LOCUS       BBa_K1662000            1929 bp    DNA     linear       07-May-2024
DEFINITION  Construct 1 Bicistronic:PelB-CDA under tetR and GFP under P(Lac)IQ 
            promoter
ACCESSION   BBa_K1662000
VERSION     BBa_K1662000.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=BBa_R0040 TetR Promoter 
     misc_feature    55..60
                     /label=scar
     RBS             88..102
                     /label=BBa_B0030 RBS 
     misc_feature    110..112
                     /label=Start codon
     misc_feature    113..175
                     /label=BBa_J32015 PelB 
     misc_feature    176..979
                     /label=Mature CDA2
     misc_feature    980..985
                     /label=Double stop codons
     promoter        986..1022
                     /label=BBa_I14032 P(Lac) IQ Promoter 
     misc_feature    1023..1028
                     /label=scar
     RBS             1056..1070
                     /label=BBa_B0030 RBS 
     misc_feature    1078..1080
                     /label=Start codon
     misc_feature    1081..1794
                     /label=eGFP
     misc_feature    1795..1800
                     /label=Double stop codons
     misc_feature    1801..1929
                     /label=BBa_B0015 Terminator
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atgactgcat gtgtaagagc aggccgtatt aaagaggaga aacgatgcaa tgaaatacct
      121 gctgccgacc gctgctgctg gtctgctgct cctcgctgcc cagccggcga tggccgcagc
      181 tacaaaaacg ccttttccgg attggcttag tgcatttacc gggttaaaag aatggcctgg
      241 gttagatcca ccttatatac ctttagattt cattgatttc agtcaaattc cagattataa
      301 ggaatatgat caaaaccatt gcgacagtgt tccaagggac tcgtgctctt tcgattgcca
      361 tcactgcacc gaacacgatg atgtgtacac atgttccaaa ctttcccaga catttgacga
      421 tggtccttct gcttccacta ctaaattatt ggaccggttg aagcataatt ccaccttctt
      481 caatttaggt gtcaatatag ttcaacatcc agatatctat caaagaatgc aaaaggaggg
      541 acacttaatc ggctcacata cctggtctca cgtatatttg ccaaatgtat cgaatgaaaa
      601 aattatagct caaattgaat ggtccatctg ggcgatgaat gctactggca accatacccc
      661 caaatggttc agacctccat atggcggaat agataataga gtaagagcaa taacaaggca
      721 atttggctta caagccgtct tatgggatca cgatactttt gattggagcc tccttctcaa
      781 tgattctgtc ataactgaac aagaaattct tcaaaatgta ataaactgga acaagtcagg
      841 aaccggatta atattagaac acgattcaac ggaaaaaact gtcgatcttg ccattaagat
      901 aaataagttg ataggtgatg atcaatcaac agtttctcat tgtgtcggcg gaattgatta
      961 cataaaagag ttcttgtcct aataatggtg caaaaccttt cgcggtatgg catgatagcg
     1021 cctactagat acgacagcac ccgctgtgtt attcaattaa agaggagaaa gccgattatg
     1081 gctagcaaag gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt
     1141 gatgttaacg gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1201 aaacttaccc tgaagttcat ctgcactact ggcaaactgc ctgttccatg gccaaccctg
     1261 gtcactactc tgtgctatgg tgttcaatgc ttttcaagat acccggatca tatgaaacgg
     1321 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaaggac catcttcttc
     1381 aaagatgacg gcaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1441 aatagaatcg agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa
     1501 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     1561 atcaaagtga acttcaagac ccgccacaac attgaagatg gaagcgttca actagcagac
     1621 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1681 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1741 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactgta caattaataa
     1801 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1861 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1921 gcgtttata
//