cinnamyl aldehydes to the corresponding alcohols or monolignols (Mansel et al., 1974; Wyrambik and Griesebach, 1975; Boudet et al., 1995). Polymerization of the three monolignols: /?-coumaryl, sinapyl and coniferyl alcohols lead to lignin. The enzyme is closely related to lignification, and chemical inhibition of its activity reduces the synthesis of lignins (Moesbacher et al., 1990; Mauch-Mani and Slusarenko, 1996).
Involved in lignin biosynthesis. Catalyzes the final step specific for the production of lignin monomers. Catalyzes the NADPH-dependent reduction of coniferaldehyde, 5-hydroxyconiferaldehyde, sinapaldehyde, 4-coumaraldehyde and caffeyl aldehyde to their respective alcohols
false
false
_2076_
16902
16902
9
false
Gene name: CAD1
Length: 1315 bp
Vector name: pUC57
Sequence:
GAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTC
CTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGAT
GGGCTCGCTGGAAGTGGAACGTAAAACCGTGGGCTGGGCTGCTCG
TGACCCGTCTGGCGTGCTGTCCCCGTATGAATACACCCTGCGTAA
CACCGGCCCGCAGGATGTGTATGTTGAAGTCATGTGCTGTGGTAT
TTGTCATACGGATGTTCACCAAATCAAAAACGACCTGGGCATGAG
TAATTACCCGATGGTGCCGGGCCATGAAGTGGTTGGTGAAGTCGT
GGAAGTGGGCTCCGAAGTTACCAAATTTCGTGCCGGTGATGTTGT
CGGCGTCGGTTGTATTGTGGGCTCATGCGGTAACTGTCGCCCGTG
CAATTCGGATATTGAACAGTACTGCAACAAGAAAATTTGGAGTTA
CAACGATGTTTATCCGGATGGTAAACCGACCCAGGGCGGTTTTGC
AGGTGCTATGGTGGTTGATCAAAAATTCGTCGTGAAAATTCCGGA
TGGTATGGCCCCGGAACAGGCCGCCCCGCTGCTGTGCGCCGGTGT
GACGGTTTACAGCCCGCTGAATCATTTCGGCCTGAAACAATCTGG
TCTGCGCGGCGGTATCCTGGGTCTGGGCGGTGTTGGTCACATGGG
TGTGAAAATTGCGAAAGCGATGGGCCATCACGTCACCGTGATCAG
CTCTAGTGATAAAAAACGTGCAGAAGCTCTGGACCATCTGGGCGC
GGATGACTATCTGGTTTCCTCAGATGCAGCTCGCATGCAGGAAGC
GGCCGATTCTCTGGACTATATTATCGACACGGTTCCGGTCTTTCA
CCCGCTGGAACCGTACCTGAGCCTGCTGAAAATTGATGGCAAACT
GATCCTGATGGGTGTTGTCAACACCCCGCTGCAATTCGTTAGCCC
GATGGTCATGCTGGGTCGTAAATCTATTACGGGCAGTTTTATCGG
TTCCATGAAAGAACTGGCAGAAATGCTGGAATTTTGTAAAGAAAA
AGACCTGAGCAGCACCATCGAAATCGTGAAAATGGACTACATCAA
CACGGCTTTTGAACGCCTGGAAAAAAATGACGTGCGTTATCGCTT
CGTGGTGGATGTCGCGGGTAGCAAACTGTATCAATGATACTAGAG
CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCT
TTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTC
ACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAG
TAGCGGCCGCTGCAG
false
Şeniz Y??ksel
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1658006_sequence
1
atgggctcgctggaagtggaacgtaaaaccgtgggctgggctgctcgtgacccgtctggcgtgctgtccccgtatgaatacaccctgcgtaacaccggcccgcaggatgtgtatgttgaagtcatgtgctgtggtatttgtcatacggatgttcaccaaatcaaaaacgacctgggcatgagtaattacccgatggtgccgggccatgaagtggttggtgaagtcgtggaagtgggctccgaagttaccaaatttcgtgccggtgatgttgtcggcgtcggttgtattgtgggctcatgcggtaactgtcgcccgtgcaattcggatattgaacagtactgcaacaagaaaatttggagttacaacgatgtttatccggatggtaaaccgacccagggcggttttgcaggtgctatggtggttgatcaaaaattcgtcgtgaaaattccggatggtatggccccggaacaggccgccccgctgctgtgcgccggtgtgacggtttacagcccgctgaatcatttcggcctgaaacaatctggtctgcgcggcggtatcctgggtctgggcggtgttggtcacatgggtgtgaaaattgcgaaagcgatgggccatcacgtcaccgtgatcagctctagtgataaaaaacgtgcagaagctctggaccatctgggcgcggatgactatctggtttcctcagatgcagctcgcatgcaggaagcggccgattctctggactatattatcgacacggttccggtctttcacccgctggaaccgtacctgagcctgctgaaaattgatggcaaactgatcctgatgggtgttgtcaacaccccgctgcaattcgttagcccgatggtcatgctgggtcgtaaatctattacgggcagttttatcggttccatgaaagaactggcagaaatgctggaattttgtaaagaaaaagacctgagcagcaccatcgaaatcgtgaaaatggactacatcaacacggcttttgaacgcctggaaaaaaatgacgtgcgttatcgcttcgtggtggatgtcgcgggtagcaaactgtatcaatgatactagag
BBa_K1658000_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgggctcgctggaagtggaacgtaaaaccgtgggctgggctgctcgtgacccgtctggcgtgctgtccccgtatgaatacaccctgcgtaacaccggcccgcaggatgtgtatgttgaagtcatgtgctgtggtatttgtcatacggatgttcaccaaatcaaaaacgacctgggcatgagtaattacccgatggtgccgggccatgaagtggttggtgaagtcgtggaagtgggctccgaagttaccaaatttcgtgccggtgatgttgtcggcgtcggttgtattgtgggctcatgcggtaactgtcgcccgtgcaattcggatattgaacagtactgcaacaagaaaatttggagttacaacgatgtttatccggatggtaaaccgacccagggcggttttgcaggtgctatggtggttgatcaaaaattcgtcgtgaaaattccggatggtatggccccggaacaggccgccccgctgctgtgcgccggtgtgacggtttacagcccgctgaatcatttcggcctgaaacaatctggtctgcgcggcggtatcctgggtctgggcggtgttggtcacatgggtgtgaaaattgcgaaagcgatgggccatcacgtcaccgtgatcagctctagtgataaaaaacgtgcagaagctctggaccatctgggcgcggatgactatctggtttcctcagatgcagctcgcatgcaggaagcggccgattctctggactatattatcgacacggttccggtctttcacccgctggaaccgtacctgagcctgctgaaaattgatggcaaactgatcctgatgggtgttgtcaacaccccgctgcaattcgttagcccgatggtcatgctgggtcgtaaatctattacgggcagttttatcggttccatgaaagaactggcagaaatgctggaattttgtaaagaaaaagacctgagcagcaccatcgaaatcgtgaaaatggactacatcaacacggcttttgaacgcctggaaaaaaatgacgtgcgttatcgcttcgtggtggatgtcgcgggtagcaaactgtatcaatgatactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z