LOCUS       BBa_K1660004            1585 bp    DNA     linear       07-Dec-2025
DEFINITION  PcyA+Ho1
ACCESSION   BBa_K1660004
VERSION     BBa_K1660004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23100
     RBS             36..47
                     /label=B0034
     misc_feature    48..50
                     /label=start codon
     CDS             48..750
                     /label=pcyA
     misc_feature    792..797
                     /label=stop codon
     misc_feature    798..822
                     /label=pcyA barcode
     RBS             823..834
                     /label=B0034
     CDS             835..1560
                     /label=ho1
     misc_feature    835..837
                     /label=start codon
     misc_feature    1561..1585
                     /label=ho1 barcode
     misc_feature    1558..1560
                     /label=stop codon
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcaaaga ggagaaaatg gccgtcactg
       61 atttaagttt gaccaattct tccctgatgc ctacgttgaa cccgatgatt caacagttgg
      121 ccctggcgat cgccgctagt tggcaaagtt tacccctcaa gccctatcaa ttgccggagg
      181 atttgggcta cgtagaaggc cgcctggaag gggaaaagtt agtgattgaa aatcggtgct
      241 accaaacgcc ccagtttcgc aaaatgcatt tggagttggc caaggtgggc aaagggttgg
      301 atattctcca ctgtgtaatg tttcctgagc ctttatacgg tctacctttg tttggctgtg
      361 acattgtggc cggccccggt ggagtaagtg cggctattgc ggatctatcc cccacccaaa
      421 gcgatcgcca attgcccgca gcgtaccaaa aatcattggc agagctaggc cagccagaat
      481 ttgagcaaca acgggaattg cccccctggg gagaaatatt ttctgaatat tgtttattca
      541 tccgtcccag caatgtcact gaagaagaaa gatttgtaca aagggtagtg gactttttgc
      601 aaattcattg tcaccaatcc atcgttgccg aacccttgtc tgaagctcaa actttggagc
      661 accgtcaggg gcaaattcat tactgccaac aacaacagaa aaatgataaa acccgtcggg
      721 tactggaaaa agcttttggg gaagcttggg cggaacggta tatgagccaa gtcttatttg
      781 atgttatcca ataataactc tgatagtgct agtgtagatc tcaaagagga gaaaatgagt
      841 gtcaacttag cttcccagtt gcgggaaggg acgaaaaaat cccactccat ggcggagaac
      901 gtcggctttg tcaaatgctt cctcaagggc gttgtcgaga aaaattccta ccgtaagctg
      961 gttggcaatc tctactttgt ctacagtgcc atggaagagg aaatggcaaa atttaaggac
     1021 catcccatcc tcagccacat ttacttcccc gaactcaacc gcaaacaaag cctagagcaa
     1081 gacctgcaat tctattacgg ctccaactgg cggcaagaag tgaaaatttc tgccgctggc
     1141 caagcctatg tggaccgagt ccggcaagtg gccgctacgg cccctgaatt gttggtggcc
     1201 cattcctaca cccgttacct gggggatctt tccggcggtc aaattctcaa gaaaattgcc
     1261 caaaatgcca tgaatctcca cgatggtggc acagctttct atgaatttgc cgacattgat
     1321 gacgaaaagg cttttaaaaa tacctaccgt caagctatga atgatctgcc cattgaccaa
     1381 gccaccgccg aacggattgt ggatgaagcc aatgacgcct ttgccatgaa catgaaaatg
     1441 ttcaacgaac ttgaaggcaa cctgatcaag gcgatcggca ttatggtgtt caacagcctc
     1501 acccgtcgcc gcagtcaagg cagcaccgaa gttggcctcg ccacctccga aggctaataa
     1561 cgctgatagt gctagtgtag atcgc
//