LOCUS       BBa_K1662001            2118 bp    DNA     linear       27-Dec-2024
DEFINITION  Construct 2 Bidirectional: 2 TetR promoters: PelB-CDA/eGFP
ACCESSION   BBa_K1662001
VERSION     BBa_K1662001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..129
                     /label=BBa_B0015 terminator
     misc_feature    130..135
                     /label=Double stop codons
     misc_feature    136..939
                     /label=Mature CDA2
     misc_feature    940..1002
                     /label=BBa_J32015 PelB
     misc_feature    1003..1005
                     /label=Start codon
     RBS             1013..1027
                     /label=BBa_B0030 RBS 
     misc_feature    1055..1060
                     /label=scar
     promoter        1061..1114
                     /label=BBa_R0040 TetR Promoter 
     misc_feature    1115..1157
                     /label=Multiple Cloning Site
     promoter        1158..1211
                     /label=BBa_R0040 TetR Promoter 
     misc_feature    1212..1217
                     /label=misc
     RBS             1245..1259
                     /label=BBa_B0030 RBS 
     misc_feature    1267..1269
                     /label=Start codon
     misc_feature    1270..1983
                     /label=eGFP
     misc_feature    1984..1989
                     /label=Double stop codons
     misc_feature    1990..2118
                     /label=BBa_B0015 terminator
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt tattaggaca agaactcttt tatgtaatca attccgccga cacaatgaga
      181 aactgttgat tgatcatcac ctatcaactt atttatctta atggcaagat cgacagtttt
      241 ttccgttgaa tcgtgttcta atattaatcc ggttcctgac ttgttccagt ttattacatt
      301 ttgaagaatt tcttgttcag ttatgacaga atcattgaga aggaggctcc aatcaaaagt
      361 atcgtgatcc cataagacgg cttgtaagcc aaattgcctt gttattgctc ttactctatt
      421 atctattccg ccatatggag gtctgaacca tttgggggta tggttgccag tagcattcat
      481 cgcccagatg gaccattcaa tttgagctat aattttttca ttcgatacat ttggcaaata
      541 tacgtgagac caggtatgtg agccgattaa gtgtccctcc ttttgcattc tttgatagat
      601 atctggatgt tgaactatat tgacacctaa attgaagaag gtggaattat gcttcaaccg
      661 gtccaataat ttagtagtgg aagcagaagg accatcgtca aatgtctggg aaagtttgga
      721 acatgtgtac acatcatcgt gttcggtgca gtgatggcaa tcgaaagagc acgagtccct
      781 tggaacactg tcgcaatggt tttgatcata ttccttataa tctggaattt gactgaaatc
      841 aatgaaatct aaaggtatat aaggtggatc taacccaggc cattctttta acccggtaaa
      901 tgcactaagc caatccggaa aaggcgtttt tgtagctgcg gccatcgccg gctgggcagc
      961 gaggagcagc agaccagcag cagcggtcgg cagcaggtat ttcataatcg gctttctcct
     1021 ctttaattga ataacacagc gggtgctgtc gtatatagta gtgctcagta tctctatcac
     1081 tgatagggat gtcaatctct atcactgata gggaaattcg agctcggtac ccggggatcc
     1141 cctagagtcg acctgcatcc ctatcagtga tagagattga catccctatc agtgatagag
     1201 atactgagca ctactatata cgacagcacc cgctgtgtta ttcaattaaa gaggagaaag
     1261 ccgattatgg ctagcaaagg agaagaactc ttcactggag ttgtcccaat tcttgttgaa
     1321 ttagatggtg atgttaacgg ccacaagttc tctgtcagtg gagagggtga aggtgatgca
     1381 acatacggaa aacttaccct gaagttcatc tgcactactg gcaaactgcc tgttccatgg
     1441 ccaaccctgg tcactactct gtgctatggt gttcaatgct tttcaagata cccggatcat
     1501 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaaggacc
     1561 atcttcttca aagatgacgg caactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1621 acccttgtta atagaatcga gttaaaaggt attgacttca aggaagatgg caacattctg
     1681 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1741 aagaatggaa tcaaagtgaa cttcaagacc cgccacaaca ttgaagatgg aagcgttcaa
     1801 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1861 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1921 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactgtac
     1981 aattaataac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2041 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2101 ggcctttctg cgtttata
//