BBa_K1662000 1 BBa_K1662000 Construct 1 Bicistronic:PelB-CDA under tetR and GFP under P(Lac)IQ promoter 2015-08-25T11:00:00Z 2015-09-13T05:21:21Z saccharomyces cerevisiae- CDA2 mature protein, with a pelB sequence pelB tag for localization to the periplasm with Chitin deacetylase false false _2080_ 28764 19184 9 false We had to eliminate an illegal restriction site within the mature CDA protein. pel B was also problematic due to hairpin structures that were likely to form. We engineered these by base pair substitutions false Janet Standeven Jack Kwon annotation2453489 1 eGFP range2453489 1 1081 1794 annotation2453481 1 Start codon range2453481 1 110 112 annotation2453479 1 scar range2453479 1 55 60 annotation2453482 1 BBa_J32015 PelB range2453482 1 113 175 annotation2453485 1 BBa_I14032 P(Lac) IQ Promoter range2453485 1 986 1022 annotation2453491 1 BBa_B0015 Terminator range2453491 1 1801 1929 annotation2453488 1 Start codon range2453488 1 1078 1080 annotation2453478 1 BBa_R0040 TetR Promoter range2453478 1 1 54 annotation2453483 1 Mature CDA2 range2453483 1 176 979 annotation2453490 1 Double stop codons range2453490 1 1795 1800 annotation2453484 1 Double stop codons range2453484 1 980 985 annotation2453487 1 BBa_B0030 RBS range2453487 1 1056 1070 annotation2453480 1 BBa_B0030 RBS range2453480 1 88 102 annotation2453486 1 scar range2453486 1 1023 1028 BBa_K1662000_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgactgcatgtgtaagagcaggccgtattaaagaggagaaacgatgcaatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccgcagctacaaaaacgccttttccggattggcttagtgcatttaccgggttaaaagaatggcctgggttagatccaccttatatacctttagatttcattgatttcagtcaaattccagattataaggaatatgatcaaaaccattgcgacagtgttccaagggactcgtgctctttcgattgccatcactgcaccgaacacgatgatgtgtacacatgttccaaactttcccagacatttgacgatggtccttctgcttccactactaaattattggaccggttgaagcataattccaccttcttcaatttaggtgtcaatatagttcaacatccagatatctatcaaagaatgcaaaaggagggacacttaatcggctcacatacctggtctcacgtatatttgccaaatgtatcgaatgaaaaaattatagctcaaattgaatggtccatctgggcgatgaatgctactggcaaccatacccccaaatggttcagacctccatatggcggaatagataatagagtaagagcaataacaaggcaatttggcttacaagccgtcttatgggatcacgatacttttgattggagcctccttctcaatgattctgtcataactgaacaagaaattcttcaaaatgtaataaactggaacaagtcaggaaccggattaatattagaacacgattcaacggaaaaaactgtcgatcttgccattaagataaataagttgataggtgatgatcaatcaacagtttctcattgtgtcggcggaattgattacataaaagagttcttgtcctaataatggtgcaaaacctttcgcggtatggcatgatagcgcctactagatacgacagcacccgctgtgttattcaattaaagaggagaaagccgattatggctagcaaaggagaagaactcttcactggagttgtcccaattcttgttgaattagatggtgatgttaacggccacaagttctctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctgaagttcatctgcactactggcaaactgcctgttccatggccaaccctggtcactactctgtgctatggtgttcaatgcttttcaagatacccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaggaccatcttcttcaaagatgacggcaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgacttcaaggaagatggcaacattctgggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtgaacttcaagacccgccacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactgtacaattaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z